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Genetic variation of Austropuccinia psidii in some species of Myrtaceae as host plants in Java, Indonesia based on simple sequence repeats (SSR) markers FARAH AULYA FARADILLA; ISTIANA PRIHATINI; SURANTO SURANTO; ARI SUSILOWATI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230131

Abstract

Abstract. Faradilla FA, Prihatini I, Suranto, Susilowati A. 2022. Genetic variation of Austropuccinia psidii in some species of Myrtaceae as host plants in Java, Indonesia based on simple sequence repeats (SSR) markers. Biodiversitas 23: 256-263. Austropuccinia psidii is pathogenic rust with a wide host and is considered a biosecurity threat to the Myrtaceae family in many countries. The genetic variation of this rust in Java, Indonesia is poorly reported. Therefore, this study aimed to determine the genetic variation among A. psidii from different Myrtaceae hosts, namely Syzygium polyanthum, S. myrtifolium, and Melaleuca cajuputi from three different locations in Java using seven simple sequence repeats (SSR) markers. Data were collected from 28 A. psidiisamples from three different hosts and locations in Java. The genetic variations of A. psidii were found in six isolates in locus USYD_Pp168, PpSSR161, and PpSSR195*. The results showed that the expected heterozygosity value among 12 isolates of A. psidii is 0.312. The dendrogram illustrates two clusters constructed with cluster I consisting of subcluster IA (S1, S3, S4, S5, K1, K2, K3, K4, and P1) and IB (P2); and cluster II consists of isolates S2 and S6. Principal Coordinate Analysis (PCoA) was used to demonstrate the similarity and dissimilarity among isolates based on microsatellite sites. Axis 1 and 2 explained 87.7 % of the total variations and sep a rated isolate S1, S2, S3, S4, S6 from other isolates, and grouping the rest of isolates together. The dendrogram and PCo analysis demonstrated A. psidii isolates tend to have close genetic similarity based on their host.
Chlorogenic acid and caffeine content of fermented robusta bean TJAHJADI PURWOKO; SURANTO SURANTO; RATNA SETYANINGSIH; SOERYA DEWI MARLIYANA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230231

Abstract

Abstract. Purwoko T, Suranto, Setyaningsih R, Marliyana SD. 2021. Chlorogenic acid and caffeine content of fermented robusta bean. Biodiversitas 23: 902-906. Robusta beans contain caffeine and chlorogenic acid higher than arabica beans, however, it has a lower economic value than arabica beans. Therefore, microorganisms in the robusta bean for the fermentation process are expected to reduce caffeine and chlorogenic acid contents. The objective of this research was to investigate the ability of fungus Rhizopus oryzae, yeast Saccharomyces cerevisiae, and bacteria Lactobacillus casei and Leuconostoc mesenteroides in degrading caffeine and chlorogenic acid content of robusta beans. Analysis of caffeine and chlorogenic acid content was carried out by spectrophotometer at OD275 and OD324, respectively. The caffeine and chlorogenic acid content of unfermented robusta beans were 18.64 mg/g and 62.18 mg/g. Saccharomyces cerevisiae, L. mesenteroides, L. casei, and R. oryzae were able to degrade caffeine, and reduce the caffeine content of fermented robusta beans to 16.92, 16.71, 16.67, and 13.57 mg/g, respectively. The decrease of caffeine by S. cerevisiae, L. mesenteroides and L. casei were not significantly different (p>0.05) from control, however, the decrease of caffeine by R. oryzae was significant (p<0.05). Saccharomyces cerevisiae, R. oryzae, L. mesenteroide, and L. casei were able to degrade chlorogenic acid and reduce the chlorogenic acid content of fermented robusta beans to 44.54, 45.21, 45.79, and 47.31 mg/g. Chlorogenic acid was reduced significantly by S. cerevisiae, R. oryzae, L. mesenteroides, and L. casei (p<0.05). It can be concluded that R. oryzae was a potential microorganism to reduce caffeine and chlorogenic acid contents in robusta beans.
Population study of Cucurbita moschata based on morphological characters and isozyme banding patterns in Bima District, West Nusa Tenggara, Indonesia MUHAMMAD FURQAN; SURANTO SURANTO; SUGIYARTO SUGIYARTO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250104

Abstract

Abstract. Furqan M, Suranto, Sugiyarto. 2023. Population study of Cucurbita moschata based on morphological characters and isozyme banding patterns in Bima District, West Nusa Tenggara, Indonesia. Biodiversitas 25: 30-38. Pumpkin is a tropical plant that benefits from its lipid, protein, carbohydrate, and mineral contents. The morphological characters of pumpkins can be influenced by their genetics and environment. This research aimed to examine the relationship among 9 accessions of Cucurbita moschata Duchesne from 9 locations in Bima district, West Nusa Tenggara (NTB) province, Indonesia. The morphological characters of stems, leaves, and flowers were analyzed qualitatively and quantitatively and an ANOVA was used to look at the difference of leaf and flower characters. Polyacrylamide gel electrophoresis (PAGE) with peroxidase and esterase was used to detect isozyme banding patterns. Morphological characters and isozyme data were analyzed using a UPGMA method with NTSYS version 2.0 software to construct a dendrogram. The results show evidence of the varied morphological appearance of C. moschata from the different locations. A total of 12 esterase bands and 10 peroxidase bands were detected from Wera2. An undetected band with Rf value of 0.567 was recorded, while a unique band with Rf value of 0.133 (peroxidase) was not detected. Undetected bands with Rf values of 0.645 and 0.726 of esterase were also recorded.  A dendrogram showed three clusters of 9 accessions studied, in which Wera2 and Wera3 each stood alone, and a third large group was formed from the 7 remaining accessions. These results confirm the uniqueness of the Wera2 and Wera3 accessions. These results would be good information in determining a management strategy for improving the quality of pumpkins in the future.
Flavonoid compound of Cucurbita moschata at three different altitudes SURANTO SURANTO; NUR RAHMAH HIDAYATI; MUHAMMAD FURQAN; EDWI MAHADJOENO; SAJIDAN SAJIDAN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 3 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240361

Abstract

Abstract. Suranto, Hidayati NR, Furqan M, Mahadjoeno E, Sajidan. 2023. Flavonoid compound of Cucurbita moschata at three different altitudes. Biodiversitas 24: 1853-1860. The aims of this research were to examine the presence of flavonoids, both the total and the content of quercetin and kaempferol, in Cucurbita moschata (Duchesne) Duchesne ex Poir. leaf growing at different altitudes. First, Thin Layer Chromatography (TLC) was used to detect the presence of flavonoid substances, followed by the colorimetric AlCl3 method to look at the Total Flavonoid Content (TFC). Finally, one-way ANOVA was employed to analyze quantitatively. Accordingly, High-Performance Liquid Chromatography (HPLC) method was used to test whether any member of flavonoid substances was found. The results showed that flavonoid substances of C. moschata leaf samples were detected, and the Rf value of every single altitude was not significantly different. However, the highest total flavonoid content was recorded from the middle altitude (1.369 mg QE/g); the lowest was found in the highest (0.244 QE/g). Furthermore, HPLC tests showed that retention times of quercetin at every single altitude from the lowest to the highest one were not much different. Meanwhile, kaempferol's retention time was recorded at 13.001 minutes for the lowest altitude and 12.955 and 12.965 minutes for the middle and highest altitudes, respectively. These preliminary results, especially the total flavonoid content in different altitudes, could detect various flavonoid members, such as quercetin and kaempferol, for the next project.
Leaf anatomical characters variation of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications SURATMAN SURATMAN; SURANTO SURANTO; MUZZAZINAH MUZZAZINAH; PURNOMO PURNOMO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 7 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230748

Abstract

Abstract. Suratman, Suranto, Muzzazinah, Purnomo. 2022. Leaf anatomical characters variation of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications. Biodiversitas 23: 3705-3720. The objective of this study was to evaluate leaf anatomical character variation among species of Strobilanthes s.l. from Sumatra. A total of 35 species from four genera previously recognized within the subtribe Strobilanthineae (Strobilanthes, Hemigraphis, Sericocalyx, Semnostachya) were used for leaf paradermal section observations. A total of seventeen leaf anatomical characters consisting of ten quantitative and seven qualitative characters were selected for leaf anatomical comparison. The quantitative and qualitative character analysis showed great variability in the majority of tested leaf anatomical characters. This result indicates the large diversity of leaf anatomical characters occurs among species of Strobilanthes s.l. A similarity dendrogram among species of Strobilanthes s.l. based leaf anatomical character data revealed two major clusters. The first cluster consists of eleven species with regular epidermal cell shape and straight cell wall with a similarity coefficient of 0.67. The second cluster consists of twenty four species that differed from species in the first cluster due to their irregular epidermal cell shape and undulate anticlinal cell wall with a similarity coefficient of 0.59. This study indicates that leaf anatomical characters are valuable taxonomic features in taxa delimitation at the species level. The results of this study also support earlier works through molecular studies that all members of the Strobilanthinae then accepted as Strobilanthes s.l. group which could not be easily split into smaller component genera.
Pollen morphological characters variation among species of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications SURATMAN SURATMAN; SURANTO SURANTO; MUZZAZINAH MUZZAZINAH; PURNOMO PURNOMO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230930

Abstract

Abstract. Suratman, Suranto, Muzzazinah, Purnomo. 2022. Pollen morphological characters variation among species of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications. Biodiversitas 23: 4636-4648. The objective of this study was to evaluate pollen morphological characters variation and its taxonomic value among species of Strobilanthes s.l. from Sumatra. A total of 32 species from four genera previously recognized within the subtribe Strobilanthineae were used for pollen morphology observations using Light Microscope (LM) and Scanning Electron Microscope (SEM). A total of ten pollen morphological characters (both quantitative and qualitative) were observed in this study. In general, a great variation occurs in the majority of investigated pollen morphological characters. A similarity dendrogram among species of Strobilanthes s.l. based on pollen morphological character data revealed two major clusters. Cluster I consist of 25 species that differed from species in Cluster II in having pollen type I, pollen type II and pollen type III with a similarity coefficient of 0.61. Cluster II consists of seven species and has pollen type IV, pollen type V, pollen type VI and pollen type VII with a similarity coefficient of 0.46. An identification key to the species of Strobilanthes s.l. based on pollen morphology is also established for easy discrimination of the investigated taxa. This study revealed that pollen morphology was taxonomically useful character for identification and taxa delimitation of Strobilanthes s.l., especially at the species level. The results of this study also support earlier works through molecular studies, statistical analysis, gross morphology observations and leaf anatomy studies that all segregate genera of the subtribe Strobilanthinae should be merged under a single and well-defined genus as Strobilanthes s.l.
Measuring climate action readiness in maintaining ecological resilience using satellite imagery and field research in Garang Watershed, Central Java, Indonesia BAMBANG SUDARMANTO; SURANTO SURANTO; SUNTORO SUNTORO; JOKO SUTRISNO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240551

Abstract

Abstract. Sudarmanto B, Suranto S, Suntoro S, Sutrisno J. 2023. Measuring climate action readiness in maintaining ecological resilience using satellite imagery and field research in Garang Watershed, Central Java, Indonesia. Biodiversitas 24: 2958-2974. The long-term viability of vegetation as a representation of land cover in a watershed is critical. However, more work is needed to develop vegetation management in the form of a climate action model that considers the existence of ecological and social values as a unified system, particularly in times of climate change. Moran's Index measurements and statistical correlation analysis were employed in this study to quantify geographical patterns. These measurements provide real-world judgment in developing aggressive climate response scenarios. Ecological values were derived from shifting vegetation trend indices due to mutual interaction between living beings that build a life cycle in harmony with the environment. Meanwhile, social values are determined by assessing individual internal factors such as attitudes, knowledge, and responses and external factors represented by local government institutions. The findings reveal that the distribution of residents and residential areas is dispersed. With a high confidence level, a linear index declines with R2 = 0.5872 for inhabitants and R2 = 0.9171 for residential areas. In the dry season, there is a significant relationship between the spatial pattern of vegetation indices and the inhabitant's index. The community's knowledge and attitudes considerably impact changes in vegetation indices, particularly during the rainy season, with R2 = 0.207 for SAVI and 0.232 for NDVI. Community readiness values, which elaborate on knowledge, attitudes, responses, and the role of external institutions, show that community readiness in the upstream area of the watershed, specifically in the Kendal District, is in the best position, with a value of 6.627976. On the contrary, the Semarang District, the upstream area, has the lowest value of 4.257092.
Caffeine degradation by food microorganisms TJAHJADI PURWOKO; SURANTO SURANTO; RATNA SETYANINGSIH; SOERYA DEWI MARLIYANA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 6 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240647

Abstract

Abstract. Purwoko T, Suranto, Setyaningsih R, Marliyana SD. 2023. Caffeine degradation by food microorganisms. Biodiversitas 24: 3495-3502. Commercial coffee beans are dominated by robusta and arabica coffee beans. Caffeine is one of the important components in coffee beans. Caffeine has antimicrobial effect. Caffeine content in robusta beans was higher than in arabica beans. Caffeine content in coffee beans was affected by the coffee species, the coffee cultivation's altitude and the postharvest processing method. Microbial fermentation activity could reduce the caffeine content of coffee beans. Lactobacillus casei, Leuconostoc mesenteroides, Rhizopus oryzae and Saccharomyces cerevisiae, were able to reduce caffeine content of robusta beans. This study aimed to determine the degradation pathway of caffeine by food microorganisms, namely L. casei, L. mesenteroides, R. oryzae and S. cerevisiae. Caffeine content in NB-caffeine and PDB-caffeine media were reduced by L. casei, L. mesenteroides, R. oryzae and S. cerevisiae. Caffeine was transformed into dimethylxanthine and then into methylxanthine by L. casei, L. mesenteroides, R. oryzae and S. cerevisiae. They transformed more than 89% caffeine into paraxanthine, however, small amount of paraxanthine was transformed into methylxanthine. L. casei and L. mesenteroides transformed paraxanthine into 1-methylxanthine. However, R. oryzae and S. cerevisiae transformed into 7-methylxanthine. There were two patterns of degradation of caffeine into methylxanthine i.e., caffeine-paraxanthine-1-methylxanthine and caffeine-paraxanthine-7-methylxanthine. The first was shown by L. casei and L. mesenteroides, and the last by R. oryzae and S. cerevisiae.
Bacterial diversity in cheese wastewater using Next-Generation Sequencing (NGS) SOLIKAH ANA ESTIKOMAH; SURANTO SURANTO; ARI SUSILOWATI; MOHAMMAD MASYKURI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 2 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250206

Abstract

Abstract. Estikomah SA, Suranto, Susilowati A, Masykuri M. 2024. Bacterial diversity in cheese wastewater using Next-Generation Sequencing (NGS). Biodiversitas 25: 482-490. Cheese wastewater (whey) has a high content of organic substances, including lactose, protein, and fat. The lactose in whey wastewater can also be used as a bacterial growth medium. Bacterial community structure is an essential aspect of microbial water quality. The bacterial diversity data obtained can then be used to evaluate the existence of bacteria that might be useful for making microbiological products. This research employs Next Generation Sequencing (NGS) technology to determine the diversity and abundance of bacteria based on 16S rRNA gene amplicons for further processing of whey wastewater. The NGS-based technique overcomes the limitations of conventional bacterial culture techniques. The effectiveness and accuracy of microbial diversity analysis employing NGS technology are high. The research method includes the steps of sample preparation, DNA extraction using a ZymoBIOMICS DNA Microprep Kit (D4300), PCR amplification of the V3-V4 16S rRNA gene region, DNA sequencing, and a bioinformatics-statistical analysis. The results show that bacterial diversity in whey wastewater was found to have an average number of Operational Taxon Units (OTUs) of 259 tags. The metagenomics study of the microbial community in whey wastewater successfully detected the dominant genus of bacteria, which can benefit the management of whey wastewater. The presence of Lactobacillus and Acetobacter confirms that cheese whey wastewater exists in Yogyakarta Province. Acetobacter can oxidize ethanol to produce acetic acid, a pungent odor characteristic that causes environmental pollution. On the other hand, the presence of Lactobacillus and Acetobacter shows that whey wastewater can be reprocessed to produce fermented beverages, thereby improving their value and minimizing the impact of environmental pollution.
Short Communication: Variation in isozymic pattern of germplasm from three ginger (Zingiber officinale) varieties AHMAD DWI SETYAWAN; WIRYANTO WIRYANTO; SURANTO SURANTO; NURLIANI BERMAWIE
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060113

Abstract

Abstract. Setyawan AD, Wiryanto, Suranto, Bermawie N. 2014. Variation in isozymic pattern of germplasm from three of ginger (Zingiber officinale) varieties. Nusantara Bioscience 6: 86-93. Ginger (Zingiber officinale Rosc.) has long been as spices, flavoring agent and raw material for herbal medicines. In Indonesia, there were three varieties based on color and size of the rhizome, i.e. gajah (big-white ginger), merah (red ginger), and emprit (small-white ginger). This research was conducted to find out: (i) isozymic pattern of three ginger varieties, and (ii) phylogenetic relationship of those three varieties. The plant materials were gathered from Wonogiri, Surakarta and Kulonprogo, Yogyakarta. Two enzyme systems, namely esterase (EST) and peroxidase (PER, PRX) were used in this study. The relationship among ginger varieties was determined by UPGMA. The result indicated that EST showed two bands (i.e. Rf 0.04 and 0.10), and PRX showed six bands (i.e. Rf 0.04, 0.05, 0.09, 0.10, 0.11, and 0.15). Peroxidase produces more numerous and more diverse isozymic bands than esterase, resulting in a more complex relationship. The data used to compile dendrogram affect the grouping; the more data used, the more obvious clustering of accessions in a population. Dendrogam generated from esterase and peroxidase banding patterns produced distinct clusters based on varieties and location.
Co-Authors A.T. SYAHIDAH Achmad Achmad Adimas Maditra Permana Adriana Grahani Firdaussy Agung Wahyudi Agus Riwanto Ahimsa Adi Wibowo AHMAD DWI SETYAWAN Ahmad Kailani, Ahmad AINUN NIKMATI LAILY Akhmad Mustofa Alanindra Saputra Andika Rizky Febrianto Andina Elok Puri Maharani ANGELIA ARUM PRAMETA Anggara, Reksi Anggreyani, Reysa ANI SULISTYARSI Annisa Dewi Setyowati Ari Susilowati ARI SUSILOWATI Ari Pitoyo ARI PITOYO Ari Pitoyo ARI PITOYO ARI PITOYO ARI PITOYO Ari Pitoyo ARI SUSILOWATI Aria Dirawan Arief A. Aryanto Budhy Sulihyantoro BAMBANG SUDARMANTO Bambang Sunarto Baskoro Adi Prayitno Bengke, Melki Bertha Wikara Bintarto, Bambang Chusnu Syarifa Diah Kusuma Darmaka, Benediktus James Widya Deby Fitriana Deniawan Tommy Candra Deniawan Tommy Chandra Wijaya Dermawan, Dika Dewi Sekar Kencono Dhany Efita Sari Dina Selia Wardani Dwi Harsono DWI HASTUTI Dwi Tiyanto DWI WAHYUNI EDI PURWANTO EDWI MAHADJOENO EDWI MAHADJOENO Edwi Mahajoeno EDY PURWANTO Eka Nada Shofa Alkhajar Erham Budi Wiranto Erlina, Yenny Herawati Yohana Heylen Evi Gravitiani FARAH AULYA FARADILLA Farah Aulya Faradilla Farah Aulya Faradilla Fatmahwati, Seli Fauzi, Achmad Noor Fitriana Nur Astuti Gatot Wibowo Handayani, Arinda Hanifah Islamiyah HAPSARI SETIA PUTRI Helmy, Mia Ferian Hendro Gunadi Henricus Hans Setyawan Prabowo Henricus Hans Setyawan Wibowo Hidayati, Muslihah Nur Husna nur Amalina Husodo, Jadmiko Anom I. L. G. Nutjahjaningsih Ida Untari Idam Ragil Widianto Atmojo Iim Fathimah Ijah Mulyani Sihotang Imran, Muhammad Ali Ina Primasari Ina Primasari Irianti, Indah Susi Isharyanto Isharyanto ISTIANA PRIHATINI Istiana Prihatini Joko Sutrisno Khotimah, Ainul Khotimah KRT Nur Suhascaryo Lalu Usman Ali Lesmana, Yeffry hendra MARSUSI MARSUSI Mauro Mocerino Meti Indrowati MOHAMMAD MASYKURI Mohammad Masykuri MUHAMMAD FURQAN MUHAMMAD FURQAN Muhammad Fahmi Johan Syah Muhammad Maga Sule Muhammad Nauval Rizki Muhammad RIDWAN Murni Ramli Murni Ramli MUZZAZINAH MUZZAZINAH MUZZAZINAH MUZZAZINAH Muzzazinah Muzzazinah Nagoor Gafoordeen Nita Etikawati Novita Wulandari NUR RAHMAH HIDAYATI NUR RAHMAH HIDAYATI NURLIANI BERMAWIE NURLIANI BERMAWIE Oktaviariesta Habibatus Sholikhah PRABANG SETYONO PRABANG SETYONO Pramana, Edho Purin Candra Purnama Purin Candra Purnama PURNOMO PURNOMO PURNOMO PURNOMO Rafidiyah, Dina Rahmadhini, RR Rahajeng Suryo RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RESTYKANIA Retno Dwi Suyanti Ria Arista Asih Riezky Maya Probosari Riko Meidiya Putra Rinawaty Rochman Hadi Mustofa Rustopo Rustopo Sabar Narimo Sajidan SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN Salma Azzah Fitran Saputri Sanastiti, Zahwa Sari Wijayanti Sarwanto Sarwanto SEPTIANA KURNIASARI Siregar, Yani Sukriah SITI KHOIRIYAH Siti Rodhiah Azhari Slamet Kurniansyah Slamet Santosa SLAMET SANTOSA SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA Solichatun Solichatun Solichatun Solichatun Solichatun Solichatun SOLIKAH ANA ESTIKOMAH Sri Handayani Sri Handayani Sri Handayani sri herwindya baskara wijaya Sri Herwindya Baskara Wijaya SRI MULYANI Sri Wahyuni SUDARMONO SUDARMONO SUGIYARTO SUGIYARTO SUGIYARTO SUGIYARTO Sukodoyo Sukodoyo Sulasmi Sulasmi Sumardi Sunarto Sunarto Sunny Ummul Firdaus SUNTORO SUNTORO SUPRIYADI SUPRIYADI Supyani Supyani Supyani Supyani suratman SURATMAN SURATMAN SURATMAN SURATMAN Suratman Suratman SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN Suratman, Suratman Sutarno Sutarno SUTARNO SUTARNO Sutarno Sutarno Tanjung Ardo TIAS HESTI KUSUMAWATI TJAHJADI PURWOKO TJAHJADI PURWOKO TJAHJADI PURWOKO Tri Saputra Medhācitto TRIBADI TRIBADI TUNJUNG SULAKSONO Umi Fatmawati UMI FATMAWATI Umi Fatmawati Veronika, Nofita Vionita Dyah Pramesthi Wahyu Haspri Nur Taryanti Wahyu Pangestuning Astuti Wahyuni Purnami Wicaksono, Dimas Suryo Widya Mudyantini Widya Mudyantini Widya Mudyantini WIRYANTO WIRYANTO Zakarijah, Edi Zufahmi Zufahmi