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1. Immunohistochemical Study of the Immunoreactive Follicle Stimulating Hormone (ir-FSH) Cells Distribution in Pituitary Gland of Rat(Rattus norvegicus) Patrick Flaggellata; Sri Wahyuni; Muhammad Jalaluddin; Hamny Hamny; Gholib Gholib; T. Armansyah, TR; Muslim Akmal
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4092

Abstract

The objective of this study was to identified the development of the immunoreactive follicle stimulating hormone (ir-FSH) cells in pituitary glands of rats (Rattus norvegicus)in different age using immunohistochemical (IHC) methods. The pituitary organ that used in this study was collected from eight white female rats aged 2, 4, 6, and 12 months. Pituitary glands were processed histologically and stained with IHC methods to detect the ir-FSH cells. The result showed that the ir-FSH cells were observed in pars distalis (PD), pars tuberalis (PT), and pars intermedia (PI) of adenohypophysis with different number of cells. Furthermore, ir-FSH cells were found abundantly (+++) in the PT and a few (+) in PD of rats aged two months. In rats aged 4 months those cells were distributed in slight number (+) in PT and abundant (+++) in PD. In addition, the cells were also found in a great quantities in rats whose ages were 6 months, (+++) in both PT and PD, but these cells decreased (++) in rats aged 12 months in PD and absent (-) in PT. In conclusion, the devalopment and distribution of ir-FSH cells were found in pars tuberalis and pars distalis adenohypophysis in the pituitary glands of rats aged 2 up to 12 months with different number and distribution patterns.
3. An Immunohistochemical Study of Alpha Estrogen Receptor (ERα) Development in Ovary and Uterus of Rat (Rattus norvegicus) Sri Wahyuni; Chintya Desfariza; Hamny Hamny; Muslim Akmal; Gholib Gholib; T. Armansyah
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4100

Abstract

This research aimed to observe and identify the development of estrogen receptor alpha (ERα) in the ovary and uterus of rats (Rattus norvegicus) at various age levels using immunohistochemistry (IHC). The ovary and uterus were collected from eight female rats aged 2, 4, 6, and 12 months and processed histologically and stained with IHC staining. This research showed, the intensity of ERα expression was found in high abundance (+++) in stroma and the corpus luteum and was weakly found (+) in the theca cells, blood vessels, oocytes and germinal epithelium of rats aged 2 months. Additionally, ERα expression in rats aged 4 months had similarities with rats aged 2 months, but the intensity of ERα had increased (++) in ovarian theca cells, blood vessels, and oocytes. In ovaries of rats aged 6 and 12 months, ERα expression had decreased (++) in the stroma and the corpus luteum and had a low expression (+) in the blood vessel and oocytes. The uterus of rats aged 2 months had low expression of ERα (+) in the stroma and luminal epithelium, moderate (++) in the myometrium, but was not found in the uterine glands. Furthermore, the expression in the uterus of rats at the age of 4 months had increased in the stroma and luminal epithelium (++) and uterine glands (+).  At the age of 6 months, it found excessively (+++) in the stroma, myometrium, and luminal epithelium, moderate expression (++) in the uterine glands.  The expression of ERα in uterus aged 12 months was almost equal to the uterus of rats aged 6 months, but the expression in the myometrium and epithelial luminal had decreased (++). It can be concluded that the development and distribution pattern of ERα in the ovaries and uterus of rats are different at each age levels, it may be related to the growth and development of reproductive organs and also closely related to the estrus cycles of rats.
21. KAJIAN HISTOKIMIA SEBARAN KARBOHIDRAT PADA KELENJAR MANDIBULARIS DAN KELENJAR LINGUALIS AYAM PETELUR (Gallus sp.) Histochemical Study of Mandibular and Lingual Glands Carbohydrate Distribution in Layer (Gallus sp.) Hamny H
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i2.4622

Abstract

The objective of this study was to investigate the distribution of carbohydrate in the mandibular and lingual glands of layer in difference age groups histochemically.The study was observed the mandibular and lingual glands from nine layers which grouped into three groups namely starter aged 40 days old, productive aged seven months old, and post-productive aged 1.5 years old. Each group consisted of three layers. A small piece of mandibular and lingual glands were fixed in Bouin solution for subsequent processed into histological preparation.The distribution of carbohydrate in both of glands detected by alcian blue staining (AB) pH 2.5 and periodic acid schiff (PAS). The result showed that mandibular and lingual glands of the three group layers reacted positively to AB pH 2.5 and PAS which found in the cytoplasm of cell and in the secretion on lumen. In the cytoplasm, the mandibular and lingualis reacted positively to AB pH 2.5 and PAS with the intensity ranging from weak reaction(+) to strong (+++), while in the lumen of glands showed negative reaction (-) to positive with the intensity of weak (+) to strong (+++). In mandibular gland, the acid carbohydrates were found with strong intensity (+++) in the productive and post-productive layer, while neutral carbohydrates were found with the same intensity on starter, productive and post-productive layers. In the lingual gland, the acid carbohydrates found with strong intensity in the three groups of layers while the neutral were found with strong intensity (+++) in the starter and post-productive layer. Positive reaction to AB pH 2.5 and PAS indicated that both of the salivary glands contain the neutral and acid carbohydrate. ____________________________________________________________________________________________________________________ Key words: mandibular gland, lingual gland, acid carbohydrate, neutral carbohydrate, layer
RASIO KETEBALAN DINDING TERHADAP DIAMETER TULANG HUMERUS AYAM KAMPUNG (Gallus domesticus) DAN BURUNG MERPATI (Columba domestica) Idawati Nasution; Shinta Mutia RM; Hamny -
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2264

Abstract

Penelitian ini bertujuan mengetahui perbandingan ketebalan dinding terhadap diameter tulang humerus pada ayam kampung (Gallus domesticus) dan burung merpati (Columba domestica). Dua puluh tulang humerus yang berasal dari 10 ekor burung merpati jantan dipisahkan dari jaringan di sekitarnya, kemudian tulang humerus tersebut direndam dalam formalin 5% dan dipotong secara melintang dengan menggunakan gergaji tulang. Ketebalan dinding terhadap tulang humerus diukur dengan menggunakan jangka sorong. Hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa perbandingan ketebalan dinding dan diameter tulang humerus pada ayam kampung adalah 1 : 4,94 sedangkan pada burung merpati adalah 1 : 5,80. Tulang humerus ayam kampung lebih tebal dari pada burung merpati jika dibandingkan diameter tulang dari masing-masing unggas tersebut.Kata kunci: Gallus domesticus, Columba domestica, tulang humerus, rasio, diameter, ketebalan
The Comparison of The Death Time of Hanged and Unhanged Broilers After Slaughter Azhari Azhari; Sugito Sugito; Ferllya Adela Putri; Wahyu Eka Sari; M. Jalaluddin; Hamny Sofyan; Muttaqien Muttaqien; Rosmaidar Rosmaidar; M. Isa
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.33141

Abstract

Halal slaughter is a way to kill animals by the process of draining blood properly, perfectly, and according to the sharia. One of the critical points of halal in the slaughtering process is the timeof death of the broilers after slaughter. The objective of this study was to determine the difference between the time of death of hanged broilers and unhanged broilers, in order to find out the right time before the scalding. The samples used were 10 male broilers (Gallus domesticus) five weeks old with body weight 1.6-1.7 kg/head. The variable observed was the time of death of hangedand unhanged broilers after slaughter looking at the signs of  death,  such  as  heart  rate  stopped,  pupil or  eyelid  reflexes,  and  stopped breathing. This study used a completely randomized design (RAL) with two treatments, such as slaughter of chickens in a hanging position (P1) and chickens without hanging (P2). The test data were analyzed using the independent T-test SPSS 25. The results obtained that the average time of death of hanged broilers after slaughter was 82,80 seconds, while the average time of death of unhanged broilers was 162.60 seconds. The test results of the two treatments with a significant level of 0.05 showed a significant difference. The conclusion of this study was time of death of hanged broilers is faster than that of unhanged broilers after slaughter
TOTAL BAKTERI PSIKOTROPIK IKAN NILA (Oreochromis niloticus) YANG DIBERI PENINGKATAN SUHU PADA SAAT PEMELIHARAAN (Total of Phsycotrophic Bacteria of Nile Tilapia (Oreochromis niloticus) Reared in High Water Temperature) Safni Kamal; Nurliana Nurliana; Faisal Jamin; Sulasmi Sulasmi; Hamny Hamny; Fakhrurrazi Fakhrurrazi
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4035

Abstract

This research aimed to calculate the number of phsycotrophic bacteria of nile tilapia reared in increased water temperature. For this purpose, 12 nile tilapias with body weight of 35-40 g were randomly allotted into 2 treatments groups with 3 replications. Fish in group 1 (P1) was reared in normal temperature (29±1° C) and group II (P2) was reared in high water temperature (35±1 C). All fish were fed commercial diet 3 times a day for 21 consecutive days. T-test analysis revealed a significant decrease in number of psychotropic bacteria (P0.01) in group P2. Total plate count (TPC) resulted in fish gills, intestinal, and muscle were 1.5×10³, 7.7x10³, and 9.7x10³ cfu/g respectively. In conclusion, increasing of water temperature reduce the number of phsycotrophic bacteria of nile tilapia.
The Pattern of Carbohydrate Distribution of Accessory Sex Glands in Male Muntjac (Muntiacus muntjak muntjak) Sri Wahyuni; Boni Anggara; Muslim Akmal; Hamny Hamny; Mustafa Sabri
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i2.3355

Abstract

The objective of this study was to investigate the pattern of carbohydrate distribution of accressory sex glands in male muntjac (Muntiacus muntjak muntjak) in the velvet antler stage. In macroanatomy observation, muntjac accessory sex glands consist of the ampulla, vesicular gland, pars diseminate prostat, and bulbourethral gland. The observation of neutral carbohydrate distribution has done by periodic acid Schiff (PAS) staining. In addition, acid carbohydrate was observed by using alcian blue (AB) pH2,5 staining. Result of this research showed that neutral and acid carbohydrate scattering in each accessories sex gland with different pattern. Neutral carbohydrate with medium (++) and high (+++) intensity reaction was found in the ampulla, vesicularis gland, and bulbourethral gland and strong in prostat gland. The acid carbohydrate with low (+) and medium (++) intensity reaction was observed in the ampulla, low in vesicularis gland, and negative in prostat and bulbourethral glands. In conclusion, the neutral carbohydrate was found dominantly on surface of all accessory sex glands of male muntjac in velvet antler stage with highest concentration and intensity of colour in prostat gland.Key words: carbohydrate, accessory sex glands, muntjac (Muntiacus muntjak muntjak)
19. Histological of Thymus in Tegal Duck (Anas javanicus) at Different Ages Hamdani Budiman; Anugrah Septian; Hamny Hamny; Zainuddin Zainuddin; Cut Dahlia Iskandar; Mustafa Sabri; Muhammad Hambal; Abdul Harris
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.3523

Abstract

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages. The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks.  The distribution of Hassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer.
5. Identification and Distribution of Carbohydrates on Sex Accessories Glands of Male Aceh Cattle Muhammad Jalaluddin; Mustafa Sabri; Herlina Yuliani; Fadli A. Gani; Hamny Hamny; Sri Wahyuni
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4097

Abstract

This study aimed to determine the content and distribution of carbohydrate in the sex accessory glands of Aceh cattle. The sex accessory glands of Aceh cattle that were used consisted of a vesicular gland, prostate gland and bulbourethral gland. The entire gland was processed into histological preparation. The content of neutral carbohydrate distribution was observed with periodic acid Schiff (PAS) staining while acid carbohydrates using alcian blue (AB) staining pH 2,5. The results showed that the content and distribution patterns of carbohydrate acid and neutral carbohydrate were different on every sex accessory glands of Aceh cattle. The content of acid-carbohydrate with the intensity of reaction with moderate (++) to strong (+++) intensity was found on the prostate gland and bulbourethral gland. Meanwhile, the neutral carbohydrate with low intensity (+) was found in the vesicular gland, prostate gland and bulbourethral gland. The content of acid carbohydrate was scattered throughout the prostate gland and bulbourethral gland of Aceh cattle, a carbohydrate with high concentrations (+++) was found in the bulbourethral gland.
31. Histological of Tractus Digestivus of Domestical Catfish (Clarias batracus) Aliman Irfandi; Cut Dahlia Iskandar; Zainuddin Zainuddin; Dian Masyitha; Fitriani Fitriani; Hamny Hamny; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3535

Abstract

Domestical catfish is grouped into omnivora, although the fish is tended into carnivora.  The aim of the present study was to investigate histology of alimentary tract of domestical catfish (Clarias batrachus). In order to determine alimentary tract of domestical catfish, oesophagus, gastric and intestines is investigated by Haematoxilin Eosin. Histology exploration was performed each layer of alimentary tract’s wall consist of lamina epithelia, propria, and muscularis mucosa. Collagen connective tissue was developed tunica submucosa. The muscle of tunica muscularis was performed circular and elongated. Dense connective tissue and blood vessels was located in the tunica serosa. Histology exploration revealed has stratified squamous epithelium, mucosit cells, eosinophilic club cells on the lamina epithelia, and taste buds on the lamina propria in oesophagus. Gastric was establised simple prismatic cells, and glands of gastric on the lamina propria. Furthermore intestine was showed villi and goblet cells. There were not showed Brunner and Liberkhun gland. In conclusion, histology of alimentary tract was revealed from tunica mucosa, submucosa, and serosa.
Co-Authors . Nurliana . Zuchri ., Daniel Abdul Harris Abdullah Hamzah Adi Purnawarman, Adi Ahmad Azhari Nopiosi Alfajri Saputra Alfajri Saputra Ali, Ihfan Khaidir Aliman Irfandi Amalia Sutriana Amiruddin A Andi Novita Anugrah Septian Aris Junaidi Ayu Agita Ginting Azhari Azhari Bambang Pontjo Priosoeryanto Boni Anggara Budianto Panjaitan Budianto Panjaitan Chairun Nisa Chika Nahara Ramadhanty Chintya Desfariza Cut Dahlia Iskandar Darmawi Darmawi Debby Novita ayumi Dian Masyitha Dian Masyitha Dwinna Aliza Ekawati, Wahyu Enitan, Seyi S. Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Ezy Yulanda Rezki Fadli A Gani Fadli A. Gani Fadli A. Gani Fadli A. Gani Fadli. A. Gani Fahrezi, Muhammad Gilbran Faisal Jamin Faisal Mustafa Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Ferllya Adela Putri Fitriani Fitriani Fitriani Fitriani Frengki Frengki Gholib Gholib Hafidh Hasan Hafizuddin Hafizuddin Hamdani Budiman Hasriati Hasriati Hendri Hermawan Adinugraha Herlina Dimiati, Herlina Herlina Yuliani Herrialfian Herrialfian I. Djuwita . I.Nasution . Idawati Nasution Idawati Nasution Idawati Nasution Idawati Nasution Isky, Saiful Ismail Ismail Ismail Ismail Ita Djuwita Ita Djuwita Joharsyah J Juli Melia Kamarlis, Reno K. Ketut Adnyane Mudite Kusdiantoro Mohamad Lailia Dwi Kusuma Wardhani Lailia Dwi Kusuma Wardhani Lidya Elizabeth M. Manik M. Hasan M. Isa M. Isa M. Jalaluddin Mahdi Abrar Mahdi Abrar Mahdi Abrar Makmur, Ali Manfarizah Manfarizah Maryulia Dewi Mudatsir Mudatsir Mudhita Zikkrullah Ritonga MUHAMMAD AGIL Muhammad Hambal Muhammad Hambal Muhammad Iqbal Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muhammad Zulfan Mulyadi Adam Mulyadi Adam Mulyadi Adam Muslim Akmal Muslim Akmal Mustafa Sabri Mustafa Sabri Mustafa Sabri Muttaqien Muttaqien Nabila Latifa Hafizsha Nadia Hanifah Nanda Yulian Syah Nauval Gibran Lubis Noni Zakiah Nurhidayat, Nurhidayat Nuzul Asmilia Patrick Flaggellata Prabowo Purwono Putro Primaadhi Abimanyu Satrio Raharjo Rastina Rastina Razali Daud Rinidar R Rinidar Rinidar Ritonga, Mudhita Zikrullah Roslizawaty R Rosmaidar Rosmaidar S. Agungpriyono . Safni Kamal Saputra, Jefri Dwi Savitri Novelina Shinta Mutia RM Siti Aisyah Sri Haryani Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Sugito Sugito Sulasmi Sulasmi Suriadi S Syafruddin S Syafruddin Syafruddin Syakur Syakur T. Armansyah T. Armansyah T. Armansyah, TR Tanjung, Fitri Aisah Taufan Hidayat Taufan Hidayat Taufan Hidayat Taufan Hidayat Taufan Hidayat Teti Arabia Teuku Heriansyah, Teuku Teuku Reza Ferasyi Tongku Nizwan Siregar Triva Murtina Lubis Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ummu Balqis W.E. Prasetyaningtyas . Wahono Esthi Prasetyaningtyas Wahyu Eka Sari Wahyuni, Sri Wardhani, Lailia Dwi Kusuma Wasmen Manalu Yulisma, Rika Yusnizar Yusnizar Z Zainabun Zainabun Zainabun Zainabun Zainabun Zainuddin Z Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zuhrawati Zuhrawati Zuraidawati Zuraidawati