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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Agro Teknologi Tropika Jurnal Pilar Sains Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan JURNAL AGROTEKNOLOGI Jurnal Pendidikan Biologi Indonesia Jurnal Agro BIO-SITE |BIOLOGI Sains Terapan Journal of Natural Science and Integration Pendas : Jurnah Ilmiah Pendidikan Dasar JMM (Jurnal Masyarakat Mandiri) Bio-Lectura Perspektif Pendidikan dan Keguruan SCAFFOLDING: Jurnal Pendidikan Islam dan Multikulturalisme Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Journal of Innovation in Educational and Cultural Research BIOGENESIS NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Sagu Journal Of Human And Education (JAHE) Jurnal Teknologi Pangan Journal of Educational Sciences Biology and Education Journal (BaEJ) JURNAL BIOLOGI PAPUA Jurnal Penelitian Kehutanan Wallacea Dinamika Pertanian Biodik: Jurnal Ilmiah Pendidikan Biologi
Imam Mahadi
Faculty Of Education And Teacher Training, University Riau, Pekanbaru, 28293, Indonesia.
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Journal : Jurnal Agro

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Kultur suspensi sel tanaman gajah beranak (Goniothalamus tapis Miq) terhadap kandungan zat goniotalamin Imam Mahadi; Sri Wulandari; Wan Safii; Irda Sayuti
Jurnal Agro Vol 8, No 2 (2021)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/14710

Abstract

Zat goniotalamin pada tanaman gajah beranak (Goniothalamus tapis) merupakan obat alternatif penyembuhan kanker. Penelitian bertujuan untuk mendapatkan zat goniotalamin melalui kultur kalus dan kultur suspensi sel. Metode penelitian eksperimen Rancangan Acak Lengkap (RAL) dengan kombinasi 2,4-D (1-10 mgL-1) dan BAP (0,5-2 mgL-1) menggunakan eksplan batang muda, terdiri dari 17 perlakuan dan 3 kali ulangan. Analisis data menggunakan Analysis of Variances dan uji lanjut Duncan Multiple Range Test (DMRT) taraf 5%. Hasil menunjukkan bahwa kultur kalus G. tapis pada media 5,0 mg L-1 2,4-D + 1 mg L-1 BAP adalah yang terbaik dengan waktu muncul kalus 28,33 hari dan persentase pembentukan kalus 100%. Kalus untuk kultur suspensi sel bertekstur remah dan berwarna kuning kehijauan. Kultur suspensi sel menghasilkan pertumbuhan sel yang cepat, tidak lembek berair dan mudah dipisahkan. Hasil kualitatif Kromatografi Lapis Tipis kultur suspensi sel sangat jelas, bersih dan terdapat potensi kandungan zat goniotalamin pada perlakuan 2,4-D 5 mg L-1 + BAP 0,5 mg L-1, 2,4-D 5 mg L-1 + BAP 1 mg L-1, 2,4-D 5 mg L-1 + BAP 2 mg L-1, 2,4-D 10 mg L-1 + BAP 0,5 mg L-1 dan 2,4-D 10 mg L-1 + BAP 1 mg L-1. Hasil kuantitatif zat goniotalamin dengan Kromatografi Cair Prestasi Tinggi terdapat pada perlakuan 2,4-D 5,0 mgL-1 + BAP 1 mg L-1 yaitu 9,57 mg g-1.The goniothalamine compound on Goniothalamus tapis is an alternative cancer medicine. This study aimed to obtain gonotalamin through callus culture and suspension cell culture. The experiment research method was Completely Randomized Design (CRD) with a combination of 2.4-D (1-10 mg L-1) and BAP (0.5-2 mg L-1) using young stem explants consisting of 17 treatments with 3 replications. Data analysis used ANOVA and DMRT at 5%. The results showed that G. tapis callus culture on 5.0 mg L-1 2.4-D + 1 mg L-1 BAP was the best treatment medium with callus emergence time of 28.33 days and percentage of callus formation 100%. The callus used for suspension cell culture was friable and greenish-yellow in color. Suspension cell culture resulted in rapid cell growth, was not fleshy, and easily separated. The  quality test by Thin Layer Chromatography (TLC) from suspension cell culture resulted very clear, clean, and potential content of goniothalamin found in treatments 2.4-D 5.0 mg L-1 + BAP 0.5 mg L-1, 2.4-D 5.0 mg L-1 + BAP 1 mg L-1, 2.4-D 5.0 mg L-1 + BAP 2 mg L-1, 2.4-D 10 mg L-1 + BAP 0.5 mg-1 and 2.4-D 10 mg-1 + BAP 1 mg-1. The quantitative results of the best goniotalamine compounds in cell suspension cultures using High-Performance Liquid Chromatography (HPLC) on medium 2,4-D 5.0 mgL-1 + BAP 1 mg L-1 ie 9.57 g-1.
Co-Authors ', Darmadi ', Darmawati ', Hermiyati ', Nurkameria Addarwida Omar ' Alaniyah Syafaren Alaniyah Syafaren Amelia Prananda Apriyani " Apriyani ' Arasyid, Hari Ariska, Devi Arnentis Arnentis Arnentis Arnentis Asih Rahayu Ajeng Agesti Astati, Rita Bakhendri Solfan Berlian Kumala ' Br Purba, Salmiah Chrisdayanti Br Simanjuntak Darmadi Darmadi Darmawati " Darmawati ' Darmawati Darmawati Darmawati Darmawati Darmawati Darmawati Dedy Purnomo Delfi Trisnawati Desrianty Syafitri Desta Manulang DEVIANA DEVIANA Diana Anggraini Edri Yandi Edri Yandi Yandi, Edri Yandi Elya Febrita Enita Sari Evi Suryawati Evi Suryawati Evi Suryawati Evi Suryawati Suryawati, Evi Suryawati Fadli, Fery Fajriyah Wirawati Febblina Daryanes Febrina, Hazirah Feli Setiadewi Setiadewi, Feli Setiadewi Fenny Nadya Anggraeni Firman syah Fitra Suzanti Fitria Lisni Purba Fitriya, Anisa Fitriya, Gita Fraditia Wiranti Futtuhur Rahmah Geni Meiry Sukma Meiry Sukma, Geni Meiry Sukma Meiry Geny Meiry Sukma Guntur, Geby Zaryani Habibah, Irma Haidi Haidi Haidi, Haidi Halawa, Astika Sari Harlis Harlis Harlis Harlis Hesti Kharisma Kharisma, Hesti Kharisma Iis Fitriani Indri Rahmawati Irda Sayuti Irda, Sayuti Irma Habibah Jamhari Jamhari Juana Prawita Julia Riska Junaidi, Annajmu Sasna Khoirunnisya Dalimunthe Kusmedi, Hana Tasya Kusnedi, Hana Tasya Lesmanawati, Sri Eggi Lilia Halim Lilik Noviawati Lilis Setiawati Lisa Indriani M. Irsyad Nur Maharani, Helva Mariani Natalina L Mariani Natalina Linggasari Mariani Natalina Linggasari Marni Alhuda Alhuda Megawati Anggraini Mellisa, Novyona Ayu Monalysa Monalysa Muhammad Darus Muhammad Rivai Muliana gultom Nada Haini Nita Triana Novyona Ayu Mellisa Nur Halimah Nur Halimah Nurahma Dini Nurhadiyah ' Nurhayati Nurhayati Nurkameria ' Nurkameria Nurkameria Nurlinda ' Nursal ' Nursal Nursal Nursal Nursal Nursal Nursal, Nursal Nurul Hasanah Oky Priawan Pranto Wati Putri Ramadhani Putri, Widya Rabia Hidayanti Arnan Rabia Hidayanti Arnan, Rabia Hidayanti Rahmi, Fitri Olvia Rendi Handika Ria Syafitri Rika Mulyani Rika Mulyani Rio Rasdian Saputra Riska, Julia Romita Romita Safitri, Widia Satifa, Nabela Ida Coriza Sheliana, Yolanda Siagian, Lili Rahma Silvy Rachmadani Octavia Sirait, Rapika Siregar, Anna Sherley SRI RAHAYU Sri Wilda Yanti Sri Wulandari Sri Wulandari Sri Wulandari Sri Wulandari Sri Wulandari SRI WULANDARI Sri Wulandari ' Sri Wulandari Wulandari Sri Wulandari Wulandari, Sri Wulandari Suci Agustiani Suci Agustiani Agustiani Surani Ariyana Suwondo Suwondo Suzanti , Fitra Syafaren, Alaniyah Syafi’i, Wan Syafitri, Desrianty Syafi’i, Wan Syafi’i, Wan Syahrul Ramadhan Ramadhan, Syahrul Ramadhan Timbir Manik Tini Anggraini Simamora Tri Beti Indri Utami, Dian Putri Vebrianto, Rian Wahyuni, Resma Wan Rosliana Wan Safii Wan Syafi'i Wan Syafi’i Wan Syafii Wan Syafii Wan Syafi’i Wan Syafi’i Wan Syafi’i Wan Syafi’i syafi'i Wansyafii Wansyafii Waruwu, Dasari Wenny Anggraini Wira, Yasli Wirawati, Fajriyah Wulan Indri Safitri Indri Safitri, Wulan Indri Safitri Indri Wulan Sari Yeni Sari Yeni Sari, Yeni Yeyen Kaswita Kaswita Yoana Ulitua Panggabean Yohana Pertiwi Yolanda, Mercy Yunita Kesia S Yuslim Fauzia Fauzia Yuslim Fauziah Yustina Yustina Yustina Yustina Yustina Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina, Zulfarina