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EPIDIDIMIS DAN PERANNYA PADA PEMATANGAN SPERMATOZOA Muslim Akmal; Dian Masyitah; Hafizuddin Hafizuddin; Fitriani Fitriani
JESBIO : Jurnal Edukasi dan Sains Biologi Vol 4 No 2 (2015): Jurnal Edukasi dan Sains Biologi
Publisher : Program Studi Biologi Fakultas Keguruan dan Ilmu Pendidikan Universitas Almuslim

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Abstract

Epididymis is the important organ in male reproductive system. Functions of epididymis are as transportation, maturation, and storage of sperm. Sperm of testikular are non-functional gamets, and only during transit through the epididymis, the sperm will become functional gamets. Synthesis and secretion of some proteins by epididymis epithelium are very important for maturation of sperm in epididymis. The objective is to know the important role of epididymis in secretion of some proteins or molecule and its function for sperm maturation. Keywords : Epididymis, spermatogenesis, protein
VISUALISASI LENDIR MUKOSA OS CERVICALIS EXTERNAL SAPI ACEH SEJAK AWAL BERAHI MENGGUNAKAN ALAT INSEMINASI BUATAN (IB) BERKAMERA (VISUALIZATION OF EXTERNAL CERVICALIS MUCOSAL MUCOSE OS IN ACEH CATTLE SINCE THE BEGINNING OESTRUS USING CAMERA EQUIPPED ARTIFICIAL INSEMINATION TOOL) Fahrul Brinaldi; Ginta Riady; Cut Nila Thasmi; Hafizuddin Hafizuddin; M Jalaluddin; Nurliana Nurliana
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 2 (2022): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i2.17744

Abstract

ABSTRAKPermasalahan yang terjadi pada peternak sapi di Indonesia adalah rendahnya produktifitas dan mutu genetik yang disebabkan oleh minimnya penggunaan teknologi dan kurangnya keterampilan peternak saat proses Inseminasi Buatan (IB). Metode rektovaginal yang umum digunakan dalam proses IB saat ini memiliki resiko kesalahan dalam penempatan deposisi semen pada organ reproduksi betina. Tujuan penelitian untuk mengetahui visualisasi dari os cervicalis external selama fase berahi sapi betina dengan menggunakan alat IB berkamera. Penelitian ini menggunakan 12 ekor sapi berumur 4-6 tahun yang disinkorinasi berahi dengan prostaglandin. Deteksi berahi dilakukan tiga kali yaitu pagi; siang dan sore dengan lama deteksi 30 menit. Penetapan berahi sampel sapi berdasarkan total skor tingkah laku berahi. Sapi betina berahi kemudian diperiksa dengan alat IB berkamera untuk mendapatkan visualisasi os cervicalis external.  Parameter yang diamati adalah visualisasi os cervicalis external dan kualitas lendir mukosa cervix  pada 0; 6 dan 12 jam setelah berahi. Hasil penelitian menunjukkan lendir mukosa cervix pada 8 ekor sapi saat pengamatan 0 dan 6 jam berahi tampak transparan. Sedangkan pengamatan pada 12 jam setelah berahi, 5 ekor sapi (63%) memperlihatkan lendir mukosa cervix transparan dan 3 ekor sapi (37%) memperlihatkan lendir mukosa cervix  kental. Dapat disimpulkan bahwa visualisasi os cervicalis external  umumnya memperlihatkan lendir mukosa cervix transparan pada 0; 6 dan 12 jam setelah berahi.                                         Kata kunci : Alat Inseminasi Buatan (IB) Berkamera, Visualisasi, Mukosa. ABSTRACT      Problems that occur in cattle breeders in Indonesia are low productivity and genetic quality which were associated with the lack of use of technology and lack of skills of farmers during the artificial insemination process. The rectovaginal method which is commonly used in artificial insemination today has a risk of error in the placement of semen deposition in the female reproductive organs. The purpose of the study was to determine the visualization of the os external cervical mucus during the oestrus phase of female cows using a camera- equipped artificial insemination tool. This study used 12 cows aged 4-6 years old which were synchronised using prostaglandin. Oestrus detection was performed thrice a day namely in the morning; noon and late afternoon; for thirty minutes, respectively. Determination of estrus status of samples based on the total scores of oestrous behavior. Parameters observed were visualization of the external cervical os and the quality of cervical mucus at 0; 6 and 12 hours after estrus. The results showed cervical mucus in 8 cows during observation at 0 and  6 hours after estrus looked transparent. Where as examination at 12 hours after oestrus,  cervical mucus were transparent and cloudy for 5 samples (63%) and for other 3 sampels (27%), respectively. It can be concluded that visualization of the os external cervical mucus of local cows generally showed  transparent at 0; 6 and 12 hours after oestrus.Key words : Artificial Insemination Tool, Visualization, Mucosa. 
HORMON DAN PERANNYA DALAM DINAMIKA FOLIKULER PADA HEWAN DOMESTIK Hafizuddin .; Tongku N Siregar; Muslim Akmal
JESBIO : Jurnal Edukasi dan Sains Biologi Vol 1 No 1 (2012): Jurnal Edukasi dan Sains Biologi
Publisher : Program Studi Biologi Fakultas Keguruan dan Ilmu Pendidikan Universitas Almuslim

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Abstract

Dinamika folikuler atau sering disebut dengan gelombang folikuler merupakan suatu perkembangan folikel (folikulogenesis) meliputi pertumbuhan serentak sekelompok folikel, satu diantaranya akan menjadi folikel dominan, mencapai ukuran terbesar, serta akan menekan perkembangan folikel-folikel lain. Folikulogenesis adalah proses yang bertanggung jawab untuk perkembangan folikel ovulatori dan pelepasan satu atau lebih oosit pada interval tertentu pada keseluruhan siklus reproduksi hewan betina. Jumlah gelombang per siklus tidak tergantung pada usia atau breed hewan. Gelombang folikel terdiri dari kelompok folikel yang mengalami perkembangan serentak selama fase akhir pertumbuhan. Tujuan penulisan ini adalah untuk mengetahui hormon apa saja yang terlibat dalam proses tersebut serta peranannya. Dinamika folikuler di negara maju sudah di aplikasikan dalam memantau fertilitas dan manipulasi reproduksi, sehingga dengan pengetahuan tersebut juga akan dapat menambah pengetahuan fungsi ovarium pada hewan.Kata kunci : hormon, dinamika folikuler, hewan domestic
EPIDIDIMIS DAN PERANNYA PADA PEMATANGAN SPERMATOZOA Muslim Akmal; Dian Masyitah; Hafizuddin Hafizuddin; Fitriani Fitriani
JESBIO : Jurnal Edukasi dan Sains Biologi Vol 4 No 2 (2015): Jurnal Edukasi dan Sains Biologi
Publisher : Program Studi Biologi Fakultas Keguruan dan Ilmu Pendidikan Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.009 KB)

Abstract

Epididymis is the important organ in male reproductive system. Functions of epididymis are as transportation, maturation, and storage of sperm. Sperm of testikular are non-functional gamets, and only during transit through the epididymis, the sperm will become functional gamets. Synthesis and secretion of some proteins by epididymis epithelium are very important for maturation of sperm in epididymis. The objective is to know the important role of epididymis in secretion of some proteins or molecule and its function for sperm maturation. Keywords : Epididymis, spermatogenesis, protein
Adiponectin and Testosterone Levels and Their Correlations with Fertility in Anglo-Nubian x Etawah Grade Crossbred Bucks Hafizuddin Hafizuddin; N. W. K. Karja; L. Praharani; M. A. Setiadi
Tropical Animal Science Journal Vol. 43 No. 2 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.894 KB) | DOI: 10.5398/tasj.2020.43.2.110

Abstract

The purpose of the current study was to determine the concentrations of adiponectin and testosterone in various ages of bucks as well as the correlations of adiponectin and testosterone with semen quality as an indicator of fertility. Nineteen Anglo Nubian (AN) x Etawah Grade (PE) (Anpera) crossbred bucks belonged to the Research Institute for Animal Production were classified into four different age groups: 24 months, 30 months, 36 months, and more than 48 months. The concentrations of adiponectin and testosterone in the samples of blood plasma were analyzed using enzyme-linked immunosorbent assay (ELISA). The semen characteristics were evaluated both macroscopically and microscopically. The results of the study showed that plasma concentrations of adiponectin in Anpera bucks at the ages of 24, 30, 36, and more than 48 months were 14.10±1.73 mg/L, 18.36±8.25 mg/L, 20.38±8.67 mg/L, and 15.41±7.31 mg/L, respectively. There was no significant difference in adiponectin concentrations among the age groups (p>0.05). However, there were correlations of adiponectin concentrations with semen volume, sperm morphology, and sperm concentration. Plasma concentrations of testosterone at the ages of 24, 30, 36, and more than 48 months were 9.76±2.46 ng/mL, 9.81±1.56 ng/mL, 10.05±0.94 ng/mL, and 9.93±2.71 ng/mL, respectively. Plasma concentrations of testosterone in Anpera bucks also did not demonstrate a significant difference among the age groups (p>0.05). However, plasma testosterone concentrations had significant correlations with semen volume, sperm motility, and sperm concentration. In conclusion, the plasma concentrations of adiponectin and testosterone hormones had correlations with the semen quality parameters, which are related to fertility.
Penambahan PGF2α dalam pengencer semen dapat meningkatkan motilitas pasca thawing motility spermatozoa domba Waringin Husnurrizal Husnurrizal; Ade Syahriani Aritonang; Tongku Nizwan Siregar; Teuku Armansyah; Hafizuddin Hafizuddin
Livestock and Animal Research Vol 19, No 2 (2021): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.28 KB) | DOI: 10.20961/lar.v19i2.49186

Abstract

Objective: The purpose of this research was to find out the rise of spermatozoa motility of Waringin sheep which was given additional amount of PGF2α in commercial semen diluents (Andromed).Methods: A total of 3 Waringin sheep aged 2-3 years were used in this study. Semen samples were collected using an artificial vagina and evaluation of the quality of the spermatozoa was carried out macroscopically and microscopically. This study used a unidirectional completely randomized design. After evaluated, semen samples were added with Andromed diluents afterwards divided into three groups treatment, K1; K2; and K3 where each group was then added with physiologic NaCl, 37,5 µg PGF2α, and 75 µg PGF2α. All of the samples were equilibrated in refrigerator for 4 hours and spermatozoa motility was examined, then after the freezing process was carried out and stored for 24 hours, the post thawing spermatozoa motility was examined. The data obtained was analyzed using one-way analysis of variance (ANOVA) and followed by Duncan test.Results: The result of the research showed that motility of post equilibration spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 43,67±1,20; 75,00±4,00; and 82,33 ±3,18 (P<0,05); and motility of post thawing spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 25,33±1,85; 49,33±3,17; dan 54,00 ±5,50 (P<0,05).Conclusions: In conclusion that was the rise in motility of prefreezing spermatozoa and motility of post thawing spermatozoa of Waringin sheep which given additional amount of PGF2α on Andromed diluents.
Efek pemberian ekstrak pituitari sapi terhadap peningkatan jumlah folikel ovarium dan konsentrasi estradiol pada tikus putih (Rattus norvegicus) Indah Kesuma Siregar; Tongku Nizwan Siregar; Muslim Akmal; Sri Wahyuni; Nazaruddin Nazaruddin; Hafizuddin Hafizuddin
Livestock and Animal Research Vol 18, No 2 (2020): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.121 KB) | DOI: 10.20961/lar.v18i2.42956

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Identification of factors causing reproductive disorders of the cow found in North Labuhanbatu Regency, North Sumatera Province Muharriza Nasution; Tongku Nizwan Siregar; Arman Sayuti; Hafizuddin Hafizuddin; Rosmaidar Rosmaidar; Mulyadi Adam
Livestock and Animal Research Vol 19, No 1 (2021): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.692 KB) | DOI: 10.20961/lar.v19i1.41766

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Objective: This study aims to identify the factors that cause reproductive disorders in beef cattle in the North Labuhanbatu, North Sumatra.Methods: The objects of this study were cattle with reproductive disorders and breeders whose cattle had reproductive disorders. This research used a survey method. Primary data was obtained by distributing questionnaires and conducting direct interviews with breeders, while secondary data was obtained by studying the 2017 report of UPSUS SIWAB (Indonesian Ministry of Agriculture's program to accelerate the fulfilment of domestic beef cattle population targets) in North Labuhanbatu Regency. The questionnaire covered the characteristics of cow with reproductive disorders including body condition score (BCS), type of cows, feed given to cows with reproductive disorders, maintenance management, veterinary/medical personnel visits, pregnancy checks, heat synchronization, birth assistance, type of mating, and the breeders identity. This questionnaire was distributed to 30 breeder respondents in 5 districts. Data were analyzed using stepwise regression with SPSS.Results: The results showed that 110 cows had reproductive disorders, 57.3% of which experienced hypofunctional ovaries. The independent variable that has the strongest correlation to the dependent variable is the body condition score (sig. 0.043), followed by the feed given to the cows (sig. 0.046) with the regression equation Y = -4.297 + 1.495X1 + 2.492X2.Conclusions: The identified factors that had correlation to reproductive disorders in female beef cattle are body condition score and feed.
Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali Amalia Sutriana; Tongku Nizwan Siregar; Feby Claudia Sirait; Juli Melia; Hafizuddin .
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.65067

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Penelitian ini bertujuan mengetahui pengaruh pemberian gonadotropin releasing hormone (GnRH) terhadap peningkatan kualitas spermatozoa sapi bali. Penelitian ini menggunakan dua ekor sapi bali berumur 2 dan 4 tahun dengan rancangan pola bujursangkar latin 2 perlakuan dan 2 periode perlakuan. Perlakuan yang dilakukan adalah P1 (NaCl fisiologis) dan P2 (GnRH 100 µg) dengan interval periode waktu perlakuan adalah satu minggu. Sampel semen dikoleksi menggunakan vagina buatan tiga hari setelah perlakuan. Data hasil pemeriksaan kualitas spermatozoa sapi bali dianalisis dengan uji Wilcoxon Signed Ranks Test. Berdasarkan uji statistik menunjukkan bahwa pemberian GnRH 100 µg tidak berpengaruh nyata (P>0,05) terhadap pemeriksaan warna, pH, konsistensi, volume, konsentrasi dan motilitas spermatozoa sapi bali. Hasil pemeriksaan menunjukkan bahwa warna semen pada kedua perlakuan adalah krem dan putih susu. Derajat keasaman (pH); konsistensi; volume semen (ml); konsentrasi (10⁶/ml); dan motilitas spermatozoa (%) sapi  bali pada P1 vs P2 adalah 7,0±0,0 vs 7,0±0,0; 2,5±0,71 vs 3,0±0,0; 4,5±2,8 vs 4,8±2,8; 925±106,07 vs 1210±155,56; dan 69,0±1,41 vs 71,5±0,71 (P>0,05). Disimpulkan bahwa pemberian GnRH 100 µg tidak memengaruhi kualitas spermatozoa sapi bali.
Comparison of reproductive performance of Gembrong goats and male Boerka goats Husnurrizal Husnurrizal; Daffa Gustia Putra Akbar; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Sri Wahyuni; Anwar Anwar; Arie Febretrisiana
Livestock and Animal Research Vol 21, No 1 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i1.63859

Abstract

Objective: This study aims to compare the reproductive performance of Boerka goats and Gembrong goats.Methods: The research was conducted at the Sei Putih Slaughter Goat Research Workshop in Deli Serdang, North Sumatra. In this study, male Boerka (n=3) and Gembrong (n=3) goats, aged 3 years and having the same average Body Condition Score (BCS) of 3.83±0.29, were used. Reproductive performance observed included scrotal circumference, libido score, and semen quality macroscopically (volume, color, odor, and consistency) and microscopically (concentration, motility, viability, and abnormalities). The data collected was tabulated and analyzed by t test.Results: Of all the parameters of reproductive performance examined, it is known that only the parameters of semen volume (ml) and sperm concentration (x106 cells/ml) showed a significant difference (P<0.05) between Gembrong goats vs. Boerka goats with values of 0.50±0.0 vs. 1.0±0.20 and 1.557±712 vs. 4.500±317.65, respectively, while the other parameters did not show a significant difference (P>0.05).Conclusions: It was concluded that the volume and concentration of Boerka goats were higher than Gembrong goats.
Co-Authors Ade Syahriani Aritonang Adhea Prestiya Ahmad Baihaqi Ahsan, Muhammad Maulana Aisyah Fadillah Tunnisa Amalia Sutriana Amalia Sutriana Amalia Sutriana Anwar Anwar Anwar Anwar Arie Febretrisiana Arman Sayuti Arman Sayuti Arman Sayuti Arman Sayuti Arman Sayuti Arman Sayuti Ayu Wannisa Azim, Muhammad Bengi, Nanda Simah Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan budianto panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Cantika Tamara Listin Christopher R. Stremme Cut Nila Thasmi Cut Nila Thasmi Cut Nila Thasmi Cut Nila Thasmi Cut Nila Thasmi, Cut Nila Daffa Gustia Putra Akbar Dasrul Dasrul Dasrul Dasrul Dian Masyitah Dian Masyitah, Dian Dian Mulfristia Dwinna Aliza Dwinna Aliza Eka Meutia Sari Elfi Satria Suryani Erdiansyah Rahmi Evi, Siska Fadillah Fadillah Fadli A. Gani Fahrul Brinaldi Feby Claudia Sirait Fitriani Fitriani Gani, Fadli A. Gholib Gholib Gholib Gholib, Gholib Ginta Riady H Alief Hamdan Hamdan Hamdan Hamdan Hamny Sofyan Hendra Saputra Herrialfian . Herrialfian Herrialfian Herrialfian, Herrialfian Husnur rizal Husnur rizal Husnurrizal . Husnurrizal Husnurrizal Husnurrizal Husnurrizal Husnurrizal Husnurrizal Husnurrizal Husnurrizal Husnurrizal, Husnurrizal Indah Kesuma Siregar Indi Rafika Iwan Hasri Juli Melia Juli Melia Juli Melia KARTINI ERIANI Ketut Adnyane Mudite L. Praharani Lisa Praharani M Adam M Agus Setiadi M Hamdan M Jalaluddin M Syafruddin M. A. Setiadi M. Hasan M. Yusuf Mahdi Abrar Marbun, Agnes Silvina Meutia Handayani Mira Ayu Lestari Hasibuan Mirranda Fallatanza Mudhita Zikkrullah Ritonga Muhammad Bahi Muhammad Bahi Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muhammad Reza Muharriza Nasution Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Muslim Akmal Muslim Akmal Muslim Akmal Muslim Akmal N. Nazaruddin N. W. K. Karja Nabilah Putroe Agung Nazaruddin Nazaruddin Nazaruddin Nazaruddin Nellita Meutia Nellita Meutia, Nellita Ni Wayan Kurniani Karja Nurhazimah Nurhazimah Nurliana NURLIANA NURLIANA NURLIANA NURLIANA Nuzul Asmilia Nuzul Asmilia Praharani, Lisa Qathrinnada Ramadhana R Roslizawaty Razali Daud Rian Ferdiyan Ridwan Ridwan Rika Marwati Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rossa Rika Wahyuni Rumi Sahara Zamzami S Syafrudddin Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Suryani, Elfi Satria Syafruddin Syafruddin Syafruddin Syafruddin Syamsiar, Syamsiar T. Armansyah T. Armansyah TR Telambanua, Swasti Teuku Armansyah Teuku Armansyah Teuku Armansyah Teuku Armansyah Titit, Marcelus Ferdinandez Tongku N Siregar Tongku N Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar, MP Wahyuni, Sri Wenny Novita Sari Zamzami, Rumi Sahara