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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya El-Hayah : Jurnal Biologi Research Journal of Life Science Jurnal Teknologi Pertanian Berkala Kedokteran MNJ (Malang Neurology Journal) Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Majalah Kesehatan FKUB Health Notions Journal of Dentomaxillofacial Science Jurnal Informasi Kesehatan Indonesia (JIKI) Narra J Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
Hidayat Sujuti
Biochemistry Laboratory, Faculty Of Medicine, University Of Brawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Immunohistochemistry Evaluation of TGF-β1, SOX-9, Type II Collagen and Aggrecan in Cartilage Lesions Treated with Conditioned Medium of Umbilical Cord Mesencyhmal Stem Cells in Wistar Mice (Rattus novergicus) Soetjahjo, Bintang; Hidayat, Mohammad; Sujuti, Hidayat; Fibrianto, Yuda
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.898 KB) | DOI: 10.11594/jtls.08.01.05

Abstract

Currently, umbilical cord mesenchymal stem cells have the potential to be used as treatment options for any cartilage lesion. This research aimed to evaluate the effects of conditioned medium from umbilical cord mesenchymal stem cells (UC-MSC) on damaged cartilage through the expression of proteins TGF-β1, SOX-9, type II collagen and aggrecan, which are known to be related to chondrogenesis. UC-MSC were isolated from 19-days-pregnant Wistar mice and were cultured using the standard procedure to obtain 80% confluence. Subsequently, the culture was confirmed through a microscopic examination that was driven to be an embryoid body to obtain a pre-condition medium. This research utilized 3-month-old male Wistar mice and was categorized into 6 groups (3 control and 3 treatment groups). Each animal had surgery performed to create a femur condyle cartilage defect. The treatment groups were administered a dose of stem cells at 1 mL/kg. Next, immunohistochemical (IHC) staining was performed to examine the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the 2nd, 3rd, and 4th month of evaluation. The results were analyzed statistically using ANOVA test. For each of the treatment groups, there was increased expression (p < 0.05) in all proteins TGF-β1, SOX-9, type II collagen and aggrecan when compared with control groups at the 2nd, 3rd, and 4th month of evaluation. Pre-conditioned medium from UC-MSC potentially increases the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the damaged cartilage of Wistar mice.
α-SMA Expression Increased Over Cell Passages and Decreased by Exogenous TGF-β1, In Vitro Studies on Myofibroblast Derived from Orbital Socket Contracture Shintiya Dewi, Debby; Chairinnisa, Elsa Safira; Sujuti, Hidayat; Lirawati, Diana; Hernowati, Tinny Endang
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.15

Abstract

α-smooth muscle actin (α-SMA), a marker of myofibroblast, induces cytoskeleton reorganization, increases contractility and stimulates cell migration in TGF-β1 induced stress fibers. The aims of the present study were to determine the level of α-SMA expression and morphological cell changes in different passages of myofi-broblasts with varied TGF-β1 concentrations. Myofibroblast cell cultures were derived from fibrotic tissues of fourth degree socket contracture. The α-SMA expression level was measured in myofibroblast cultures pas-sage I, II, and III with and without 10 ng/mL TGF-β1, and in passage III with 2.5; 5; 10; and 20 ng/mL TGF-β1. Results: The levels of α-SMA expression level in passage I to III were I 31.42 ± 3.4; 40.34 ± 8.14 and 56.37 ± 7.57, respectively. Addition of 10 ng/mL TGF-β1 into passage I-III myofibroblast cultures resulted in α-SMA expression level of 31.24 ± 2.93; 36.81 ± 6.09; and 14.29 ± 2.72, respectively. Myoblasts passage III showed the lowest α-SMA expression level following exposure to TGF-β1 10 ng/mL (22.37 ± 12.86) and highest without TGF-β1 (48.34 ± 13.36), however no morphological changes detected. α-SMA expression level increased with cell passages, decreases with addition of TGF-β1 while not affecting morphology of myofibroblast derived from the orbital socket contracture.
Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Correlation of Malondialdehyde (MDA) and C-reactive Protein (CRP) Level to Neurodevelopmental Outcome in Children After the Episode of Convulsive Type Status Epilepticus Restuningwiyani, Sintha; Ariani, Ariani; Sujuti, Hidayat; Rahayu, Masruroh; Subandiyah, Krisni
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.772 KB) | DOI: 10.11594/jtls.08.03.07

Abstract

Refractory and mortality associated with status epilepticus (SE) were correlated with the degree of inflammation-induced neuronal cell death. This study was aimed to investigate the correlation of oxidative stress (Malondialdehyde, MDA) and inflammation (C-reactive protein, CRP) process with neurodevelopmental outcome in children after the episode of convulsive type SE. This study was designed as cross sectional which included 26 convulsive type SE subjects and 15 control subjects. MDA level was measured by thiobarbituric acid (TBA) method, while CRP level was measured by ELISA method. Neurodevelopmental outcome was measured by Bayley-III Scale of Infant and Toddler Development 3 month after the convulsive type SE episode. Results showed that both MDA (independent t-test, p &lt; 0.05) and CRP (Mann-Whitney test, p &lt; 0.05) level was significantly higher in convulsive type SE group as compared to control group. Further analysis also showed that MDA (Spearman correlation test, p = 0.000, r = 0.756) and CRP (Spearman correlation test, p = 0.000, r = 0.835) level was positively correlated with convulsive type SE. In convulsive type SE group, MDA level was negatively correlated with neurodevelopmental outcome but CRP was not. We concluded that MDA level was negatively correlated with neurodevelopmental outcome in children with convulsive type SE, but CRP was not.
Rho- Kinase Inhibitor Y-27632 Decreases the Thickness of Trabecular Meshwork in Juvenile Rats Mod-el Injected with Sodium Hyaluronate Vierlia, Wino Vrieda; Wulandari, Lely Retno; Sujuti, Hidayat; Effendi, Ma'sum
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.13

Abstract

Most glaucoma drugs lower the intraocular pressure (IOP) by decreasing the aqueous humor production and increasing the outflow through uveoscleral pathway. None of these drugs work mainly on increasing outflow through the trabecular pathway. Consequently, the experiment to develop glaucoma drugs directly target at the trabecular outflow pathway is highly required. The purpose of this study is to reveal the effect of rho-kinase inhibitor Y-27632 on the thickness of trabecular meshwork in juvenile rats model injected with sodium hyalu-ronate.  This study was an experimental study with posttest only control group design. Twenty-four rats were included in this study. Each eye of the rat would be considered as one sample. Samples were divided into 6 groups, negative control group, positive control I group with intracameral sodium hyaluronate injection, posi-tive control II group with topical Y-27632 10 mM, and three experimental groups with intracameral injection of sodium hyaluronate and Y-27632 10-1 mM, 1 mM, and 10 mM respectively. After the procedures all rats were sacrificed and enucleated. Trabecular meshwork tissue was stained with Hematoxilene-Eosin and evalu-ated under 400× microscopic magnification. Quantitative measurements were taken using computerized image analysis with dot slide program.  There were significant statistic differences among the positive control I group and the experimental groups (p-value < 0.05) as well as the positive control II group and the experi-mental groups (p-value < 0.05). The highest mean of decreasing trabecular meshwork thickness was noted in the group given by sodium hyaluronate and Y-27632 10 mM with value of 118.42 µm. There was decreasing thickness of trabecular meshwork due to the effect of rho- kinase inhibitor Y-27632 in juvenile rats injected with sodium hyaluronate.
Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats Yousif, Abdalrauf A Mahmud; Sujuti, Hidayat; Widjajanto, Edi
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.04

Abstract

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.
The effect of Nigella Sativa extract on Alpha-ketoglutarate activity and histopathologic changes on Rat liver induced by Monosodium glutamate Eshami, Ala Shukri; mitaroem, Karyono; Sujuti, Hidayat; Ashour, Abdussalam
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.03

Abstract

         Monosodium glutamate (MSG) is a commonly used food additive and found in most soups, fish and processed meat. The use of MSG in food is growing. Irrational fear had increased in the last few years due to the adverse reactions and toxicity of MSG, which effect on the liver.  Nigella sativa is used as traditional medicine for the treatment of many diseases, it has been extensively investigated in recent years, traditional medicine for the treatment of many disease due to its notable pharmacological properties. NS can inhibit oxidative stress. The present study was undertaken to investigate different Doses of Nigella sativa on alpha KGDH activity and liver histology of MSG induced rat. The animals (n=30) Were grouped  A as (control), B treated with MSG 1g/kg, C MSG treated with NS 0.01g/kg, D MSG treated with NS 0.02g/kg, E MSG treated with NS 0.04g/kg and F treated with NS 0.02g/kg then we investigated the effect alpha KGDH activity by ELISA method and liver histopathology by light microscope.  alpha KGDH activity were significantly increased in treatment MSG compared with treatment negative control, MSG + NS 0.1g/kg, MSG + NS 0.02g/kg, MSG + NS 0.04g/kg, and NS 0.02g/kg. The histological changes in group B and C showed disturbed liver architecture, hemorrhage in the central veins, areas of necrosis, vacuolation and increased inflammatory cells infiltration while group D showed central vein is normal and vacuolation of cytoplasm, group E showed more normal liver architecture as shown less vacuolation in cytoplasm, central vein is normal, and group F showed no pathologic changes.These finding showed that administration of MSG increase alpha KGDH and induced damage in liver tissue. Nigella sativa extract can reduce alpha KGDH and prevent liver damage induced by MSG.Keywords :  Monosodium glutamate, Alpha-ketoglutarate Dehydrogenases, Nigella sativa and liver damage.
Differences in the Levels of Interleukin-1β (IL-1β) and Interleukin-1 Receptor Antagonist (IL-1ra) in Children with Status Epilepticus and Febrile Seizure Shofiyah, Fita; Muid, Masdar; Sujuti, Hidayat
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.05

Abstract

Proinflammatory cytokines are elevated in status epilepticus and febrile seizure and associated with tissue damage. This study aimed to investigate the differences in interleukin 1 beta (IL-1β) and interleukin 1 receptor agonist (IL-1ra) levels in status epilepticus as compared with febrile seizure and febrile. This cross-sectional study was designed to include 45 subjects divided equally into three groups (status epilepticus, febrile seizure, and febrile). Both IL-1β and IL-1ra were measured by using an ELISA method. Results showed that IL-1β levels were significantly higher in the status epilepticus group as compared with the febrile seizure and febrile groups (p < 0.05). IL-1ra levels in the status epilepticus group were significantly lower compared with the febrile seizure group (p = 0.04). Consistently, the IL-1β/IL-1ra ratio in the status epilepticus group was significantly higher as compared with the febrile seizure group (p = 0.01). We concluded that IL-1β and the IL-1β/IL-1ra ratio were significantly higher in status epilepticus. IL-1ra levels were significantly higher in the febrile seizure group.
Agarose Coated Culture Plate in Tumorsphere Culture of Cervical Cancer Cell Line HeLa: an Alternative to Non Adhesive Culture Plate Juniartha, Putu; Indra, Muhammad Rasjad; Sujuti, Hidayat; Lyrawati, Diana; Nurseta, Tatit
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.11

Abstract

Cervical cancer recurs in 90% cases and linked to cancer stem cells that able to self-renew and responsible for recurrence, metastasis, and mortality of cancer. Isolation and identification of cancer stem cells using serum-free medium needs expensive growth factors and consume time. This study try to grow tumor sphere using culture plate coated with 1% agarose as an efficient and economical alternative to non-adhesive culture plate. HeLa cell line was grew in culture plate coated with 1% agarose and non-adhesive culture plate using similar medium and culture condition. Tumor spheres morphology was observed and the colonies were counted in 7 days followed by single cell assay. Tumor spheres then counted for CD133+, CD34+, and Sox2 expression using flowcytometry. Culture plate coated with 1% agarose can be used as an economic and efficient alternative to culture tumor sphere. Using culture plate coated with 1% agarose, the tumor spheres formed in 7 days with similar morphology to non-adhesive culture plate. Tumorsphere had three dimensional – sphere shape that tightly attached, colonized, and overlapped. The tumor sphere colony counts of two plates were statistically have no significant difference (p=0,667). Single cell assay of a tumor sphere shows that it can grow new tumor spheres with similar morphology. The tumor sphere from culture plate coated with 1% agarose express CD133+ and CD34+ as much as 8.78% ± 2.14 and Sox2 as much as 35.30% ± 23.82 whereas tumor sphere from non-adhesive culture plate express CD133+ and CD34+ as much as 62.36% ± 1.06 and Sox2 as much as 98.86% ± 0.56 (p = 0000).
Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats Abdalrauf A Mahmud Yousif; Hidayat Sujuti; Edi Widjajanto
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.04

Abstract

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.
Co-Authors A. Harijono A.A. Ketut Agung Cahyawan W Abdalrauf A Mahmud Yousif Abdalrauf A Mahmud Yousif, Abdalrauf A Mahmud Abdussalam Ashour, Abdussalam Achmad Rudijanto Adinda Chika Anindita Affandi, Haykal Agung Prasetyo Wibowo Agustiana Dwi Agustiana Dwi Indiah Ventiyaningsih Agustina Tri Endharti Ala Shukri Eshami, Ala Shukri Analis W Wardhana Anik Puryatni Anisak, Siti Ariani Ariani Ariani Ariani Arifin, Mochammad Samsul Arsinah Habibah Fitriah, Arsinah Habibah Askandar Tjokroprawiro Astika Swastirani Aulanni'am, Aulanni'am Aulia Yasmin, Aulia Bagus Hermansyah Bambang Rahardjo Bayu Kurniawan Bintang Soetjahjo Bogi Pratomo Wibowo Burhan, Niniek Chabibi, Mochamad Chabibi Chairinnisa, Elsa Safira Christina Dewi Dalhar, Mochamad Dewi, Ni Ketut Ayu Feriyanti Dian Handayani Dian Nugrahenny Diana Lyrawati Diana Lyrawati Dicky Faizal Irnandi Didi Candradikusuma Didi Candradikusuma Dwi Jayasa, Pande Made Dwi Yuni Nur Hidayati Edi Mustamsir Edi Widjajanto Edi Widjajanto Edwin Widodo Effendi, Ma'sum Eko Sulistijono Elok Zubaidah Erma Sulistyaningsih Ery Olivianto Fardizia Putri Alia Feni Istikharoh Fibrianto, Yuda Fita Shofiyah Gading, Inges Manggar Gerry Gunawan, Gerry Handono Kalim Hanif Hanif Happy Kurnia Permatasari Hara, Kenta HARI PURNOMO Hariwati Hariwati Hasfiani, Yuliatin Huda Rohmawati Husna, Ully Husnul Khotimah Ika Kustiyah Oktaviyanti Indrayanti Indrayanti Indriati Dwi Rahayu Inggita Kusumastuty Josef Sem Berth Tuda Juniartha, Putu Kalsumy, Umi Kanthi Permaningtyas Tritisari, Kanthi Permaningtyas Karyono Mintaroem Karyono Mintaroem Karyono Mintaroem Karyono mitaroem, Karyono Krisni Subandiyah Krisni Subandiyah Kusworini Handono Laily Yuliatun Lavina Sofia Ardani Lely Retno W Leny Budhi Harti Lestari, Bayu Lirawati, Diana Loeki Enggar Fitri M Rasjad Indra Machlusil Husna, Machlusil Maimun Z Arthamin, Maimun Z Masafumi Matsuo Masdar Muid Masdar Muid Masruroh Rahayu Masruroh Rahayu, Masruroh Mayangsari, Elly Mentari Sekar Arum Miftahu Soleh Moch. Aris Widodo, Moch. Aris Mochammad Dalhar Mohammad Hidayat Mohammad Hidayat Mohammad S. Rohman Muhammad Ali Faisal Muhammad Rasjad Indra Muhammad Rasjad Indra Nabila Andjani, Nabila Nabila Erina Erwan Nashi Widodo Nathania Bella Claresta Niarti Ulan Sari Siarnu Nindy Sabrina Niniek Burhan Nisrina Nur Afina, Nisrina Nur Nunuk Helilusiatiningsih Nur Kusmiyati Nur Permatasari Nura Fattah Cantika Yoga Nurshalilah Nurshalilah Pande Made Dwi Jayasa Pande Made Dwijayasa Prima Wijayanti, Prima Primawardani, Putri Puryatni, Anik - Putri, Dianita Setya Pradita Putu Juniartha Raisa, Neila Raudhatul Jannah Restuningwiyani, Sintha Retnani, Diah Prabawati Retty Ratnawati Retty Ratnawati Rizky Amalia RR. Ettie Rukmigarsari Salim, Irfan Agus Samsul Arifin Santoso, Giovani Anggasta Satrijo, Budi Selvi Marcellia Septi Nur Rachmawati Setiyaningsih, Fera Yuli Setyawati Soeharto Setyawati Soeharto, Setyawati Shintiya Dewi, Debby Shofiyah, Fita SIMON BAMBANG WIDJANARKO Sintha Restuningwiyani Siti Lintang Kawuryan Siti Narsito Wulan Soetjahjo, Bintang Sri Andarini Sutrisno Sutrisno Syahrul Chilmi, Syahrul Syarifah Rohaya Tatit Nurseta Tatit Nurseta Tatit Nurseta Tika Ardhini Wardoyo Tinny Endang Tinny Endang Hernowati Tokunaga, Chiharu Tri Dewanti Widyaningsih Triawanti Triawanti Tuda, Josef Sem Berth Umar Zein Vierlia, Wino Vrieda Widowati, Hesty Widya Rahmawati Winda Nurtika Yonezawa, Kazuyoshi Yoshino, Ken-ichi Yuda Fibrianto Yudanto, Hendy Setyo Zuly Vita Aulya, Zuly Vita