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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Agro Teknologi Tropika Jurnal Pilar Sains Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan JURNAL AGROTEKNOLOGI Jurnal Pendidikan Biologi Indonesia Jurnal Agro BIO-SITE |BIOLOGI Sains Terapan Journal of Natural Science and Integration Pendas : Jurnah Ilmiah Pendidikan Dasar JMM (Jurnal Masyarakat Mandiri) Bio-Lectura Perspektif Pendidikan dan Keguruan SCAFFOLDING: Jurnal Pendidikan Islam dan Multikulturalisme Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Journal of Innovation in Educational and Cultural Research BIOGENESIS NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Sagu Journal Of Human And Education (JAHE) Jurnal Teknologi Pangan Journal of Educational Sciences Biology and Education Journal (BaEJ) JURNAL BIOLOGI PAPUA Jurnal Penelitian Kehutanan Wallacea Dinamika Pertanian Biodik: Jurnal Ilmiah Pendidikan Biologi
Imam Mahadi
Faculty Of Education And Teacher Training, University Riau, Pekanbaru, 28293, Indonesia.
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Journal : JURNAL BIOLOGI PAPUA

 1 
Ekstraksi DNA Nibung (Oncosperma tigillarium (Jack) Ridl.) Menggunakan Beberapa Jenis Buffer Terhadap Kualitas Genomik DNA Mahadi, Imam; Sayuti, Irda; Nursal, Nursal
Jurnal Biologi Papua Vol 16 No 2 (2024)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.3780

Abstract

The Nibung (Oncosperma tigillarium (Jack) Ridl.) is a plant that serves as the floral mascot of Riau and symbolizes the spirit of unity and brotherhood among its people. Genetic diversity research is essential for the conservation of this species, with DNA extraction being a critical initial step. This study compared three types of buffers: CTAB, Alkyl Benzene Sulfonate (ABS) surfactant detergent, and Sodium Laureth Sulfate (SLS) surfactant detergent. The aim of this research was to identify alternative buffers to replace CTAB and simplify the extraction of genomic DNA from Nibung. Parameters measured included the presence of DNA and DNA concentration, using electrophoresis and a nanodrop spectrophotometer. The results showed that clear genomic DNA bands were successfully obtained for 24 Nibung accessions, with only two accessions, Nibung2s and Nibung6s, showing smeared bands when using the SLS surfactant detergent buffer. The genomic DNA concentrations ranged from 526.2 to 3,829.2 ng/µl. These concentrations are suitable for use as DNA templates in further research. Thus, both ABS and SLS surfactant detergent buffers can be considered viable alternatives to CTAB for extracting genomic DNA from Nibung.  Key words: Buffer; DNA bands; DNA extraction; DNA Concentration; Extraction optimization.
Co-Authors ', Darmadi ', Darmawati ', Hermiyati ', Nurkameria Addarwida Omar ' Alaniyah Syafaren Alaniyah Syafaren Amelia Prananda Apriyani " Apriyani ' Arasyid, Hari Ariska, Devi Arnentis Arnentis Arnentis Arnentis Asih Rahayu Ajeng Agesti Astati, Rita Azizah Safitri Bakhendri Solfan Berlian Kumala ' Br Purba, Salmiah Chrisdayanti Br Simanjuntak Darmadi Darmadi Darmawati " Darmawati ' Darmawati Darmawati Darmawati Darmawati Darmawati Darmawati Dedy Purnomo Delfi Trisnawati Desrianty Syafitri Desta Manulang DEVIANA DEVIANA Diana Anggraini Edri Yandi Edri Yandi Yandi, Edri Yandi Elya Febrita Enita Sari Evi Suryawati Evi Suryawati Evi Suryawati Evi Suryawati Suryawati, Evi Suryawati Fadli, Fery Fajriyah Wirawati Febblina Daryanes Febrina, Hazirah Feli Setiadewi Setiadewi, Feli Setiadewi Fenny Nadya Anggraeni Firman syah Fitra Suzanti Fitria Lisni Purba Fitriya, Anisa Fitriya, Gita Fraditia Wiranti Futtuhur Rahmah Geni Meiry Sukma Meiry Sukma, Geni Meiry Sukma Meiry Geny Meiry Sukma Guntur, Geby Zaryani Habibah, Irma Haidi Haidi Haidi, Haidi Halawa, Astika Sari Harlis Harlis Harlis Harlis Hesti Kharisma Kharisma, Hesti Kharisma Iis Fitriani Indri Rahmawati Irda Sayuti Irda, Sayuti Irma Habibah Jamhari Jamhari Juana Prawita Julia Riska Junaidi, Annajmu Sasna Khoirunnisya Dalimunthe Kusmedi, Hana Tasya Kusnedi, Hana Tasya Lesmanawati, Sri Eggi Lilia Halim Lilik Noviawati Lilis Setiawati Lisa Indriani M. Irsyad Nur Maharani, Helva Mariani Natalina L Mariani Natalina Linggasari Mariani Natalina Linggasari Marni Alhuda Alhuda Megawati Anggraini Mellisa, Novyona Ayu Monalysa Monalysa Muhammad Darus Muhammad Rivai Muliana gultom Nada Haini Nisa Rahmawati, Nisa Nita Triana Novyona Ayu Mellisa Nur Halimah Nur Halimah Nurahma Dini Nurhadiyah ' Nurhayati Nurhayati Nurkameria ' Nurkameria Nurkameria Nurlinda ' Nursal ' Nursal Nursal Nursal Nursal Nursal Nursal, Nursal Nurul Hasanah Oky Priawan Pranto Wati Putri Ramadhani Putri, Widya Rabia Hidayanti Arnan Rabia Hidayanti Arnan, Rabia Hidayanti Rahmi, Fitri Olvia Rendi Handika Ria Syafitri Rika Mulyani Rika Mulyani Rio Rasdian Saputra Riska, Julia Romita Romita Safitri, Widia Satifa, Nabela Ida Coriza Sheliana, Yolanda Siagian, Lili Rahma Silvy Rachmadani Octavia Sirait, Rapika Siregar, Anna Sherley SRI RAHAYU Sri Wilda Yanti Sri Wulandari SRI WULANDARI Sri Wulandari Sri Wulandari Sri Wulandari Sri Wulandari Sri Wulandari ' Sri Wulandari Wulandari Sri Wulandari Wulandari, Sri Wulandari Suci Agustiani Suci Agustiani Agustiani Surani Ariyana Suwondo Suwondo Suzanti , Fitra Syafaren, Alaniyah Syafi’i, Wan Syafitri, Desrianty Syafi’i, Wan Syafi’i, Wan Syahrul Ramadhan Ramadhan, Syahrul Ramadhan Timbir Manik Tini Anggraini Simamora Tri Beti Indri Utami, Dian Putri Vebrianto, Rian Wahyuni, Resma Wan Rosliana Wan Safii Wan Syafi'i Wan Syafi’i Wan Syafii Wan Syafii Wan Syafi’i Wan Syafi’i Wan Syafi’i Wan Syafi’i syafi'i Wansyafii Wansyafii Waruwu, Dasari Wenny Anggraini Wira, Yasli Wirawati, Fajriyah Wulan Indri Safitri Indri Safitri, Wulan Indri Safitri Indri Wulan Sari Yeni Sari Yeni Sari, Yeni Yeyen Kaswita Kaswita Yoana Ulitua Panggabean Yohana Pertiwi Yolanda, Mercy Yunita Kesia S Yuslim Fauzia Fauzia Yuslim Fauziah Yustina Yustina Yustina Yustina Yustina Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina, Zulfarina