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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Journal of Indonesian Tourism and Development Studies Biotropika Jurnal Al-Tadzkiyyah Journal of Tropical Biodiversity and Biotechnology Indonesian Journal of Chemistry Jurnal Analis Kesehatan Batoboh : Jurnal Pengabdian Pada Masyarakat Surya Abdimas Jurnal Biotek Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Ilmu dan Teknologi Kesehatan Grouper: Jurnal Ilmiah Perikanan Indonesian Journal of Global Health research Lumbung Farmasi : Jurnal Ilmu Kefarmasian Sciscitatio : Journal for Biological Science Jurnal Farmasi Bioinformatics and Biomedical Research Journal LEARNING : Jurnal Inovasi Penelitian Pendidikan dan Pembelajaran Idarah Tarbawiyah: Journal of Management in Islamic Education Jurnal Kajian Ilmu Pendidikan (JKIP) Journal of Educational Sciences Takuana: Jurnal Pendidikan, Sains, dan Humaniora Mestaka: Jurnal Pengabdian Kepada Masyarakat Journal of Biological Science and Education Indonesian Research Journal on Education QANUN: Journal of Islamic Laws and Studies Bengawan :Jurnal Pengabdian Masyarakat Journal of Research in Educational Management Rumphius Pattimura Biological Journal Jurnal Pengabdian kepada Masyarakat Arumbai Bioeksperimen Proceeding Of International Conference On Education, Society And Humanity Bionature Jurnal Pengabdian kepada Masyarakat Arumbai Halal Science
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The Invasive Plants Species along the Hiking Track of Mount Panderman Nature Tourism, Batu, East Java Septiadi, Luhur; Wahyudi, Didik; Rachman, Rhesma Sylvia; Syafrudin, Syafrudin; Alfaruqi, Nuri Thobibatus Shofia
Journal of Indonesian Tourism and Development Studies Vol. 6 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2018.006.01.08

Abstract

Indonesia has the highest biodiversity that places ecotourism as a sustainable development sector. The negative impact of ecotourism is the vulnerability to invasive plant species growth. They invade the habitat of the native plants and potentially alter the biodiversity balance. This invasive plant introduction is predicted caused by tourist and hiker visits. Therefore, the inventory of invasive plant species is urgently needed. The field survey was conducted on March 11-12, 2017 to inventory invasive plant species along the Panderman Mountain hiking track that common travelers explored and then identified. Species of Pinus Caribaea, Chromolaena odorata, Lantana camara, Leucaena leucocephala, Pennisetum purpureum are categorized invasive plants. Four species of plants otherwise potentially invasive include Calliandra calothrysus, Ageratum conyzoides, Bidens pilosa, and Acacia deccurens. The widespread access to protected environmental destinations leads to the spread of highly invasive species. The impact of invasive species depends on factors such as reproduction rate, vegetative phase, adaptability of seeds that can withstand adverse conditions and in particular human vectors as a way of spreading them.Keywords: Batu, Ecotourism, Hiking track, Invasive plants, Panderman Mountain.
Assessment of genetic diversity in EMS-induced porang (Amorphophallus muelleri Blume) revealed by RAPD markers Haritzah, Rendha Maulana; Radita, Novia Fitri; Ramadanti, Isma Salsabila; Azzahro, Yusri Despa; Wahyudi, Didik; Resmisari, Ruri Siti; Suyono
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15768

Abstract

Increasing genetic diversity of porang through mutation is urgently needed since porang has low genetic diveristy. Therefore, this study aims to increase the genetic diversity of porang using ethyl methanesulfonate (EMS) and analyze the genetic diversity of porang using RAPD markers. Porang explants were subjected to in vitro EMS induction at various concentrations (control, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and evaluated after 35 days. DNA amplification was carried out using 20 RAPD primers. Genetic diversity within and between porang populations was analyzed using POPGENE software, while cluster analysis was performed using PAST software to identify genetic diversity patterns. The results showed that EMS mutation induction effectively increased the genetic diversity of porang. Primers OPA-2 and OPA-9 were identified as the most effective primers for detecting genetic diversity resulting from mutation induction. The 0.2% EMS treatment is recommended because it produced the most notable genetic changes compared to the control and shows potential for generating superior mutants to support porang breeding programs.
Optimalisasi keaktifan belajar melalui model pembelajaran SSCS: Eksperimentasi di SMA Fatkurohmah, Diana; Gani, A; Wahyudi, Didik
Idarah Tarbawiyah: Journal of Management in Islamic Education Vol. 7 No. 2 (2026)
Publisher : Program Studi Magister Pendidikan Agama Islam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/itjmie.v7i2.23063

Abstract

Students’ learning activeness is one of the important indicators of the success of the learning process. However, the results of a preliminary study conducted at SMA Negeri 1 Jayapura, Ogan Komering Ulu Timur, indicate that the level of students’ learning activeness is still not optimal, particularly in the aspect of independently seeking information. This condition is caused by the learning process that still tends to be teacher-centered, resulting in limited student involvement in learning activities. This study aims to determine the effectiveness of the Search, Solve, Create, and Share (SSCS) learning model in improving students’ learning activeness in the subject of Islamic Religious Education. The study employed a quantitative approach using a quasi-experimental method with a post-test-only control group design. The sample consisted of 57 students divided into an experimental class and a control class. Data were collected through observation and a learning activeness questionnaire. The results of the instrument testing indicate that most of the statement items were valid, with a Cronbach’s Alpha reliability coefficient of 0.738, indicating a good level of reliability. The normality test using the Shapiro–Wilk test showed significance values of 0.126 for the experimental class and 0.776 for the control class (p > 0.05), indicating that the data were normally distributed, while the homogeneity test using Levene’s Test obtained a significance value of 0.061 (p > 0.05), indicating that the variances of the two groups were homogeneous. Furthermore, the results of the independent sample t-test showed a significance value of 0.000 < 0.05 with a t value of 7.406. Therefore, it can be concluded that the SSCS learning model is effective in improving students’ learning activeness in Islamic Religious Education learning at the senior high school level. Abstrak Keaktifan belajar peserta didik merupakan indikator penting dalam keberhasilan pembelajaran, namun di SMA Negeri 1 Jayapura Ogan Komering Ulu Timur masih belum optimal, terutama pada aspek mencari informasi secara mandiri. Hal ini disebabkan oleh proses pembelajaran yang masih cenderung berpusat pada guru sehingga keterlibatan siswa dalam kegiatan belajar menjadi terbatas. Penelitian ini bertujuan untuk mengetahui efektivitas model pembelajaran Search, Solve, Create, and Share (SSCS) terhadap keaktifan belajar peserta didik pada mata pelajaran Pendidikan Agama Islam. Penelitian ini menggunakan pendekatan kuantitatif dengan metode eksperimen semu (quasi-experimental design) melalui desain post-test only control group. Sampel penelitian berjumlah 57 peserta didik yang terdiri dari kelas eksperimen dan kelas kontrol. Teknik pengumpulan data dilakukan melalui angket keaktifan belajar. Hasil uji instrumen menunjukkan bahwa sebagian besar butir pernyataan dinyatakan valid dengan nilai reliabilitas Cronbach Alpha sebesar 0,738 yang menunjukkan tingkat keandalan yang baik. Uji normalitas menggunakan Shapiro-Wilk menunjukkan nilai signifikansi 0,126 pada kelas eksperimen dan 0,776 pada kelas kontrol (p>0,05) sehingga data berdistribusi normal, sedangkan uji homogenitas menggunakan Levene Test memperoleh nilai signifikansi 0,061 (p>0,05) yang menunjukkan varians kedua kelompok homogen. Hasil uji independent sample t-test menunjukkan nilai signifikansi 0,000 < 0,05 dengan nilai t sebesar 7,406. Dengan demikian, dapat disimpulkan bahwa model pembelajaran SSCS efektif dalam meningkatkan keaktifan belajar peserta didik.
The Effect of Variation in Light Spectra on the Growth of Chlorella Vulgaris and It is Ability to Decolorize RB5 Ummah, Faizatul; Saefi, Muhammad; Wahyudi, Didik
Bionature Vol. 26 No. 2 (2025): Oktober-Desember 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/6tkngw35

Abstract

Reactive Black 5 (RB5) is an azo dye widely used in the textile industry and improper processing of this dye can harmful to the aquatic environment, including limited light penetration and potential carcinogenic and mutagenic effects on aquatic organisms. Therefore, RB5 in waters needs to be handled seriously, one of which is through bioremediation efforts using Cholera vulgaris. Meanwhile, chlorella growth is highly dependent on various light spectrums. This study aimed to examine the effect of different light spectrums (red, blue, and white) on C. vulgaris cell density-based growth and its ability to decolorate RB5. A completely randomized design with three replications was applied using 30 mg/L RB5 solution, and was conducted for 12 days at laboratoty. Cell density was measured by a hemocytometer, and dye concentration was analyzed using a UV-Vis spectrophotometer. The results showed that blue light produced the highest algal growth, reaching 2.78 × 10⁷ cells/mL on the ninth day. Meanwhile, white light produced the greatest RB5 decolorization, reaching 80% reduction on the last day. These findings highlight the role of different light spectrums in optimizing algal cell density and dye removal efficiency.
Relationship Between Mudskipper (Periophthalmus spp.) Protein Content and Environmental Factors in CMC Tiga Warna Mangrove Fakhrul Rozy, Muhammad; Tyas Nyonita Punjungsari; Wahyudi, Didik
Grouper Vol. 17 No. 1 (2026): Grouper: Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v17i1.374

Abstract

Mudskipper is a brackish water fish with high protein content and potential as an alternative source of protein. One of its natural habitats is the Clungup Mangrove Conservation (CMC) Tiga Warna area. Therefore, this study aimed to analyze the protein content of mudskipper and examine its relationship with environmental factors and sediment organic carbon in the mangrove ecosystem. Samples of mudskipper (Periophthalmus gracilis and Periophthalmus modestus), environmental parameters, and organic carbon were collected using purposive sampling at five stations. Protein content was determined using the Lowry method, while organic carbon was analyzed using the Walkley-Black method. Data were analyzed using ANOVA, and relationships among variables were assessed using Principal Component Analysis (PCA). The results showed that protein content ranged from 11.55% to 18.55%. The highest pH was recorded at station 2 (7.437), temperature at station 1 (30.667°C), dissolved oxygen at station 3 (4.400 mg/L), salinity and total dissolved solids at station 5 (41 ppt; 27,833,333.33 ppm), and organic carbon at station 5 (1.995%). PCA indicated that protein content was positively associated with organic carbon and pH, but not with temperature, salinity, dissolved oxygen, or total dissolved solids. Furthermore, protein content did not significantly differ between species or sampling stations.
TIME TOKEN: MODEL PEMBELAJARAN DALAM MENINGKATKAN HASIL BELAJAR PESERTA DIDIK PADA MATA PELAJARAN PAI Yusnida, Azma; Makbuloh, Deden; Mustofa, Muhammad; Wahyudi, Didik
LEARNING : Jurnal Inovasi Penelitian Pendidikan dan Pembelajaran Vol. 6 No. 2 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia (P4I)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/learning.v6i2.10870

Abstract

ABSTRACT This study was motivated by the low academic achievement and lack of higher-order thinking skills among students in the Islamic Religious Education (IRE) course at SMP Negeri 1 Kota Agung Timur, where instruction is still dominated by one-way Direct Instruction. This situation has led to a lack of active student participation and suboptimal achievement in the cognitive domain at the C4 level. This study aims to test the effectiveness of the Time Token learning model on PAI learning outcomes. The study employs a quantitative approach using an experimental method and a post-test only control group design. The research sample consisted of 64 eighth-grade students selected through cluster sampling, comprising an experimental class (VIII D) that implemented the Time Token model and a control class (VIII F) that used the Direct Instruction model. The research instrument was a multiple-choice test based on Bloom’s Taxonomy, which had been tested for validity and reliability, with 16 items deemed suitable for use. Data were analyzed using normality and homogeneity tests, as well as the Mann–Whitney U hypothesis test. The results showed that the mean score of the experimental class was higher than that of the control class, and the majority of students in the experimental class had achieved the established Minimum Passing Score (MPS) of 76, whereas in the control class, there were still students who had not reached the MPS. Furthermore, the Asymp. Sig. (2-tailed) value < 0.05 indicates the presence of a significant. ABSTRAK Penelitian ini dilatarbelakangi oleh rendahnya hasil belajar dan kemampuan berpikir tingkat tinggi peserta didik pada mata pelajaran Pendidikan Agama Islam (PAI) di SMP Negeri 1 Kota Agung Timur yang masih didominasi oleh pembelajaran Direct Instruction yang bersifat satu arah. Kondisi tersebut berdampak pada kurangnya partisipasi aktif siswa serta belum optimalnya pencapaian ranah kognitif pada level C4. Penelitian ini bertujuan untuk menguji efektivitas model pembelajaran Time Token terhadap hasil belajar PAI. Penelitian menggunakan pendekatan kuantitatif dengan metode eksperimen dan desain post-test only control group design. Sampel penelitian berjumlah 64 siswa kelas VIII yang dipilih melalui teknik cluster sampling, terdiri atas kelas eksperimen (VIII D) yang menerapkan model Time Token dan kelas kontrol (VIII F) yang menggunakan model Direct Instruction. Instrumen penelitian berupa tes pilihan ganda berbasis Taksonomi Bloom yang telah diuji validitas dan reliabilitasnya, dengan 16 soal dinyatakan layak digunakan. Data dianalisis melalui uji normalitas, homogenitas, dan uji hipotesis Mann–Whitney U. Hasil penelitian menunjukkan bahwa nilai rata-rata kelas eksperimen lebih tinggi dibandingkan kelas kontrol, dan sebagian besar siswa pada kelas eksperimen telah mencapai Kriteria Ketuntasan Minimal (KKM) yang ditetapkan yaitu 76, sedangkan pada kelas kontrol masih terdapat siswa yang belum mencapai KKM. Selain itu, nilai Asymp. Sig. (2-tailed) < 0,05 menunjukkan adanya perbedaan signifikan antara kedua kelompok. Dengan demikian, model pembelajaran Time Token terbukti lebih efektif dalam meningkatkan hasil belajar PAI karena mampu mendorong keaktifan, pemerataan partisipasi, serta peningkatan kemampuan berpikir siswa pada semua level kognitif.
GAMBARAN PEMERIKSAAN REAL-TIME PCR HPV DNA DAN LIQUID BASED CYTOLOGY UNTUK DETEKSI DINI KANKER SERVIKS Rahmawati, Valentina Indah; Wahyudi, Didik
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar belakang: Kanker Servic merupakan salah satu penyebab kematian tertinggi pada wanita di Indonesia. Deteksi dini kanker serviks dapat dilakukan melalui pemeriksaan molekuler seperti RT-PCR Human Papilloma Virus (HPV) DNA dan pemeriksaan sitologi seperti Liquid Based Cytology (LBC). Kedua metode ini berperan penting dalam mendeteksi infeksi HPV yang merupakan penyebab utama kanker serviks. Tujuan: Penelitian ini bertujuan untuk mendapatkan hasil pemeriksaan molekuler RT-PCR HPV DNA dan pemeriksaan Liquid Based Cytology (LBC) pada wanita usia subur. Metode: Penelitian menggunakan desain deskriptif observasional, Melakukan uji sejumlah 55 sampel swab serviks wanita berusia 20-49 tahun yang menunjukkan hasil negatif dengan pemeriksaan LBC, kemudian dilakukan pemeriksaan RT-PCR HPV DNA untuk mendeteksi adanya infeksi HPV. Hasil: 55 sampel yang dinyatakan negatif lesi serviks melalui pemeriksaan Pap Smear dengan metode Liquid Based Cytology (LBC), terdeteksi adanya DNA Human Papillomavirus (HPV) pada 7 sampel (12,7%) menggunakan metode pemeriksaan molekuler Real Time-PCR. Simpulan: Ditemukan adanya perbedaan hasil pemeriksaan Human Papillomavirus dengan menggunakan HBV DNA dan dengan menggunakan metode LBC sebanyak 12,7%.
Evaluation of Pork-Specific DNA Primers for Halal Authentication in Processed Meats Salsabila, Aisyah Izmi Hamida; Wahyudi, Didik
Halal Science Volume 1 Issue 1
Publisher : ETFLIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/halal0101364

Abstract

Ensuring the authenticity of halal-labeled food products is a growing global concern, particularly due to recurring reports of adulteration with non-permissible components such as pork. Molecular techniques, especially polymerase chain reaction (PCR), offer a sensitive and specific means to detect pork DNA in processed meats, where traditional protein-based assays often fail due to denaturation. This study evaluates the sensitivity of five pork-specific mitochondrial DNA primers, ND5, Cytochrome B(1), Cytochrome B(2), PPA8, and Pork (F2/R1), for detecting Sus scrofa DNA in processed meat products using conventional PCR. Genomic DNA was extracted from corned pork and beef meatball samples and amplified using each primer under optimized conditions. Of the five primers tested, only PPA8, Pork (F2/R1), and Cytochrome B(2) produced clear, specific amplicons in pork samples, with no cross-reactivity observed in beef samples. PPA8 and Pork (F2/R1) demonstrated the strongest and most consistent amplification, suggesting superior sensitivity and reliability. ND5 and Cytochrome B(1) showed poor performance, indicating limited applicability in processed food matrices. These findings confirm the utility of selected primers in halal authentication and highlight the importance of empirical validation in primer selection. Future work should focus on expanding primer testing across diverse processed food types and incorporating quantitative PCR to establish detection thresholds.
Co-Authors A Gani, A A. Gani, A. Gani Afianti, Linda Dwi Afiati, Lely Agung, Rendyansah ahmad yani Aju Tjatur Nugroho Krisnaningsih Alfaruqi, Nuri Thobibatus Shofia Anak Agung Istri Sri Wiadnyani Andriani, Disa Annisa, Nenden Nur Anwar, Muhamad Syaeful Apita, Desma Hemaliyah Ardani, Edvi Gracia Ardy Prian Nirwana, Ardy Prian Auliantika, Dinda Helsa Azzahro, Yusri Despa Baharudin Baharudin Baharudin Bambang Hermawan Bayyinatul Muchtaromah Budhi, Dono Setya Chairul Amriyah Deden Makbuloh Dian Puspita Sari Dwi Agustina Dwi Gusmalawati Endang Sutariningsih Soetarto Endang Widyastuti Ervin Medianti Fadhila, Zulfa Nur Faizatul Ummah Fakhrul Rozy, Muhammad Fatkurohmah, Diana Fatoni, Ilham Fitriyah Fitriyah Gusmiati, Lutfiana Hasanah Hadiani, Dimas Pratidina Puriastuti Haritzah, Rendha Maulana Hasan, M. Nur Hidayatullah, Rafi Fuzan Ilmi, Zahrobatul Lil Indivia, Imey Tamara Isna Arofatun Nikmah, Isna Arofatun Juwanita, Agnes Kharisma, Viol Dhea Kusuma, Eka Wisnu Leondro, Henny Lia Hapsari M. Dimas Yudi Witjaksono Manshur, umar Mauludyah, Rohmatul Mimi Salmawati Mose, Windi Muhammad Mustofa Muhammad Saefi Mujahidin Ahmad Munandar, Hanozanor Fadli Nawang, Pungky Nisa, Kurnia Khoirun NURUL AZIZAH Oke Anandika Lestari Pratimasari, Diah Prihandini, Peni Wahyu PUDJI ASTUTI Punjungsari, Tyas Nyonita Purwandani, Safitri Putri, Sheli Feliyan Rachman, Rhesma Sylvia Radita, Novia Fitri Rahmawati, Valentina Indah Ramadanti, Isma Salsabila Rodiyati Azrianingsih Ruri Siti Resmisari Rusdi, Nurhidaya Salsabila, Aisyah Izmi Hamida Saputra, Dedech Muhammad Sari, Retno Komala sari, Sonya Permata Saroh, Dewi Septiadi, Luhur Septiani, Risma Dyah Setya, Adhi Kumoro Setyazi, Gama Stefanus Khrismasagung Trikusumaadi, Stefanus Khrismasagung Susilowati Susilowati SUYONO Suyono Suyono Syafe’I, Imam Syafrudin Syafrudin Syahran Wael Umi Fathonah, Yuliana Umi Hijriyah Valent, Febriana Verawati, Liesta Wasehudin, Wasehudin Widyastuti, Dinda Yuli Arif Tribudi Yusianti Silviani Yusnida, Azma Zahra, Desti