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Journal : International Journal Of Science, Technology

Phytochemical Screening And Antibacterial Activity Of The Ethanol Extract Of Putri Malu (Mimosa Pudica Linn.) Leaves Combined With Klanceng Honey Against Staphylococcus Aureus Bacteria Dalimunte, Khairunnisa; Arianto, Anayanti; Salim, Emil
International Journal of Science, Technology & Management Vol. 4 No. 6 (2023): November 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i6.965

Abstract

Mimosa pudica Linn. Leave and honey have been known to have antibacterial activity. The combination of the two is very beneficial as an antibacterial because the hydrogen peroxide content in honey and the flavonoid metabolite compounds in Putri Malu provide a synergistic effect as an antibacterial. The aim of this research was to determine the activity of the ethanol extract of Putri Malu leaves combined with Klanceng honey using the disc diffusion method against Staphylococcus aureus bacteria. The results showed that the resulting extract was thick and blackish-green in colour, with a yield of 25.6%. The results of the phytochemical screening test showed that Putri Malu extract contains alkaloids, flavonoids, glycosides, saponins, and tannins. Characterization results showed that the water content of the extract was 7.28 ± 0.05, water-soluble essence content was 16.03 ± 0.08, ethanol-soluble essence content was 19.65 ± 0.01, total ash content was 4.70 ± 0.00, and essence content acid soluble was 0.17±0.02. Antibacterial testing showed that the best ratio of Putri Malu leaf extract to honey to inhibit Staphylococcus aureus was 9:1, with an inhibitory value of 13.60 ± 0.40 mm. In conclusion, the combination of Putri Malu leaf extract with honey strongly inhibits the growth of Staphylococcus aureus bacteria.
Antibacterial Activity and Determination of Total Phenol and Flavonoid of Carica papaya L. Ethanol Extract Karo-Karo, Sry Ulina; Arianto, Anayanti; Salim, Emil
International Journal of Science, Technology & Management Vol. 4 No. 1 (2023): January 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i1.738

Abstract

Infectious diseases are Indonesia's most common. Antibiotics are used to treat infections. Medicine needs new antibiotics due to antibiotic resistance. Antibiotics from plants are possible. Papaya leaf carpain alkaloids may be antibacterial. This study determined papaya leaf compound content, flavonoids' total phenol content, and the optimal concentration of papaya leaf ethanol extract that inhibits Staphylococcus aureus and Pseudomonas aeruginosa. The first stages of the research were the maceration of papaya leaf extract with 96% ethanol solvent, the phytochemical screening test (alkaloid, flavonoid, saponin, tannin, and steroid and triterpenoid tests), total phenol and total flavonoids tests, and the paper disc antibacterial activity test. Alkaloids, flavonoids, glycosides, tannins, steroids, and saponins were found in papaya leaf ethanol extract. Papaya leaf ethanol extract has 59.05 mgQE/g flavonoids and 56.80 GAE/g phenols. The antibacterial activity test of papaya leaf ethanol extract measured the diameter of the inhibitory zone on Staphylococcus aureus and Pseudomonas aeruginosa. Alkaloids, flavonoids, glycosides, tannins, steroids, and saponins were found in papaya leaf ethanol extract. Papaya leaf ethanol extract has 59.05 mgQE/g flavonoids and 56.80 GAE/g phenols. Papaya leaf ethanol extract had the best inhibition zone diameter for Staphylococcus aureus at 150 mg/mL (12.85 mm) and Pseudomonas aeruginosa at 150 mg/mL (13.10 mm).
Co-Authors Abbian Akbar, Nouval Abdilla, Fauzan Adlis Santoni Admi Admi Afdal, Afdal Septiawan Agachi, Regan Tisa Aminah Dalimunthe Aminur Aminuyati Amirullah Amrin Amrin Andika Yose, Fuad Andri Andri Angelyne, Metha Anggoro Hendrawan Anisah Fitria Efendi Arianto, Anayanti Aridamuriany D. Lubis Asmaria Putri, Della Baihaki Hijriyan, Thomi BANGUN, HERIANTO Benni Satria Celsi Karmila Sari Charli, Chintya Ones Christine Dwi Hendria Daffa Al-Khairi, Muhammad Daimon Syukri Dalimunte, Khairunnisa De Lux Putra, Effendy Delvi, Nina Gusmala Dewi, Puteri Nigie Kemala Dini Wagini Donda, Donda Dori Mitra Candana Dudut Tanjung Dwi Syukur Phameswari Dwifajri, Muhammad Effendy De Lux Putra, Effendy De Lux Eko Prasetyo, Bayu El Farhani Hasti Habibi Samosir, Fauziah Elfi Amir Elida Mardiah Endang Sugih Arti. Famella Azhara Fausy, Rian Francisco, Yosua Geliz Luh Titisari Gema N. Yanni Geret, Marta Glorya Sihaloho, Angelica Gunawan Nachrawi Hafid Syahputra Hanna Magdalena Simangunsong, Irene Hanum, Tengku Ismanelly Hapzi Ali Harkamsyah Andrianof Heldalia Hendra Saputra Hendra Saputra Henny Sri Wahyuni Herawaty Ginting Heru Kuswanto Husna, Nikmatul Ikhsan Gusti Rilma Ilahi, Fitratul Imelda Yunita Karo-Karo, Sry Ulina Kasmir Tanjung, Kasmir Keliat, Jane Melita Khamsing Keothoumma Kusuma Danny, Faustina Laimnimitr, Napassawan Lusiana Lusiana M. Faisal Muzaki Mai Efdi Mai Efdi Mariadi Mary, Hilda Matlal Fajri Alif Miharja, Marjan Mondra Neldi Muh. Alfito Ramadhan Muhammad Munar Lubis Nabila, Nabila Nadilla , Nia Nadilla, Nia Naja Sakinah, Thara Napitupulu, Pinastiara Nia Nadilla Nika Rahma Yanti Nikmatul Husna NILA PRATIWI, NILA Novita Ayu Permatasari Novizar Nazir Nugraha, Nila Nurhalima, Nurhalima Oki Wijaya Paranita, Darni Pebrian Siregar, Jonathan Prasetyo, Bayu Eko Prayoga, Andre Putri, Della Asmaria Putri, Rhenesa Rachmat Dwi Putranto Rahma Yanti, Nika Rahman, Naufal Rahmayeni Rahmayeni Rahmiana Zein Ramadhan, Muhammad Fikri Ramadhani Manurung, Wahidah Ratnam, Deni Ravena, Radha Refilda Refilda Refinel Refinel - Revansa, Muhammad Nickhole Rifi Iron Akhfifni Rina A.C. Saragih Rini Sadiatmi RISKA ROBIANTO, RISKA Rosmianti, Rosmianti Rozaki, Zuhud Rozalia Rozalia Sari, Silvia Sartika Sartika Shafiyah, Miranda Sijabat, Elinda Juniwati Siregar, Syifa Sabila Yassarah siti wulandari Sivia Sari Suryati Syafrizayanti Syafrizayanti Syukri Syukri Tanaka, Gracelyn Taufik, Muhammad Taufik Theresia Indira Kristanti Tifani Puari, Aninda Tio Putra Wendari Togi Adnan Maruli Sinaga Tree Sabina, Fani Try Nursylla, Rantyta Tuty Anggraini Ulfareksy, Vinna Alea Upita Septiani Utami, Nita Putri Vicky Brama Kumbara Vicky Brama Kumbara Wahyuni, Anisa Tri Wellia, Diana Vanda Yefrida Yefrida Yeni Stiadi Yuandani Yulasmi, Yulasmi Yulia Eka Putri Yulia Fitri Yulia Yulia Yuliasmi, Sri Yulita Indriani Yunnie Trisnawati Zefriyeni, Zefriyeni Zilfa Zilfa Zumaira