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Molecular characterization of Plant Growth Promoting Rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia SLAMET SANTOSA; SUTARNO SUTARNO; EDI PURWANTO; SURANTO SURANTO; SAJIDAN SAJIDAN
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190623

Abstract

Santosa S, Sutarno, Purwanto E, Suranto, Sajidan. 2018. Molecular characterization of Plant Growth Promoting Rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia. Biodiversitas 19: 2157-2162. Plant Growth Promoting Rhizobacteria (PGPR) are rhizosphere bacteria that can be utilized to increase plant growth and suppress plant diseases. PGPR concentrate on the concept of sustainable agriculture due to their role as biostimulant by synthesizing and regulating the concentration of various phytohormones and as biofertilizers. It is important to determine the original PGPR found in the roots of rice plants. This study aimed to identify PGPR based on 16S rRNA sequences. The bacterial strain was isolated from the rhizospheric soil of IR 64 organic and inorganic rice fields in the region of Sragen District, Central Java, Indonesia. The bacterial isolates were grown on the Luria Bertani (LB) Agar Medium. There were 10 colonies obtained with different morphological variations, nine of those were from the organic rice field. Molecular characterization using 16R rRNA sequences suggested the identity the nine isolates were of Pseudomonas aeruginosa strain RI-98-1, Stenotrophomonas maltophilia strain S431, Bacillus subtilis strain CEB2, Bacillus cereus strain ATCC 14579 clone EA195, S. maltophilia strain 5517, Exiguobacterium acetylicum strain SSA-3, Serratia nematodiphila strain HC4, Bacillus cereus strain ANP221, and Acinetobacter junii strain M. pstv. 21.4 Pseudomonas and Bassilus produce phytohormones (auxins) and convert inorganic phosphate to organic and Stenotropomonas can increase the growth of Acinetobacter and Exigobacterium as phosphate solvents in the soil.
Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia Suranto Suranto; DWI WAHYUNI; EDY PURWANTO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 9 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200912

Abstract

Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars and variations in the morphological characters not only for the leaves but also for the tubers. Sweet potatoes had economically significant role, especially as an additional food due to its high content of carbohydrate and other nutrient values. In order to test whether the morphological variation in this species was also expressed at the micro character; such as pollen morphology and enzyme activity, ten sweet potato cultivars were used in this study. The morphological characters such as shape and structure of the pollen features were examined using Scanning Electron Microscopy (SEM). Accordingly, Polyacrylamide Gel Electrophoresis (PAGE) was used to look-at the banding pattern of peroxidase isozymes. Morphological characters of leaves, flowers, and tuber were observed qualitatively both for the typical shape and colors. More detail of flower shapes was drown manually especially their petal edges. The results showed that there was variation among the ten sweet potato cultivars confirmed not only on their morphological characters but also the isozyme banding patterns of ten cultivars tested. At least, two cultivar Antin 3 and Papua Solossa had uniques characters especially the purple stem and yellow tuber and dark skin on the tuber, respectively. In addition, the structural analysis of pollen showed that Beta 1 cultivar has very large pollen; 116,50 ?m (axis polar) and the contour surface of exine pollen was concave with the aperture shape and ornamentation were prolate spheroidal and echinate respectively. This unique character was also shown by its peroxidase isozyme bands. Eleven bands of leaf and 13 bands of stem samples were detected respectively. The typical Rf values of 0.31, 0.48 and 0.41 were detected for the leaf, while for stem samples were 0.14, 0.20 and 0.26. The Beta 1 cultivar showed very thick bands in between Rf values of 0.13 and 0.26. Meanwhile, the unique band pattern of Papua Salosa was the absence of band at 0.38.
Short communication Phenol contaminant of Bengawan Solo River and characteristics of Portulaca using flavonoid, saponin, and tannin for phytoremediation purposes SITI KHOIRIYAH; EVI GRAVITIANI; PRABANG SETYONO; Suranto Suranto
Biodiversitas Journal of Biological Diversity Vol. 20 No. 11 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201120

Abstract

Abstract. Khoiriyah S, Gravitiani E, Setyono P, Suranto. 2019. Short communication Phenol contaminant of Bengawan Solo River and characteristics of Portulaca using flavonoid, saponin, and tannin for phytoremediation purposes. Biodiversitas 20: 3269-3274. Bengawan Solo River (BSR) as one of the largest river in Java has been contaminated by several waste products including industrial activities and organic materials produced by households along the edge of river. One of the very dangerous substances was Endocrine Disrupting Compound (EDC). This estrogenic waste solution has been considered to have a negative impact on the environmental conditions which could cause quality water becoming worse. The aims of this research were to identify the quality BSR whether or not contaminated by phenol and to reduce the very potential pollutants of EDC by introducing local native plants of Portulaca species as a candidate of phytoremediation plant agents. To characterize the three species of Portulaca, i.e. P. oleracea, P glandiflora, and P. pillosa, TLC-test to detect their flavonoid, saponin, and tannin were conducted respectively. Accordingly, HPLC was used to detect the presence of EDC. The results showed that BSR’s EDC has been detected with a concentration of 0,0013 ng. Also, all three species of Portulaca contained flavonoid, tannin, and saponin, in which the highest concentration of saponin has been detected in P. pillosa. Based on these results, it is expected that plants containing the highest secondary metabolism, such as saponin would be very promising to be used for phytoremediation purposes. The preliminary result of HPLC showed supporting this prediction.
Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia RESTYKANIA; suratman; ARI PITOYO; SURANTO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201035

Abstract

Abstract. Restykania, Suratman, Pitoyo A, Suranto. 2019. Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 20: 3024-3032. The genetic diversity among madeira vine accessions from southeastern part of Central Java has not been investigated in detail. Information on genetic diversity and relationship among accessions of this plant will provide important input into determining resourceful management strategies and guiding the improvement of plants through plant breeding programs. The objective of this study was to evaluate variation of morphology and isozyme among madeira vine (Anredera cordifolia (Ten.) Steenis) accessions from southeastern part of Central Java. A total of 13 madeira vine accessions were used for morphological observations and isozyme analysis. Morphological diversity was characterized based on measurements and observations of vegetative structures such as stems, leaves, and aerial tubers. Identification of biochemical markers was done using peroxidase and esterase isozyme systems. A relationship dendrogram among accessions was constructed based on the genetic similarity matrix by applying a cluster analysis. The analysis of quantitative and qualitative morphological characters revealed that the majority of the tested traits showed variation, indicating that there was variability among the tested madeira vine accessions. Polymorphism was observed using isozyme of peroxidase (i.e. two banding patterns) and esterase (i.e. five banding patterns). Based on the dendrogram at a level of 63 % similarity, it showed distinct separation of 13 madeira vine accessions into two major clusters. Cluster I consisted of nine accessions and the closest relationship was shown between KLT 3 and KLT 1 accessions that had 94 % of similarity coefficient. The remaining four accessions were clustered separately as Cluster II with similarity coefficient of 73 %. Therefore, genetic characterization based on morphological and isozyme markers obtained in this study is valuable to understand genetic variability and relationship among madeira vine accessions in southeastern part of Central Java.
Genetic variation of Austropuccinia psidii in some species of Myrtaceae as host plants in Java, Indonesia based on simple sequence repeats (SSR) markers FARAH AULYA FARADILLA; ISTIANA PRIHATINI; SURANTO SURANTO; ARI SUSILOWATI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230131

Abstract

Abstract. Faradilla FA, Prihatini I, Suranto, Susilowati A. 2022. Genetic variation of Austropuccinia psidii in some species of Myrtaceae as host plants in Java, Indonesia based on simple sequence repeats (SSR) markers. Biodiversitas 23: 256-263. Austropuccinia psidii is pathogenic rust with a wide host and is considered a biosecurity threat to the Myrtaceae family in many countries. The genetic variation of this rust in Java, Indonesia is poorly reported. Therefore, this study aimed to determine the genetic variation among A. psidii from different Myrtaceae hosts, namely Syzygium polyanthum, S. myrtifolium, and Melaleuca cajuputi from three different locations in Java using seven simple sequence repeats (SSR) markers. Data were collected from 28 A. psidiisamples from three different hosts and locations in Java. The genetic variations of A. psidii were found in six isolates in locus USYD_Pp168, PpSSR161, and PpSSR195*. The results showed that the expected heterozygosity value among 12 isolates of A. psidii is 0.312. The dendrogram illustrates two clusters constructed with cluster I consisting of subcluster IA (S1, S3, S4, S5, K1, K2, K3, K4, and P1) and IB (P2); and cluster II consists of isolates S2 and S6. Principal Coordinate Analysis (PCoA) was used to demonstrate the similarity and dissimilarity among isolates based on microsatellite sites. Axis 1 and 2 explained 87.7 % of the total variations and sep a rated isolate S1, S2, S3, S4, S6 from other isolates, and grouping the rest of isolates together. The dendrogram and PCo analysis demonstrated A. psidii isolates tend to have close genetic similarity based on their host.
Chlorogenic acid and caffeine content of fermented robusta bean TJAHJADI PURWOKO; SURANTO SURANTO; RATNA SETYANINGSIH; SOERYA DEWI MARLIYANA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230231

Abstract

Abstract. Purwoko T, Suranto, Setyaningsih R, Marliyana SD. 2021. Chlorogenic acid and caffeine content of fermented robusta bean. Biodiversitas 23: 902-906. Robusta beans contain caffeine and chlorogenic acid higher than arabica beans, however, it has a lower economic value than arabica beans. Therefore, microorganisms in the robusta bean for the fermentation process are expected to reduce caffeine and chlorogenic acid contents. The objective of this research was to investigate the ability of fungus Rhizopus oryzae, yeast Saccharomyces cerevisiae, and bacteria Lactobacillus casei and Leuconostoc mesenteroides in degrading caffeine and chlorogenic acid content of robusta beans. Analysis of caffeine and chlorogenic acid content was carried out by spectrophotometer at OD275 and OD324, respectively. The caffeine and chlorogenic acid content of unfermented robusta beans were 18.64 mg/g and 62.18 mg/g. Saccharomyces cerevisiae, L. mesenteroides, L. casei, and R. oryzae were able to degrade caffeine, and reduce the caffeine content of fermented robusta beans to 16.92, 16.71, 16.67, and 13.57 mg/g, respectively. The decrease of caffeine by S. cerevisiae, L. mesenteroides and L. casei were not significantly different (p>0.05) from control, however, the decrease of caffeine by R. oryzae was significant (p<0.05). Saccharomyces cerevisiae, R. oryzae, L. mesenteroide, and L. casei were able to degrade chlorogenic acid and reduce the chlorogenic acid content of fermented robusta beans to 44.54, 45.21, 45.79, and 47.31 mg/g. Chlorogenic acid was reduced significantly by S. cerevisiae, R. oryzae, L. mesenteroides, and L. casei (p<0.05). It can be concluded that R. oryzae was a potential microorganism to reduce caffeine and chlorogenic acid contents in robusta beans.
Population study of Cucurbita moschata based on morphological characters and isozyme banding patterns in Bima District, West Nusa Tenggara, Indonesia MUHAMMAD FURQAN; SURANTO SURANTO; SUGIYARTO SUGIYARTO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250104

Abstract

Abstract. Furqan M, Suranto, Sugiyarto. 2023. Population study of Cucurbita moschata based on morphological characters and isozyme banding patterns in Bima District, West Nusa Tenggara, Indonesia. Biodiversitas 25: 30-38. Pumpkin is a tropical plant that benefits from its lipid, protein, carbohydrate, and mineral contents. The morphological characters of pumpkins can be influenced by their genetics and environment. This research aimed to examine the relationship among 9 accessions of Cucurbita moschata Duchesne from 9 locations in Bima district, West Nusa Tenggara (NTB) province, Indonesia. The morphological characters of stems, leaves, and flowers were analyzed qualitatively and quantitatively and an ANOVA was used to look at the difference of leaf and flower characters. Polyacrylamide gel electrophoresis (PAGE) with peroxidase and esterase was used to detect isozyme banding patterns. Morphological characters and isozyme data were analyzed using a UPGMA method with NTSYS version 2.0 software to construct a dendrogram. The results show evidence of the varied morphological appearance of C. moschata from the different locations. A total of 12 esterase bands and 10 peroxidase bands were detected from Wera2. An undetected band with Rf value of 0.567 was recorded, while a unique band with Rf value of 0.133 (peroxidase) was not detected. Undetected bands with Rf values of 0.645 and 0.726 of esterase were also recorded.  A dendrogram showed three clusters of 9 accessions studied, in which Wera2 and Wera3 each stood alone, and a third large group was formed from the 7 remaining accessions. These results confirm the uniqueness of the Wera2 and Wera3 accessions. These results would be good information in determining a management strategy for improving the quality of pumpkins in the future.
Flavonoid compound of Cucurbita moschata at three different altitudes SURANTO SURANTO; NUR RAHMAH HIDAYATI; MUHAMMAD FURQAN; EDWI MAHADJOENO; SAJIDAN SAJIDAN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 3 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240361

Abstract

Abstract. Suranto, Hidayati NR, Furqan M, Mahadjoeno E, Sajidan. 2023. Flavonoid compound of Cucurbita moschata at three different altitudes. Biodiversitas 24: 1853-1860. The aims of this research were to examine the presence of flavonoids, both the total and the content of quercetin and kaempferol, in Cucurbita moschata (Duchesne) Duchesne ex Poir. leaf growing at different altitudes. First, Thin Layer Chromatography (TLC) was used to detect the presence of flavonoid substances, followed by the colorimetric AlCl3 method to look at the Total Flavonoid Content (TFC). Finally, one-way ANOVA was employed to analyze quantitatively. Accordingly, High-Performance Liquid Chromatography (HPLC) method was used to test whether any member of flavonoid substances was found. The results showed that flavonoid substances of C. moschata leaf samples were detected, and the Rf value of every single altitude was not significantly different. However, the highest total flavonoid content was recorded from the middle altitude (1.369 mg QE/g); the lowest was found in the highest (0.244 QE/g). Furthermore, HPLC tests showed that retention times of quercetin at every single altitude from the lowest to the highest one were not much different. Meanwhile, kaempferol's retention time was recorded at 13.001 minutes for the lowest altitude and 12.955 and 12.965 minutes for the middle and highest altitudes, respectively. These preliminary results, especially the total flavonoid content in different altitudes, could detect various flavonoid members, such as quercetin and kaempferol, for the next project.
Leaf anatomical characters variation of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications SURATMAN SURATMAN; SURANTO SURANTO; MUZZAZINAH MUZZAZINAH; PURNOMO PURNOMO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 7 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230748

Abstract

Abstract. Suratman, Suranto, Muzzazinah, Purnomo. 2022. Leaf anatomical characters variation of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications. Biodiversitas 23: 3705-3720. The objective of this study was to evaluate leaf anatomical character variation among species of Strobilanthes s.l. from Sumatra. A total of 35 species from four genera previously recognized within the subtribe Strobilanthineae (Strobilanthes, Hemigraphis, Sericocalyx, Semnostachya) were used for leaf paradermal section observations. A total of seventeen leaf anatomical characters consisting of ten quantitative and seven qualitative characters were selected for leaf anatomical comparison. The quantitative and qualitative character analysis showed great variability in the majority of tested leaf anatomical characters. This result indicates the large diversity of leaf anatomical characters occurs among species of Strobilanthes s.l. A similarity dendrogram among species of Strobilanthes s.l. based leaf anatomical character data revealed two major clusters. The first cluster consists of eleven species with regular epidermal cell shape and straight cell wall with a similarity coefficient of 0.67. The second cluster consists of twenty four species that differed from species in the first cluster due to their irregular epidermal cell shape and undulate anticlinal cell wall with a similarity coefficient of 0.59. This study indicates that leaf anatomical characters are valuable taxonomic features in taxa delimitation at the species level. The results of this study also support earlier works through molecular studies that all members of the Strobilanthinae then accepted as Strobilanthes s.l. group which could not be easily split into smaller component genera.
Pollen morphological characters variation among species of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications SURATMAN SURATMAN; SURANTO SURANTO; MUZZAZINAH MUZZAZINAH; PURNOMO PURNOMO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230930

Abstract

Abstract. Suratman, Suranto, Muzzazinah, Purnomo. 2022. Pollen morphological characters variation among species of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications. Biodiversitas 23: 4636-4648. The objective of this study was to evaluate pollen morphological characters variation and its taxonomic value among species of Strobilanthes s.l. from Sumatra. A total of 32 species from four genera previously recognized within the subtribe Strobilanthineae were used for pollen morphology observations using Light Microscope (LM) and Scanning Electron Microscope (SEM). A total of ten pollen morphological characters (both quantitative and qualitative) were observed in this study. In general, a great variation occurs in the majority of investigated pollen morphological characters. A similarity dendrogram among species of Strobilanthes s.l. based on pollen morphological character data revealed two major clusters. Cluster I consist of 25 species that differed from species in Cluster II in having pollen type I, pollen type II and pollen type III with a similarity coefficient of 0.61. Cluster II consists of seven species and has pollen type IV, pollen type V, pollen type VI and pollen type VII with a similarity coefficient of 0.46. An identification key to the species of Strobilanthes s.l. based on pollen morphology is also established for easy discrimination of the investigated taxa. This study revealed that pollen morphology was taxonomically useful character for identification and taxa delimitation of Strobilanthes s.l., especially at the species level. The results of this study also support earlier works through molecular studies, statistical analysis, gross morphology observations and leaf anatomy studies that all segregate genera of the subtribe Strobilanthinae should be merged under a single and well-defined genus as Strobilanthes s.l.
Co-Authors A.T. SYAHIDAH Achmad Achmad Adriana Grahani Firdaussy Agung Wahyudi Agus Cahyono Agus Riwanto AHMAD DWI SETYAWAN Ahmad Kailani, Ahmad AINUN NIKMATI LAILY AKHMAD MUSTOFA Alanindra Saputra Andina Elok Puri Maharani ANGELIA ARUM PRAMETA Anggara, Reksi Anggreyani, Reysa ANI SULISTYARSI Annisa Dewi Setyowati Anshori, Mahfud ARI SUSILOWATI Ari Susilowati ARI PITOYO ARI PITOYO ARI PITOYO Ari Pitoyo ARI PITOYO Ari Pitoyo ARI SUSILOWATI Aria Dirawan Arief A. Aryanto Budhy Sulihyantoro Aton Rustandi Mulyana BAMBANG SUDARMANTO Bambang Sunarto Baskoro Adi Prayitno Bengke, Melki Bertha Wikara Bintarto, Bambang Chatarina Heny Dwi Surwati Chusnu Syarifa Diah Kusuma Darmaka, Benediktus James Widya Deniawan Tommy Candra Dermawan, Dika Dewi Sekar Kencono Dhany Efita Sari Dwi Harsono DWI HASTUTI Dwi Tiyanto DWI WAHYUNI EDI PURWANTO EDWI MAHADJOENO EDWI MAHADJOENO Edwi Mahajoeno EDY PURWANTO Eka Nada Shofa Alkhajar Erham Budi Wiranto Erlina, Yenny Herawati Yohana Heylen EVI GRAVITIANI FARAH AULYA FARADILLA Farah Aulya Faradilla Farah Aulya Faradilla Fatmahwati, Seli Fauzi, Achmad Noor Firdastin Ruthnia Yudiningrum Fitriana Nur Astuti Gatot Wibowo Handayani, Arinda HAPSARI SETIA PUTRI Helmy, Mia Ferian Hendro Gunadi Henricus Hans Setyawan Prabowo Henricus Hans Setyawan Prabowo Hidayati, Muslihah Nur Husodo, Jadmiko Anom I. L. G. Nutjahjaningsih Ida Untari Idam Ragil Widianto Atmojo Ijah Mulyani Sihotang Imran, Muhammad Ali Ina Primasari Ina Primasari Isharyanto Isharyanto ISTIANA PRIHATINI Istiana Prihatini JOKO SUTRISNO Khotimah, Ainul Khotimah Lalu Usman Ali Lardianto Budhi Lesmana, Yeffry hendra MARSUSI MARSUSI Mauro Mocerino Meti Indrowati MOHAMMAD MASYKURI Mohammad Masykuri MUHAMMAD FURQAN MUHAMMAD FURQAN Muhammad Fahmi Johan Syah Muhammad Maga Sule Muhammad RIDWAN Murni Ramli Murni Ramli Muzzazinah Muzzazinah MUZZAZINAH MUZZAZINAH MUZZAZINAH MUZZAZINAH Nagoor Gafoordeen Nita Etikawati Novita Wulandari NUR RAHMAH HIDAYATI NUR RAHMAH HIDAYATI NURLIANI BERMAWIE NURLIANI BERMAWIE Oktaviariesta Habibatus Sholikhah PRABANG SETYONO PRABANG SETYONO Pramana, Edho Purin Candra Purnama PURNOMO PURNOMO PURNOMO PURNOMO Qinyu Yu Rafidiyah, Dina Rahmadhini, RR Rahajeng Suryo RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RESTYKANIA Retno Dwi Suyanti Ria Arista Asih Riezky Maya Probosari Rinawaty Rochman Hadi Mustofa Rustopo Rustopo Sabar Narimo Sajidan SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN Salma Azzah Fitran Saputri Sanastiti, Zahwa Sari Wijayanti Sarwanto Sarwanto SEPTIANA KURNIASARI Siregar, Yani Sukriah SITI KHOIRIYAH Siti Rodhiah Azhari Slamet Santosa SLAMET SANTOSA SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA Solichatun Solichatun Solichatun Solichatun SOLIKAH ANA ESTIKOMAH Sri Handayani Sri Handayani Sri Handayani Sri Herwindya Baskara Wijaya sri herwindya baskara wijaya SRI MULYANI Sri Wahyuni SUDARMONO SUDARMONO SUGIYARTO SUGIYARTO SUGIYARTO SUGIYARTO Sukodoyo Sukodoyo Sularso Sularso Sulasmi Sulasmi Sunarto Sunarto Sunny Ummul Firdaus SUNTORO SUNTORO SUPRIYADI SUPRIYADI Supyani Supyani Supyani Supyani suratman SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN Suratman Suratman SURATMAN SURATMAN Suratman, Suratman Sutarno Sutarno Sutarno Sutarno SUTARNO SUTARNO Tanjung Ardo TIAS HESTI KUSUMAWATI TJAHJADI PURWOKO TJAHJADI PURWOKO Tri Saputra Medhācitto TRIBADI TRIBADI TUNJUNG SULAKSONO UMI FATMAWATI Umi Fatmawati Umi Fatmawati Veronika, Nofita Wahyu Pangestuning Astuti Wahyuni Purnami Wicaksono, Dimas Suryo Widya Mudyantini Widya Mudyantini WIRYANTO WIRYANTO Zakarijah, Edi Zufahmi Zufahmi Zulkarnain Mistortoify