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UJI EFEKTIVITAS EKSTRAK METANOL 80% BLACK GARLIC TUNGGAL SEBAGAI ANTIHIPERURISEMIA TERHADAP TIKUS: EFFECTIVENESS TEST OF 80% METHANOL EXTRACT SINGLE BULB BLACK GARLIC AS AN ANTIHYPERURICEMIC IN RATS Abdul Aziz Setiawan; Shirly Kumala; Dian Ratih L.; Nancy Dewi Yuliana; Rochimah; Saru Noliqo Rangkuti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 2 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i2.755

Abstract

Black garlic tunggal (BG) adalah produk fermentasi bawang putih tunggal pada suhu, dan kelembaban terkontrol selama 29 hari, sehingga mengalami reaksi Maillard. Penelitian terkait efektivitas antihiperurisemia BG belum pernah dilakukan. Penelitian ini bertujuan untuk mengetahui efektivitas antihiperurisemia dari ekstrak metanol 80% BG. Metodologi penelitian bersifat eksperimental berupa studi in vivo, hewan uji yang digunakan yaitu tikus putih jantan galur Sprague Dawley, dibagi menjadi 6 kelompok, kontrol normal, kontrol negatif, kontrol positif (Allopurinol 27 mg/kg BB tikus), kelompok perlakuan ekstrak metanol 80% BG dosis 62,5 mg/kg BB, 125 mg/kg BB, dan 187,5 mg/kg BB tikus. Sampel uji dan kontrol diberikan secara peroral selama 7 hari berturut-turut, lalu hari ke-8 diinduksi kalium oksonat secara intraperitonial dan pengambilan darah melalui sinus orbital, kemudian dilakukan pengujian dan dianalisis dengan oneway ANOVA. Hasil penelitian menunjukkan kelompok kontrol positif dan perlakuan I, II, III ekstrak metanol 80% black garlic tunggal mempunyai rata-rata sebesar 2,63; 3,99; 3,60 dan 2,75 mg/dl. Hasil Post Hoc LSD menunjukkan kelompok kontrol positif tidak memiliki perbedaan bermakna dengan kontrol normal dan ekstrak metanol 80% BG dosis 187,5 mg/kg BB tikus. Kesimpulan penelitian ini adalah studi in vivo ekstrak metanol 80% BG hari ke-29 mempunyai efektivitas sebagai antihiperurisemia pada tikus galur Sprague dawley. Kata Kunci: Black garlic tunggal, persen kadar asam urat, antihiperurisemia
Hubungan Rasionalitas Penggunaan Antibiotik Terhadap Outcome Klinik Pasien Pneumonia Komunitas Rawat Inap di RSUD. Dr. H. Abdul Moeloek Provinsi Lampung Tahun 2019 Widiyastuti, Asih; Kumala, Shirly; Utami R, Hesty; Pratama, Anton
Jurnal Kesehatan Vol 14 No 1 (2023): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v14i1.3483

Abstract

Pneumonia is a lung parenchyma disease caused by bacteria, fungi, viruses, and parasites. Irrational use of antibiotics will lead to antibiotic resistance, so the goal of therapy has yet to be achieved. This study evaluates the rationality of using community antibiotics for the clinical outcome of community pneumonia patients in RSUD. Dr. H. Abdul Moeloek.This research is descriptive-analytic with a cross-sectional design and retrospective data retrieval from medical records in 2019. The data analysis was carried out descriptively, and the correlation analysis of the rationale of antibiotic use with clinical outcomes using the Spearman test. The study's results of 144 analyzed showed the characteristics of community pneumonia patients, 78 male, and 66 female patients. The evaluation of the use of antibiotics using the ATC/DDD method showed that Ceftriaxon DDD was 46.80 DDD/100 patient-days, Azithromycin (23.91 DDD/100 patient-days), Levofloxacin (22.70 DDD/100 patient-days). With the Gyssen method, patients used antibiotics correctly (category 0) by 72.91% and incorrectly (categories I – VI) by 27.09%. Statistical analysis showed a significant relationship (p-value=0.003) between the rational use of antibiotics and patient clinical outcomes. Further research is needed regarding the incidence of Drugs Related Problems on the clinical effects of community pneumonia patients.
Colocasia esculenta L. Extract Increase Growth and Motility of Rotenone Induced Zebrafish (Danio rerio) Larvae Stunting Model Furqon, Muhammad; Kumala, Shirly; Farida, Yunahara; Zaidan, Sarah; Sumiyati, Yati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10670

Abstract

This study aims to determine the effect of Colocasia esculenta L on the growth and motility of zebrafish (Danio rerio) larvae after induction of rotenone as a stunting model. The Zebrafish have divided 3 groups i.e Normal (embryonic medium without rotenone induction), Negative Control (embryonic medium with rotenone induction), and Treatment Groups (embryonic medium with rotenone induction and Colocasia esculenta extracts, dose 30-60-90 ppm). The stunting model is designed by induction of 12.5 ppb rotenone to Zebrafish larvae. The growth of the larvae was observed by using a microscope to measure the body and head length. Evaluation of motility was carried out by recording the movement of the fish for 1 minute, and the pace was analyzed by using Video Tracker. The evaluations were observed at 3, 6, 9 dpf (day post-fertilization). Colocasia Esculenta Extract (CEE) groups have higher body lengths compared to the Negative Control Group. The lengths were 125.4 µm for CEE30, 119.4 µm for CEE60, and 121.4 µm CEE90 while the length for the Negative Control Group was 118.3 µm at 9 dpf. However, there were no different values for the ratio of head length to body length of all groups. The ratio was 1:6 at 3 dpf, and 1:4 at 6 and 9 dpf. Moreover, the motility was higher in CEE groups compared to the Negative Control at 3, 6, 9 dpf. The body length and motility of Zebrafish larvae higher compare to the Negative Control Group showing the potential of Colocasia esculenta extract on growth and enhancement of motoric activity.
Freezed Drying of Kelor Leaves Extract (Moringa oleifera Lam.) Yonathan Tri Atmodjo Reubun; Shirly Kumala; Siswa Setyahadi; Partomuan Simanjuntak
Jurnal Sains dan Kesehatan Vol. 3 No. 4 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i4.427

Abstract

Moringa oleifera Lam. is a plant that is widely used by people in Indonesia because it is believed to be one of the medicinal plants for various diseases such as diabetes, diuretics, and has the potential to prevent Alzheimer's disease. The purpose of this research is to obtain kelor leaves extract which is more stable in storage and durable, namely by using the freeze drying method, which in this method aims to remove the moisture content in an ingredient or extract. The initial stage of making the extract begins with the extraction of kelor leaves using the maceration method using ethanol as a solvent. The results of maceration are collected and concentrated using a rotary evaporator until a thick extract is obtained and followed by freeze drying which aims to obtain a thick, stable extract. The results showed that 467.12g of kelor leaves extract from 1,000g simplicia was obtained where the yield obtained was 46.71%. the extract yield after freeze drying was 286.03g. After that the extract was tested for phytochemical screening where the results showed that the Moringa leaf extract contained alkaloids, saponins, tannins, phenolics, flavonoids, glycosides, and steroids.
Evaluation of Drug Procurement Pattern and Drug Availability for JKN Patients at X Hospital and Y Hospital, Cirebon District, January-December 2022 Period Siti Jabal Mastura; Prih Sarnianto; Shirly Kumala; Dosi Ahmad Yani; Hery Prambudi
Daengku: Journal of Humanities and Social Sciences Innovation Vol. 4 No. 5 (2024)
Publisher : PT Mattawang Mediatama Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35877/454RI.daengku2569

Abstract

The era of National Health Insurance (JKN) brought changes in the health care system, which required hospital pharmacists to adapt to drug regulations and planning. Procurement planning and procedures have a significant impact on the availability of drugs and the financial situation of the hospital in drug management. One of the most crucial aspects of the hospital is the availability of guaranteed goods and sufficient drug supplies in order to provide the best service. This research was conducted to evaluate the procurement and availability of drugs at X Hospital as a public hospital and Y Hospital as a private hospital in Cirebon Regency. The analysis used in this study is descriptive-evaluative, namely the research method used to obtain data in a descriptive form and a systematic analysis is carried out to evaluate based on the descriptive data. Data collection was carried out by means of in-depth interviews with the hospital and distributors as external parties who also contributed to fulfilling the availability of drugs. The data is also complemented by the results of field observations and review of document reports related to the National Formulary (Fornas), Hospital Formulus (Forkit), and Drug Needs Plan (RKO). The results of this study found that the Drug Needs Plan, procurement and availability of drugs at X Hospital and Y Hospital were in accordance with the standards. This shows that most of the drugs available and given to patients are in accordance with the drugs listed in the National Formulary. There is a difference in the pattern of procurement. Hospitals conduct procurement twice a month, but private hospitals conduct procurement patterns twice a week. The two hospitals both use the SIMRS and Teramedik management information systems for the evaluation process of drug procurement and availability
Toxicity of Sida rhombifolia L. 96% ethanol extract based on LD50 and macropathological examination of mice's organs Aulena, Desi Nadya; Kumala, Shirly; Abdillah, Syamsudin; Rahmat, Deni; Zaidan, Sarah; Fitriyani, Dwi; Raihan, Dany
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 1 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i1.1463

Abstract

The Indonesian people empirically use Sidaguri (Sida rhombifolia L.) in medicine, such as anti-hyperuricemia. Herbal-based treatment is currently much preferred. Drug metabolism, in general, mainly occurs in the liver, so the possibility of damage to this organ is high. This study aims to determine the acute toxicity category of 96% ethanol extract of sidaguri based on the LD50 value, changes in body weight, changes in organ weight, and macro pathology in the organs of male DDY mice in vivo. The method used in this research was experimental with a fixed dose design following the Indonesian Food and Drug Authority regulations regarding guidelines for in vivo non-clinical toxicity tests. The test group was divided into six dose groups (standard, 50, 300, 2000, 5000, and 15000 mg/kg Body of weight). Observations were made for 24 hours. Observations were continued for 14 days on death parameters, toxicity symptoms, body weight, and relative organ weights using five main organs (heart, lungs, liver, spleen, and kidneys, and pathological examination). The acute toxicity test results showed no death in all dose treatment groups. Macropathological analysis did not show abnormalities in organs in all groups of mice. LD50 value is more than 15000 mg/kg. Sidaguri 96% ethanol extract is safe and Practically non-toxic.
Branded Drug Planning Based on (Combination) of Consumption Methods With ABC Analysis and Reorder Point (ROP) its Effect on Inventory Value and Turn Over Ratio (TOR) in the Pharmacy Installation of X Hospital, Bekasi Muhammad Sapuan; Shirly Kumala; Sri Widyastuti
Jurnal Info Sains : Informatika dan Sains Vol. 15 No. 01 (2025): Informatika dan Sains , 2025
Publisher : SEAN Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Planning drug needs in the Pharmacy Installation at Hospital X Bekasi, using the consumption method. This causes shortages and excess stock of branded drugs so that the drugs are not used and expire. The high value of branded medicine inventory at Hospital The aim of this research is to determine the difference in inventory value and Turn Over Ratio (TOR) of fast moving branded drugs after testing the application of a branded drug planning model based on a combination of consumption methods with ABC analysis and reorder points compared to the planning before testing in Hospital Pharmacy Installations. X Bekasi. The type of research carried out was pre-experimental using analytical descriptive methods. The approach used in this research is cross sectional. In-depth interviews and Focus Group Discussions were also conducted. The trial was carried out on fast moving ABC branded drugs for 3 months, namely June-August 2023. The quantitative approach was by calculating the inventory value and TOR value of fast moving group A, B and C branded drugs, before and after the trial. The research results prove that the application of model trials can reduce the inventory value of ABC fast moving branded drugs from IDR 124,848,282 to IDR 122,454,522, increase the TOR of ABC fast moving branded drugs from 5.66 to 8.12 and obtain an efficiency of IDR 2,393,760. The conclusion of this research is that planning for fast moving ABC branded drugs based on a combination of consumption methods with ABC and ROP analysis has been proven to reduce inventory value and increase TOR and obtain an efficiency of 1.92%.
Optimalisasi Lembaga Farmasi TNI AL Dalam Produksi Sediaan Farmasi Guna Mendukung Ketahanan Kesehatan Ruskar, Dandung; Kumala, Shirly; Noor, Laili Savitri
PendIPA Journal of Science Education Vol 9 No 1 (2025): February
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.1.132-139

Abstract

RIPIN (National Industrial Development Master Plan) 2015-2035 has placed the Pharmaceutical Industry as one of the priority industries to be developed. Meanwhile, the results of the ASEAN Summit in September 2023 held in Jakarta stated that Indonesia had the lowest score for indicators of drug availability and costs, which was 54.02. The existence of BPJS Kesehatan in 2014 greatly reduced the role and function of the Navy Pharmaceutical Institution as the pharmaceutical industry owned by the TNI AL. Currently, Lafial only produces pharmaceutical preparations for health support whose need is very limited because the state budget received is also reduced. In order to support and increase the availability of medicines in Indonesia, the role and function of Lafial should be utilized. The purpose of this study was to design the right business strategy for Lafial to meet the needs of national medicines while reducing dependence on the state budget through SWOT analysis and QSPM matrix. This was qualitative research using a descriptive analysis approach to further explore the internal and external parameters factors of Lafial. The data collection technique used was triangulation from in-depth interviews and FGDs on key personnel pharmacists as well as questionnaires through Google Forms for all pharmacists outside key personnel and secondary data collection. The results of the study on the strength component score was 2.022; the weakness component was 1.540; the opportunity component was 2.020 and the threat component was 1.673. With these results, Lafial's position is in cell number I of the IE matrix and quadrant I of the SWOT quadrant. The strategy analysis using QSPM provides that the strategy of sharpening market penetration through coordination with the Officials and Heads of the Navy health facilities to use Lafial's finished products has the highest TAS value with a value of 5,611. Then the strategy of developing the market through coordination with the Ministry of Health and the Ministry of Finance to be able to produce and deposit PNBP has a TAS value of 5.204. The last strategy is a strategy to increase operational efficiency through coordination with the state-owned and private pharmaceutical industry through drug toll manufacturing activities with a TAS value of 4.332.
In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum Aji, Nur; Kumala, Shirly; Mumpuni, Esti; Rahmat, Deni
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.  
Antibacterial Activity of Kecombrang (Etlingera elatior) Stems Against Skin Infection-Causing Bacteria Adini, Syilvi; Kumala, Shirly; Setyahadi, Siswa
Sciences of Pharmacy Volume 4 Issue 3
Publisher : ETFLIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0403322

Abstract

Skin infections caused by Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes often exhibit resistance to conventional treatments. This issue has led to the exploration of medical plants, such as kecombrang stems (Etlingera elatior), which are known for their antibacterial properties. This study aimed to evaluate the antibacterial activity of kecombrang stem and to identify its active compounds. The methanolic extract of kecombrang stems was tested against the three bacteria using the disc diffusion method at concentrations of 80%, 40%, and 20%. The Minimum Inhibitory Concentration (MIC) was determined using the microdilution method and an ELISA reader. TLC-Bioautography was employed to identify the antibacterial compounds present in the extract. The methanol extract of kecombrang stems exhibited antibacterial activity against S. aureus, S. epidermidis, and P. acnes, with an inhibition zone diameter ranging from 9.23 ± 0.472 to 25.53 ± 0.378 mm. The MIC results showed that the minimum concentration of 78.12 ppm could inhibit the growth of S. aureus by 33.74%, S. epidermidis by 14.45%, and P. acnes by 3.5%. The results of TLC Bioautography analysis indicate that flavonoids exhibit antibacterial properties. The kecombrang stem has the potential to serve as an antibacterial agent against bacteria that cause skin infections.
Co-Authors . Desi . Suhaimi . Syamsudin . Yuliani Abdul Aziz Setiawan Adefira Elisyia Juniharti Adini, Syilvi Afrini Saraswati Agan Syahrial Agung Dewantoro Ahmad Subhan Aji, Nur AMEILIA AMEILIA Anarisa Budiati Andri Prasetiyo Andria Agusta Andrianopsyah Mas Jaya Putra Anggelina Aprilia Pangalila Anggi Isnaasar Anny Victor Purba Anwar Wahyudi Anwar Wahyudi Anwar, Yelfi Ariyani Kiranasari Ariyani Kiranasari Armansyah, Septiyana Lia Asnah Tarigan Atut Ruswita Atut Ruswita Handayani Aulena, Desi Nadya Aulia Tisna Dewi Ayuditiawati, Meita Bekti, Novi Dyah Purnama Bekti, Novi Dyah Purnama Carrista Carrista Dandung Ruskar Datubara, Martaulina Elisabet Delina Hasan Deni Rahmat Desi Nadya Aulena Devana Devana Dewi Laxmi Diah Dethriani Dian Indriani Dian Ratih L. Dian Ratih Laksmitawati Didik Tulus Didik Tulus Dimas Adrianto Dimas Adrianto Dosi Ahmad Yani Dwi Aryanti, Anindita Dwi Fitriyani, Dwi Dwi Hapsari Juniarti Dwi Windi Sapitri Dwi Windi Sapitri EDY MEIYANTO Elvina Dhiaul Iftitah Elvina Dhiaul Iftitah ENDRO BUDI SISWANTO Erlita Agustina Erlita Erlita Esti Mumpuni Esti Mumpuni Esti, Esti Fachry Abda El Rahman Farida, Yunahara Faridah Faridah Fauzi Setyo Fandheo Fauzia Noprima Okta Febbyasi Megawaty Gabriel Gabriel Gabriel, Gabriel Gandes Winarni Gumilar Adhi Nugroho Halim, Marta Hasan, Delina Herru Setiawan Hersunaryati, Yetti Heru Santosa Heru Santosa Hery Prambudi Hesty Utami Hesty Utami Hesty Utami R Huda, Badrul I Ketut Suada I. G.A. Ayu Wiratih Iha Haryani Hatta Iha Haryani Hatta Indah Masri Indarwati Indarwati Khairunnisa Tamimi Kurota Aini Laili Savitri Noor Lea P. Retnoningsih Leonardus B.S. Kardono Lestari Rahayu Lies Putriana Lilik Sulastri Mardiastuti, . Mardiastuti, . Marlin M. Raunsai Meiyanto, Edy Meva Sari Chandra Miftahurrohmah, Nur Mochtar, Dede Mochtar, Dede Mulatsari, Esti Muthi Ikawati Nancy Dewi Yuliana Nancy Dewi Yuliana Nancy Raisa Nancy Raisa, Nancy Noor, Laili Savitri Noviyantih, Noviyantih Nur Aji NUR ANNISA FITRI Nurmaini Nurmaini Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Pasanema, Dimas A. M. Pasanema, Dimas A. M. Pasaribu, Rita Kristina Peny Budiarti Pieter Hazmen Pratama, Anton Pratiwi Pujilestari Sudarmono Prih Sarnianto Prih Sarnianto Priyo Wahyudi Qodriah, Rahmatul R, Hesty Utami Rachmani, Rum Rachmani, Rum RAHMAT, DENI Raihan, Dany Ramadaniati, Hesty Utami Raymond R. Tjandrawinata Reise Manninda Restu Restiani Reubun, Yonathan Tri Atmodjo Reza Agung Sriwijaya RIRIS ISTIGHFARI JENIE Risma Marisi Tambunan, Risma Marisi Rita Kristina Pasaribu Robert Utji Rochimah Ros Sumarny RR. Ella Evrita Hestiandari Ruskar, Dandung S.Farm., M.Farm., Apt, Sondang Khairani Sarah Zaidan Sarnianto, Prih Saru Noliqo Rangkuti Sembiring, Rinawati Septisetyani, Endah Puji Sesilia A. Keban Sesilia Andriani Keban Sesilia Andriani Keban Setiadi, Feri Setiawan, Abdul Aziz Setiyarini, Setiyarini Shanny, Fransisca Shanny, Fransisca Simanjuntak, Partomuan Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siswa Setyahadi Siti Jabal Mastura Sofyan Ramani Sri Widyastuti Stiani, Sofi Nurmay Sugiastuti, Fenty Suhaimi Suhaimi Suhaimi, . Syamsudin Syamsudin Syarifah Aini Syarmalina Syarmalina Teti Indrawati Teti Indrawati Umilawati Rawi Utami R, Hesty Violeta, Violeta Vyacheslav Dushenkov Wahyudi Uun Wibowo Mangunwardoyo Wida Feriningsih Widiyastuti, Asih Yati Sumiyati, Yati Yetti Hersunaryati Yonathan Atmodjo Reubun Yun Astuti Nugroho Yuniarto, Ari Yusransyah, Yusransyah