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Potensi Aktivitas Antioksidan Pada Tanaman Obat Yang Dihasilkan Oleh Mikroba Endofit Sebagai Potensi Rekayasa Genetika Molekuler Melalui Gen Editing (Kajian Pustaka) Tri Pamungkas, Barolym; Rezaldi, Firman; Suminar, Erni; Putri Ginaris, Rifkarosita; Trisnawati, Desi
JAGO TOLIS : Jurnal Agrokompleks Tolis Vol 5 No 2 (2025): Mei
Publisher : Universitas Madako Tolitoli

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56630/jago.v5i2.754

Abstract

Pencarian senyawa metabolit sekunder yang berhasiat sebagai sumber antioksidan jangka panjang dapat dihasilkan melalui metode bioteknologi yang cukup sederhana hingga modern baik dengan menggunakan kemampuan mikroba endofit sampai penggunaan teknik rekayasa genetika molekuler yang saat ini cukup populer yaitu gen editing. Jaringan tanaman yang berpotensi dalam menghasilkan mikroba endofit meliputi bunga, buah, batang, daun, akar, dan biji yang berperan penting dalam memproteksi tanaman inang dari cekaman lingkungan dan mikroorganisme pesaing. Salah satu jenis jamur mikro yaitu Muscodor albus telah terbukti mampu memproduksi campuran senyawa organik volatil dari tanaman kayu manis. Senyawa organik volatil tersebut memiliki aktivitas farmakologi sebagai sumber antimikroba dalam spektrum luas. Taxomyces andreane merupakan salah satu kapang endofit yang diproduksi dari tanaman Paclitaxel dan berpotensi dalam menghasilkan senyawa taksol yang memiliki aktivitas farmakologi sebagai sumber antikanker. Pestalotiopsis microspore merupakan salah satu kultur mikroba penghasil senyawa pestacin dan isopestacin dari tanaman Ketapang dan berfungsi sebagai sumber antioksidan maupun antikanker. Antioksidan digolongkan menjadi antioksidan endogen antioksidan enzim dan vitamin. Rekayasa genetika dengan memanfaatkan Agrobacterium tumafaciens berpotensi dalam menghasilkan sifat yang dibutuhkan atau diinginkan sesuai target melalui penyisipan gen dari organisme yang berperan sebagai pendonor gen menuju organisme penerima gen. Gen lancolate merupakan salah satu gen yang berhasil disisipkan untuk mengkode daun tomat dan merubah daun majemuk dalam kondisi besar menjadi lebih kecil. Metode gen editing yang direkomendasikan berupa CRISPR-Cas 9 karena dapat menyunting gen lancolate pada buah tomat dengan urutan genom diploid yang jelas.
Metode Bioteknologi Tanaman Lavender (Lavandula Officinalis) Jangka Panjang Sebagai Aromaterapi Bagi Penyakit Genetik Yang Terekspresi Gen Target Level Molekular (Kajian Pustaka). Rezaldi, Firman; Erni Suminar; Syariful Mubarok; Vevi Maritha; Roni Gumilar; Elok Zubaidah
Jurnal Ilmiah Kesehatan Delima Vol. 7 No. 2 (2025): Jurnal Ilmiah Kesehatan Delima
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Sekolah Tinggi Ilmu Kesehatan Salsabila Serang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60010/jikd.v7i2.128

Abstract

Lavender is a plant that has various potentials to be developed in various therapeutic aspects, especially as an immunomodulator. Lavender oil is an active ingredient that has quite a lot of potential to be developed as the latest therapeutic agent where its use can be used by inhalation, aromatherapy massage, aromatherapeutic baths, and saunas. The results of metabolomic analysis from previous research results lavender contains alpha-pinene (0.44%), camphene (0.30%), beta-pinene (0.41%), beta mycene (1.11%), acetic acid (0. .74%), limonene (1.50%), cineole (4.72), linalool (36.55%), camphor (3.16%), terpinine-4-ol (2.07%), linalyl acetate (32.54), geranyl acetate (1.79%), nerly acetate (1.14%), and caryophyllene (7.55%). Thyme can decrease the production and gene expression of pro-inflammatory mediators, including Tumor Necrosis Factor-? (TNF-?), interleukin-(IL) 1B, and IL-6, and increase the marker for the anti-inflammatory cytokine IL-10. Chamomile is also used for the treatment of inflammatory diseases. Borage has gamma linoleic acid, which suppresses TNF-?. It increases prostaglandin-E levels leading to augmentation of cyclic adenosine monophosphate. Efforts need to be made to develop lavender as a herbal therapeutic in the context of modern plant biotechnology through a primordial stage (callus) tissue culture approach, biotic elicitors, and Cas-9.
Penguatan Konsep Berbasis Pertanian Berkelanjutan di Negara India sebagai Solusi Mengurangi Efek Gas Rumah Kaca (Literature Review) Yudianto, Tri; Pramono, Edi; Rezaldi, Firman; Rahmad, Sukardi Sugeng; Yenny, Ratna Fitry; Gumilar, Roni; Trisnawati, Desi; Ginaris, Rifkarosita Putri; Suminar, Erni
Jurnal Agroteknologi (Agronu) Vol 4 No 01 (2025): Jurnal Agroteknologi (Agronu)
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53863/agronu.v4i01.1521

Abstract

The conventional agricultural system that is applied tends to use chemicals such as pesticides, on the one hand, providing various positive impacts, including increasing productivity quickly, meeting food needs efficiently, and helping food insecurity as often occurs in India. Sustainable agricultural systems are aimed at reducing environmental damage, maintaining agricultural productivity, increasing farmers' income and improving the stability and quality of life of rural communities. Three big indicators that can be seen are that the environment is sustainable, the economy is improving (prosperous), and it is socially accepted by the farming community. Other negative impacts on conventional agricultural systems include decreasing soil quality, giving rise to new pests and diseases, environmentally friendly microorganisms becoming extinct, toxic chemicals threatening the condition of the biosphere in the food chain. The concept of organic farming that is applied ideally is based on optimal, balanced, efficient management of land, water and land, so that existing biodiversity is one of the external efforts to provide a positive impact. In writing this literature review, the sections that will be discussed include the application of conventional agricultural systems that occur in India, suggestions and improvements that can be implemented based on potential such as modeling greenhouse gas emissions from agriculture, GHG (Greenhouse Gas) mitigation from agriculture, absorption of element C in agricultural soil, conservation agriculture, reducing nitrous oxide emissions, economic implications of greenhouse gas mitigation, and the use of butterfly pea flower kombucha in agricultural aspects
Pelatihan Kultur Jaringan bagi Tim Binaraja Millennials Smart Farming (BMST) Untuk Perbanyakan Bibit Pisang Roid Berkualitas: Tissue Culture Training for the Binaraja Millennials Smart Farming (BMSF) Team to Produce High-Quality Roid Banana Seedlings Suminar, Erni; Anne Nuraini; Murgayanti; Farida Damayanti; Citra Bakti; Santika Sari; Ade Ismail; Syariful Mubarok; Meisyela Erizon; Fathi Rufaidah; Eva Aprilia
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 1 (2025): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pisang roid lokal merupakan tanaman yang banyak dikembangkan produk turunannya oleh masyarakat di daerah kecamatan Jatigede. Terdapat kendala dalam perbanyakan tanaman pisang roid lokal yang dilakukan secara konvensional seperti ketersediaan bibit berkualitas unggul. Dengan demikian, diperlukan alternatif perbanyakan bibit unggul yang dapat dilakukan dengan perbanyakan kultur jaringan. Perbanyakan kultur jaringan memungkinkan perbanyakan bibit pisang dengan pertumbuhan cepat dengan seragam dan tahan terhadap hama dan penyakit. Akan tetapi, saat ini wawasan tentang teknik kultur jaringan yang baik masih terbatas. Oleh karena itu, diperlukan suatu kegiatan pendampingan dan pelatihan agar perbanyakan pisang dapat dilakukan melalui kultur jaringan. Kerjasama dengan kelompok pemuda di Jatigede yaitu Binaraja Millennials Smart Farming (BMSF) juga diperlukan mengingat terdapat visi yang sama untuk memberdayakan generasi muda, regenerasi petani serta menciptakan pertanian yang berkelanjutan. Kolaborasi perlu menekankan sinergi dari komunitas, pemerintah, industri dan bisnis untuk memastikan tercapainya tujuan bersama yang berkelanjutan. Tujuan kegiatan pengabdian ini di antaranya: (1) memiliki mitra yang paham mengenai kultur jaringan pisang baik secara teoritis maupun praktis, (2) tersedianya koleksi plasma nutfah tanaman pisang roid in vitro sebagai tanaman indukan, dan (3) diperolehnya metode kultur jaringan pisang roid yang efektif dan efisien.
PENGARUH POTENSIAL OSMOTIK LARUTAN EKSTRAK BAWANG MERAH TERHADAP OPTIMALISASI MUTU FISIOLOGIS BENIH KEDELAI (Glycine max L.) KULTIVAR ANJASMORO YANG TERDETERIORASI Kadapi, Muhamad; Suminar, Erni; Na’im, Aidi
JURNAL AGROTROPIKA Vol 24, No 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9848

Abstract

The longevity of soybean seed was caused to chemical compound such the protein that being easy to change by in environmental conditions during storage. Therefore, the application in increasing vigor of seed before sowing is required such as organic priming. This study aimed to determine the effect of osmotic potential treatment of shallot extract solution on the physiological quality of deteriorated soybean seeds, Anjasmoro cv based on the different osmotic potential. The research was conducted from August to September 2022 at the Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University. The experimental design used in this study was a completely randomized design (CRD) with four treatment levels of osmotic potential: 0.04 MPa (control), -0.06 MPa, -0.08 MPa, and -0.27 MPa, and four replications and duplo for each treatment. The data results were analysed using ANOVA at the 5% significance level and the post hoc analysis used the Least Significant Difference test at the 5% level of significance. The results showed significant differences in the germination rate, vigour index, growth speed, and simultaneity of growth. In contrast, there was a non-significant in the normal dry weight of seedlings among treatments. The osmotic potential treatment of shallot extract solution at -0.08 MPa affects physiological quality as shown in germination rate (75.94%), vigor index (5.87), growth speed (30.78%/ethmal), and growth simultaneity of growth (63.75%) trait. This study suggests that the osmotic potential content of shallot extract solution can optimize the physiological quality of deteriorated soybean seeds.
A review on detection of drought stress in tea plants through morphological, physiological, and biochemical approaches Anjarsari, Intan Ratna Dewi; Suminar, Erni; Wiharti, Nabila Ragil
Kultivasi Vol 24, No 1 (2025): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v24i1.62127

Abstract

Tea plants (Camellia sinensis) are important commodities with high economic value, but their production is greatly affected by environmental stresses such as drought and extreme temperatures. Global warming in recent years has led to extreme weather events and an increase in the earth's temperature, which also causes drought. Drought stress is one of the most significant abiotic factors affecting crop productivity. However, despite significant progress, there remains a notable gap in research, particularly the lack of integrated studies that combine morphological, physiological, and biochemical indicators for early and precise detection of drought stress in tea plants. To identify and mitigate the impact of this stress, a comprehensive approach is needed that includes morphological, physiological, and biochemical aspects. The morphological approach includes changes in leaf structure, stomatal size and number, and root growth patterns. From a physiological perspective, plant response to drought can be seen through measurements of transpiration rate, leaf water potential, and photosynthetic capacity. On the biochemical side, the accumulation of compounds such as proline, antioxidant enzymes, and stress-related hormones, such as abscisic acid, plays a crucial role in plant adaptation to drought conditions. This review summarizes the latest findings related to these indicators by analyzing relevant articles published between 2007 and 2024, obtained through reference searches on Google Scholar and academic databases such as Scopus, EBSCO, and Clarivate. The articles were further analyzed using descriptive methods. This review aims to summarize the latest findings related to these indicators and identify the most effective methods for detecting drought stress in tea plants. A deeper understanding of the tea plant's response to drought through these three approaches is expected to provide a solid basis for developing better drought management strategies to maintain tea plant productivity amid increasingly extreme climate change.
Effect of meta-topolin and kinetin at various concentrations on shoot multiplication in white turmeric (Curcuma zedoaria Rosc.) Murgayanti, Murgayanti; Azizah, Annisa Nanda Nur; Nuraini, Anne; Suminar, Erni; Mubarok, Syariful
Kultivasi Vol 24, No 1 (2025): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v24i1.61664

Abstract

White turmeric is a medicinal plant widely used to treat various diseases. The limited availability of white turmeric seedlings is influenced by the scarcity of quality seeds due to the long dormancy of the rhizomes and the high incidence of pathogen attacks. Tissue culture techniques are one alternative to address the problem of white turmeric seedling availability. This study aims to determine the best concentrations of meta-topolin and kinetin for in vitro shoot multiplication of white turmeric. The research was conducted from January to June 2022 at the tissue culture laboratory, Faculty of Agriculture, Universitas Padjadjaran. This study used a completely randomized design consisting of 7 treatments: control (0 mg L-1), meta-topolin (1 mg L-1; 2 mg L-1; 3 mg L-1), kinetin (1 mg L-1; 2 mg L-1; 3 mg L-1). Analysis of variance (ANOVA) and Duncan’s multiple range test were used to analyze the statistical effects of meta-topolin and kinetin application. Applying 1 mg L-1 meta-topolin yielded the best results for shoot number, indicating that 1 mg L-1 meta-topolin is a potential cytokinin for the propagation of Curcuma zedoaria Rosc. 
PENGARUH POTENSIAL OSMOTIK LARUTAN EKSTRAK BAWANG MERAH TERHADAP OPTIMALISASI MUTU FISIOLOGIS BENIH KEDELAI (Glycine max L.) KULTIVAR ANJASMORO YANG TERDETERIORASI Kadapi, Muhamad; Suminar, Erni; Na’im, Aidi
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9848

Abstract

The longevity of soybean seed was caused to chemical compound such the protein that being easy to change by in environmental conditions during storage. Therefore, the application in increasing vigor of seed before sowing is required such as organic priming. This study aimed to determine the effect of osmotic potential treatment of shallot extract solution on the physiological quality of deteriorated soybean seeds, Anjasmoro cv based on the different osmotic potential. The research was conducted from August to September 2022 at the Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University. The experimental design used in this study was a completely randomized design (CRD) with four treatment levels of osmotic potential: 0.04 MPa (control), -0.06 MPa, -0.08 MPa, and -0.27 MPa, and four replications and duplo for each treatment. The data results were analysed using ANOVA at the 5% significance level and the post hoc analysis used the Least Significant Difference test at the 5% level of significance. The results showed significant differences in the germination rate, vigour index, growth speed, and simultaneity of growth. In contrast, there was a non-significant in the normal dry weight of seedlings among treatments. The osmotic potential treatment of shallot extract solution at -0.08 MPa affects physiological quality as shown in germination rate (75.94%), vigor index (5.87), growth speed (30.78%/ethmal), and growth simultaneity of growth (63.75%) trait. This study suggests that the osmotic potential content of shallot extract solution can optimize the physiological quality of deteriorated soybean seeds.
Improvement of Soil Chemical Properties of Typic Hapludult After Application of Organic and Inorganic Fertilizers Mubarok, Syariful; Kusumiyati, Kusumiyati; Muhardiono, Iman; Yuniarti, Anni; Rosniawati, Santi; Suminar, Erni
JOURNAL OF TROPICAL SOILS Vol. 22 No. 3: September 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i3.131-137

Abstract

Tuberose (Polyanthes tuberosa L.) is a potential flowering plant that will be developed in Jatinangor, West Java, Indonesia. However, the characteristics of Typic Hapludult soil in Jatinangor , i.e. clay texture, acidic pH, and high amount of total-P but low in available-P cause the tuberose difficult to grow optimally. The aim of this study was to obtain the potential ratio of organic and inorganic fertilizer dosages for tuberose grown on Typic Hapludult. The combinations of organic and inorganic fertilizers with different ratios (0/0; 0/1; 1/0; 0.5/0.5; 0.5/1; 1/0.5; 1/1.5; 1.5/0; 1.5/0.5, and 1.5/) were applied. The application of the combination of organic and inorganic fertilizers showed positive effects on soil pH, available-P, total-P, and fresh weight of tuberose grown on Typic Hapludult. The application of 50% organic fertilizer + 50% inorganic fertilizer was an effective combination that was able to increase the fresh weight of tuberose up to 9240 g plant-1 or increasing the fresh weight by 39% in comparison to that in the control treatment (without fertilizer application). 
PHENYLALANINE-INDUCED MODULATION OF CALLUS CHARACTERISTICS AND SECONDARY METABOLITE ACCUMULATION IN Ocimum basilicum L. UNDER IN VITRO CONDITIONS Suminar, Erni; Mubarok, Syariful; Budiarto, Rahmat; Yulianto, Fiky; Nuraini, Anne; Yuniarti, Anni; Kusumadewi, Vira; Meliansyah, Rika; Kurnia, Dikdik; Julaeha, Siti
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.11044

Abstract

Basil (Ocimum basilicum L.) is widely used in traditional medicine due to its rich content of phenolic and flavonoid compounds. However, the natural production of these metabolites is limited. Callus culture offers a controlled method to enhance their accumulation, with medium composition, particularly precursor supplementation, playing a critical role. Phenylalanine, an aromatic amino acid, is a key precursor in the biosynthesis of phenolics and flavonoids via the shikimate pathway. This study aimed to evaluate the effect of phenylalanine on callus growth and the accumulation of total phenolic and flavonoid compounds in basil. The experiment was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, using Murashige and Skoog (MS) medium with phenylalanine concentrations of 0, 1.3, 1.6, and 2 g.L-1, each replicated six times. Results showed that phenylalanine treatments caused brown coloration, compact callus texture, and inhibited growth, as indicated by reduced fresh and dry weights. The 1.6 g.L-1 treatment produced the highest total phenolic content, while the 2 g.L-1 treatment yielded the highest flavonoid content. These findings suggest that phenylalanine can enhance secondary metabolite accumulation in basil callus, although it may suppress biomass growth.
Co-Authors A.A. Ketut Agung Cahyawan W Ade Ismail Ade Ismail Ade Ismail Ade Ismail Ade Setiawan Agung Rahmadi Agung Rahmadi Agus Purwito Alin Robiah Al Adawiyah Alvianto, Muhamad Amalia, Inneke ANNE NURAINI Anne Nurani ANNE NURBAITY Anni Yuniarti Arin Rosmala Ariyanto, Nur Budi Ayu Ratna Ningrum Ayuwardani, Novi Azizah, Annisa Nanda Nur Bambang Nurhadi Bella Dian Ratnasari. Bella Dian Ratnasari., Bella Dian Camelia Andriani Citra Bakti, Citra Denny Sobardini Denny Sobardini Denny Sobardini Denny Sobardini Sobarna Denny Sobardini Sobarna Denny Sobardini, Denny Dian Ardiansyah Dian Ardiansyah Dikdik Kurnia Dilla Nitra Gustiana Dinnur Afiifah Dinnur Afiifah, Dinnur Dirga Sapta Sara Dwi Hastuti Edi Pramono Ega Raisya Ega Raisya Elma, Tiara Elok Zubaidah Endah Yulia Erizon, Meisyela Salsabila Eva Aprilia Fadhillah, Maya Farida Damayanti Farida Farida Fauzia Khaerunnisa Millenia Fitri Widya Fitriatin Fitri Widya Lestari Fitrianti Widya Lestari G. G. Pitaloka G. G. Pitaloka, G. G. Ganjar Herdiansyah Gina Gustiani Pitaloka Gumilar, Roni H. Apriyanto H. Apriyanto, H. Hapizhah Hapizhah Hapizhah Hapizhah, Hapizhah Hiroshi Ezura Hiroshi Ezura Inneke Amalia Intan Ratna Dewi Anjarsari Iwan Setiawan Jajang Sauman Hamdani Kusumadewi, Vira Kusumiyati Lamro Purba Lindung Tri Puspasari Marhan Nurullia Maritha, Vevi Masako Akutsu Meddy Rachmadi Meisyela Erizon Mia Munggarani Mira Ariyanti Mita Indriani Muhamad Alvianto Muhamad Kadapi Muhamad kadapi, Muhamad Muhammad Abdillah Hasan Qonit Muhardiono, Iman Murgayanti Murgayanti Mustika Arsri Na’im, Aidi Nita Suswati Endah Rini Noladhi Wicaksana Noor Istifadah Nur Azizah Romadhoni Nur Budi Ariyanto Nursuhud Nursuhud Pamungkas, Barolym Tri Pujawati Suryatmana Rahmad, Sukardi Sugeng Rahmat Budiarto Rahmawati, Vira Raisya, Ega Rani Andriani Budi Kusumo Reginawanti Hindersah Rezaldi, Firman Rifkarosita Putri Ginaris Rija Sudirja RIKA MELIANSYAH Roni Gumilar Rosniawati, Santi Rubaekah, Siti Sarah Rufaidah, Fathi S Sumadi Santi Rosniawaty Santika Sari SIska Rasiska, SIska Siti Julaeha, Siti Siti Rakhmah Tenrisui Pakki Siti Rakhmah Tenrisui Pakki Siti Sarah Rubaekah Sobir Sobir Sri Hartati Suci Azima, Nuzula Sudarjat Sudarjat Sudarjat Sulistyaningsih Sulistyaningsih Sulistyaningsih Sulistyaningsih Sumadi Sumadi SUMADI SUMADI Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi SYARIFUL MUBAROK Tiara Elma Toto Sunarto Trisnawati, Desi Venny Revia Viola Vevi Maritha Vijaya Isnaniawardhani Wawan Sutari Wieny Heriliya Rizky Wieny Heriliya Rizky, Wieny Heriliya Wieny Marma Jaya Wieny Rizky Wiharti, Nabila Ragil Y. Sholihin Y. Sholihin, Y. Yayat Rochayat Suradinata Yenny, Ratna Fitry Yudianto, Tri Yudithia Maxiselly, Yudithia Yulianto, Fiky