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Suranto
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Sebelas Maret University Surakarta
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Short Communication:Comparisons of isozyme diversity in local Java cardamom (Amomum compactum) and true cardamom (Elettaria cardamomum) AHMAD DWI SETYAWAN; WIRYANTO WIRYANTO; SURANTO SURANTO; NURLIANI BERMAWIE; SUDARMONO SUDARMONO
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060114

Abstract

Setyawan AD, Wiryanto,Suranto, Bermawie N, Sudarmono. 2014. Comparisons of isozyme diversity in local Java cardamom (Amomum compactum) and true cardamom (Elettaria cardamomum). Nusantara Bioscience 6: 94-101.Fruits of Java cardamoms (Amomum compactum) and true cardamoms (Elettaria cardamomum) had long been used as spices, flavoring agent, garnishing plants etc. This research was conducted to find out: (i) variation of isozymic bands in some population of Java cardamoms and true cardamoms; and (ii) phylogenetic relationship of these cardamoms based on variation of isozymic bands. Plant material (i.e. rhizome) of Java cardamoms was collected from Bogor Botanical Garden, and plant material of true cardamoms was gathered from Indonesian Medicinal and Aromatic Crops Research Institute, Bogor, Indonesia. Ten accessions were assayed in every population. The two isozymic systems was assayed, namely esterase (EST) and peroxidase (PER, PRX). Phylogenetic relationship was determined by UPGMA method. The results showed that esterase gave nine isozymic bands, i.e. Rf 0.15, 0.26, 0.29, 0.33, 0.38, 0.42, 0.45, 0.53, and Rf 0.58., while peroxidase gave 10 isozymic bands, i.e. Rf 0.06, 0.14, 0.18, 0.22, 0.26, 0.29, 0.32, 0.37, 0.41, and Rf 0.46. Relationship dendrogram indicated that the number of data will affect the grouping based on species similarity; and more data was increasingly apparent in groupings; within these groups there were variations among its members.
The total protein band profile of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus ANI SULISTYARSI; SURANTO SURANTO; SUPRIYADI SUPRIYADI
Nusantara Bioscience Vol. 4 No. 1 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040107

Abstract

Abstract. Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Nusantara Bioscience 4: 32-35. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer mercapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108 and 117 kDa. These proteins are presumably from the group of ?-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference in the protein expression in those vectors, but slightly thicker band were observed in the infected leaves.
Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern AINUN NIKMATI LAILY; SURANTO SURANTO; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 4 No. 1 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040104

Abstract

Abstract. Laily AN, Suranto, Sugiyarto. 2012. Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern. Nusantara Bioscience 4: 16-21. Carica pubescens Lenne & K. Koch is a species of fruit plant firstly cultivated in South America and has adapted to the highland environment, such as Dieng Plateau, Central Java (±2000 m asl). It has a narrow habitat range and limited or unknown intraspecies variation. Therefore, important information about the characters of the plants at various altitudes is needed, so that it is possible to extend its distribution through transplantation to other areas. Characterization can be performed based on morphological characters, chemical content, and protein banding patterns. This study aimed to describe the morphological characters, the chemical content (antioxidant capacity), and the pattern of protein bands by staining, using Coomassie brilliant blue on C. pubescens in the Dieng Plateau. The research was conducted in the villages of Kejajar (1400 m asl), Patak Banteng (1900 m asl.), and Sembungan (2400 m asl.). The observations of morphological characters were conducted in the field and continued in the laboratory. Morphological characters, the chemical content (antioxidant capacity), and the banding pattern of protein of C. pubescens were analyzed descriptively. The results showed that the morphological characters of C. pubescens in Dieng Plateau varied in stems, leaves, and fruits. The antioxidant capacity decreased with decreasing habitat altitude, 2400 m asl. altitude> 1900 m altitude> 1400 m asl.. The Protein banding patterns did not vary, but the pattern in C. Papaya was different. The uniformity of the pattern of protein bands showed that genetic stability in C. pubescens was not affected by environmental factors.
Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java TRIBADI TRIBADI; SURANTO SURANTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020103

Abstract

Abstract. Tribadi, Suranto, Sajidan. 2009. Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java. Nusantara Bioscience 2: 14-22. This research is intended to find out the morphological and anatomical variation as well as the protein band pattern of cassava (Manihot esculenta Crantz) widely spread in three different areas of height. The sample collecting is done using simple random sampling in the three different areas of height that is 50, 300, 1000 meters asl in Ngawi District, East Java while the analysis of protein band pattern is done using SDS-PAGE. The result of the research of morphology and anatomy is analyzed descriptively and presented in the form of tables, histograms, and figures. The analysis of protein band pattern is done using quantitative and qualitative analysis that is based on the appearance or not the gel band pattern by counting the molecular weights based on code marker S 8445 and qualitative method based on the quality of the band formed. The band pattern formed is istimated and presented in the form of zimogram. The result of the research shows that the height of the cultivating site very much influences toward variations of root, stem and leaf morphology. The longest root is at 50-meter heights asl (Cabak makao local variety, the widest stem diameter is at 50 meters asl (Cabak makao local variety) the longest leaf and branch is at 300 meters asl (Cabak makao local variety) and 1000 meters asl (Cabak makao local variety). There is no difference of anatomy in the root, stem, and leaf and no difference of protein band pattern either in Adira1 or Cabak makao local variety.
Activity of Zymomonas mobilis on ethanol products made of cashew nut apple (Anacardium occidentale) with different sources of nitrogen AKHMAD MUSTOFA; SURANTO SURANTO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010301

Abstract

Abstract. Mustofa A, Suranto. 2009. Activity of Zymomonas mobilis on ethanol production made of cashew nut apple (Anacardium occidentale) with different sources of nitrogen. Nusantara Bioscience 1: 105-109. This research is aimed at identifying Zymomonas mobilis in producing ethanol through batch fermentation process (in 24, 48 and 72 hours) using cashew nut apple extract (red, green and yellow variety) and urea, ammonium sulfate, extract of green peanut sprout and extract koro (Mucuna pruriens) as sources of nitrogen. The research showed that green cashew nut extract with ammonium sulfate in 24 hours of fermentation produced ethanol in optimum result. This treatment had pH of 5.87, 7.64 g/100 mL of sugar (with 48.44% of consumption), 8.0x107 amount of bacterium (µ = 0.154) and production of ethanol equal to 33.02 g/L (Ye = 90.19%).
Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties DWI HASTUTI; SURANTO SURANTO; PRABANG SETYONO
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010205

Abstract

Hastuti D, Suranto, Setyono P. 2009. Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties. Nusantara Bioscience 1: 78-83. The aim of this research to find out the Adenium obesum variation from six varieties, namely: obesum, cery, red lucas, red fanta , white bigben and harry potter based on morphology, karyotype, as well as protein banding pattern. The chromosome preparation was made using semi-permanent squash method from the tip of root plant; while protein banding pattern was made using SDS-PAGE method. Qualitative data included shape and color of the leave and flower described from each variety. Data were presented in morphometry and analyzed using ANOVA and then followed by DMRT with 5% of confidence levels, indicated significance difference. Protein banding pattern, the root, stem, leave and all organs were analyzed using Hierarchical Cluster Analysis method with Average Linkage (between Groups) using SPSS 10.0. The result of research shows that the six A. obesum varieties have morphological character with no variation of light green to dark green leave, not hairy, smooth leave bone, meanwhile for light red to dark red flower crown color although some of them are white and the same funnel color, yellow. All varieties of A. obesum have same number of chromosome, 2n = 22 and shows the difference ranging from 2.56 to 5.13 μm. In the banding pattern formed qualitatively, there is variation among the six varieties.
Protein expression on Cr resistant microorganism using electrophoresis method UMI FATMAWATI; SURANTO SURANTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010105

Abstract

Abstract. Fatmawati U, Suranto, Sajidan. 2009. Protein expression on Cr resistant microorganism using electrophoresis method. Nusantara Bioscience 1: 31-37. Hexavalent chromium (Cr(VI)) is known as toxic heavy metals, so the need is reduced to Cr(III) is much less toxicity. Pseudomonas aeruginosa, Pseudomonas putida, Klebsiella pneumoniae, Pantoea sp., and Saccharomyces cerevisiae are resistant Cr(VI) microorganisms and can reduce Cr(VI). The aim of this research is to know ability of the microorganism to reduce Cr(VI) and to know the protein band pattern between Cr(VI) resistant microorganisms and non-resistant microorganisms inoculated on LB broth. SDS-PAGE was used to identify protein expression. At the same time, Cr(VI) concentration was identified by the 1.5 diphenyl carbazide method. The quantitative data were analyzed by two factorial ANOVA that continued with DMRT at a 1% level test. The qualitative data, i.e., protein expression analyzed by relative mobility (Rf). The results showed that the ability of microorganisms to reduce Cr(VI) at an initial concentration of 0.5 ppm, one ppm, five ppm, and 10 ppm might vary; the average percentage of the ability of each microorganism in reducing Cr(VI) is P. putida (65%) > S. cerevisiae (64.45%) >. P. aeruginosa (60.73%) > Pantoea sp. (50.22%) > K. pneumoniae (47.82%) > without microorganisms (34.25%).ListenRead phonetically The adding microorganisms have significantly influenced toward reduction of Cr(VI). The SDS-PAGE shows that protein expression between resistant and not resistant microorganisms are no different, but resistant microorganisms have more protein (protein band is thicker).
Inclusive Economic Empowerment for Santika's Disabled Groups Based on Digital Storytelling for Local Product Branding Sulasmi, Sulasmi; Suranto; Kusuma, Chusnu Syarifa Diah
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/aqavty17

Abstract

This Community Service program aims to enhance the capacity of disability groups in Kulon Progo by providing productive skills training, strengthening self-confidence, and delivering inclusive entrepreneurial mentoring. The program responds to several key challenges faced by partners, including limited access to disability-friendly vocational training, low levels of entrepreneurial literacy, inadequate marketing skills, and the absence of sustained mentoring after initial training activities. To address these issues, the program adopts a participatory and inclusive approach that actively involves participants in every stage of the process. The implementation methods include structured training sessions, hands-on practice tailored to participants’ needs and abilities, continuous mentoring, and systematic evaluation of participant satisfaction. This approach ensures that learning is practical, relevant, and empowering for all participants. The evaluation process is conducted using four primary indicators: the relevance and quality of training materials, the competence of resource persons, the adequacy of facilities and infrastructure, and the effectiveness of program implementation and follow-up activities. The findings reveal that participant satisfaction falls within the “very good” category across all indicators. Overall, this program has demonstrated a positive impact in increasing participants’ work motivation, improving basic entrepreneurial skills, fostering independence, and enhancing their readiness to engage in sustainable and more focused productive economic activities in the future.
The Impact of the Global Food Crisis and Indonesia's Strategies For Achieving Food Security Muhammad RIDWAN; Suranto SURANTO
International Journal of Environmental, Sustainability and Social Science Vol. 6 No. 6 (2025): International Journal of Environmental, Sustainability, and Social Science (Nov
Publisher : PT Keberlanjutan Strategis Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38142/ijesss.v6i6.1695

Abstract

The world food crisis, triggered by various factors such as climate change, geopolitical conflicts, and a global pandemic, has had significant impacts on food security in various countries, including Indonesia. This article aims to identify and analyze sustainable strategies that can be implemented to address the impacts of the global food crisis on Indonesian food security and achieve food self-sufficiency. This research uses a qualitative descriptive method with a literature review approach using relevant sources. The research results indicate that sustainable strategy efforts to achieve national food security and food self-sufficiency include: (1) Increasing the productivity of agricultural food commodity, (2) Expansion of agricultural areas, (3) Diversification of local carbohydrate food sources, (4) Optimizing marine resource, (5) Community empowerment, and (6) Policy support and holistic cooperation. Overall, national food security can be achieved through a holistic socio-economic approach involving various aspects, from stakeholders, academia, and society.
Evaluation of Electrical Submersible Pump (ESP) Based on Energy Loss Analysis At South-Eeast Sumatra (SES) Block Andika Rizky Febrianto; Suranto; KRT Nur Suhascaryo; Muhammad Nauval Rizki; Riko Meidiya Putra; Slamet Kurniansyah
Journal of Petroleum and Geothermal Technology Vol. 7 No. 1 (2026): May
Publisher : Universitas Pembangunan Nasional "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/jpgt.v7i1.16402

Abstract

The production wells in the South-East Sumatra (SES) Block are characterized as a mature field with a high water cut, requiring significant energy to lift fluids to the surface. The main challenge faced is the high energy inefficiency of the Electrical Submersible Pump (ESP). Therefore, this study aims to evaluate ESP performance based on energy loss analysis, determine the best optimization scenario to reduce energy requirements, and analyze potential operational cost savings. The research method involves evaluating the distribution of energy losses in hydraulic components (friction, backpressure, and pumps) and electrical components (motor, cables, and surface equipment). Based on this evaluation, three optimization scenarios were conducted: backpressure loss, frictional loss, and pump loss. Furthermore, a cost savings analysis is carried out by comparing the costs before and after optimization. The results indicate that energy inefficiency in high flow rates wells is dominated by backpressure and friction, while in relatively small flow rates wells is caused by low pump efficiency and backpressure. The application of the best optimization scenario in the five wells can reduce the total energy requirement from 768.83 kW to 568.21 kW. This resulted in a cost reduction from US$512,641 to US$378,869.
Co-Authors A.T. SYAHIDAH Achmad Achmad Adimas Maditra Permana Adriana Grahani Firdaussy Agung Wahyudi Agus Riwanto Ahimsa Adi Wibowo AHMAD DWI SETYAWAN Ahmad Kailani, Ahmad AINUN NIKMATI LAILY Akhmad Mustofa Alanindra Saputra Andika Rizky Febrianto Andina Elok Puri Maharani ANGELIA ARUM PRAMETA Anggara, Reksi Anggreyani, Reysa ANI SULISTYARSI Annisa Dewi Setyowati ARI SUSILOWATI Ari Susilowati ARI PITOYO Ari Pitoyo ARI PITOYO ARI PITOYO ARI PITOYO Ari Pitoyo Ari Pitoyo ARI SUSILOWATI Aria Dirawan Arief A. Aryanto Budhy Sulihyantoro BAMBANG SUDARMANTO Bambang Sunarto Baskoro Adi Prayitno Bengke, Melki Bertha Wikara Bintarto, Bambang Chusnu Syarifa Diah Kusuma Darmaka, Benediktus James Widya Deby Fitriana Deniawan Tommy Candra Deniawan Tommy Chandra Wijaya Dermawan, Dika Dewi Sekar Kencono Dhany Efita Sari Dina Selia Wardani Dwi Harsono DWI HASTUTI Dwi Tiyanto DWI WAHYUNI EDI PURWANTO EDWI MAHADJOENO EDWI MAHADJOENO Edwi Mahajoeno EDY PURWANTO Eka Nada Shofa Alkhajar Erham Budi Wiranto Erlina, Yenny Herawati Yohana Heylen Evi Gravitiani FARAH AULYA FARADILLA Farah Aulya Faradilla Farah Aulya Faradilla Fatmahwati, Seli Fauzi, Achmad Noor Fitriana Nur Astuti Gatot Wibowo Handayani, Arinda Hanifah Islamiyah HAPSARI SETIA PUTRI Helmy, Mia Ferian Hendro Gunadi Henricus Hans Setyawan Prabowo Henricus Hans Setyawan Wibowo Hidayati, Muslihah Nur Husna nur Amalina Husodo, Jadmiko Anom I. L. G. Nutjahjaningsih Ida Untari Idam Ragil Widianto Atmojo Iim Fathimah Ijah Mulyani Sihotang Imran, Muhammad Ali Ina Primasari Ina Primasari Irianti, Indah Susi Isharyanto Isharyanto ISTIANA PRIHATINI Istiana Prihatini Joko Sutrisno Khotimah, Ainul Khotimah KRT Nur Suhascaryo Lalu Usman Ali Lesmana, Yeffry hendra MARSUSI MARSUSI Mauro Mocerino Meti Indrowati MOHAMMAD MASYKURI Mohammad Masykuri MUHAMMAD FURQAN MUHAMMAD FURQAN Muhammad Fahmi Johan Syah Muhammad Maga Sule Muhammad Nauval Rizki Muhammad RIDWAN Murni Ramli Murni Ramli MUZZAZINAH MUZZAZINAH MUZZAZINAH MUZZAZINAH Muzzazinah Muzzazinah Nagoor Gafoordeen Nita Etikawati Novita Wulandari NUR RAHMAH HIDAYATI NUR RAHMAH HIDAYATI NURLIANI BERMAWIE NURLIANI BERMAWIE Oktaviariesta Habibatus Sholikhah PRABANG SETYONO PRABANG SETYONO Pramana, Edho Purin Candra Purnama Purin Candra Purnama PURNOMO PURNOMO PURNOMO PURNOMO Rafidiyah, Dina Rahmadhini, RR Rahajeng Suryo RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RESTYKANIA Retno Dwi Suyanti Ria Arista Asih Riezky Maya Probosari Riko Meidiya Putra Rinawaty Rochman Hadi Mustofa Rustopo Rustopo Sabar Narimo Sajidan SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN Salma Azzah Fitran Saputri Sanastiti, Zahwa Sari Wijayanti Sarwanto Sarwanto SEPTIANA KURNIASARI Siregar, Yani Sukriah SITI KHOIRIYAH Siti Rodhiah Azhari Slamet Kurniansyah Slamet Santosa SLAMET SANTOSA SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA Solichatun Solichatun Solichatun Solichatun Solichatun Solichatun SOLIKAH ANA ESTIKOMAH Sri Handayani Sri Handayani Sri Handayani Sri Herwindya Baskara Wijaya sri herwindya baskara wijaya SRI MULYANI Sri Wahyuni SUDARMONO SUDARMONO SUGIYARTO SUGIYARTO SUGIYARTO SUGIYARTO Sukodoyo Sukodoyo Sulasmi Sulasmi Sumardi Sunarto Sunarto Sunny Ummul Firdaus SUNTORO SUNTORO SUPRIYADI SUPRIYADI Supyani Supyani Supyani Supyani suratman SURATMAN SURATMAN Suratman Suratman SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN Suratman, Suratman Sutarno Sutarno Sutarno Sutarno SUTARNO SUTARNO Tanjung Ardo TIAS HESTI KUSUMAWATI TJAHJADI PURWOKO TJAHJADI PURWOKO TJAHJADI PURWOKO Tri Saputra Medhācitto TRIBADI TRIBADI TUNJUNG SULAKSONO UMI FATMAWATI Umi Fatmawati Umi Fatmawati Veronika, Nofita Vionita Dyah Pramesthi Wahyu Haspri Nur Taryanti Wahyu Pangestuning Astuti Wahyuni Purnami Wicaksono, Dimas Suryo Widya Mudyantini Widya Mudyantini Widya Mudyantini WIRYANTO WIRYANTO Zakarijah, Edi Zufahmi Zufahmi