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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Indonesian Journal of Electrical Engineering and Computer Science Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
M. Zairin Junior
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Social Sciences Veterinary Other

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Journal : Indonesian Aquaculture Journal

 1 
MORPHOLOGICAL CHARACTERISTIC OF SPERMATOGONIA AND TESTES DISSOCIATION : A Preliminary Study for the Germ Cell Transplantation in Giant Gouramy (Osphronemus gouramy) Irma Andriani; Ita Djuwita; Komar Sumantadinata; Muhammad Zairin Junior; Harton Arfah; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 5, No 2 (2010): (December 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.767 KB) | DOI: 10.15578/iaj.5.2.2010.163-172

Abstract

The recent study were attempting to develop spermatogonial germ cell transplantation as a tool to preserve and propagate male germ-plasm from endangered fish species, as well as to produce surrogate broodstock of commercially valuable fish. Spermatogonia identification and testes dissociation were the first necessary steps to obtain highly amount and viable population of spermatogonia as donor cells for transplantation. Using giant gouramy testes as a model, spermatogonia was histological characterized and two methods of testes dissociations were compared (i.e. medium A contained 0.5% trypsin in PBS and medium B contained 0.5% trypsin and DNase 10 IU/μL in PBS complemented with CaCl2, Hepes and FCS). Optimal incubation times (1, 2, 3, 4 and 5 hours) in dissociation medium were also determined. Freshly isolated testes of immature giant gouramy were minced in dissociation medium and then incubated to get monodisperce cell suspension. Parameters observed were number and viability of spermatogonia (ø > 10 μm). The viability was analyzed using trypan blue exclusion dye. The results showed that the average number of spermatogonia observed in medium B was higher than in medium A (P<0.05), meanwhile the viability of spermatogonia between medium A and B were not significantly different (P>0.05). The viability of spermatogonia decreased by the increasing duration time of dissociation. The viability of spermatogonia started to decrease significantly in 2 hours incubation time in medium A and 4 hours incubation time in medium B (P<0.05). In conclusion, application of dissociation medium B yielded higher number of viable spermatogonia than dissociation medium A.
CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Heltonika, Benny; Sudrajat, Agus Oman; Junior, Muhammad Zairin; Widanarni, Widanarni; Suprayudi, Muhammad Agus; Manalu, Wasmen; Hadiroseyani, Yani
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.27-35

Abstract

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles.  The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments.  However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish. 
Co-Authors , Alimuddin . Hermawan . Sukenda . Suriansyah . Syafiuddin . Tarsim ., Mulyasari A M Tahya A S Mubarak A. Oman Sudrajat A. Shofy Mubarak A. Yunianti Aarab, Zineb Ade Sunarma Ade Sunarma Ade Sunarma, Ade Adharto Utiah Agus O . Sudradjat Agus Oman Sudrajat Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin . Alimuddin Alimuddin Alimuddin Alimuddin alimuddin alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anouar Darif Aprelia Martina Tomasoa, Aprelia Martina Arroub, Omar Asep Sopian Avarre, Jean-Christophe Azis Azis Bambang Purwantara Benny Heltonika Bethsy J. Pattiasina Cahyono, Tatak Dwi D. Nurlestiyoningrum D.S. Sjafei Damiana Rita Ekastuti DEDI JUSADI Dian Hardiantho Dian Hardianto Didik Ariyanto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dita Puji Laksana Dwi Hany Yanti E. Riani Eddy Supriyono Enang Harris Surawidjaja Etty Riani Fajar Basuki Fajar Maulana . Farizah, N Fauzan, Agung Luthfi Flandrianto S. Palimirmo GORO YOSHIZAKI H. Arfah Hamsah Hamsah Harton Arfah I Khasani I MADE ARTIKA I Wayan Nurjaya I. Herviani I. Mokoginta I. Supriatna Iis Diatin Ikhsan Khasani Imron Imron, Imron Irma Andriani IRMA ANDRIYANI Irvan Faizal Irzal Effendi Iskandar, Andri Ita Djuwita Jamaluddin Jompa Jasmanindar, Yudiana Jean-Christophe Avarre Julie Ekasari K Nisaa K. Sumantadinata Kadarusman . Khairun Nissa Khasani, I Komar Sumantadinata Kukuh Adiyana Kukuh Nirmala Kusman Sumawidjaja Laurent Pouyaud Livana Dethris Rawung Lolita Thesiana M Yamin M Yamin M. Bintang M. M. Raswin M. Raswin M. Sakdiah M. Syukur M. Toelihere M. Tri Djoko Sunarno M.M. Raswin Meilisza, Nina Mia Setiawati Mozes R . Toelihere MOZES R. TOELIHERE Mubarak, A S MUHAMMAD AGUS SUPRAYUDI Muhammad Muhammad Muhammad Safir Mulyasari . Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim N Farizah N. Potalangi N.B.P Utomo Nababan, Yanti Inneke Nasrullah, Hasan Naufal, Muhammad Restya Neviaty P. Zamani Nisaa, K Nugraha, Media Fitri Isma Nunak Nafiqoh Nur Bambang Priyo Utomo Nurbariah Nurbariah O. Carman Odang Carman Odilia Rovara Poppy Dea Bertha, Poppy Dea Prassetyo Dwi Dhany Wijaya Purnamawati Purnamawati Purnamawati Purnamawati Putri Zulfania, Putri R. Affandi R.G. Pahlawan R.K. Sari R.R Sri Pudji R.R.S.P.S. Dewi Rahmani, My Driss Rahminiwati, Min Rakhmawati, Rakhmawati, RIDWAN AFFANDI Robin . Robin . Roza Elvyra S. Darwisito S. Dewi S. Handayani S. Mulyati S. Purwati Safira Qisthina Ayuningtyas, Safira Qisthina Siti Subaidah Sri Nuryati Sri Nuryati Srihadi Agungpriyono Suci Antoro Sugeng Budiharsono Sujaka Nugraha Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Syafyudin Yusuf T.L. Yusuf Tahya, A M Tarsim Tarsim TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Triayu Rahmadiah Tuty L . Yusuf W. Manalu Wahyu Pamungkas Wasmen Manalu WIDANARNI WIDANARNI Wisriati Lasima Wiyoto Wiyoto Wiyoto, W. Y. Hadiroseyani Y. Yustikasari Yogi Himawan Yudha Trinoegraha Adiputra Yusnarti Yus Zahri, Abdul