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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Biotechnology Biota Mutiara Medika: Jurnal Kedokteran dan Kesehatan Pharmacon Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Biologi Tropis Jurnal Medik Veteriner Proceedings of the International Conference on Applied Science and Health Global Health Management Journal Biota Jurnal Biomedika JURNAL PERIKANAN TROPIS Narra J Makara Journal of Science Biological Environment and Pollution Rumphius Pattimura Biological Journal Berkala Ilmiah Biologi Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Nastiti Wijayanti
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Exploring the Antioxidant and Anti-proliferative Effects of Murraya koenigii (L.) Leaves Methanol Extract on T47D Breast Cancer Cell Lines Nisa, Mutia Khoirun; Salamah, Rohmi; Wijayanti, Nastiti
GHMJ (Global Health Management Journal) Vol. 8 No. 1 (2025)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-811204

Abstract

Background: Murraya koenigii (Curry) leaves are herbal plants that have bioactive compounds such as phenolics, flavonoids, and alkaloids that function as antioxidants and anti-cancers. Aims: This study aimed to quantitatively determine the bioactive compounds in curry leaves by calculating phenolic, flavonoid, and alkaloid content. Its potential as an antioxidant and anti-proliferative compound in T47D breast cancer cell lines was also analyzed. Methods: Murraya koenigii leaves extract was obtained by maceration using 80% methanol (1:5 w/v), then the antioxidant and anti-proliferative test was carried out using the 1,1-Diphenyl-2-Pycrylhidracyl (DPPH) and MTT (-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay, respectively, followed by flow cytometry to determine the apoptotic activity of the extract on T47D breast cancer cell lines. Results: The results for the bioactive compounds in the methanol extract of Murraya koenigii leaves were 156.62±1.49 mg/g phenolics, 99.19±0.25 mg/g flavonoids, and 2.90±0.01 mg/g alkaloids. The IC50 value for antioxidant activity was 25.058±2.2 μg/ml and showed an anti-proliferative effect on T47D cells in a dose-dependent manner with IC50 74.71±5.45 μg/ml for cytotoxicity. Furthermore, flow cytometry showed that a concentration of 1/16 IC50 has the best results for apoptosis. Conclusion: The methanol extract of Murraya koenigii leaves has the potential as an antioxidant and anti-proliferative agent and can induce apoptosis of T47D cells.
Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection Widiastuti, Dyah; Paramita, Dewi Kartikawati; Murwanti, Retno; Kusrini, Ina; Wijayanti, Nastiti; Haryanto, Aris
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111102

Abstract

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.
Codon Optimization and Epitope Identification of L1 Protein Human Papillomavirus Type 52 for Expression in Lactococcus lactis: Codon Optimization and Epitope Identification L1 Protein HPV52 Prastyowati, Anika; Hakim, Mohamad Saifudin; Wijayanti, Nastiti; Wibawa, Tri
Journal of Tropical Life Science Vol. 15 No. 3
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.03.03

Abstract

Human papillomavirus (HPV) type 52 is among the top three high-risk oncogenic types that are associated with cervical cancer worldwide, especially in Indonesia. Designing a vaccine against HPV using an Lactococcus lactis expression system is a promising strategy, as this bacterium is well-known for its probiotic properties and can be safely utilized as a delivery carrier for oral vaccines. This study aimed to optimize the codon usage of the L1 protein of HPV type 52 for expression in L. lactis expression system and to identify potential epitopes using bioinformatics tools. To determine the codon optimization, the L1 protein sequence of HPV type 52 was retrieved from the National Center for Biotechnology Information (NCBI) database. Furthermore, codon optimization was conducted once the model had been established using OPTIMIZER, Clustal Omega, Restriction Mapper, and the ExPASy tool, based on L. lactis. The final construct was predicted to have a B-cell epitope by Ellipro tools, antigenicity by VaxiJen v.2.0, allergenicity by AllerTOP v.2.0, and toxicity by ToxIBTL. This resulted in a potential vaccine candidate with a Codon Adaptation Index (CAI) of 0.754 and GC content of 39.0%. Identification of epitopes from the optimized gene resulted in two peptide sequences, WRPSEATVYLPPVPVSKVVS and GTLGDPVPGDLYIKGSNSGNTATVQ, as components of the vaccine candidate.
Modulatory Effects of Fucoidan on Paracetamol-induced Hepatic Damage and Histological Alterations in Rasbora lateristriata Sofyantoro, Fajar; Dafa, Maula Haqul; Primahesa, Alfian; Oktaviana, Shintia; Mayani, Olvita; Simanungkalit, Eben Ezer; Prissandi, Anthera Al Firdaus; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry T. S. S. G.; Rohmah, Zuliyati; Widiyanto, Slamet; Wijayanti, Nastiti; Septriani, Nur Indah
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.27845

Abstract

Paracetamol overdose is a well-documented cause of hepatic injury across vertebrate species, including teleost fish. This study aimed to evaluate the hepatoprotective potential of fucoidan, a sulfated polysaccharide derived from brown algae, against paracetamol-induced liver damage in Rasbora lateristriata. Fish were divided into five experimental groups and exposed to paracetamol (3 mg/L), either alone or in combination with fucoidan at concentrations of 50, 100, or 300 µg/mL, for seven days. Histopathological evaluation of liver tissues was performed using hematoxylin–eosin staining, with semi-quantitative scoring focused on hydropic degeneration, nuclear pyknosis, and necrosis. The results demonstrated that paracetamol exposure induced moderate hepatocellular injury, characterized by cytoplasmic vacuolization, apoptotic nuclear alterations, and necrotic lesions. Co-treatment with fucoidan at 300 µg/mL was associated with reduced severity across all histopathological parameters, indicating partial hepatoprotective effects. In contrast, the 50 µg/mL fucoidan group exhibited paradoxically severe hydropic degeneration despite the absence of pyknosis and necrosis, suggesting a delayed or altered injury profile. Intermediate outcomes were observed at 100 µg/mL. Overall, fucoidan exhibited dose-dependent but inconsistent hepatoprotective effects. The observed histological variability across concentrations suggests that protection may be influenced by factors such as bioavailability, cellular uptake, or interactions with intracellular stress pathways. These findings highlight the need for further mechanistic investigations before fucoidan can be considered a reliable hepatoprotective agent in aquatic toxicology.
Histopathological Analysis of Liver Damage in Rasbora lateristriata Fish Induced by Extract Butterfly Pea Flower (Clitoria ternatea) Pertiwi, Anggita Endar; Illiyin, Sirayya; Sadewo, Imran; Rahayu, Sekar; Rumansara, Papuani Samparisna; Podhi, Felisitas Moli; Sularsah, Sais; Adelfiani, Adelfiani; Retnoaji, Bambang; Sofyantoro, Fajar; Nuriliani, Ardaning; Wijayanti, Nastiti; Saragih, Hendry T. S. S. G.; Rohmah, Zuliyati; Widiyanto, Slamet; Empra, Desi Eka Putri; Ariesti, Wiwin; Septriani, Nur Indah
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal (in process)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Butterfly pea flower (Clitoria ternatea) is an Indonesian native plant known for its medicinal properties due to its rich content of anthocyanins, flavonoids, and ternatins. Although widely recognized for its health benefits, the long-term effects of its bioactive compounds require careful evaluation. This study aimed to investigate the potential hepatotoxic effects of butterfly pea flower extract on the liver of Rasbora lateristriata fish. Fish were exposed to 0 (control), 50, 75, and 100 ppm of butterfly pea extract for four days, with four fish allocated per group. Histological markers, including necrosis, vacuolization, pyknosis, and hemorrhage, were assessed in liver tissues. Data were analyzed using the Kruskal-Wallis test and Mann-Whitney U test at a 95% confidence level. Results indicated that butterfly pea flower extract caused significant hepatic damage, with increased severity at higher concentrations. The most pronounced damage was observed at 75 ppm, suggesting an optimal dose for inducing oxidative stress. This study highlights the potential hepatotoxicity of butterfly pea flower extract in aquatic species and underscores the need for further research to evaluate its safety for therapeutic use.
Co-Authors . Sismindari Abdul Rahman Siregar, Abdul Rahman Abrory Agus Cahya Pramana Adelfiani, Adelfiani Adi Sofyan Ansori, Muhammad Aeniah, Siti Agustiningsih Agustiningsih Akira Hosoyama Allimi, Hayu Swari Amalia, Nur Rofika Ayu Shinta Andi Mahendra Andrian, Krisna Noli Anika Prastyowati, Anika Apriliyani, Tia Ardaning Nuriliani ARI SUSILOWATI Ariesti, Wiwin Arif Luthfi Nurul Huda Aris Haryanto Atsushi Yamazoe Aushia Tanzih Al Haq Bambang Hariwiyanto Bambang Retnoaji BUDI SAKSONO Camelia Herdini Ch. Retna Handayani Dafa, Maula Haqul Dafip, Muchamad Dewi Kartikawati Paramita Diani Mentari Dwi Liliek Kusindarta Dyah Widiastuti Empra, Desi Eka Putri ERLIN LISTIYANINGSIH Fajar Sofyantoro Hakim, Mohamad Saifudin Halim, Shuha Ma’muriyah Hanbal, Mayland Muhammad Hendry Tri Sakti Saragih Hera Nirwati Heriyanto, Didik S. Hevi Wihadmadyatami Hideaki Nojiri Ibnu Agus Ariyanto Illiyin, Sirayya Ina Kusrini Jajah Fachiroh, Jajah JAKA WIDADA Juliadmi, Dian Karlina, Ina Karnati, Srikanth Kartika W. Taroeno-Hariadi Kartika, Aprilia I. Lisna Hidayati Mayani, Olvita Mei Ria Santi Michael Kann Muhammad Adnan Mumtazah, Dzul Fithria Munawir Sazali Murwantoko . Naima, Mirtani Nenny Sri Mulyani Ni Wayan Erly Sintya Dewi Nisa, Mutia Khoirun Nuhamunada, Matin Oktaviana, Shintia Peni Indrayudha Pertiwi, Anggita Endar Pertiwi, Gian Aditya Podhi, Felisitas Moli Pradnya Paramita Pratitis, Visi Endah Primahesa, Alfian Prissandi, Anthera Al Firdaus Priyambada, Fajar Purnomo Hadi Pusparini, Nur Ainun Oktavia Puspitasari, Pinki Anggrahini Putra, Hendy Eka R.C. Hidayat Soesilohadi Rahayu, Sekar Ramadhani, Eka Rarastoeti Pratiwi Retno Murwanti Rinda Binugraheni, Rinda Rohmah, Zuliyati Rumansara, Papuani Samparisna Sadewo, Imran Saeed, Faisal Salamah, Rohmi Saragih, Hendry T. S. S. G. Sari, Ria Vinola Septhya Saribu, Ruth Liananda Citra Dolok Septriani, Nur Indah Septriani, Nur Indah Setiawibawa, Raden Aditya Aryandi Shinta Hartanto Simanungkalit, Eben Ezer Slamet Widiyanto Sofia M. Haryana Sofia Mubarika Haryana Subchan, Aditya Nur Sularsah, Sais Supraitno, Murtiadi Erlan Sutaryo1 S, Sutaryo1 T. Trijoko Tasania, Nadia Nisa Titis Widowati, Titis To'bungan, Nelsiani Tri Rini Nuringtyas Tri Wibawa Triska Desi Sundari Tsabitah, Khansa Wulansari, Riska Yundari, Yundari Zulfa, Amania Zulfadhli Zulfadhli Zumrotus Sholichah Zusrina, Laili Mufli ‘Aliyah, Siti Hamidatul