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All Journal Majalah Obat Tradisional Universa Medicina Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Medik Veteriner JPSCR : Journal of Pharmaceutical Science and Clinical Research Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Fisheries & Marine Jurnal Widya Medika
Kuncorojakti, Suryo
Program Studi Sarjana Kedokteran Hewan Fakultas Kedokteran Hewan Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Surabaya, Indonesia 60115.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Universa Medicina

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Rapid isolation and characterization of Wharton's jelly-derived mesenchymal stem cells maintained in fresh-prepared human AB-serum Tjahjono, Yudy; Dewi, Sianty; Novita, Bernadette Dian; Wijaya, Hendy; Putra, Brilliant Dwi; Kuncorojakti, Suryo; Hendriati, Lucia; Jong, FX Himawan Haryanto; Widodo, Teguh; Malonda, Franklin Vincentius
Universa Medicina Vol. 44 No. 1 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.65-72

Abstract

Background Mesenchymal stem cells (MSCs) are valued in regenerative medicine for their multipotency, proliferative capacity, and immunomodulatory properties. Wharton’s jelly-derived MSCs (WJ-MSCs) from the umbilical cord offer a non-invasive, promising source for clinical applications, because easy isolation, lack of ethical concerns, and the presence of both embryonic and adult stem cells have made them a valuable source for use in therapeutic applications and regenerative medicine. This study aimed to optimize WJ-MSC isolation and characterization methods. Methods Human umbilical cords from three healthy donors were collected post-cesarean under strict inclusion criteria. WJ-MSCs were isolated using the explant culture method, with cells adhering to T75 flasks pre-coated with 2% gelatin. Cultures were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% freshly prepared Human AB serum and monitored for 21 days. Flow cytometry (BD FACSAria) was performed at passages 1 and 5 to assess MSC markers CD105, CD73, CD90, and CD44, alongside the exclusion marker CD45. Results WJ-MSCs exhibited fibroblast-like morphology by passage 1 and showed robust proliferation. Flow cytometry revealed high CD44 expression (~60%) at passage 1, while CD105, CD73, and CD90 became prominent by passage 5. CD45 remained low, suggesting minimal hematopoietic contamination. Conclusion This study confirms the feasibility of isolating and expanding WJ-MSCs using DMEM with 10% human AB serum. While consistent cell growth was achieved, the 21-day culture period may require optimization for scalability, including serum concentration, substrate coatings, and oxygen levels. CPJ-MSCs may be preferable for applications demanding rapid expansion and early marker expression.
Co-Authors A'la, Rofiqul Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Annas Salleh Annise Proboningrat Arimbi Aswin, Ahmad Bambang Sektiari Lukiswanto Boedi Setiawan Chozin, Aminullah Alhady Chusniati, Sri Daruti Dinda Nindarwi Delaiah, Diena Dewi, Nina Nurmalia Dita Wisudyawati Diyantoro Diyantoro, Diyantoro Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Fecky Fernando Fredericktho Fedik Abdul Rantam Gazali Salim Hani Plumeriastuti Helen Susilowati, Helen Hendrata, Adi Pramono Heny Kristi Meitasari Hidayatik, Nanik Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Jaya, Ferdinand Wiliam Jessica Giovani Jong, FX Himawan Haryanto Khairullah, Aswin Rafif Legowo, Djoko LINA SUSANTI Lucia Hendriati Lucia Hendriati Lucia Tri Suwanti, Lucia Tri Malonda, Franklin Vincentius Martha Ervina Maulana, Firdausy Kurnia Mirny Lamid Mohammad Noor Amal Azmai Muchammad Yunus Ni Putu Pirna Wijayanti Novita, Bernadette Dian Nusdianto Triakoso Paul L Tahalele Prastiya, Ragil Angga Putra, Brilliant Dwi Putri Desi Wulan Sari Putri, Mirtanti Kiswandi Rahmahani, Jola Rao, Sherbrina Bai Seenivasa Rendragraha, Briantono Willy Rodprasert, Watchareewan Rozi Santanumurti, Muhammad Browijoyo Santoso, Kuncoro Puguh Setiawan Koesdarto Sharveihn Sathindren Sianty Dewi Soediono, Endang Isbandiati Sukmanadi, Mohammad Sumi WIjaya, Sumi Susilawati, Helen Suwandito, Laurensius Suwarno Suwarno Suzanita Utama Teguh Widodo Teguh Widodo Teguh Widodo Teguh Widodo Tita Damayanti Lestari Tjahjono, Yudy Wedharga, I Gede Putu Adhi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wijaya, Andi Yasmin Wijaya, Hendy Wilis Prasasti Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Yeni Dhamayanti Yulianna Puspitasari Yunita, Maya Nurwartanti