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Journal : Animal Production : Indonesian Journal of Animal Production

g.640T>C Polymorphism of the TGF-β2 Gene is Associated with Salmonella pullorum Resistance in Indonesian Chickens Muhsinin, Muhammad; Ulupi, Niken; Gunawan, Asep; Wibawan, I Wayan Teguh; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1109.044 KB) | DOI: 10.20884/1.jap.2017.19.2.597

Abstract

The objectives of this study were to identify polymorphism of transforming growth factor β2 (TGF-β2) gene associated with Salmonella pullorum resistance in Indonesian chickens. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype Indonesian chickens. Analysis of polymorphism was conducted by using PopGen 3.2 software. The effect of genotype on S. pullorum resistance was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 278 chickens from 7 Indonesian chicken populations (Sentul, Merawang, Pelung, Kampung, Parent Cobb broiler, The F1 crossbreed of Kampung x Parent Cobb broiler (KB) and F2 KB x KB. The product of amplification was 284 bp. The TGF-β2| RsaI locus was polymorphic in all populations, producing two alleles (T and C) and three genotypes (TT, CT, and CC). The result from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the C allele in all populations. The χ2 analysis showed that the 6 chicken populations were deviated from Hardy-Weinberg equilibrium, with the exception of the Parent Cobb broiler chicken. The association result showed that TT genotype was significantly associated with S. pullorum resistance in Sentul chicken. Although the leukocyte concentration, leukocyte differentiation and H/L ratio in Sentul chicken with three of TGF-β2 genotypes (TT, TC, and CC) were not statistically different. In conclusion, polymorphism in the TGF-β2 chicken gene can be used as a candidate marker to increase S. pullorum immune response.
Identification of poymorphism and association analyses of FMO3 gene related with carcass and meat quality in Cihateup Duck Anggraeni, Anggraeni; Gunawan, Asep; Rukmiasih, Rukmiasih; Suryati, Tuti; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 3 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (812.634 KB) | DOI: 10.20884/1.jap.2017.19.3.623

Abstract

The aim of this study was to identifypolymorphism and association of the FMO3 gene related to carcass and meat quality in Indonesian Cihateup ducks. A total of sixty Indonesian Cihateup ducks were used in this study. Tissues from breast muscleswas used for genomic DNA. Association analysis showed that the SNP g.849A>G was highly significantly associated (P<0.01) with live weight (LW), carcass weight (CW), breast muscle weight  (BMW), pH, cooking loss, drip loss (DL), lightness (L*) and redness (a*), TBARS and TMA. Compared to the GG genotype, the AG genotype exhibited greater levels (P < 0.05) LW, CW, pH, a*, TBARS and TMA but not DL and L*.  These results will improve the understanding of the functions of the FMO3 gene in carcass and meat quality within the liver and will shed light on FMO3 as a candidate gene in the selection of ducks with good carcass and meat quality traits
Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep Harahap, Ratna Sholatia; Noor, Ronny Rachman; Gunawan, Asep
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.88

Abstract

HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fat-tailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.
Co-Authors . Nurjanah . Uju Abdul Salam Adil Adil, Adil Afdilla, Dian Lailisa Afkar Rona Indarwati Afrianto Daud Agoes M. Jacoeb Agoes Mardiono Jacoeb Agung Prabowo Agus Oman Sudrajat Agy, Sarah AHMAD ENDANG ZAINAL HASAN Ahmad Syihan Ismail Aisyah Fatriani Alan Dwi Irawan Amalia, Lisya Fiani Amanda, Amelia Amang Sudarsono, Amang AMIN FATONI AMINUDDIN KASDI Anggraeni Anggraeni Aninditya Artina Setiaputri Anita Maulina Anjar Nurrohmah Annas Wiguno Anneke Anggraeni Annisa Annisa Apodekta, Astrid Antheosiaaretes Apon Zaenal Mustopa Apon Zaenal Mustopa Apriana Vinasyiam Ari Wawasto Arlita, Kriswidya Aryana, I.B.Putra Manik Asadatun Abdullah Asmarasari, Santiananda Arta Astuti, Ida Puji Aulia Sekar Sari Ayu Ratih Purnamasari Azizah Nuraini Azizah, Septiana Nur Cece Sumantri Chaidir Chaidir Chevy Kusumah Wardhana Cindytia Prastari Damayanti, Tiwi Dase Hunaefi Deden Yusman Maulid Dewi Puspitasari Diah Savitri E Dian Rianita Didik Gunawan Tamtomo Diini Fithriani Dini Nurfianti Dita Mirawati Dreyfus, Shoshana Dwi Setyaningtyas Dwi Susilaningsih DWI SUSILANINGSIH Dwiwahyuni, Pricillia Dyah Prasetiani Dyah Rahmawatie Ratna Budi Utami Effionora Anwar Effionora Anwar ekamaida, ekamaida Eko Fajar Nurprasetyo Ekowati Chasanah Eliwarti Eliwarti Endang Sri Wahyuni Endang Supriyadi Endang Supriyadi Epi Taufik Erin Apriliani Wulandari Putri Ernik Yuliana Eva Banowati EVI MAYA SARI Ewi Pasaribu Faesal A Maodah Fahriza Sri Afni Fahrury Romdendine, Muhammad Farida Hanum Farida Isroani Fatriani, Rizka Febriana, Suci Wulan Fitri, Nurul Fitria Tika Pradita Ganjar Samudro Gede Wibawa Gerry Immanuel Tanius Giri Rohmad Barokah GuoFeng Wang Gusti Hardiansyah Hafit Isandono Hana Aulia Sativa Handayani, Indah Widuri Hanifah Husein Hizbullah Hanifaturahmah, Fadiyah Hasibuan, Lailan Sahrina Hawis H Madduppa Heidi Wiratmaja Hilda Lu’lu’in Nanda Alfira Devi Huda Fajar Arianto HUTASOIT, NICOLAS I Gede Puja Astawa I MADE ARTIKA I Nyoman Sujana I wayan Teguh Wibawan Iis Diatin Ika Krisdiana Ika Silvitasari Ikromatun Nafsiyah Ima Hani Setiawati Indarwati Indarwati Irawaty Irawaty Irfan Suliansyah Irmawati Irmawati Is Mardianto Iskandar AM, . Iskandar, Maghfirany Israyani Istiqomah, Anjula Roselini Isworo, Rhestu Isworo, Rhestu Jaeng, Maxinus Jamaluddin Jamaluddin Jenni, Jenni Joko Hermanianto Joko Santoso Juandy Seiver Langelo Karjunita, Nike Karunia, Dede Khaswar Syamsu Kinanti Permata Sitaresmi Komalasari, Titin Kukuh,I Kd, Angga Kurniasari, Marlinda Kurniawan, Kito Kustiariyah Tarman Kusumawati, Rizky Lahming, Lahming Lilis Trisnawati Lily Viruly Linawati Linawati M. Fedi A. Sondita Maemonah, Maemonah MAGGY T. SUHARTONO Maggy T. Suhartono Maggy T. Suhartono Mahardika, Vania Mahyudi Mahyudi Majariana Krisanti Manalu, Lamhot Mandarin, Dewi maryatun maryatun medal lintas perceka Mega Andini Mega Andini Mega Safithri Mia Setiawati Mita Wahyuni Mohamad Taufik Mohammad Mukhlis Kamal Mohammad Nasichin Muh Tazri Nasirullah Muhamad Fedi Alfiadi Sondita Muhammad Baiquni Bramantyo Muhammad Jufri Muhammad Sujatmiko Muhsinin, Muhammad Mulyadi, Yulius Daniel Mulyati Mulyati Munandar, Aris Mustarin, Amira N. Nurjanah N. Nurjanah Nadia Fabella Neng Dewi Himayasari Netti Herawati Niken Ulupi Nini Marta Nofiarli Nofiarli Norita Norita Novi Luthfiyana Novia Nanda Saputri Novita Sari Noviyan Darmawan Nur Azizah Nasution Nur Mahmudi Ismail Nuratriningrum, Ari Nurdevi Noviana Nurfajrin Nisa Nuri Andarwulan Nuring Wulansari Nurjanah Nurjanah . Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjumadil, Muhammad Nurlisa Alias Butet P.K. Dewi Hayati Panca Jarot Santoso Pertiwi, Rizsa Mustika Pipih Suptijah Prabawati, Chintya Priyo Hadi Purnawati Purnawati Puspita Sari Dewi Puspita, Riya Rahadian Hardja Utama Rahayu, Pravita Rahma Dini Arbajayanti Rahma Dini Arbajayanti Ratih Dewanti -Hariyadi Ratna Sholatia Harahap Restu Lusiana Retno Purnama Irawati Reza Febriyansyah Rhestu Isworo Ria Riski Marsuki RIDWAN AFFANDI Rieska Roskananda Rina Sri Widayati Rina Supriatnaningsih Rismawanti, Erma Ristika Handarini Rita Khairina Rita Wahyuni Riyani Wulandari Rizky Tetriyanda Rizsa Mustika Pertiwi Rizsa Mustika Pertiwi Rizsa Mustika Pertiwi Rizsa Mustika Pertiwi Rofi Ahmad Dzaky Roni Nugraha Ronny I. Wahju Ronny Rachman Noor Rosmawati Peranginangin Roza Yusfiandayani Rukmiasih Rukmiasih Sabaria Manti Battung Safitri, Novemi Gita Sahaba, Muh. Alsere Bardian Saktiansyah, La Ode Ahmad Salham, Munir Salsabila, Fitria Dewi Samiruddin T, Samiruddin T Sapja Anantanyu, Sapja Sari, Karisma Diana Sarina Sarina SEDARNAWATI YASNI Setiyo, Septian Adi Sholekhah, Hanif Zulfahmi Shugo Watabe Sigid Hariyadi Siregar, Sheyra Silvia Siti Julaeha, Siti Solly Aryza Sri Purwaningsih Sri Rejeki Urip Sri Sumiyati Srikandi, Louise Dwi Kumala Stevi Imelda Murniati Wodi Stevy Imelda Murniati Wodi Suhadi Suhadi Sukatmadiredja, R Noneng Surya Dwipangga Susie Chrismalia Garnida Sutoyo Sutoyo SYAHRIAL SYAHRIAL Syamsul Hadi T. Suryati Tatag Budiardi TATI NURHAYATI Taufik Hidayat Taufik Hidayat Taufik Hidayat Taufik Hidayat Titi Candra Sunarti Titin Martini Tuty Hardianti Uswatun Hasanah Uyunun Uyunun Vicentius Marco Matutina Wardani, Septiana Dewi Warsono Warsono Widiyanto Widiyanto Wildan, Dudi Muhammad Wini - Trilaksani Wisnu Ananta Kusuma Yanti Sinaga Yanti, Rukmana Yayi Suryo Prabandari Yoshihiro Ochiai Yugha Subagja Yuriyandhi, Yuriyandhi Zaim Elmubarok ZK Abdurahman Baizal Zumrotussholikhah, Annisa’