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Protein expression on Cr resistant microorganism using electrophoresis method UMI FATMAWATI; SURANTO SURANTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010105

Abstract

Abstract. Fatmawati U, Suranto, Sajidan. 2009. Protein expression on Cr resistant microorganism using electrophoresis method. Nusantara Bioscience 1: 31-37. Hexavalent chromium (Cr(VI)) is known as toxic heavy metals, so the need is reduced to Cr(III) is much less toxicity. Pseudomonas aeruginosa, Pseudomonas putida, Klebsiella pneumoniae, Pantoea sp., and Saccharomyces cerevisiae are resistant Cr(VI) microorganisms and can reduce Cr(VI). The aim of this research is to know ability of the microorganism to reduce Cr(VI) and to know the protein band pattern between Cr(VI) resistant microorganisms and non-resistant microorganisms inoculated on LB broth. SDS-PAGE was used to identify protein expression. At the same time, Cr(VI) concentration was identified by the 1.5 diphenyl carbazide method. The quantitative data were analyzed by two factorial ANOVA that continued with DMRT at a 1% level test. The qualitative data, i.e., protein expression analyzed by relative mobility (Rf). The results showed that the ability of microorganisms to reduce Cr(VI) at an initial concentration of 0.5 ppm, one ppm, five ppm, and 10 ppm might vary; the average percentage of the ability of each microorganism in reducing Cr(VI) is P. putida (65%) > S. cerevisiae (64.45%) >. P. aeruginosa (60.73%) > Pantoea sp. (50.22%) > K. pneumoniae (47.82%) > without microorganisms (34.25%).ListenRead phonetically The adding microorganisms have significantly influenced toward reduction of Cr(VI). The SDS-PAGE shows that protein expression between resistant and not resistant microorganisms are no different, but resistant microorganisms have more protein (protein band is thicker).
Inclusive Economic Empowerment for Santika's Disabled Groups Based on Digital Storytelling for Local Product Branding Sulasmi, Sulasmi; Suranto; Kusuma, Chusnu Syarifa Diah
Jurnal Ragam Pengabdian Vol. 3 No. 2 (2026): Mei-Agustus, Sustainable Development Goals (SDGs): Multidisciplinary Perspectiv
Publisher : Lembaga Teewan Journal Solutions

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62710/aqavty17

Abstract

This Community Service program aims to enhance the capacity of disability groups in Kulon Progo by providing productive skills training, strengthening self-confidence, and delivering inclusive entrepreneurial mentoring. The program responds to several key challenges faced by partners, including limited access to disability-friendly vocational training, low levels of entrepreneurial literacy, inadequate marketing skills, and the absence of sustained mentoring after initial training activities. To address these issues, the program adopts a participatory and inclusive approach that actively involves participants in every stage of the process. The implementation methods include structured training sessions, hands-on practice tailored to participants’ needs and abilities, continuous mentoring, and systematic evaluation of participant satisfaction. This approach ensures that learning is practical, relevant, and empowering for all participants. The evaluation process is conducted using four primary indicators: the relevance and quality of training materials, the competence of resource persons, the adequacy of facilities and infrastructure, and the effectiveness of program implementation and follow-up activities. The findings reveal that participant satisfaction falls within the “very good” category across all indicators. Overall, this program has demonstrated a positive impact in increasing participants’ work motivation, improving basic entrepreneurial skills, fostering independence, and enhancing their readiness to engage in sustainable and more focused productive economic activities in the future.
Co-Authors A.T. SYAHIDAH Achmad Achmad Adriana Grahani Firdaussy Agung Wahyudi Agus Cahyono Agus Riwanto AHMAD DWI SETYAWAN Ahmad Kailani, Ahmad AINUN NIKMATI LAILY AKHMAD MUSTOFA Alanindra Saputra Andina Elok Puri Maharani ANGELIA ARUM PRAMETA Anggara, Reksi Anggreyani, Reysa ANI SULISTYARSI Annisa Dewi Setyowati Anshori, Mahfud Ari Susilowati ARI SUSILOWATI Ari Pitoyo ARI PITOYO Ari Pitoyo ARI PITOYO ARI PITOYO ARI PITOYO ARI SUSILOWATI Aria Dirawan Arief A. Aryanto Budhy Sulihyantoro Aton Rustandi Mulyana BAMBANG SUDARMANTO Bambang Sunarto Baskoro Adi Prayitno Bengke, Melki Bertha Wikara Bintarto, Bambang Chatarina Heny Dwi Surwati Chusnu Syarifa Diah Kusuma Darmaka, Benediktus James Widya Deniawan Tommy Candra Dermawan, Dika Dewi Sekar Kencono Dhany Efita Sari Dwi Harsono DWI HASTUTI Dwi Tiyanto DWI WAHYUNI EDI PURWANTO EDWI MAHADJOENO EDWI MAHADJOENO Edwi Mahajoeno EDY PURWANTO Eka Nada Shofa Alkhajar Erham Budi Wiranto Erlina, Yenny Herawati Yohana Heylen EVI GRAVITIANI FARAH AULYA FARADILLA Farah Aulya Faradilla Farah Aulya Faradilla Fatmahwati, Seli Fauzi, Achmad Noor Firdastin Ruthnia Yudiningrum Fitriana Nur Astuti Gatot Wibowo Handayani, Arinda HAPSARI SETIA PUTRI Helmy, Mia Ferian Hendro Gunadi Henricus Hans Setyawan Prabowo Henricus Hans Setyawan Prabowo Hidayati, Muslihah Nur Husodo, Jadmiko Anom I. L. G. Nutjahjaningsih Ida Untari Idam Ragil Widianto Atmojo Ijah Mulyani Sihotang Imran, Muhammad Ali Ina Primasari Ina Primasari Isharyanto Isharyanto ISTIANA PRIHATINI Istiana Prihatini JOKO SUTRISNO Khotimah, Ainul Khotimah Lalu Usman Ali Lardianto Budhi Lesmana, Yeffry hendra MARSUSI MARSUSI Mauro Mocerino Meti Indrowati MOHAMMAD MASYKURI Mohammad Masykuri MUHAMMAD FURQAN MUHAMMAD FURQAN Muhammad Fahmi Johan Syah Muhammad Maga Sule Muhammad RIDWAN Murni Ramli Murni Ramli MUZZAZINAH MUZZAZINAH MUZZAZINAH MUZZAZINAH Muzzazinah Muzzazinah Nagoor Gafoordeen Nita Etikawati Novita Wulandari NUR RAHMAH HIDAYATI NUR RAHMAH HIDAYATI NURLIANI BERMAWIE NURLIANI BERMAWIE Oktaviariesta Habibatus Sholikhah PRABANG SETYONO PRABANG SETYONO Pramana, Edho Purin Candra Purnama PURNOMO PURNOMO PURNOMO PURNOMO Qinyu Yu Rafidiyah, Dina Rahmadhini, RR Rahajeng Suryo RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RATNA SETYANINGSIH RESTYKANIA Retno Dwi Suyanti Ria Arista Asih Riezky Maya Probosari Rinawaty Rochman Hadi Mustofa Rustopo Rustopo Sabar Narimo Sajidan SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN SAJIDAN Salma Azzah Fitran Saputri Sanastiti, Zahwa Sari Wijayanti Sarwanto Sarwanto SEPTIANA KURNIASARI Siregar, Yani Sukriah SITI KHOIRIYAH Siti Rodhiah Azhari SLAMET SANTOSA Slamet Santosa SOERYA DEWI MARLIYANA SOERYA DEWI MARLIYANA Solichatun Solichatun Solichatun Solichatun SOLIKAH ANA ESTIKOMAH Sri Handayani Sri Handayani Sri Handayani Sri Herwindya Baskara Wijaya sri herwindya baskara wijaya SRI MULYANI Sri Wahyuni SUDARMONO SUDARMONO SUGIYARTO SUGIYARTO SUGIYARTO SUGIYARTO Sukodoyo Sukodoyo Sularso Sularso Sulasmi Sulasmi Sunarto Sunarto Sunny Ummul Firdaus SUNTORO SUNTORO SUPRIYADI SUPRIYADI Supyani Supyani Supyani Supyani suratman SURATMAN SURATMAN SURATMAN SURATMAN Suratman Suratman SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN SURATMAN Suratman, Suratman Sutarno Sutarno SUTARNO SUTARNO Sutarno Sutarno Tanjung Ardo TIAS HESTI KUSUMAWATI TJAHJADI PURWOKO TJAHJADI PURWOKO Tri Saputra Medhācitto TRIBADI TRIBADI TUNJUNG SULAKSONO Umi Fatmawati UMI FATMAWATI Umi Fatmawati Veronika, Nofita Wahyu Pangestuning Astuti Wahyuni Purnami Wicaksono, Dimas Suryo Widya Mudyantini Widya Mudyantini WIRYANTO WIRYANTO Zakarijah, Edi Zufahmi Zufahmi Zulkarnain Mistortoify