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The Expression of Type III Collagen Connective Tissue inGluteobiceps and Psoas Mayor Muscles of Aceh Cattle Tanjung, Fitri Aisah; Ali, Ihfan Khaidir; Jalaluddin, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Hidayat, Taufan; Wardhani, Lailia Dwi Kusuma; Ritonga, Mudhita Zikrullah; Rahmi, Erdiansyah; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Sofyan, Hamny
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.112-118

Abstract

Collagen is one of the determinant factors of beef tenderness. This study analyzed the expression and content of type III collagen connective tissue in two muscle types of Aceh cattle through immunohistochemical staining. Meat samples were obtained from the gluteobiceps and psoas major muscles of three Aceh cattle bulls weighing 250-300 kg, with a body condition score of three. Histology preparations were created from samples and subjected to immunohistochemical staining utilizing specific antibodies targeting type III collagen. Qualitative data were analyzed descriptively, while quantitative data were analyzed using IMAGE J software. The results showed that the expression of type III collagen connective tissue was found in the endomysium and perimysium layers of both muscles. The expression of type III collagen connective tissue varied significantly among the muscles (P<0.05). The gluteobiceps muscle had an average percentage of the area containing type III collagen connective tissue of 4.47±2.41%, while the psoas major muscle was lower at 2.51±1.69%. This study concludes that the gluteobiceps muscle of Aceh cattle has a higher amount of type III collagen connective tissue than the psoas major muscle.
Ascorbic acid and calcitriol as alternative preventive strategies for myocardial damage in type 2 diabetes mellitus: an in vivo study using diabetic and atherosclerotic rat models Saputra, Jefri Dwi; Heriansyah, Teuku; Sofyan, Hamny; Dimiati, Herlina; Mudatsir, Mudatsir
Heart Science Journal Vol. 6 No. 1 (2025): Challenges in Managing Acute Heart Failure
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2025.006.01.14

Abstract

BACKGROUND: Type 2 diabetes mellitus can increase the production of reactive oxygen species (ROS) and genetic transcription factors, such as nuclear factor kappa beta (NF-κB). These phenomena may enhance the progression of atherosclerosis. Additionally, NF-κB can induce apoptosis of pancreatic beta cells and accelerate disease progression. OBJECTIVE: To investigate the role of calcitriol and ascorbic acid on NF-κβ expression in vivo in aortic and myocardial tissues of Wistar rats. METHODS: This randomized experimental study involved 24 male Wistar rats of the Rattus norvegicus strain, divided into four groups: NC (negative control), PC (positive control), AG (ascorbic acid group), and CG (calcitriol group). The study spanned 90 days, including a 30-day intervention period. Aortic and myocardial samples were processed into histological preparations and stained using immunohistochemical techniques. NF-κB expression was assessed using an intensity scoring method. RESULTS: The CG group demonstrated the lowest NF-κB immunoexpression in myocardial tissue (0.27 ± 0.08), followed by the AG (0.37 ± 0.05), NC (0.68 ± 0.08), and PC (1.13 ± 0.34) groups, with statistically significant 3sbetween the groups (p < 0.05). In aortic tissue, the CG group also exhibited the lowest NF-κB expression (0.30 ± 0.18), followed by the AG (0.50 ± 0.09), NC (0.97 ± 0.05), and PC (1.23 ± 0.38) groups, with statistically significant differences between the groups (p < 0.05).  Similar trends were observed in the immunohistochemical staining of the NF-κB antigen in myocardial and aortic tissue samples. Calcitriol administration was more effective than ascorbic acid in reducing NF-κB expression in both myocardial (p < 0.05) and aortic tissues (p < 0.05). CONCLUSION: Both ascorbic acid and calcitriol reduce NF-κB expression in the aorta and myocardium, with calcitriol showing greater effectiveness than ascorbic acid.
MICROSTRUCTURAL CHARACTERISTICS OF THE Longissimus dorsi MUSCLE IN BRAHMAN CROSS CATTLE Sofyan, Hamny; Jalaluddin, Muhammad; Rahmi, Erdiansyah; Wahyuni, Sri; Hidayat, Taufan; Adam, Mulyadi; Gani, Fadli A; Sabri, Mustafa; Ritonga, Mudhita Zikkrullah
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i2.25885

Abstract

This study aimed to analyze the muscle microstructure of longissimus dorsi Brahman cross (BX) cattle slaughtered at the slaughterhouse of North Aceh District. The meat samples for the study were taken from three male BX cattle aged 18-30 months with body weights ranging from 400 kg to 500 kg and the average body condition scores (BCS) of 3-4. The muscle used was the longissimus dorsi muscle. The mean (SE) diameter and cross-sectional area (CSA) of muscle fibers and the percentage of fast fibers in the longissimus dorsi BX muscle were 71.691.23 m, 4263.43305.69 m2 , and 48.178.81%, respectively. This study concluded that the longissimus dorsi muscle of the BX has relatively large muscle fibers, some of which in each fascicle are composed of fast muscle (fast fiber/type II).
CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak) Wahyuni, Sri; Sofyan, Hamny; Hafizuddin, Hafizuddin; Adnyane, I Ketut Mudite; Gholib, Gholib; Jalaluddin, Muhammad; Gani, Fadli A.; Adam, Mulyadi; Siregar, Tongku Nizwan; Agungpriyono, Srihadi
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.24418

Abstract

The presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouins solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation.
Perbaikan pengelolaan sampah rumah tangga dan sampah kebun di Gampong Meunasah Papeun Kabupaten Aceh Besar [Improving household and garden waste management in Meunasah Papeun Village, Aceh Besar District] Hasan, Hafidh; Haryani, Sri; Zainabun, Z; Wahyuni, Sri; Sofyan, Hamny
Buletin Pengabdian Vol 4, No 2 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v4i2.38598

Abstract

The waste problem in Aceh Besar District has been going on for quite some time now, which root cause can be tracked back to the lack of waste collection and treatment facilities compared to its residents waste growth. Therefore, the amount of untreated waste continues to increase. Meunasah Papeun Village as one of the villages in Aceh Besar District also faces the same problem, where the facilities and capacity of the Village Waste Management Unit (VWM) cannot keep up with the growth of residents' waste. Untreated waste ends up in illegal dumps or is burned by residents. This has reduced the comfort and quality of the village environment. In this Community Development Program (CDP), an approach was taken to reduce the waste that ended up in landfills through education and training on recycling and composting. The process sorted waste into three categories: organic, non-organic and residual waste. Some organic waste was composted and the rest became residue. Non-organic waste was sorted into recyclable and residual groups. This CDP was carried out in collaboration with Bank Sampah USK (BSU) to collect residents' recyclable waste. The residual was collected by VWM to be sent into the landfill. The residents who participated in these activities were able to cut their waste from a third to more than half. The extra money from recycling that was deposited into BSU was an additional advantage.
Penyuluhan budidaya sayuran untuk meningkatkan gizi keluarga dan imunitas tubuh di masa pandemi Covid-19 di Gampong Limpok Kecamatan Darussalam (Counseling of vegetable cultivation to improve family nutrition and immunity in the era of Covid-19 pandemic in Limpok Village, Darussalam Sub-District) Zainabun, Zainabun; Yusnizar, Yusnizar; Syakur, Syakur; Arabia, Teti; Manfarizah, Manfarizah; Wahyuni, Sri; Hidayat, Taufan; Sofyan, Hamny
Buletin Pengabdian Vol 2, No 3 (2022): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v2i3.28826

Abstract

According to field observations showed that the community in Limpok Village, Darussalam Sub-District has not fully utilized their yards for growing vegetables. The purpose of this community service activity is to provide counseling on vegetable cultivation to improve family nutrition and their immunity in the Covid-19 era in Gampong Limpok. The dissemination of counseling materials to participants helped carry out the activity utilizing the counseling approach. The Community Service Team and the participants used interactive discussion techniques to gauge the participants' knowledge before and after counseling. The use of the yard for the production of vegetables and varieties of vegetables with high nutrition was discussed in the counseling materials. The women of PKK Gampong Limpok were the counseling's target audience. The participants in the outreach activities excitedly listened and comprehended the importance of vegetables in preserving body immunity in the face of the Covid-19 pandemic, as well as the correct stages of various vegetable growing. Following the guidance given by Community Service Team, it can be concluded that the participants have understood how to use their yard as a place for cultivating vegetables that can be used as a source of nutrition for families and to increase body immunity in the Covid-19 era and in daily life. Additionally, the participants hopes that the activities can continue with the direct technical practice of planting vegetables that have the potential to be cultivated.
Pemberdayaan Masyarakat Gampong Kuta Ateuh melalui Inovasi Pakan Berformulasi Maggot dan Daun Kari untuk Meningkatkan Produktivitas Ternak dan Kesejahteraan Peternak Wardhani, Lailia Dwi Kusuma; Muhammad Resthu; Hamny Sofyan; Fachrizal Ambia; Daniel, Daniel; Lexa Lady Syourin; Kimmy Keisha Avrila Rozandi; Syifa Aurelia Devina; Khairun Nisa
Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Vol. 7 No. 1 (2025): Volume 7, No 1, Desember 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/atjpm.v7i1.7433

Abstract

Program pengabdian masyarakat ini dilaksanakan untuk mengatasi permasalahan rendahnya produktivitas ternak unggas pada skala rumah tangga di Gampong Kuta Ateuh yang disebabkan oleh keterbatasan akses terhadap pakan berkualitas dan tingginya biaya pakan komersial. Kegiatan ini bertujuan memperkenalkan inovasi pakan alternatif berbahan dasar maggot Black Soldier Fly dan daun kari sebagai solusi ekonomis, mudah dibuat, dan berbasis potensi lokal. Metode pelaksanaan meliputi identifikasi kondisi lapangan, sosialisasi, pelatihan, demonstrasi, dan pendampingan pembuatan pakan inovasi MaKaFeed oleh dosen, mahasiswa, dan masyarakat serta pemanfaatannya ke ternak. Evaluasi dilakukan melalui pre–post test pelatihan, observasi performa ternak, serta wawancara dengan peternak. Kegiatan sosialisasi dilakukan pada 5 Oktober dengan 25 peserta dan dilanjutkan dengan kegiatan wawancara dengan peternak pada 5 November 2025. Kegiatan wawancara dilakukan untuk mengetahui hasil dan respon ternak dari pemberian MaKaFeed. Hasil kegiatan sosialisasi menunjukkan keberhasilan peserta dalam mempratikan pembuatan MaKaFeed dan adanya peningkatan signifikan pengetahuan peserta dari 8–42% sebelum pelatihan menjadi 90–100% setelah mengikuti pelatihan. Hasil wawancara menunjukan bahwa ternak merespon baik dari pakan MaKaFeed dengan adanya peningkatan nafsu makan dan berat badan serta jarang terkena penyakit dan peternak menyampaikan bahwa adanya pemberian MaKaFeed secara langsung dapat menurunkan biaya pakan. Kesimpulan dari kegiatan ini adalah inovasi pakan MAKaFeed yang berformula utama maggot BSF dan daun kari secara efektif meningkatkan produktifitas ternak dan juga kesejahteraan peternak Gampong Kuta Ateuh.
Gambaran Makroanatomi dan Mikroanatomi Bursa Fabrisius Ayam Pedaging yang Diberi Pakan Tepung Larva (Maggot) Black Soldier Fly Wardhani, Lailia Dwi Kusuma; Jalaluddin, M.; Firdausi, Nida Khofiya; Sofyan, Hamny; Masyitha, Dian; Wahyuni, Sri; Sabri, Mustafa; Rahmawati, Indra; Nussa, Olan Rahayu Puji Astuti; Ritonga, Mudhita Zikkrullah
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung larva (maggot) Black Soldier Fly (BSF) terhadap makroanatomi dan mikroanatomi serta interaksi organ bursa Fabrisius ayam pedaging umur berbeda. Penelitian ini menggunakan rancangan acak lengkap dengan dua faktor yaitu perlakuan maggot BSF dan umur ayam. Sampel penelitian menggunakan 24 ekor ayam pedaging. Faktor perlakuan maggot BSF sebanyak empat kelompok yang terdiri atas P0 (Pakan komersil 100%), P1 (Pakan komersil 90% + tepung maggot BSF 10%), P2 (Pakan komersil 80% + tepung maggot BSF 20%), dan P3 (Pakan komersil 70% + tepung maggot BSF 30%). Faktor umur ayam terbagi dua yaitu umur 14 hari dan 21 hari. Perlakuan penelitian selama 14 hari. Sampel ayam dinekropsi untuk diambil organ bursa Fabrisius. Sampel organ dibuat preparat histologi dan diwarnai dengan Hematoksilin-Eosin. Data warna dan bentuk bursa Fabrisius dianalisis secara deskriptif, sedangka data hasil pengukuran histologinya dianalisis menggunakan uji sidik ragam dua arah) dan uji Tukey. Hasil penelitian ini menunjukan bahwa pemberian pakan tepung maggot BSF dan umur ayam memberikan pengaruh pada gambaran makroanatomi bursa Fabrisius pada hasil tertinggi yaitu panjang (2,50 ± 0,07 cm), lebar (2,20 ± 0,10 cm), bobot (1,89 ± 0,511 g). Pada pengukuran mikroanatomi, bursa Fabrisius pemberian pakan tepung maggot BSF menunjukkan pengaruh pada panjang folikel (0,85 ± 0,02 mm), lebar folikel (0,44 ± 0,02 mm), serta tinggi plicae (4,84 ± 0,35 mm) dipengaruhi oleh perbedaan umur.. Interaksi antara faktor perlakuan dan umur ayam menunjukkan interaksi terhadap panjang, lebar dan bobot bursa, panjang dan lebar folikel bursa Fabrisius. Simpulan dari studi adalah pemberian tepung maggot BSF sebagai pakan ayam pedaging memberikan pengaruh  pada organ bursa Fabrisius dengan perlakuan terbaik pada perlakuan P3 atau ransum dengan 30% tepung maggot BSF dan 70% pakan komersial.
Co-Authors . Nurliana . Zuchri ., Daniel Abdul Harris Abdullah Hamzah Adi Purnawarman, Adi Ahmad Azhari Nopiosi Alfajri Saputra Alfajri Saputra Ali, Ihfan Khaidir Aliman Irfandi Amalia Sutriana Amiruddin A Andi Novita Anugrah Septian Aris Junaidi Ayu Agita Ginting Azhari Azhari Bambang Pontjo Priosoeryanto Boni Anggara Budianto Panjaitan Budianto Panjaitan Chairun Nisa Chika Nahara Ramadhanty Chintya Desfariza Cut Dahlia Iskandar Daniel Daniel Darmawi Darmawi Debby Novita ayumi Dian Masyitha Dian Masyitha Dian Masyitha Dwinna Aliza Ekawati, Wahyu Enitan, Seyi S. Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Ezy Yulanda Rezki Fachrizal Ambia Fadli A Gani Fadli A. Gani Fadli A. Gani Fadli A. Gani Fadli. A. Gani Fahrezi, Muhammad Gilbran Faisal Jamin Faisal Mustafa Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Ferllya Adela Putri Firdausi, Nida Khofiya Fitriani Fitriani Fitriani Fitriani Frengki Frengki Gani, Fadli A Gani, Fadli A. Gholib Gholib Gholib Gholib, Gholib Hafizuddin Hafizuddin Hamdani Budiman Hasan, Hafidh Hasriati Hasriati Hendri Hermawan Adinugraha Herlina Dimiati, Herlina Herlina Yuliani Herrialfian Herrialfian I. Djuwita . I.Nasution . Idawati Nasution Idawati Nasution Idawati Nasution Idawati Nasution INDRA RAHMAWATI Isky, Saiful Ismail Ismail Ismail Ismail Ita Djuwita Ita Djuwita Joharsyah J Juli Melia Kamarlis, Reno K. Ketut Adnyane Mudite Khairun Nisa Kimmy Keisha Avrila Rozandi Kusdiantoro Mohamad Lailia Dwi Kusuma Wardhani Lailia Dwi Kusuma Wardhani Lexa Lady Syourin Lidya Elizabeth M. Manik M. Hasan M. Isa M. Isa M. Jalaluddin M. Jalaluddin, M. Mahdi Abrar Mahdi Abrar Mahdi Abrar Makmur, Ali Manfarizah Manfarizah Manfarizah Manfarizah Maryulia Dewi Mudatsir Mudatsir Mudhita Zikkrullah Ritonga MUHAMMAD AGIL Muhammad Hambal Muhammad Hambal Muhammad Iqbal Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muhammad Resthu Muhammad Zulfan Mulyadi Adam Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Muslim Akmal Mustafa Sabri Mustafa Sabri Mustafa Sabri Muttaqien Muttaqien Nabila Latifa Hafizsha Nadia Hanifah Nanda Yulian Syah Nauval Gibran Lubis Noni Zakiah Nurhidayat, Nurhidayat Nussa, Olan Rahayu Puji Astuti Nuzul Asmilia Patrick Flaggellata Prabowo Purwono Putro Primaadhi Abimanyu Satrio Raharjo Rastina Rastina Razali Daud Rinidar R Rinidar Rinidar Ritonga, Mudhita Zikkrullah Ritonga, Mudhita Zikrullah Roslizawaty R Rosmaidar Rosmaidar S. Agungpriyono . Safni Kamal Saputra, Jefri Dwi Savitri Novelina Shinta Mutia RM Siti Aisyah Sri Haryani Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Sugito Sugito Sulasmi Sulasmi Suriadi S Syafruddin S Syafruddin Syafruddin Syakur Syakur Syakur Syakur Syifa Aurelia Devina T. Armansyah T. Armansyah T. Armansyah, TR Tanjung, Fitri Aisah Taufan Hidayat Taufan Hidayat Taufan Hidayat Taufan Hidayat Taufan Hidayat Teti Arabia Teti Arabia Teuku Heriansyah, Teuku Teuku Reza Ferasyi Tongku Nizwan Siregar Triva Murtina Lubis Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ummu Balqis W.E. Prasetyaningtyas . Wahono Esthi Prasetyaningtyas Wahyu Eka Sari Wahyuni, Sri Wardhani, Lailia Dwi Kusuma Wasmen Manalu Yulisma, Rika Yusnizar Yusnizar Yusnizar Yusnizar Zainabun Zainabun Zainabun Zainabun Zainabun Zainabun Zainabun, Z Zainuddin Z Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zuhrawati Zuhrawati Zuraidawati Zuraidawati