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Taryono, Taryono
Departemen Budidaya Pertanian, Fakultas Pertanian, Universitas Gadjah Mada
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Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Munawarti, Aminatun; Taryono, Taryono; Semiarti, Endang; Sismindari, Sismindari
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Aminatun Munawarti; Taryono Taryono; Endang Semiarti; Sismindari Sismindari
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Co-Authors . Suyadi . Suyadi Abda Abda Adia Pratama Dewi Afriana, Wendra Afriza, Yunika Nuuru Agus Anggoro Sigit Aguslan Aguslan Ahmad Muhtadi Ahmad Muhtadi Rangkuti Ajeng Retno Setiawati Alfaiz, Ghufron Alfi Ma'rifah Aliyin, Sri Nur Amelia Agustina Putri Aminatun Munawarti Aminatun Munawarti, Aminatun Ana Melani Andreas Setiawan Andrew Budiherlando Annisa, Permata Humaira Anthoni Mayes Anto Rimbawanto Anto Rimbawanto Anto Rimbawanto Anton Rimbawanto Ari Murti Ahmadi Arif Munandar Arif Satria Aris Eddy Sarwana Aryati, Ida Atrio Andika Aulia, Nurfitri Awibbi Permana Meidil Putra AYPBC Widyatmoko AYPBC Widyatmoko Ayub, Made R.S.S.N Booth, Hollie Budhi H. Iskandar Charles Parningotan Haratua Simanjuntak Cicilia Widiarini P.U. Danu Santoso Darmawan, Imam Defaro, Dafa Deny Setiawan Despiandra, Aulia Desweni, Selly Prima Deva Deva Dewi Ratna Nurhayati Dewi Ratna Nurhayati Dewi Ratna Nurhayati Dhemas Adi Purwa Dhian Herdhiansyah Didik Indradewa Didik Purwadi Didin S. Damanhuri Dinda Tria Handayani Djoko Prajitno Dody Kastono dwi aini Dwi Hartati Efendi, Suryono Eko Hanudin Eko Hanudin Ekwarso Hendro Elia, Yemima Elisa Anggraeni Endang Semiarti Endang Sulistyaningsih Epraim Theopilus Sitepu Eriyati, Eriyati Erlina Ambarwati Ernayunita Ernayunita Etty Riani Fahmi Zuriyadi Fathin Nabihaty Fatwa, Esa Buana Fauzi, Rahmat Dwi Fery Kurniawan FERY KURNIAWAN Firmansyah Firmansyah Firmansyah Firmansyah Fitrah Annisa Fredinan Yulianda Hafidh Izzudin Hafiza Fikri Naipospos Handoko Handoko Hasanudin Hasanudin Haviah ilmiah Hedianto, Dimas Angga Hefni Effendi Hendro Ekwarso Hendro, Ekwarso Henricus Tegar Panuntun Hermana, Indra Setiadi Hermansyah, Rizky Fajar Hermanto, Koko Hernawan Y Rahmadi Hidayat Hidayati, Nafisah Arina Hidayati, Sri Wahyu Hutasoit, Anastasya I Gusti Agung Komang Diafari Djuni Hartawan Ilmiah, Haviah Hafidhotul Imam Hidayat Imam Nur Huda Iman, Topan Rahmatul Iman Indra, Sukiadi Irmayani, Festi Irmayani, Festi ismayuni, Windia Istiana Prihatini Isyandi Isyandi Junaedi Abdillah K. Kristamtini Kadarwan Soewardi Kadarwan Soewradi Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini, Kristamtini Kristamtini, Kristamtini Kurniasih, Budiastuti Kurniasih, Cut Endang Kuswandono, Agung Lapeti Sari Latiful Muttaqin Lilik Sutiarso Lisamy, Sisilia Eka Aisyah Lukita Uli Hotma Luky Adrianto M. Mukhlis Kamal, M. Mukhlis M. Zulfan Arrodli M.Gunawan Machfud Machfud Manurung, Afriandi Mardika R, Richa Marietadewi, Astrid Marimin , Marimin Marimin Maryam Muharroron Mayes Anthony Mei Edi Prayitno Milla, Milla Achliza Putri Misdawita, Misdawita Moch. Agus Choiron Mohammad Mukhlis Kamal Mohammad Yanuar Setya Wibowo Muhamad Nurman Muhammad Arsyad Syahputra Muhammad Habib Widyawan Muhammad Ichsan Muhammad Irfan Sholehudin Muhammad Rifa’I Muhartini Muhartini Mukarromah, Aminah Mursito, Bambang Murti, Rudi Hari Mustaqim, Imam Musyaffa, Dzikri Amali Nababan Warni Lincu Nandita Oktaviana Nasrullah Nasrullah Nasrullah Nasrullah Nasrullah Nasrullah Nasrullah Nasrullah Nasrullah, Nasrullah Nolianus Hogejau Nolianush Hogejau Nurfaiqah, Seplina Nurfiarini, Amula Nurseptiani, Sifa Nurul Irdina Pangestuti, Triana Panjisakti Basunanda Paramita Cahyaningrum Permatacita, Fitriah Prapto Yudono Prapto Yudono Prapto Yudono Priska Dian Cristina Pusparini, Intan Putri, Fevi Rahma Dwi Putri, Vivian Putro Sukirno Tri Raditya, Masayu Adara Rafita, Fadil Rahmad, Ali Nur Ramadani, Oktavia Nada Rani Agustina Wulandari Rani Agustina Wulandari Ratna Dewi Eskundari Rendy Alfaid Retno Sunarminingsih Sudibyo Ridho, Muhammad Habib Rifka Safira Rilus Kinseng Rita Yani Iyan Ritayani Iyan Rizki Diah Afritanti Rodiansyah, Yubel Roza Auliya Rosana MZ Rudi H. Murti Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Sam'un, Mohamad Sarkasih, Muhammad Reza Sasmita, Lidya Septiani Rahayu Sholeh Udin Al Ghifari Siahaan, Mutiara Lasnita Sigid Hariyadi sigit hariyadi, sigit Silaban, Rouli Margareta Siregar, Bonza Gurti Zahza Siska Permata Siska Permata Siska Permata, Siska Sismindari . Sismindari Sismindari Sri Adi Kadarsih Sri Adikadarsih Sri Adikadarsih, Sri SRI RAHAYU Sri Ratna Ningsih Ginting Sri Wening Sugiharto Budi Santoso Sugiharto Budi Santoso, Sugiharto Budi Sukiadi Indra Supriyanta Supriyanta Surjanto, Didiek Suyadi Mitrowihardjo Suyadi Suyadi Syapsan, Syapsan Tanika D. Sofianti Taufan Alam Taufiq Ahmad Romdoni Tohari Tohari Triani, Imelda Ufira Isbah Usman, Agus Utama, Darma Vania Mugareta, Atmaja Vanska Nozelle Hermanto Vina Eka Aristya Vina Eka Aristya Vina Eka Aristya Vina Eka Aristya Vina Eka Aristya Wahyu Hamidi Wawan Sulistyono Wibowo, Raden Bagus Tri Joko Widiastuti, Paramita Widiastuti, Paramita Wulandari, Rani Agustina Y. Prapto, Y. Yandi Jefri Yasinta Pinilih Yekti Asih Purwestri Yelly Zamaya Yessy Maharani Yudy Surya Irawan Yuli Priyana Yuni Rahmawati Simangunsong Yurna Yenni Yusiana, Ekalia Yuwono, Nasih Widya Zainalarifin, Jauhar Zairion Zulfikar, Andi Zulfita, Nasywa Zuriyadi, Fahmi