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Measuring climate action readiness in maintaining ecological resilience using satellite imagery and field research in Garang Watershed, Central Java, Indonesia BAMBANG SUDARMANTO; SURANTO SURANTO; SUNTORO SUNTORO; JOKO SUTRISNO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240551

Abstract

Abstract. Sudarmanto B, Suranto S, Suntoro S, Sutrisno J. 2023. Measuring climate action readiness in maintaining ecological resilience using satellite imagery and field research in Garang Watershed, Central Java, Indonesia. Biodiversitas 24: 2958-2974. The long-term viability of vegetation as a representation of land cover in a watershed is critical. However, more work is needed to develop vegetation management in the form of a climate action model that considers the existence of ecological and social values as a unified system, particularly in times of climate change. Moran's Index measurements and statistical correlation analysis were employed in this study to quantify geographical patterns. These measurements provide real-world judgment in developing aggressive climate response scenarios. Ecological values were derived from shifting vegetation trend indices due to mutual interaction between living beings that build a life cycle in harmony with the environment. Meanwhile, social values are determined by assessing individual internal factors such as attitudes, knowledge, and responses and external factors represented by local government institutions. The findings reveal that the distribution of residents and residential areas is dispersed. With a high confidence level, a linear index declines with R2 = 0.5872 for inhabitants and R2 = 0.9171 for residential areas. In the dry season, there is a significant relationship between the spatial pattern of vegetation indices and the inhabitant's index. The community's knowledge and attitudes considerably impact changes in vegetation indices, particularly during the rainy season, with R2 = 0.207 for SAVI and 0.232 for NDVI. Community readiness values, which elaborate on knowledge, attitudes, responses, and the role of external institutions, show that community readiness in the upstream area of the watershed, specifically in the Kendal District, is in the best position, with a value of 6.627976. On the contrary, the Semarang District, the upstream area, has the lowest value of 4.257092.
Caffeine degradation by food microorganisms TJAHJADI PURWOKO; SURANTO SURANTO; RATNA SETYANINGSIH; SOERYA DEWI MARLIYANA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 6 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240647

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Abstract. Purwoko T, Suranto, Setyaningsih R, Marliyana SD. 2023. Caffeine degradation by food microorganisms. Biodiversitas 24: 3495-3502. Commercial coffee beans are dominated by robusta and arabica coffee beans. Caffeine is one of the important components in coffee beans. Caffeine has antimicrobial effect. Caffeine content in robusta beans was higher than in arabica beans. Caffeine content in coffee beans was affected by the coffee species, the coffee cultivation's altitude and the postharvest processing method. Microbial fermentation activity could reduce the caffeine content of coffee beans. Lactobacillus casei, Leuconostoc mesenteroides, Rhizopus oryzae and Saccharomyces cerevisiae, were able to reduce caffeine content of robusta beans. This study aimed to determine the degradation pathway of caffeine by food microorganisms, namely L. casei, L. mesenteroides, R. oryzae and S. cerevisiae. Caffeine content in NB-caffeine and PDB-caffeine media were reduced by L. casei, L. mesenteroides, R. oryzae and S. cerevisiae. Caffeine was transformed into dimethylxanthine and then into methylxanthine by L. casei, L. mesenteroides, R. oryzae and S. cerevisiae. They transformed more than 89% caffeine into paraxanthine, however, small amount of paraxanthine was transformed into methylxanthine. L. casei and L. mesenteroides transformed paraxanthine into 1-methylxanthine. However, R. oryzae and S. cerevisiae transformed into 7-methylxanthine. There were two patterns of degradation of caffeine into methylxanthine i.e., caffeine-paraxanthine-1-methylxanthine and caffeine-paraxanthine-7-methylxanthine. The first was shown by L. casei and L. mesenteroides, and the last by R. oryzae and S. cerevisiae.
Bacterial diversity in cheese wastewater using Next-Generation Sequencing (NGS) SOLIKAH ANA ESTIKOMAH; SURANTO SURANTO; ARI SUSILOWATI; MOHAMMAD MASYKURI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 2 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250206

Abstract

Abstract. Estikomah SA, Suranto, Susilowati A, Masykuri M. 2024. Bacterial diversity in cheese wastewater using Next-Generation Sequencing (NGS). Biodiversitas 25: 482-490. Cheese wastewater (whey) has a high content of organic substances, including lactose, protein, and fat. The lactose in whey wastewater can also be used as a bacterial growth medium. Bacterial community structure is an essential aspect of microbial water quality. The bacterial diversity data obtained can then be used to evaluate the existence of bacteria that might be useful for making microbiological products. This research employs Next Generation Sequencing (NGS) technology to determine the diversity and abundance of bacteria based on 16S rRNA gene amplicons for further processing of whey wastewater. The NGS-based technique overcomes the limitations of conventional bacterial culture techniques. The effectiveness and accuracy of microbial diversity analysis employing NGS technology are high. The research method includes the steps of sample preparation, DNA extraction using a ZymoBIOMICS DNA Microprep Kit (D4300), PCR amplification of the V3-V4 16S rRNA gene region, DNA sequencing, and a bioinformatics-statistical analysis. The results show that bacterial diversity in whey wastewater was found to have an average number of Operational Taxon Units (OTUs) of 259 tags. The metagenomics study of the microbial community in whey wastewater successfully detected the dominant genus of bacteria, which can benefit the management of whey wastewater. The presence of Lactobacillus and Acetobacter confirms that cheese whey wastewater exists in Yogyakarta Province. Acetobacter can oxidize ethanol to produce acetic acid, a pungent odor characteristic that causes environmental pollution. On the other hand, the presence of Lactobacillus and Acetobacter shows that whey wastewater can be reprocessed to produce fermented beverages, thereby improving their value and minimizing the impact of environmental pollution.
Short Communication: Variation in isozymic pattern of germplasm from three ginger (Zingiber officinale) varieties AHMAD DWI SETYAWAN; WIRYANTO WIRYANTO; SURANTO SURANTO; NURLIANI BERMAWIE
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060113

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Abstract. Setyawan AD, Wiryanto, Suranto, Bermawie N. 2014. Variation in isozymic pattern of germplasm from three of ginger (Zingiber officinale) varieties. Nusantara Bioscience 6: 86-93. Ginger (Zingiber officinale Rosc.) has long been as spices, flavoring agent and raw material for herbal medicines. In Indonesia, there were three varieties based on color and size of the rhizome, i.e. gajah (big-white ginger), merah (red ginger), and emprit (small-white ginger). This research was conducted to find out: (i) isozymic pattern of three ginger varieties, and (ii) phylogenetic relationship of those three varieties. The plant materials were gathered from Wonogiri, Surakarta and Kulonprogo, Yogyakarta. Two enzyme systems, namely esterase (EST) and peroxidase (PER, PRX) were used in this study. The relationship among ginger varieties was determined by UPGMA. The result indicated that EST showed two bands (i.e. Rf 0.04 and 0.10), and PRX showed six bands (i.e. Rf 0.04, 0.05, 0.09, 0.10, 0.11, and 0.15). Peroxidase produces more numerous and more diverse isozymic bands than esterase, resulting in a more complex relationship. The data used to compile dendrogram affect the grouping; the more data used, the more obvious clustering of accessions in a population. Dendrogam generated from esterase and peroxidase banding patterns produced distinct clusters based on varieties and location.
Short Communication:Comparisons of isozyme diversity in local Java cardamom (Amomum compactum) and true cardamom (Elettaria cardamomum) AHMAD DWI SETYAWAN; WIRYANTO WIRYANTO; SURANTO SURANTO; NURLIANI BERMAWIE; SUDARMONO SUDARMONO
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060114

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Setyawan AD, Wiryanto,Suranto, Bermawie N, Sudarmono. 2014. Comparisons of isozyme diversity in local Java cardamom (Amomum compactum) and true cardamom (Elettaria cardamomum). Nusantara Bioscience 6: 94-101.Fruits of Java cardamoms (Amomum compactum) and true cardamoms (Elettaria cardamomum) had long been used as spices, flavoring agent, garnishing plants etc. This research was conducted to find out: (i) variation of isozymic bands in some population of Java cardamoms and true cardamoms; and (ii) phylogenetic relationship of these cardamoms based on variation of isozymic bands. Plant material (i.e. rhizome) of Java cardamoms was collected from Bogor Botanical Garden, and plant material of true cardamoms was gathered from Indonesian Medicinal and Aromatic Crops Research Institute, Bogor, Indonesia. Ten accessions were assayed in every population. The two isozymic systems was assayed, namely esterase (EST) and peroxidase (PER, PRX). Phylogenetic relationship was determined by UPGMA method. The results showed that esterase gave nine isozymic bands, i.e. Rf 0.15, 0.26, 0.29, 0.33, 0.38, 0.42, 0.45, 0.53, and Rf 0.58., while peroxidase gave 10 isozymic bands, i.e. Rf 0.06, 0.14, 0.18, 0.22, 0.26, 0.29, 0.32, 0.37, 0.41, and Rf 0.46. Relationship dendrogram indicated that the number of data will affect the grouping based on species similarity; and more data was increasingly apparent in groupings; within these groups there were variations among its members.
The total protein band profile of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus ANI SULISTYARSI; SURANTO SURANTO; SUPRIYADI SUPRIYADI
Nusantara Bioscience Vol. 4 No. 1 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040107

Abstract

Abstract. Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Nusantara Bioscience 4: 32-35. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer mercapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108 and 117 kDa. These proteins are presumably from the group of ?-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference in the protein expression in those vectors, but slightly thicker band were observed in the infected leaves.
Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern AINUN NIKMATI LAILY; SURANTO SURANTO; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 4 No. 1 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040104

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Abstract. Laily AN, Suranto, Sugiyarto. 2012. Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern. Nusantara Bioscience 4: 16-21. Carica pubescens Lenne & K. Koch is a species of fruit plant firstly cultivated in South America and has adapted to the highland environment, such as Dieng Plateau, Central Java (±2000 m asl). It has a narrow habitat range and limited or unknown intraspecies variation. Therefore, important information about the characters of the plants at various altitudes is needed, so that it is possible to extend its distribution through transplantation to other areas. Characterization can be performed based on morphological characters, chemical content, and protein banding patterns. This study aimed to describe the morphological characters, the chemical content (antioxidant capacity), and the pattern of protein bands by staining, using Coomassie brilliant blue on C. pubescens in the Dieng Plateau. The research was conducted in the villages of Kejajar (1400 m asl), Patak Banteng (1900 m asl.), and Sembungan (2400 m asl.). The observations of morphological characters were conducted in the field and continued in the laboratory. Morphological characters, the chemical content (antioxidant capacity), and the banding pattern of protein of C. pubescens were analyzed descriptively. The results showed that the morphological characters of C. pubescens in Dieng Plateau varied in stems, leaves, and fruits. The antioxidant capacity decreased with decreasing habitat altitude, 2400 m asl. altitude> 1900 m altitude> 1400 m asl.. The Protein banding patterns did not vary, but the pattern in C. Papaya was different. The uniformity of the pattern of protein bands showed that genetic stability in C. pubescens was not affected by environmental factors.
Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java TRIBADI TRIBADI; SURANTO SURANTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020103

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Abstract. Tribadi, Suranto, Sajidan. 2009. Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java. Nusantara Bioscience 2: 14-22. This research is intended to find out the morphological and anatomical variation as well as the protein band pattern of cassava (Manihot esculenta Crantz) widely spread in three different areas of height. The sample collecting is done using simple random sampling in the three different areas of height that is 50, 300, 1000 meters asl in Ngawi District, East Java while the analysis of protein band pattern is done using SDS-PAGE. The result of the research of morphology and anatomy is analyzed descriptively and presented in the form of tables, histograms, and figures. The analysis of protein band pattern is done using quantitative and qualitative analysis that is based on the appearance or not the gel band pattern by counting the molecular weights based on code marker S 8445 and qualitative method based on the quality of the band formed. The band pattern formed is istimated and presented in the form of zimogram. The result of the research shows that the height of the cultivating site very much influences toward variations of root, stem and leaf morphology. The longest root is at 50-meter heights asl (Cabak makao local variety, the widest stem diameter is at 50 meters asl (Cabak makao local variety) the longest leaf and branch is at 300 meters asl (Cabak makao local variety) and 1000 meters asl (Cabak makao local variety). There is no difference of anatomy in the root, stem, and leaf and no difference of protein band pattern either in Adira1 or Cabak makao local variety.
Activity of Zymomonas mobilis on ethanol products made of cashew nut apple (Anacardium occidentale) with different sources of nitrogen AKHMAD MUSTOFA; SURANTO SURANTO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010301

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Abstract. Mustofa A, Suranto. 2009. Activity of Zymomonas mobilis on ethanol production made of cashew nut apple (Anacardium occidentale) with different sources of nitrogen. Nusantara Bioscience 1: 105-109. This research is aimed at identifying Zymomonas mobilis in producing ethanol through batch fermentation process (in 24, 48 and 72 hours) using cashew nut apple extract (red, green and yellow variety) and urea, ammonium sulfate, extract of green peanut sprout and extract koro (Mucuna pruriens) as sources of nitrogen. The research showed that green cashew nut extract with ammonium sulfate in 24 hours of fermentation produced ethanol in optimum result. This treatment had pH of 5.87, 7.64 g/100 mL of sugar (with 48.44% of consumption), 8.0x107 amount of bacterium (µ = 0.154) and production of ethanol equal to 33.02 g/L (Ye = 90.19%).
Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties DWI HASTUTI; SURANTO SURANTO; PRABANG SETYONO
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010205

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Hastuti D, Suranto, Setyono P. 2009. Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties. Nusantara Bioscience 1: 78-83. The aim of this research to find out the Adenium obesum variation from six varieties, namely: obesum, cery, red lucas, red fanta , white bigben and harry potter based on morphology, karyotype, as well as protein banding pattern. The chromosome preparation was made using semi-permanent squash method from the tip of root plant; while protein banding pattern was made using SDS-PAGE method. Qualitative data included shape and color of the leave and flower described from each variety. Data were presented in morphometry and analyzed using ANOVA and then followed by DMRT with 5% of confidence levels, indicated significance difference. Protein banding pattern, the root, stem, leave and all organs were analyzed using Hierarchical Cluster Analysis method with Average Linkage (between Groups) using SPSS 10.0. The result of research shows that the six A. obesum varieties have morphological character with no variation of light green to dark green leave, not hairy, smooth leave bone, meanwhile for light red to dark red flower crown color although some of them are white and the same funnel color, yellow. All varieties of A. obesum have same number of chromosome, 2n = 22 and shows the difference ranging from 2.56 to 5.13 μm. In the banding pattern formed qualitatively, there is variation among the six varieties.
Co-Authors A.T. SYAHIDAH Achmad Achmad Adriana Grahani Firdaussy Agung Wahyudi Agus Cahyono Agus Riwanto AHMAD DWI SETYAWAN Ahmad Kailani, Ahmad AINUN NIKMATI LAILY AKHMAD MUSTOFA Alanindra Saputra Andina Elok Puri Maharani ANGELIA ARUM PRAMETA Anggara, Reksi Anggreyani, Reysa ANI SULISTYARSI Annisa Dewi Setyowati Anshori, Mahfud Ari Susilowati ARI SUSILOWATI Ari Pitoyo ARI PITOYO Ari Pitoyo ARI PITOYO ARI PITOYO ARI PITOYO ARI SUSILOWATI Aria Dirawan Arief A. Aryanto Budhy Sulihyantoro Aton Rustandi Mulyana BAMBANG SUDARMANTO Bambang Sunarto Baskoro Adi Prayitno Bengke, Melki Bertha Wikara Bintarto, Bambang Chatarina Heny Dwi Surwati Chusnu Syarifa Diah Kusuma Darmaka, Benediktus James Widya Deniawan Tommy Candra Dermawan, Dika Dewi Sekar Kencono Dhany Efita Sari Dwi Harsono DWI HASTUTI Dwi Tiyanto DWI WAHYUNI EDI PURWANTO EDWI MAHADJOENO EDWI MAHADJOENO Edwi Mahajoeno EDY PURWANTO Eka Nada Shofa Alkhajar Erham Budi Wiranto Erlina, Yenny Herawati Yohana Heylen EVI GRAVITIANI FARAH AULYA FARADILLA Farah Aulya Faradilla Farah Aulya Faradilla Fatmahwati, Seli Fauzi, Achmad Noor Firdastin Ruthnia Yudiningrum Fitriana Nur Astuti Gatot Wibowo Handayani, Arinda HAPSARI SETIA PUTRI Helmy, Mia Ferian Hendro Gunadi Henricus Hans Setyawan Prabowo Henricus Hans Setyawan Prabowo Hidayati, Muslihah Nur Husodo, Jadmiko Anom I. L. G. Nutjahjaningsih Ida Untari Idam Ragil Widianto Atmojo Ijah Mulyani Sihotang Imran, Muhammad Ali Ina Primasari Ina Primasari Isharyanto Isharyanto ISTIANA PRIHATINI Istiana Prihatini JOKO SUTRISNO Khotimah, Ainul Khotimah Lalu Usman Ali Lardianto Budhi Lesmana, Yeffry hendra MARSUSI MARSUSI Mauro Mocerino Meti Indrowati MOHAMMAD MASYKURI Mohammad Masykuri MUHAMMAD FURQAN MUHAMMAD FURQAN Muhammad Fahmi Johan Syah Muhammad Maga Sule Muhammad RIDWAN Murni Ramli Murni Ramli MUZZAZINAH MUZZAZINAH MUZZAZINAH MUZZAZINAH Muzzazinah Muzzazinah Nagoor Gafoordeen Nita Etikawati Novita Wulandari NUR RAHMAH HIDAYATI NUR RAHMAH HIDAYATI NURLIANI BERMAWIE NURLIANI BERMAWIE Oktaviariesta Habibatus Sholikhah PRABANG SETYONO PRABANG SETYONO Pramana, Edho Purin Candra Purnama PURNOMO PURNOMO PURNOMO PURNOMO Qinyu Yu Rafidiyah, Dina Rahmadhini, RR Rahajeng Suryo RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RESTYKANIA Retno Dwi Suyanti Ria Arista Asih Riezky Maya Probosari Rinawaty Rochman Hadi Mustofa Rustopo Rustopo Sabar Narimo Sajidan SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN Salma Azzah Fitran Saputri Sanastiti, Zahwa Sari Wijayanti Sarwanto Sarwanto SEPTIANA KURNIASARI Siregar, Yani Sukriah SITI KHOIRIYAH Siti Rodhiah Azhari SLAMET SANTOSA Slamet Santosa SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA Solichatun Solichatun Solichatun Solichatun SOLIKAH ANA ESTIKOMAH Sri Handayani Sri Handayani Sri Handayani Sri Herwindya Baskara Wijaya sri herwindya baskara wijaya SRI MULYANI Sri Wahyuni SUDARMONO SUDARMONO SUGIYARTO SUGIYARTO SUGIYARTO SUGIYARTO Sukodoyo Sukodoyo Sularso Sularso Sulasmi Sulasmi Sunarto Sunarto Sunny Ummul Firdaus SUNTORO SUNTORO SUPRIYADI SUPRIYADI Supyani Supyani Supyani Supyani suratman SURATMAN SURATMAN SURATMAN SURATMAN Suratman Suratman SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN Suratman, Suratman Sutarno Sutarno SUTARNO SUTARNO Sutarno Sutarno Tanjung Ardo TIAS HESTI KUSUMAWATI TJAHJADI PURWOKO TJAHJADI PURWOKO Tri Saputra Medhācitto TRIBADI TRIBADI TUNJUNG SULAKSONO Umi Fatmawati UMI FATMAWATI Umi Fatmawati Veronika, Nofita Wahyu Pangestuning Astuti Wahyuni Purnami Wicaksono, Dimas Suryo Widya Mudyantini Widya Mudyantini WIRYANTO WIRYANTO Zakarijah, Edi Zufahmi Zufahmi Zulkarnain Mistortoify