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Lead Levels in Hair and Blood of Container Truck Drivers at PT. Trans Indo Sakti Surabaya Juliana Christyaningsih; Amiliya Antika Dewi; Ayu Puspitasari
International Journal of Advanced Health Science and Technology Vol. 3 No. 4 (2023): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i4.285

Abstract

Lead is one of the heavy metals used as an additive in gasoline to enhance lubrication and combustion efficiency in motor vehicles, such as container truck drivers, and is commonly found on highways. Truck drivers have a higher tendency to inhale air containing lead while on the road, which can lead to poisoning. Hair and blood can serve as biomarkers of lead exposure in the body. The purpose of this research is to determine the lead levels in the hair and blood of container truck drivers at PT. Trans Indo Sakti Surabaya using Atomic Absorption Spectrophotometry (AAS). This research is a descriptive study conducted at the Analytical Chemistry Laboratory of the Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya and the Standardization and Industrial Services Center Laboratory in Surabaya from October 2022 to May 2023. The samples used in the study are 15 container truck drivers, selected using purposive sampling technique. This study demonstrates results of the lowest hair lead level value at 0.1936 µg/g and the highest at 3.026 µg/g. Among the respondents, 9 were categorized as having normal levels, while 6 respondents were categorized as having abnormal levels. For the blood samples, the lowest lead level obtained was 0.146 µg/dL and the highest was 3.81 µg/dL. The lead levels are still categorized as normal. The presence of lead in the body is influenced by age, length of employment, duration of work, and smoking habits.
Lead Levels in the Urine of Active Smokers and Passive Smokers in the Surroundings of Wadung Asri Sidoarjo Juliana Christyaningsih; Qonita Salsabilla Amara Sherly Amri; Christ Kartika Rahayuningsih
International Journal of Advanced Health Science and Technology Vol. 3 No. 4 (2023): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i4.286

Abstract

An active smoker is someone who smokes cigarettes, resulting in an impact on their health, as well as on passive smokers and the surrounding environment. Smoking exposes individuals to the risk of lead inhalation due to cigarette smoke. Accumulated lead from smoked cigarettes can pose a health risk. This study aimed to identify respondent characteristics and analyze lead levels in both active and passive smokers. The research employed a descriptive approach with quantitative analysis. The sample consisted of urine samples from 20 active and passive smokers in the vicinity of Wadung Asri, selected using purposive sampling. The study was conducted at the Toxicology Laboratory, TLM Department of the Health Polytechnic in Surabaya, and at BARISTAND from October 2022 to May 2023. Based on the characteristics of active and passive smokers, regarding age, those aged 21-44 accounted for 25% of active smokers and 30% of passive smokers, those aged 45-59 constituted 10% in both categories, and those aged 60-74 constituted 15% of active smokers and 10% of passive smokers. The study results revealed that lead levels in the urine of active smokers ranged from a high of 0.1592 µg/mL to a low of 0.0885 µg/mL. Conversely, in passive smokers, lead levels in the urine ranged from a high of 0.0885 µg/mL to a low of 0.000032 µg/mL. Thus, the conclusion drawn from this study is that one respondent exhibited lead levels exceeding the normal threshold of <0.15 µg/mL.
Enhancing the Therapeutic Potential of Purslane Leaf Extract (Portulaca oleracea) for Cholesterol Management Juliana Christyaningsih; Fahin Izza; Regina Raiza; Azfa Alifatun Nayla; Deswita Aulia Pristanti; Faradyna Putri Az-Zahra; Mohd Nazil Salleh
International Journal of Advanced Health Science and Technology Vol. 4 No. 5 (2024): October
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v4i5.418

Abstract

Cholesterol is one of the essential molecules in humans. An imbalance in cholesterol levels in the body, such as high levels of triglycerides and low-density lipoprotein (LDL) or total cholesterol, can contribute to one of the leading causes of death in Indonesia. Diseases caused by high cholesterol levels include cardiovascular diseases, atherosclerosis, hypercholesterolemia, hyperlipidemia, coronary heart disease, hypertension, and stroke [6,9,14]. As a result, additional intake from sources like omega-3-rich supplements or herbal remedies is recommended. Historically, plant-based treatments have been used as pharmacological therapies. Researchers have explored the use of purslane (Portulaca oleracea) leaves, which contain unsaturated fatty acids and antioxidants, including omega-3 fatty acids, vitamins A and C, and minerals such as magnesium and potassium, offering cardiovascular benefits. The researchers utilized purslane extract as an herbal tea blend, serving as a natural alternative to supplements in capsule form, which may be more expensive. In extract form, purslane leaves are hygienically processed and mixed with herbal tea, creating a convenient health drink for daily consumption. The purslane leaf extract was obtained through method maceration using 96% food-grade ethanol. Proximate tests were conducted to assess total energy, energy from fat, ash content, moisture content, carbohydrates (by difference), total fat, and protein content [5,24]. Sensory evaluation was conducted by semi-trained panelists to assess the color, aroma, and taste of herbal tea blends containing purslane leaf extract. This evaluation aimed to collect subjective feedback from potential consumers regarding the organoleptic characteristics of the product.
Difference in quality of preparates of mice kidney histology using xylol and sunflower oil (Helianthus annuus) on deparafinization processes Adin Bhagawanti Poernomo; Juliana Christyaningsih; Ratno Tri Utomo; Wisnu Istanto
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.512

Abstract

ematoxylin-Eosin staining begins with deparaffinization. Deparaffinization aims to remove the remaining paraffin in the tissue and xylol is a commonly used deparaffinization material but the use of xylol has a bad impact on health and also the environment because of its toxic nature besides the price of xylol is relatively more expensive. A more natural material that is not harmful to the body and the environment is needed as an alternative to xylol. Sunflower oil is a type of vegetable oil that contains oleic acid. The oleic acid content in sunflower oil has a chain of carbon atoms that is non-polar and water insoluble so that it can dissolve paraffin which is non-polar and hydrophobic. This study was conducted at Faculty of Veterinary Medicine Airlangga University, Veterinary Farm Center and the Cytohistotechnology Laboratory of the Medical Laboratory Technology Department of the Surabaya Ministry of Health Polytechnic also this study was conducted from January - May 2025. which examined the differences in the quality of mice kidney histology preparations using xylol and sunflower oil in the deparaffinization process with experimental research methods and the sample size obtained using the federer formula was 24 preparations and divided into 4 groups including xylol, sunflower oil at 50oC for 5 minutes, 10 minutes and 15 minutes. The data were statistically processed with the Kruskal Wallis non-parametric test and the results showed that Ho was accepted (P = 0.350) so it can be concluded that there is no significant difference between the preparations deparafinitation with sunflower oil and xylol in hematoxylin eosin
Comparison Of Fixation Using NBF 10% and Honey 13% with Time Variation On Mice Heart Histology Preparations Using Hematoxylin Eosin Staining Amirah Putri Zerlina; Juliana Christyaningsih; Ratno Tri Utomo; Suliati Suliati
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.515

Abstract

The fixation stage is the initial stage of making histopathology preparations whose role is to maintain the cell structure of the tissue so that it is like living tissue and prevent tissue damage, thus facilitating the process of macroscopic and microscopic analysis. The fixative solution that is often used for standard preservation in anatomical pathology laboratory examinations is Neutral Buffered Formalin (NBF) 10%, which has the advantage of a pH that is close to neutral and effective in fixing soft tissue for 12-24 hours. However, NBF 10% is carcinogenic and has high toxicity. There is a natural fixative solution, honey, which contains fructose and glucose and has an acidic pH that has antimicrobial, antioxidant and antiautolysis properties. In addition, honey has also been widely used as a preservative because it is easily available, affordable, and without toxicity effects. The purpose of this study was to determine the comparison of the quality of histology preparations of mice heart tissue fixed with 10% NBF and 13% honey with time variations. This research is a type of laboratory experimental research conducted in November 2024-April 2025. The sample used is the heart of healthy male mice. The number of sample preparations used was 27 sample preparations. Data analysis through scoring and statistically processed using non-parametric Kruskal-Walis test, if there is a difference followed by Mann-Whitney test. The results showed that fixation with 13% honey solution with a time variation of 24 hours has the same comparison of preparation quality results with 10% NBF solution for 24 hours. Based on the Mann-Whitney test, the result is 0.018 (p < 0.05) for 13% honey solution for 12 hours and 0.634 (p > 0.05) for 13% honey solution for 24 hours. It can be concluded that fixation with 13% honey solution for 24 hours is effective as an alternative fixation solution with natural ingredients that are safe in fixing tissues
Potensi Mie yang Diperkaya dengan Kelor, Ikan Gabus, Okra, dan Singkong Modifikasi sebagai Pencegah Stunting: The Potential of Noodles Enriched with Moringa, Snakehead Fish, Okra, and Modified Cassava for Stunting Prevention Juliana Christyaningsih; Nur Hatijah; Laili Rahmawati
Amerta Nutrition Vol. 10 No. 2 (2026): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v10i2.2026.277-284

Abstract

Background: Pregnant women with chronic energy deficiency are at higher risk of delivering low birth weight and premature babies, increasing the likelihood of stunting by 1.66 times compared to those with normal nutritional status mothers. Therefore, nutrient-rich foods are essential to prevent stunting. Objectives: This study aims to analyze the nutritional content and sensory quality of noodles enriched with moringa leaves, snakehead fish, okra, and modified cassava. Methods: A laboratory experiment was conducted to determine the nutrient composition of each ingredient, and sensory evaluation was carried out by 25 semi-trained panelists from the Nutrition Study Program, Surabaya Health Polytechnic, Ministry of Health, Indonesia, using a 1–5 rating scale for aroma, color, taste, and texture. Results: The results showed that the highest protein content was found in snakehead fish flour (58.7%), fat in okra flour (21.48%), carbohydrates in modified cassava flour (88%), iron in moringa leaf flour (202 ppm), calcium in snakehead fish flour (3%), potassium in moringa leaf flour (0.84%), and sodium in snakehead fish flour (0.1%). The best sensory results were achieved with a noodle formulation of 83% wheat flour, 10% modified cassava flour, 2% okra flour, 2% moringa flour, and 3% snakehead fish flour. Conclusions: These enriched noodles demonstrated high nutritional value and good sensory acceptance.
Utilization of Teak Leaf Extract as a Substitute for Eosin in Hematoxylin Eosin Staining Agnes Desiana Paa; Agustina W. Djuma; Juliana Christyaningsih
Laboratory Journal of Infectious Diseases Vol. 2 No. 1 (2026): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hematoxylin–Eosin (HE) staining is widely used in histopathology to visualize tissue morphology. Eosin, a synthetic dye commonly used to stain cytoplasmic components, may cause environmental and health concerns, encouraging the search for safer natural alternatives. Young teak leaves (Tectona grandis) contain anthocyanin pigments with potential as natural staining agents. This study evaluated the effectiveness of teak leaf extract as a substitute for eosin in HE staining of uterine tissue. A quasi-experimental post-test only control group design was applied. Young teak leaves were extracted through maceration using 95% ethanol for seven days, followed by filtration and evaporation to obtain a concentrated extract. Uterine tissue preparations were stained using hematoxylin combined with 75% teak leaf extract, while eosin staining served as the control. Microscopic evaluation at 400x magnification was performed by an anatomical pathology specialist using a staining quality scoring system. The control group stained with eosin demonstrated optimal staining quality, with clear bluish-purple nuclei and bright pink cytoplasm, achieving a good score in all slides. In comparison, teak leaf extract produced pale purple nuclei and brownish-pink cytoplasm that remained distinguishable morphologically, though with lower staining quality. Overall, teak leaf extract demonstrated potential as a natural alternative to eosin, but further optimization is needed to improve staining performance.
The Counseling and Examination of Hypertension, Diabetes, and Uric Acid for the Elderly of Bulak Surabaya Village Through Healthy Education: Indonesia Liliek Soetjiatie; Bedjo Utomo; Juliana Christyaningsih; Taufiqurahman Taufiqurahman
Jurnal Mandala Pengabdian Masyarakat Vol. 7 No. 1 (2026): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpm.v7i1.788

Abstract

Lansia memiliki peran penting dalam masyarakat, terutama sebagai teladan dan panutan generasi muda, sehingga membutuhkan perhatian khusus untuk menjaga kualitas hidup yang optimal, khususnya di bidang kesehatan. Permasalahan utama yang dihadapi adalah meningkatnya prevalensi penyakit tidak menular, seperti hipertensi, diabetes mellitus, dan asam urat, yang berdampak pada kesehatan fisik maupun mental lansia serta menurunkan kualitas hidup. Mitra dalam program pengabdian kepada masyarakat ini adalah Kelurahan Bulak, wilayah terbesar kedua di area kerja Puskesmas Kenjeran, dengan dukungan layanan kesehatan berupa puskesmas pembantu dan posyandu lansia. Terdapat tujuh posyandu lansia di Kelurahan Bulak dengan 48 peserta aktif di masing-masing posyandu, sehingga setiap bulan rata-rata 336 lansia mendapatkan pelayanan, meskipun keterbatasan fasilitas menjadi kendala. Tujuan kegiatan ini adalah meningkatkan pemahaman dan kesadaran lansia mengenai pentingnya menjaga kesehatan, disiplin mengonsumsi obat, melakukan pemeriksaan rutin, serta menerapkan pola hidup sehat. Metode yang digunakan meliputi penyuluhan dan seminar tentang penyakit tidak menular, edukasi mengenai makanan sehat bagi lansia dengan penyakit tidak menular, pendampingan dan pelatihan pengolahan makanan sehat, pemeriksaan kesehatan dengan pengukuran tiga parameter laboratorium yaitu gula darah, kolesterol, dan asam urat, serta pembinaan perilaku hidup sehat melalui senam lansia, edukasi tidak merokok, dan menjaga suasana hati. Hasil kegiatan menunjukkan peningkatan pemahaman lansia terhadap bahaya penyakit tidak menular, kesadaran untuk tertib minum obat, pemeriksaan kesehatan teratur, serta praktik hidup sehat yang lebih baik. Kesimpulan dari program ini adalah pendampingan dan edukasi mampu meningkatkan kualitas hidup lansia, menjadikan mereka tetap berdaya, bernilai, dan bahagia pada usia lanjut, serta berkontribusi positif bagi keluarga dan masyarakat.
Descriptive Study: Nutrition Knowledge, Physical Activity, and Nutritional Status of 11th-Grade Students at SMA Muhammadiyah 2 Surabaya Amira Salsabil; Nuning Marina Pengge; Juliana Christyaningsih; Riezky Faisal Nugroho
Journal of Nutrition Explorations Vol. 3 No. 4 (2025): November
Publisher : Politeknik Kesehatan Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/jone.v3i4.152

Abstract

Adolescence is a critical period of rapid growth and behavioral change that influences nutritional needs and lifestyle choices. This study aims to describe the nutrition knowledge, physical activity levels, and nutritional status of 11th-grade students at SMA Muhammadiyah 2 Surabaya. A descriptive study was conducted involving 58 students selected through proportional random sampling. Data were collected using validated questionnaires on nutrition knowledge and physical activity, as well as anthropometric measurements. Nutritional status was assessed using BMI-for-age and categorized based on WHO z-score standards. Data were analyzed using SPSS with univariate and bivariate methods. The majority of respondents were female (67.2%) and aged 16 (65.5%). Most had high nutrition knowledge (70.7%), engaged in light physical activity (74.1%), and had normal nutritional status (60.3%). It is recommended that schools enhance regular nutrition education, promote physical activity, and monitor students' nutritional status to support healthy adolescent development.
The Relationship Levels of Consumption and Physical Activity with Nutritional Status of Staff and Personnel Addendant General of Military Resort (AJENREM) Type B 081 Madiun Dian Putri Agustina; Annas Buanasita; Inne Soesanti; Juliana Christyaningsih
Journal of Nutrition Explorations Vol. 1 No. 2 (2023): May
Publisher : Politeknik Kesehatan Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/jone.v1i2.159

Abstract

Nutritional status is closely related to health. Nutrition plays an important role in the body as a provider of energy, maintaining body tissues and regulating metabolic processes in the body, including for military personnel who are required to have optimal physical conditions. This study aims to determine the relationship between the level of consumption and physical activity with the nutritional status of the staff and personnel of AJENREM Type B 081 Madiun. This study used a cross-sectional design with a sample of 43 respondents, which was conducted using a simple random sampling technique. Data were collected by measuring weight, height, filling out the IPAQ-Short questionnaire and interviewing food recall 2x24 hours. Relationship analysis was carried out using the Spearman Rho test. The results showed that there was no relationship between the level of energy consumption, protein, fat and carbohydrates with nutritional status. There was a significant relationship between physical activity (p=0.000) and the nutritional status of the staff and personnel of AJENREM Type B 081 Madiun. Therefore, more physical activity is recommended in maintain the optimal nutritional status.
Co-Authors ., Pestariati Adin Bhagawanti Poernomo Adistya Dwi Cantika Agnes Desiana Paa Agustina Welhelmina Djuma Ahmad Yudianto Ainur Rifda Kurnia Puspita Sari Ainur Wachidah Choirun Niza Aisya Eliarosa Ajeng Kinanti Anggraini Amiliya Antika Dewi Amira Salsabil Amirah Putri Zerlina Andriyani, Nurul Ani Intiyati Ani Media Harumi Anita Dwi Anggraini Annas Buanasita Ashalya Eka Astuti Setiyani, Astuti Ayu Puspitasari Ayunis Naba'ur Rhodiyah Azfa Alifatun Nayla Bambang Hadi Sugito Bedjo Utomo Binti Yunariyah, Binti Briansky, Sabira Alvayogi Christ Kartika Rahayuningsih Darjati Deddy Adam Dedi Kurniawan Demes Nurmayanti Deswita Aulia Pristanti Devi Eka Ratnasari Devi Puspitasari Diah Titik Mutiarawati Dian Putri Agustina Dian Rahma Sania Dian Shofiya Dian Shofiya Dwi Purwanti Dyah Purbosari Dwi Pitaloka Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hani Endarini, Lully Hanni Endro Yulianto Ersanto, Novi Ervi Husni, Ervi Evi Yunita N Evy Diah Woelansari Fahin Izza Faradyna Putri Az-Zahra Ferry Kriswandana Fika Nur Septianingtias Filisia Luna Andyana Firda Amalia Pratiwi Ginarsih, Yuni Hadi Suryono Halimah, Husnul Handayati, Anik HANIFA RAHMAWATI Haqqi, Faishol Harianto Notopuro Hilmi Yumni Hindaryani, Nurul Husnul Halimah I Dewa Gede Hari Wisana Ida Ariyanti, Ida Imam Sarwo Indah Lestari Indah Lestari Indah Lestari, Indah Inne Soesanti Ira Puspita Sari Ira Puspitasari Ira Puspitasari Ira Rahayu Tiyar Sari Irwan Sulistio Isfentiani, Dina Islaha, Maria Isnaini, Dian Isnanto Istanto, Wisnu Jayeng Wijayanti, Erlyna Karina, Lidya Karina, Lidya Kartika Rahayuningsih, Christ Khambali, Khambali Khristine Saputri Kiaonarn Ongko Waluyo Kiaonarni OW Kristiningsih, Elly Kuswanto, Muhammad Laili Rahmawati Laili Rahmawati Laili Rahmawati, Laili Lembunai Tat Alberta, Lembunai Tat Lieliek Soetjiatie Liliek Soetjiatie Liliek Soetjiatie Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Mairo, Queen Khoirun Nisa’ Makruf, Anang Mamik Mamik Mamik Mamik Marina Pengge Maulidyah Aisyah Putri Nadifah Meidayana, Ema Meisya Argia Sisviani Deva Melania Rizerda Pebianti Melina Sari Melina Sari STP, M.Si MINARTI Minarti Minarti Minarti Minarti Mohd Nazil Salleh Muhammad Andy Firmansyah Mujayanto Mujayanto Muna Setia Madihah Museyaroh, Museyaroh Mutiarawati, Diah Titik N. S. Widodo Nadia Salsabila Nastiti, Aliffah Nurria Novita Machfiratul Jannah Novita, Devy Nugroho, Riezky Faisal Nuning Nuning Marina Pengge Nur Hatijah Nur Hatijah Nur Hatijah Nur Hatijah Nur Hatijah Nur Hatijah Nur Kholifah, Siti Nuswantari, Atika Oktavianingsih, Yulis Patcharanee Pavadhgul Pebianti, Melania Rizerda Pengge, Nuning Marina Pojor Srirejeki Eka Lestari Purwanti, Dwi Puspita Dewi, Teresia Retna Puspita Zella Wigati Puspitasari, Erlita Putri Aniq Oktavia Qonita Salsabilla Amara Sherly Amri Ratna Sari Ratno Tri Utomo Regina Raiza Retna Puspitadewi, Teresia Retno Puspitadewi, Teresia Retno Sasongko Wati Retno Sasongkowati Rihlah Sa'idah Rika Yulia Rokhmalia, Fitri Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Sherly Jeniawaty, Sherly Sholikah, Mar'atus Siagian, Hotmaida Siti Alfiah Siti Mar'atus Slamet Wardoyo Sri Utami SRI UTAMI Sugihani Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Suliati Suliati Sulistyowati, Dwi Wahyu Wulan Sumasto, Hery Supriyatin, Endah Suyanto, Beny Tatarini Ika Pipitcahyani Taufiqurahman Taufiqurahman Taufiqurrahman Taufiqurrahman Taufiqurrahman Taufiqurrahman Tjipto Rini Toni Watoyani Trisnania, Iid Dani Ulfa Hidayah Umul Annisa Fadhila Utomo, Ratno Tri Vernanda Arsya Nabilla Veronica, . Veronica, . Wahyurianto, Yasin Win Darmanto Wisnu Istanto Wulandari, Meisa Yusianti Silviani Zida Shabrina Akmalia