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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Civil Engineering Journal Proceeding Simposium Nasional Kelautan dan Perikanan Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Indonesian Journal of Electrical Engineering and Computer Science Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
M. Zairin Junior
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Social Sciences Veterinary Other

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Journal : HAYATI Journal of Biosciences

 1 
Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine- . ALIMUDDIN; IRMA ANDRIYANI; MUHAMMAD ZAIRIN JUNIOR; HARTON ARFAH; ANNA OCTAVERA; ODANG CARMAN; GORO YOSHIZAKI
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.135

Abstract

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.
Co-Authors , Alimuddin . Hermawan . Sukenda . Suriansyah . Syafiuddin . Tarsim ., Mulyasari A M Tahya A S Mubarak A. Oman Sudrajat A. Shofy Mubarak A. Yunianti Aarab, Zineb Ade Sunarma Ade Sunarma Ade Sunarma, Ade Adharto Utiah Agus O . Sudradjat Agus Oman Sudrajat Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin . Alimuddin Alimuddin Alimuddin Alimuddin alimuddin alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anouar Darif Aprelia Martina Tomasoa, Aprelia Martina Arroub, Omar Asep Sopian Avarre, Jean-Christophe Azis Azis Bambang Purwantara Benny Heltonika Bethsy J. Pattiasina Cahyono, Tatak Dwi D. Nurlestiyoningrum D.S. Sjafei Damiana Rita Ekastuti DEDI JUSADI Dian Hardiantho Dian Hardianto Didik Ariyanto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dita Puji Laksana Dwi Hany Yanti E. Riani Eddy Supriyono ElAroussi, Mohamed Enang Harris Surawidjaja Etty Riani Fadel, Mohamed T. Fajar Basuki Fajar Maulana . Farizah, N Fauzan, Agung Luthfi Flandrianto S. Palimirmo GORO YOSHIZAKI H. Arfah Hamsah Hamsah Harton Arfah I Khasani I MADE ARTIKA I Wayan Nurjaya I. Herviani I. Mokoginta I. Supriatna Iis Diatin Ikhsan Khasani Imron Imron, Imron Irma Andriani IRMA ANDRIYANI Irvan Faizal Irzal Effendi Iskandar, Andri Ita Djuwita Jamaluddin Jompa Jasmanindar, Yudiana Jean-Christophe Avarre Julie Ekasari K Nisaa K. Sumantadinata Kadarusman . Khairun Nissa Khasani, I Komar Sumantadinata Kukuh Adiyana Kukuh Nirmala Kusman Sumawidjaja Laurent Pouyaud Livana Dethris Rawung Lolita Thesiana M Yamin M Yamin M. Bintang M. M. Raswin M. Raswin M. Sakdiah M. Syukur M. Toelihere M. Tri Djoko Sunarno M.M. Raswin Meilisza, Nina Mia Setiawati Mozes R . Toelihere MOZES R. TOELIHERE Mubarak, A S MUHAMMAD AGUS SUPRAYUDI Muhammad Muhammad Muhammad Safir Mulyasari . Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim N Farizah N. Potalangi N.B.P Utomo Nababan, Yanti Inneke Nasrullah, Hasan Naufal, Muhammad Restya Neviaty P. Zamani Nisaa, K Nugraha, Media Fitri Isma Nunak Nafiqoh Nur Bambang Priyo Utomo Nurbariah Nurbariah O. Carman Odang Carman Odilia Rovara Poppy Dea Bertha, Poppy Dea Prassetyo Dwi Dhany Wijaya Purnamawati Purnamawati Purnamawati Purnamawati Putri Zulfania, Putri R. Affandi R.G. Pahlawan R.K. Sari R.R Sri Pudji R.R.S.P.S. Dewi Rahmani, My Driss Rahminiwati, Min Rakhmawati, Rakhmawati, Rguig, Mustapha RIDWAN AFFANDI Robin . Robin . Roza Elvyra S. Darwisito S. Dewi S. Handayani S. Mulyati S. Purwati Safira Qisthina Ayuningtyas, Safira Qisthina Siti Subaidah Sri Nuryati Sri Nuryati Srihadi Agungpriyono Suci Antoro Sugeng Budiharsono Sujaka Nugraha Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Syafyudin Yusuf T.L. Yusuf Tahya, A M Tarsim Tarsim TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Triayu Rahmadiah Tuty L . Yusuf W. Manalu Wahyu Pamungkas Wasmen Manalu WIDANARNI WIDANARNI Wisriati Lasima Wiyoto Wiyoto Wiyoto, W. Y. Hadiroseyani Y. Yustikasari Yogi Himawan Yudha Trinoegraha Adiputra Yusnarti Yus Zahri, Abdul