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Resilience response to yellow leaf curl disease and identification of resistance gene analogs (RGA) in pepper (Capsicum annuum) Dyan Kusumaning Ayu; Awang Maharijaya; Muhamad Syukur; Sri Hendrastuti Hidayat
Biodiversitas Journal of Biological Diversity Vol. 22 No. 11 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221104

Abstract

Abstract. Ayu DK, Maharijaya A, Syukur M, Hidayat SH. 2021. Resilience response to yellow leaf curl disease and identification of resistance gene analogs (RGA) in pepper (Capsicum annuum). Biodiversitas 22: 4731-4739. Pepper yellow leaf curl disease (PYLCD) caused by infection of Begomovirus is a serious threat to pepper production worldwide. Identification of the resistance gene analogs (RGA) and resilience response of pepper against PYLCD is needed especially for selection resistance genotype. Evaluation of resistance response involving 28 pepper genotypes was carried out through transmission of Begomovirus using whitefly (Bemisia tabaci) as vector. The result showed that IPB C12 and F4-012328-6-3 were potential resistance genotypes. A total of 15 R gene analogs (CaRGA) containing NBS motif, namely CARGA1 to CARGA15, were identified by degenerated PCR amplification and database mining. The alignment of deduced amino acid sequence revealed conservation of subdomains Ploop (GKTT), kinase2 (LVVLDDV), RNBSB/kinase3 (IILTTR) and GLPL. BLASTp analysis indicated that 15 RGA showed high homology at deduced amino acid level with R gene identified such as whitefly resistance protein Mi-1.2, Pvr 9 gene for potyvirus, Begomovirus resistance protein, TRGA15 and RGA 13 for putative late blight resistance. Phylogenetic analysis exhibited that isolated sequences distinguished into CNL-NBS groups. These pepper RGA could be considered as candidate sequences of resistance genes.
Morphological response and genetic variability of four species of chili pepper (Capsicum spp.) under infection of pepper yellow leaf curl virus Tri Wahono Dyah Ayu Sayekti; MUHAMAD SYUKUR; SRI HENDRASTUTI HIDAYAT; AWANG MAHARIJAYA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 11 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221107

Abstract

Abstract. Sayekti TWDA, Syukur M, Hidayat SH, Maharijaya A. 2021. Morphological response and genetic variability of four species of chili pepper (Capsicum spp.) under infection of pepper yellow leaf curl virus. Biodiversitas 22: 4758-4765. Chili pepper has various types and species, but only five known species are commonly used and consumed. Most cultivated chili is susceptible to various plant diseases, one of which is Pepper yellow leaf curl disease (PYLCD) caused by Pepper yellow leaf curl virus (PYLCV) (Begomovirus, Geminiviridae). To control PYLCD, resistant variety assembly is required to prevent virus infection in cultivated plants. From this research, testing on four chili species is expected to provide information regarding the resistance and performance of chili peppers to conditions infected with PYLCV. This study was conducted at Dramaga Bogor, West Java, Indonesia in two experimental units: planting under virus-free conditions (as control) and virus-infected conditions. Each experimental unit was carried out using a single factor Randomized Complete Block Design (RCBD) with three replications. Twenty-nine genotypes of chili pepper were used consisted of four species, including C. annuum, C. frutescens, C. chinense, and C. baccatum. Of the 29 genotypes tested, thirteen genotypes in the resistant, nine genotypes in moderate resistant, two genotypes in moderate susceptible, three genotypes in the Susceptible, and two genotypes in the highly susceptible category. The heritability, genotypic coefficient of variance (GCOV) and phenotypic coefficient of variance (PCOV) value obtained from testing for all characters is high, ranging from 65.16-99.12%, 14.87-82.60%, and 15.77-84.45%, respectively. Most of the genotypes from C. chinense showed good resistance to PYLCV. In general, by considering the category of the resistance level and other characters such as productivity, ‘Jolokia’ (C. chinense), ‘Anies’ (C. annuum) and ‘Bonita’ (C. frutescens) can be ascertained as potential candidate sources of resistance to PYLCV.
Response diversity and traits related to pepper yellow leaf curl disease resilience for resistant plants selection TRI WAHONO DYAH AYU SAYEKTI; MUHAMAD SYUKUR; SRI HENDRASTUTI HIDAYAT; AWANG MAHARIJAYA; SOBIR SOBIR
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240949

Abstract

Abstract. Sayekti TWDA, Syukur M, Hidayat SH, Maharijaya A, Sobir. 2023. Response diversity and traits related to pepper yellow leaf curl disease resilience for resistant plants selection. Biodiversitas 24: 5057-5064. Many chili species (Capsicum spp.) have been used and consumed, among them five species, namely Capsicum annuum, C. frutescens, C. chinense, C. baccatum and C. pubescens, are widely used. Most of these varieties are susceptible to Pepper Yellow Leaf Curl Disease (PYLCD) caused by Pepper yellow leaf curl virus (PYLCV) (Begomovirus, Geminiviridae). To control PYLCD, it is essential to gather resistant plant varieties to effectively shield cultivated plants from viral infections. An effective selection activity related to the target character is needed to get highly productive resistant plants. The objective of this study was to evaluate the relationship between resistance characters to crop production and to obtain appropriate selection criteria to increase the effectiveness of selecting resistant plants with good production. This study was conducted at Cikabayan Field, IPB University, Bogor, Indonesia. Twenty-nine genotypes of chili pepper were used, which consists of four species, including C. annuum, C. frutescens, C. chinense, and C. baccatum, arranged in a nested block design with three replications. The observed symptoms were yellowing of leaves followed by leaf malformations, but there were differences in the yellowing symptoms. From observation on visual symptoms, resistance characteristics, plant performance and productivity, there were differences in the response of each species to PYLCV attack. Correlation and path analysis results show that there was also differences in performance drop between C. annuum species and the non-C. annuum group. In C. frutescens, C. chinense, and C. baccatum, direct selection on resistance characters was able to increase the resistance level of selected plants while maintaining good crop production. Whereas for C.  annuum species, the plant resistance level was not correlate with productivity reduction, so in the selection process, the percentage of yield decline needs to be considered to obtain resistant plants and maintain crop production.
Performance and yield stability of cayenne peppers (Capsicum frutescens) in multilocation trials in the Indonesian lowlands TRI WAHONO DYAH AYU SAYEKTI; MUHAMAD SYUKUR; SRI HENDRASTUTI HIDAYAT; AWANG MAHARIJAYA; SOBIR SOBIR; KARLIN AGUSTINA; EVRIANI MAREZA; ANUNG WAHYUDI; BAMBANG SUPRIYANTA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 7 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250722

Abstract

Abstract. Sayekti TWDA, Syukur M, Hidayat SH, Maharijaya A, Sobir, Agustina K, Mareza E, Wahyudi A, Supriyanta B. 2024. Performance and yield stability of cayenne peppers (Capsicum frutescens) in multilocation trials in the Indonesian lowlands. Biodiversitas 25: 3008-3017. Cayenne pepper (Capsicum frutescens L.) plant breeding is becoming an increasingly interesting topic due to various usages. The diversity of uses of chilies needs to be balanced with high production and good product quality so that it can meet market needs. This experiment aimed to evaluate the performance and plant diversity of eight elite lines and six commercial varieties of cayenne pepper (C. frutescens) in three different environments. The development of location-specific superior genotypes can be guided to create varieties suited to the targeted environment. This study was conducted in three locations (environments) namely “Lampung”, “Yogyakarta” and “Palembang”. The experiment was conducted using a Randomized Complete Block Design (RCBD) with three replications for each location. Analysis of yield stability was conducted using three methods, namely “Francis-Kanneberg”, “Finlay-Wilkinson” and “AMMI” methods. Among the three locations, the Lampung location experienced a very significant yield loss. This is thought to be due to disease attacks on the plantations. Some genotypes are more suitable for planting at one of the test locations compared to the other two locations. However, there are two genotypes that are stable at three locations based on the three analysis methods used. Genotypes "F1.323340" and "F1.285290” were observed to be stable and produced well at all test locations. Both genotypes can be recommended for planting in lowland environment.
Pengaruh Cekaman Kekeringan terhadap Pertumbuhan Vegetatif dan Keparahan Penyakit Bercak Daun Curvularia oryzae pada Bibit Kelapa Sawit (Elaeis guineensis) Hari Priwiratama; Eka Wijayanti; Suryo Wiyono; Efi Toding Tondok; Sri Hendrastuti Hidayat; Sri Wening
Jurnal Penelitian Kelapa Sawit Vol 33 No 2 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i2.308

Abstract

Drought stress is one of the factors that may influence the growth of oil palm seedlings. In long term, drought can potentially increase the susceptibility of oil palm to plant diseases. This study aims to analyze the effect of drought on vegetative growth and the severity of Curvularia leaf spot disease. Drought stress was simulated by limiting water application to three-month-old oil palm seedlings at field capacity (KL), 75% KL, 50% KL, and 25% KL levels. In each treatment, inoculation of Curvularia oryzae was conducted by spraying a spore suspension (1 x 105 conidia/mL) evenly on the leaf surface. The results showed that infected seedlings under severe drought stress (25% KL) had lower height and crown width compared to treatments KL. The severity of Curvularia leaf spot disease in plants experiencing severe water stress (25% KL) was lower than in the standard nursery treatment, KL, and 75% KL. In general, it can be concluded that drought stress has contrasting effects on plant growth variables and the severity of Curvularia leaf spot disease.
Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF Agus Buono Ali Nurmansyah Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Andika Septiana Suryaningsih Anung Wahyudi Aqlima , Aqlima, nFN Arief Hartono Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Awang Maharijaya Ayu Kartini Parawansa BAMBANG SUPRIYANTA Bambang S. Purwoko Bambang Sapta Purwoko Bayo Alhusaeri Siregar Bib Paruhum Silalahi Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Sukarno Budi Tjahjono Budi Tjahjono Darda Efendi Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti DWI SUBEKTI Dwi Subekti Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Dyan Kusumaning Ayu Efi Toding Tondok Eka Wijayanti Eka Wijayanti Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan EVAN PURNAMA RAMDAN EVRIANI MAREZA Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Giyanto Giyanto Giyanto GIYANTO GIYANTO Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Hari Priwiratama Hari Priwiratama Harti, Heri Harwan Susetio Heri Harti Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Iskandar Zulkarnain Siregar Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas Joni Hidayat, Joni Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumanto Harjosudarmo Jumsu Trisno Kadwati Kadwati KARLIN AGUSTINA Karlisa Priandana Kikin H Mutaqin KIKIN HAMZAH MUTAQIN KRISTIANTO NUGROHO Ladja, Fausiah T. Laksono Trisnantoro LISTIHANI Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Mimi Sutrawati Mubin, Nadzirum Mugi Lestari, Susanti Muh. Taufik Muhamad Syukur Muhammad Herman Muhammad Taufik Muhammad Taufik MUHAMMAD TAUFIK Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI Nurhaelena, Yudia ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri puji Lestari Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat Rahmi Yunianti Rahmi Yunianti Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih RERENSTRADIKA TIZAR TERRYANA Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza RITA NOVERIZA Rita Noveriza Rizqiyah, Sakinah Inayatur Rokhana Faizah Rosmini Rosmini Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto SOBIR Sobir Sobir Sobir Sobir SOBIR SOBIR Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sri Wening Sri Wening Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sugeng Santoso Sumanto Sumanto Supramana Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Tri Puji Priyatno TRI WAHONO DYAH AYU SAYEKTI TRI WAHONO DYAH AYU SAYEKTI Tri Wahono Dyah Ayu Sayekti Tri Wahono Dyah Ayu Sayekti Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani Utomo Kartosuwondo Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo WIDODO WIDODO Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah