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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Primatologi Indonesia Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Microbiology Indonesia Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Penelitian Hasil Hutan Jurnal Teknologi Pangan agriTECH JURNAL BIOLOGI INDONESIA The Indonesian Biomedical Journal Jurnal Aplikasi Teknologi Pangan JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Psikologi Sosial Jurnal Respirologi Indonesia JURNAL KAJIAN VETERINER ZOOTEC Makara Journal of Science The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Kedokteran Hewan Jurnal Penelitian Hasil Hutan

Dondin Sajuthi

Departemen Klinik, Reproduksi, Dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jl. Raya Dramaga, Babakan, Dramaga, Bogor, Jawa Barat, Indonesia 16680

Author-ID : 255503

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Macaca nemestrina and Dengue Virus Infectivity: a Potential Model for Evaluating Dengue Vaccine Candidates SUSANA WIDJAJA; IMELDA WINOTO; JONATHAN STURGIS; CHAIRIN N MAROEF; ERLIN LISTIYANINGSIH; RATNA TAN; JOKO PAMUNGKAS; PATRICK J BLAIR; DONDIN SAJUTHI; KEVIN RANDALL PORTER
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Macaca nemestrina has been shown to respond to infectious disease agents, such as HIV, and is more sensitive compared to other species of macaques such as rhesus (M. mulatta) and cynomolgus monkeys (M. fascicularis). To evaluate M. nemestrina for the ability to support dengue (DEN) viremia and serve potentially as an improved model for testing DEN vaccines, a series of experiments were conducted using primary viral isolates from individuals with DEN virus infections. This study shows that M. nemestrina develops consistent, measurable viremia with all four DEN serotypes and produces immune responses sufficient to protect against homologous virus. Anti-dengue antibodies generated after infection are predominately IgG1. This species of monkey therefore appears to be a suitable model for testing DEN virus vaccine candidates.

Isolation and Characterization of Simian Retrovirus Type D from Macaca fascicularis and M. nemestrina in Indonesia DIAH ISKANDRIATI; UUS SAEPULOH; SILMI MARIYA; RICHARD F GRANT; DEDY DURYADI SOLIHIN; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (AIDS) in Asian macaques. In the past, SRV isolates from macaques had only been identified at the US primate centers, outside the country of origin and after the animals had been introduced into a new environment. In this study, we report the first isolation, cultivation and molecular characterization of the type D simian retrovirus naturally infecting wild caught macaques in their natural habitats in the country of origin, in this case, Indonesia. When peripheral blood mononuclear cells (PBMC) from Macaca fascicularis (Mf) and M. nemestrina (Mn) were co-cultured with Raji human B-cell line, syncytia were observed microscopically and confirmed by immunofluoresence assay using antibody to SRV-2. Immunoblot analysis of purified Mf-ET1006 from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV. Sequence analysis of Mf isolates in the viral envelope region revealed high homology to SRV-2 (94-96%). On the other hand, the homologies in the envelope region of Mn isolates were less than 80% to SRV-1, SRV-2, SRV-3 and Mf isolates. This study suggests that the isolate from Mn may be different from any other published SRV isolates.

Dissemination in Pigtailed Macaques after Primary Infection of Dengue-3 Virus JOKO PAMUNGKAS; DIAH ISKANDRIATI; UUS SAEPULOH; MOSES AFFANDI; ESTHER ARIFIN; YASMINA PARAMASTRI; FITRIYA NUR ANISA DEWI; DONDIN SAJUTHI
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Nonhuman primates (NHPs) play as indispensable animal model in biomedical research for studying a variety of human health issues, diseases and disorders, therapies, and preventive strategies. Since the immunological and physiological responses of NHPs, at some extent, to experimental viral infections are similar to humans, it is possible that studies of dengue infection in NHPs may aid understanding of dengue infection in humans. In this study,we used pigtailed macaques (Macaca nemestrina) as the experimental animal to study dengue-3 (DEN-3) virus infection.We evaluated DEN-3 viral distribution and replication sites after a primary infection in all collected tissues. Sequential localization in tissue of DEN-3 virus was studied in pigtailed macaques euthanized three days post viral inoculation (10 pfu mL ). Pigtailed macaque that was inoculated subcutaneously or intravenously; showed the highest viremia (62.94 pfu mL and 58.62 pfu mL ) detected by one step reverse transcription real time PCR. The virus inoculated in pigtailed macaques by subcutaneous injection was rapidly disseminated from the inoculation site to the lymph nodes, adrenal glands, kidneys, heart, thyroid, liver, prostate gland, and seminal vesicles. Meanwhile, dissemination of dengue virus in pigtailed macaques inoculated intravenously was detected in lymph nodes, thymus, salivary glands, liver, and prostate gland. This study suggested that the above mentioned-tissue specimens are involved or affected by DEN-3 virus replication and the route of infection seemed to have influenced the virus dissemination.

Cloning and Expression of Serotype-2 Simian Betaretrovirus Reverse Transcriptase Gene Isolated from Indonesian Cynomolgus Monkey in Escherichia coli UUS SAEPULOH; DIAH ISKANDRIATI; FUNGKEY HOETAMA; SELA SEPTIMA MARIYA; DEDY DURYADI SOLIHIN; JOKO PAMUNGKAS; DONDIN SAJUTHI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

In this study, we isolated the simian betaretrovirus serotype-2 (SRV-2) reverse transcriptase (RT) gene from infected Indonesian cynomolgus monkey (Macaca fascicularis). The gene was then cloned in Escherichia coli expression system. The SRV-2 RT gene is located between nucleotides 3284-4925 in the polyprotein (Pol) region encodes 547 amino acids. Analysis of expression using SDS-PAGE and western blot techniques showed a specific band of 64.9 kDa, indicating SRV-2 RT recombinant enzyme. Purification of SRV-2 RT recombinant enzyme produced 312 μg mL-1 protein with 7.1 U μL-1 enzyme activities. Application of this recombinant enzyme in reverse transcription-PCR (RT-PCR) of β-globin and β-actin genes produced DNA fragments of 206 and 350 bp, indicating amplification of β-globin and β-actin genes, respectively. Therefore, the expressed SRV-2 RT enzyme was proven to be functional, although the activity was low.

Primary Tupaia javanica Hepatocytes Cultures As In Vitro Replication System for Ape Hepatitis B Viruses MARYATI SURYA; DIAH ISKANDRIATI; SILMI MARIYA; UUS SAEPULOH; PERMANAWATI PERMANAWATI; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Hepatitis B virus (HBV) is a DNA virus with liver as primary target organ.This virus caused chronic infection that can progress to cirrhosis, liver cancer and even death. In vitro model system of hepatocyte cultures is important and widely used to study a variety aspects of hepatitis B. Development of small animal Tupaia sp. for the in vitro model system is an alternative to the existinghepatocyte cultures. The specific purpose of the studyis to develop Tupaia javanica hepatocytes culture for HBV replication, and in a broader spectrum to answer the need for in vitro model of hepatocytes. Primary T. javanica hepatocytes (PTH) culture was successfully maintained for 14 days to reach 80% confluence, and infection of Javan gibbon HBV (GiHBV) and orangutan HBV (OuHBV) onto the culture on day 15 showed viral replication for up to eight days as measured by polymerase chain reaction (PCR). PCR quantification indicated that the highest copy number of DNA virus was detected onday two anddecreased until day 8 after infection. Cell receptor for HBV attachment, known as sodium taurocholate cotransporting polypeptide was expressed on the surface of PTH and shown as green luminenscent when observed by immunofluorescence assay. Sequence of partialS gene from the apes HBVs after the viruses have been infected to the PTH showed amino acid identity to their wildtype as high as 99.29% for GiHBV and 95.71% for OuHBV. This study suggested that the PTH can support the replication of GiHBV andÂ OuHBV.Â

Co-Authors . Permanawati Achmad - Taher Achmad Taher Achmad Taher Achmad, Taher Adi Winarto Agus Harsoyo Agus Harsoyo Agus Lelana Agus Wijaya Agus Wijaya Ahmad Kamal Nasution Alexander Nater Anita Esfandiari Annisa Dewi, Fitriya Nur Antonius Suwanto Anwar Wardy Warongan Arief Boediono Aryo Tedjo Bambang Purwantara Bayu Febram Prasetyo Bayu Rosadi Budiman Bela C Hanny Wijaya C. Hany Wijaya Cecep Kusmana CHAIRIN N MAROEF Chusnul Choliq Chusnul Choliq Chusnul Choliq Cucu K Sajuthi Dadang Makmun Daldiyono Daldiyono Daldiyono Daldiyono Dedi Rahmat Setiadi DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa DIAH ISKANDRIATI Diah Iskandriati DIAH ISKANDRIATI Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati Douglas M. Bowden Dwi Atmoko Agung Nugroho DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Entang Iskandar Entang Iskandar Entang Iskandar Erdiansyah Rahmi ERIC HAYES Eric Hayes Eric Hayes ERLIN LISTIYANINGSIH Erni Sulistiawati ESTHER ARIFIN Fauzi Abdurrahman Munggaran Fhady Risckhy Loe Fransiska R Zakaria FUNGKEY HOETAMA Gede Eko Darmono Gregory K. Wilkerson Hadi S Alikodra Hajrawati Hajrawati Harrold Halilintar MR Subu Taopan Henny Nuraini HERA MAHESHWARI Huda S Darusman Huda Shalahuddin Darusman Huda Shalahudin Darusman I Gusti Agung Arta Putra I Gusti Ngurah Sudisma I Ketut Sutama I NENGAH BUDIARSA I Nengah Budiarsa I Nengah Budiarsa I wayan Teguh Wibawan IETJE WIENTARSIH Iis Afriantini Iman Rusmana Iman Supriatna IMELDA WINOTO Indra Bachtiar Indra Bachtiar Irawan Jusuf Irma H Suparto Irma H Suparto Irma H Suparto Irma Herawati Suparto Irma Isnafia Arief Irma Suparto J. Mark Cline Jansen Manansang Jansen Manansang Jayusman Arsiyanti Joesoef JONATHAN STURGIS Kanti Rahmi Fauziyah Kartika Sari KATHARINA JESSICA KEVIN RANDALL PORTER Koeswinarning Sigit Latifah K. Darusman Latifah K. Darusman Leni Maylina Lina Elfita Lina Elfita Luluk Lailatul Hasanah LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji M Agus Setiadi MARYATI SURYA Maxs Urias Ebenheizer Sanam Michael Krützen MOSES AFFANDI Mozes R. Tolihere Muchidin Noordin MUHAMMAD AGIL Nanik Hidayatik Nur Hidayati Nurhidayat . Nurul Oktaviani Oktaviani, Nurul Oriza Savitri Ariantie Paini Sri Widyati Pamungkas, Joko PATRICK J BLAIR Peni Harjosworo PENI SUPRAPTI HARDJOSWORO Permanawati Permanawati Permanawati Permanawati PERMANAWATI PERMANAWATI PERMANAWATI, PERMANAWATI Pijoh, Deyv Praira, Willy Pudji Astuti Pudji Astuti PUDJI ASTUTI Puji Rianti Puspita Sari Putri, Zulfitra Utami R. Iis Arifiantini R.P Agus Lelana RATNA TAN Retno Wulansari Reviany Widjajakusuma Reviany Widjajakusuma Ria Oktarina RICHARD F GRANT Rini Widayanti Rumlaklak, Yanse Yane Sajuthi, Cucu K Sela S Mariya Sela S. Mariya SELA SEPTIMA MARIYA Sela Septima Mariya Septi Iriani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Setyo Widi Nugroho Setyo Widodo SILMI MARIYA Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya SILMI MARIYA Silvia Triwidyaningtyas Sony Hartono Wijaya Sri Rahmatul Laila Sri Soepraptini Mansjoer Sri Supraptini Mansjoer Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suharyanto Suharyanto Suharyanto Suharyanto Sulistiyani Sulistiyani . SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Suparto, Irma Sus Derthi Widhyai Sus Derthi Widhyari SUSAN SOKA SUSANA WIDJAJA Suzy Tomongo Taher, Achmad TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Ari Prabowo Teguh Budipitojo Tejasari . Thomas Mata Hine Tiara Widyaputri Tri Wahyu Pangestiningsih Trini i Susmiat Trini Susmiati Tuti Suryati Tuty L Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Unang Supratman Uus Saefullah Uus Saepuloh Uus Saepuloh Uus Saepuloh Uus Saepuloh UUS SAEPULOH UUS SAEPULOH Vetnizah Juniantito Villiandra Villiandra Villiandra Villiandra Villiandra Villiandra Vincentia Trisna Yoelinda Willy Praira Yanse Yanne Rumlaklak Yanti . Yanti Yanti Yasmina A Pramastri Yasmina A. Paramastri Yasmina Arditi Pramastri YASMINA PARAMASTRI Yoga Yuniadi Yohana Tri Hastuti Yohana Tri Hastuti Yohana Tri Hastuti Yuli Purwandari Kristiangingrum Yundari, Yundari Zulfitra Utami Putri Zulfitra Utami Putri Zuraida Zuraida Zuraida

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Journal : Microbiology Indonesia