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HEMATOLOGICAL PROFILES OF AFRICAN CATFISH (Clarias gariepinus) THAT ARE MAINTAINED BY BIOFLOC SYSTEMS Kurniawan, Ahmad Juli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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                                              ABSTRACTThe researcher conducted this research from November to December 2018 in two places: Ambon Farm Pekanbaru and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine Sciences, Riau University. This research aims to obtain information about hematological profiles of African catfish that are maintained by biofloc systems. The research method used in this study is the survey method. From the sampling location, ware collected as much as 30 African catfish (15 male and 15 female), with a length of 20-25 cm.Sampling was done three times with a span of 14 days. Every sampling ware collected as much as 10 African catfish (5 male and 5 female). Blood sampling is done directly at location and hematological parameter measured at Laboratory. Total erythrocyte and leukocyte measured were Klonz (1994), hemoglobin levels (Wademeyer and Yasutake, 1977), haematocrit and leucocrit levels (Anderson and Siwicki, 1995). The results shows that the hematological value of male African catfishs, total erythrocytes 197.7 x104 cells/mm3, hematocrit 24.4%, hemoglobin 7.3 g/dL, total leukocyte 236.6 x103 cells/mm3, leukocrit 2.86%, while the total erythrocyte of female African catfish is 195.6 x104 cells/mm3, hematocrit 23.7%, hemoglobin 6.7 g/dL, leukocyte total 222.2 x103 cells/mm3, leukocrit 2.26%. Water quality parameters of bioflok ponds during as research were in the ranges, temperature 25-260C, pH 6.7-71, dissolved oxygen 2.8-3.1 mg/L, dan ammonia 0.958-1.235 mg/L.Key Words : Hematological, Clarias gariepinus, Biofloc                                           
ERYTROCYTE PFOFAILE AND SURVIVAL OF Pangasius hypophthalmusFED WITHRhizophora apiculata EXTRACT ENRICHED PELLETS Anderson.s, Roi; Syawal, Henni; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This study aims to look at the description of erythrocyte and tehe ability of feed containing Rhizophora apiculata leaf extract to improve the survival of Siam jambal fish (Pangasius hypophthalmus).The method used is the experimental method with Completely Randomized Design (CRD), one factor with 5 levels of treatment and three replications. Kn: Negative control (feeding without being given R. apiculata leaf extract and without being challenged with A. hydrophila bacteria), Kp: Positive control (feeding without being given R. apiculata leaf extract and tested challenged with A. hydrophila bacteria), P1: Feed containing R. apiculata leaf extract at a dose of 1.5 mg/kg of feed, P2: Dose of 1.6 mg/kg of feed, P3: Dose of 1.7 mg/kg of feed. Siam Jambal fish (P. hypophthalmus) size 8-10 cm is maintained in an aquarium measuring 40×30×30cm with a stocking densities of 1 tail / 3L of water,feeding  is done three times a day as much as 10 % of fish body weight.Taking the blood of the tested fishes was done 3 times, namely at the beginning of maintenance, after 30 days of maintenance and 14 days after the test challenged with A. hydrophila. The results of research show that P3 (1.7 mg/kg addition of R. apiculata leaf extract) was the best dose, to improve fish health seen from the final value of total erythrocytes 2.78×106 cells/mm3,hematocrit value 37.00%, and hemoglobin levels from 12.00 g/dL after the challenge test and produce an 80% survival rate.Keywords:Erythrocyte,Hematocrit,Hemoglobin, Pangasius hypophthalmus, Rhizophora apiculata, Aeromonas hydrophila.
PREVENTION MotilAeromonas Septicemia (MAS) of Pangasius hypophthalmus by SOLUTION Citrus hystrix Santa, Santa; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTCitrus hystrix is natural ingredient that was potentially being antimicrobial, because having many flavonoid, tannin and alkaloid compounds. The aim of this study was to get the best doses of solution Citrus hystrixto prevent MAS seen by leukocyte changes of Pangasiushypopthalmusso that it can be applied to fish affected by MAS disease. The method used of this study was Complete Random Match (CRD) with 1 factor, 5 treatments and 3 replications. NamelyKn negative control (without soaking solution Citrus hystrixand not challengedbyAeromonas hydrophila), Kp positive control (soaking solution Citrus hystrixand challengedbyAeromonas hydrophila), P1 soaking solution Citrus hystrixby 1200 ppm and challengedbyAeromonas hydrophila, P2 soaking solution Citrus hystrixby 1500 ppm and challengedbyAeromonas hydrophila,P3soaking solution Citrus hystrixby1800  ppm and challengedbyAeromonas hydrophila. Prevention by soaking for 2 minutes and done 4 times with interval of 7 days.Pangasiushypopthalmusused with 6-12 cm of size and 150 fishes. The study showed that soaking solution Citrus hystrixwith 1500 ppm of dose increased Pangasiushypopthalmushealth, seen by 10,26x104cells/mm3of leukocyte averages, leukocyte differentiations (85,67 ofLymphocytes, 5,67% ofneutrophils, 5,67% ofmonocytes,37,00%of phagocyte activities, 100%of survival rate dan 11,33 g of absolute weight growth. Keyword : Leukocyte, Pangasius hypophthalmus, Citrus hystrix,Aeromonas  hydrophila, Soaking
SENSITIVITY SOLUTION OF KERSEN LEAVES (Muntingia calabura L.) AGAINST BACTERIAAeromonas Wijaya, Rahmat Santosa; Lukistyowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Disease that often develops in aquaculture is one of them is red spot disease.This disease is often called Motile Aeromonas Septicemia (MAS) caused byAeromonas hydrophila.Natural ingredients that can be used as prevention and treatment of fish diseases are kersen leaf plant (Muntingia calabura L) which is antibacterial and antimicrobial.This research was conducted in December 2018-February 2019 in the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. The purpose of study was to determine the sensitivity of solution of kersen leaves to inhibit the growth of bacteria Aeromonas hydrophila and to know LD50solution of kersen leaves to jambal siam (Pangasius hypophthalmus) by immersion. Research method used is the experimental method, for sensitivity testusing the method of Kirby Bauerdisc with dosage used were 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 9%, 8%, 7%, 6%, 5% , 4% and controls (Oxytetracyclin). The results showed that the solution of kersen leaves can inhibit the growth of bacteriaA. hydrophila starting from a dose of 100% -4% with an average diameter of inhibition zones ranging from 13.55 - 6.25 mm. LD50 test of the solution of kersen leaf (M. calabura L.) againstjambal siam (P. hypophthalmus) by immersion for 24hoursis at a dose of 3.6%. Keywords: M. calabura L., Motil Aeromonas Septicemia, Kirby Bauer discs, LD50
THE CURATIVE METHODS OF MAS (Motile Aeromonas Septicaemia) USE LEAF MANGROVE EXTRACT (Rhizophora sp) ON JAMBAL SIAM FISH (Pangasius hypophthalmus) Mastuti, Rini; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThe research was conducted in May until August 2017 in Parasites and Fish Disease Laboratory of Marine and Fisheries Faculty University of Riau,and also in the Integrated Chemical Laboratory of Mathematics and Natural Science of Health Faculty Riau Muhammadiyah University. The purpose of the study was todetermine the ability of Rhizophoraspto cure the disease which is caused by MAS (Motile Aeromonas Septicaemia) and showed from the profile of Siamese Shark (Pangasius hypophthalmus)erythrocyte. The method was an experimental method with a Completely Randomized Design (CRD), one factor with four treatments, there were : control without curing, 1300 ppm, 1600 ppm, and 1900 ppmrespectively. The immersions was applied twice in two days by leaf mangrove extract (Rhizophora s)p for about 5 minutes. The result showed that leaf  mangrove extract (Rhizophora sp) able to cure Siamese Shark from MAS (Motile Aeromonas Septicaemia) by the immersions method. The best dose was at 1600 ppm wich was shown by the total erythrocyte was 290 x 104 cell/mm3, haemoglobin level was 9,30 g/dL, haematocrit value was 29,66 %, and the survival rate was 84,61%.Keywords: Rhizophora sp, Motile Aeromonas Septicaemia, Haematology
Gambaran Darah Ikan Nila (Oreochromis niloticus) di Kolam Budidaya di Kecamatan Marpoyan Damai Kota Pekanbaru Riauwaty, Morina; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 21, No 1 (2016)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.21.1.1-6

Abstract

The aim of the research is to describe the blood of Tilapia that had been cultured in a fishpond since June to December 2015. The ponds were located in Marpoyan Subdistrict, Pekanbaru city. Twenty samples were collected as much as 20 fish that consisted of 10 male and 10 female with the size ranging from 9.5 – 18.2 cm. The blood of the fish was taken with syringe via caudal vena as much of 1 ml. The blood parameters then were calculated in term of the number of hematocrit, leucocyte, erythrocyte, and leukocyte. The results showed that the amount of hematocrit and leukokrit were ranged between 10 – 31 % and 1.78 – 3.10 % consecutively. The total cell of erythrocyte ranged between 1,170,000 – 2,190,000 cell/mm3, and the total of leukosit were about 81,000 – 105,000 cell/mm3. In addition, the types of leukocyte found at this study were hepatocyte (53- 86%), thrombocyte (25 – 39%), monocyte and neutrophil between 1 – 3 %.
Description of Erythrocytes of Striped Catfish (Pangasionodon hypophthalmus) Infected by Edwardsiella tarda Treated with Mango Leaf (Mangifera indica) Tungkup, Mariana Lumban; Syawal, Henni; Riauwaty, Morina
Jurnal Perikanan dan Kelautan Vol 26, No 1 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.62-69

Abstract

This research has been carried out from November to December 2019 at the Laboratory of Parasites and Fish Diseases at the Fisheries and Marine Faculty of Riau University. The aim of the study was to obtain the best dose of mango leaf solution for the treatment of striped catfish (P. hypophthalmus) infected with E. tarda, seen from the erythrocyte picture. The method used was an experiment with a Completely Randomized Design (CRD) of a factor of five treatment levels and replications three times. Test fish used were striped catfish measuring 8-12 cm, with a total of 150 fish. The treatments used for treatment were 1000 ppm, 1200 ppm, 1400 ppm, negative controls (not infected and not treated), and positive controls (infected and not treated). Parameters measured and observed among others; total erythrocytes, hemoglobin levels, hematocrit values, erythrocyte cell morphology and survival rate. The results showed that apple mango leaf solution (M. indica) was able to treat conjoined striped catfish infected with E. tarda by Emmersion method. The best treatment of 1200 ppm is characterized by an average total erythrocyte, mean total erythrocytes 149.33 x 104 cells / mm3, hemoglobin level 9.03 g / dL, hematocrit value 35.67% and the survival of the test fish after treatment is 86.67%. The results of water quality measurements during the study, namely temperature 27.00-27.46 0C, pH 6.1-6.77, dissolved oxygen 3.53-3.87 mg/L, and ammonia 0.02 mg/L.
EFFECT OF CURCUMIN FEEDING IN FEED FOR TREATING JAMBAL SIAM FISH (Pangasius hypopthalmus) INFECTED BACTERIA Aeromonas hydrophila Pakpahan, Poltak; Syawal, Henni; Riauwaty, Morina
Jurnal Perikanan dan Kelautan Vol 25, No 3 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.3.224-231

Abstract

Curcumin is one of the secondary metabolite compounds products that can increase the immune system of fish against bacterial attack. The purpose of this research was to find out the changes in the erythrocytes of a striped fish that were infectious bacteria A. hydrophila and treated with feeding containing curcumin and know the best dose of curcumin extract in the feed. This research was conducted in September to November 2019. The test fish used was the seed of a striped fish that measures 8-10 cm by 150 tails. Method used was the method of experimental and complete random design a factor of 5 levels of treatment and 3 times repeated, the treatment was Kn (feeding without the extract of curcumin and not infected with bacteria A. hydrophila), Kp (feeding without being given curcumin extract and infectious bacteria (A. hydrophila), P1 (addition of curcumin in feed at a dose of 0.7 g/kg), P2 (0.9 g/kg), P3 (of 1.1g/kg). The results shown that P3 treatment was the best treatment with a < 0.05 P where in a total of erythrocytes was 2.46 x 106 cells/mm3. Hemoglobin 9.4 g/dl, hematocrit value of 32.3%, and survival rate was 90%. Curcumin was able to treat fish from an A. hydrophila infection.
Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Tamba, Jaya Maruli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol 26, No 1 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.40-46

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Sensitivity of Harumanis Mango Seed (Mangifera indica L) Solution toward Aeromonas hydrophila Telaumbanua, Siti; Lukistyowati, Iesje; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 24, No 1 (2019)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.24.1.24-31

Abstract

Research was conducted from October to December 2017, at the labory Examination of Fish Disease, Fisheries and Marine Faculty of Universitas Riau. The Purpose of this study was to determine the sensitivity of harumanis mango seed (Mangifera indica L) as antimicrobial to A. hydrophila, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) harumanis manggo seed (M. indica L) solution against to catfish (Pangasius pangasius) (7-9 cm) by immersion. The research method used doses; D1: 100%; D2: 90%; D3: 80%; D4: 70%; D5: 60%; D6: 50%; D7: 40%; D8: 30%; D9: 20% D10: 10%, and K : Control (Oxytetracyclin).The results showed that mango seed solution was sensitive to A. hydrophila bacteri and was able to inhibit A. hydrophila up to 0.09% dose with inhibit zone of 7.31 mm. Minimum Inhibitory Concetrantion (MIC) is 0.1% (1000 ppm) number of colonies 270.66 CFU/ mL.The dose of LD50 is 2115,85 ppm
Co-Authors Abdul Alif Abdul Alif Addilah, M. Fikri Adelina &#039; Afra Azizah Afrina Putri Afrizal Tanjung Alfian &#039; Alfinda, Rudi Alif, Abdul Amraini Fitri Anang Widarsa Andarini Diharmi Anderson.s, Roi Andesra, Andesra Andi Almer Farrand Noor Andre A Saragih, Andre A Andre Manusun Purba Anggriani, Rara Dewi Angraika Dirta Annisa Dwi Putri Anugrah, Rio Persal Anzila Rizki W Muharram Asmika Harnalin Simarmata Athif Amirah Elza Atika Talitha Erman Atria Martina Azizah, Afra Cerlina, Maya Chandra Chandra Chania Arnata Christin Natalia Cici Adliana Darfia, Novreta Dea Switzelin H Destriman Laoli Devy P Lumban Gaol Dian Fitria M Dian Iriani Dian Puspitowati Dicky Azwar Lubis Dinar Mey Jasika H Dwi Indah Fransiska Elfira Stefani Priska Erikson Sahala Pardamean Etri Nopilita Faira Yovanie Fandi Rahmat Farkhan, Mochammad Farrand Noor, Andi Almer Fatihaturrahma, Annisya Fauzi Ahmad Lubis Faza, Farzana Fakhira Fazira, Meisya Amanda Febi Nabila Feri Julianto Fitria Sufriyani Fransiska, Dwi Indah Fricilia Zissalwa Gaol, Devy P Lumban Ginting, Kiki Dirgantara Gunawan B S Gusriansyah, Dimas H Herdini Hafil Kurnia Tama Hasbullah Zakky Hasugian, Sampang Hotramadi Herianti Rambe Heriyanti Heriyanti, Heriyanti Herparinda Novita Husna, Hidayatul Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukstyowati Iesje Lukystiowati Ilham Fajri Pohan Iman, Khasan Nur Ipa, Adha Irma Yunita Irsya, Habib Irwan Effendi Irwan Irwan Iskandar Putra Isma Mulyani Isna Sudirman Isna Sudirman Isnaini Lidya Rizki Jampil, Tirta Herlis Jan Stepfely Simanjuntak Jaya Maruli Tamba Juniarty Manurung Juniarty Manurung Juwita Lestari Karnila, Rahman Karnila Khairia, Khairia Kiky Dirgantara Ginting Kurniawan, Ahmad Juli Kusai, Kusai Latifah Hannum Lase Lestari, Rona Luksityowati, Iesje Luskityowati, Iesje M Riswan M Zaky Ditiya Putra M. Riswan M. Yanda, M. Yanda Manurung, Juniarty Mariana Lumban Tungkup Mariska, Refi Marwati &#039; Maulina Sawitri Maya Cerlina Maytra Wirdona Meisya Amanda Fazira Melin Siburian Memi Martin Ilmayati Mery Sukmiwati Mesi Susanti Mochammad Farkhan Morina Riauwaty Muhamad Latiful Khobir Muhammad Akram Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Azaldin Muhammad Muhajir Muhammad Muhajir, Muhammad Mutawakkil, M. Mufid Al Mutiara Mutiara Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nasril Ismail NASTITI KUSUMORINI Nelli Karolina Sitorus Novike Zullanda Selvi, Novike Zullanda Novita, Herparinda Novreta Ersyi Darfia Nur Addini Nur Aini Nur Asiah Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nurfitri, Maya Nurhasan Nurhasan Nurhasan Nurhasan, Nurhasan Oktavianti, Mila Pakpahan, Poltak Pardamean, Erikson Sahala Pertiwi, Zuwini Poltak Pakpahan Poltak Pakpahan Purba, Andre Manusun Purba, Yoshua Dewaman Mangapul Putra, Mutlas Ade Putra, Sanjaya Mandala Putri Noviayu Salsabila Hendri Putri, Annisa Dwi Putri, Atika Putri, Mega Novia Putri, Rianti RA, Sayyidina Abdul Qabidhi Rabil Yusuf Rabil Yusuf Rahmadona, Zikra Rahmat Iman Mainil Ramadhanita, Ramadhanita Rebeka Valensia Rhino Pamungkas Riauwaty , Morina Ridho Aldiokta RIDWAN AFFANDI Rika Sinaga Rinaldi Rinaldi Rini Mastuti Rio Persal Anugrah Risman Ferdiansyah Riswan, M Riva Mutiara Rizka Hasanah Rizki Putra Rizvi, Fharisa Nabila Rona Lestari Ronal Kurniawan Roslina &#039; Rudi Alfinda Saberina Hasibuan Sagitha Aulya Fanny Tanjung Salsabila Hendri, Putri Noviayu Santa, Santa Santikawati, Susi Sarah Pabiola Saragih Siallagan, Romensius Anggi AW Siburian, Melin Simanjuntak, Jan Stepfely Sinaga, Tomuso Angdi Suryani Siregar, Cici YP Siregar, Desty Sahroni Siregar, Morina Riauwaty Sri Wahyuni Suci Wulandari Sudirman, Isna Syafriadiman, Syafriadiman Syafruddin Nasution Syahputra, Mohamad Ardani Syahrul Hidayat Syaieba, Mutiara Tamba, Jaya Maruli Tanjung, Yuliza Virliansyah Telaumbanua, Siti Tia Suherianti Tjipto Leksono Tjipto Leksono Tomuso Angdi Suryani Sinaga Trisla Warningsih Tungkup, Mariana Lumban Ulandari, Ulfa Unggul Fitrah Heriadi Usman M Tang Usman M Tang Usman Muhammad Tang Vekri Ramadhi Wasmen Manalu Widarsa, Anang Wijaya, Rahmat Santosa Windarti Windarti Yetti Elfina Yetti Elfina S Yoshua Demawan Mangapul Purba Yosri Yosri Yovanie, Faira Yuharmen - Yuliati Yuliati Yulyawati Yulyawati Yunita Paramitha Hasibuan Yusni Ikhwan Yusni Ikhwan Siregar Yusuf, Rabil Zebua, Ratna Dewi Zikra Rahmadona Zissalwa, Fricilia Zulaiha &#039; Zulkarnain Zulkarnain Zulkarnain Zulkarnain