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All Journal Jurnal Teknik Industri Jurnal Kebidanan PROSIDING SEMINAR NASIONAL JURNAL LITBANG JURNAL KESEHATAN FIKkeS Jurnal Teknik ITS SURYA SENI Perspektif : Review Penelitian Tanaman Industri Rekayasa Sipil Jurnal Ilmiah Administrasi Publik (JI@P) Journal of Civil Engineering Fondasi: Jurnal Teknik Sipil PENELITIAN DAN KARYA ILMIAH The Southeast Asia Law Journal Prosiding Seminar Nasional Pakar Jurnal Pendidikan Sejarah dan Riset Sosial Humaniora (KAGANGA) Jurnal Ilmiah Ecobuss JTP - Jurnal Teknologi Pendidikan IJIES (International Journal of Innovation in Enterprise System) Menara Ilmu JURNAL MANAJEMEN DAN BISNIS INDONESIA Journal of Vocational Education Studies Buletin Palma Perspektif, Review Penelitian Tanaman Industri Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) El-kahfi : Journal of Islamic economics Jurnal Litbang Provinsi Jawa Tengah Charity : Jurnal Pengabdian Masyarakat EKONOMIKA45 JURNAL PENELITIAN DAN KARYA ILMIAH LEMBAGA PENELITIAN UNIVERSITAS TRISAKTI Sci-Tech Journal Jurnal Abdi Masyarakat Multidisiplin Edu Research : Jurnal Penelitian Pendidikan Jurnal Penelitian dan Karya Ilmiah Lembaga Penelitian Universitas Trisakti JURNAL TEKNIK INDUSTRI Media Ekonomi Concept: Journal of Social Humanities and Education Journal of Sustainable Development Issues Indonesian Journal of Science, Technology, and Humanities Tabsyir: Jurnal Dakwah Dan Sosial Humaniora Prosiding Seminar Nasional Unimus Observasi: Jurnal Publikasi Ilmu Psikologi Jurnal Pengabdian Masyarakat SAPANGAMBEI MANOKTOK HITEI PESHUM Paradigma: Jurnal Filsafat, Sains, Teknologi, dan Sosial Budaya
Budi Santosa
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Journal : PROSIDING SEMINAR NASIONAL

 1 
KARAKTERISASI MOLEKULER PROTEIN FLAGELLIN Salmonella typhi ISOLAT JAWA TENGAH DAN YOGYAKARTA Sri Darmawati; Budi Santosa; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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The purpose of this study is to molecularly characterize 7 strains of flagellin proteins of Salmonella typhi isolates taken from Central Java (5 strains from Semarang, 1 strain from Salatiga, 1 strain from Magelang) and 2 isolate strains from Yogyakarta (1 strain from Doctor Sarjito Hospital, and 1 strain from Bethesda Hospital). Flagellin gene (fliC) amplification is conducted using PCR (primer LPW 1856 and LPW 1857). Flagellin proteins resulted from Alexan method (2009) is then modified while flagellin protein profiles are obtained with SDS-PAGE method. The results show that the size of flagellin gene (fliC) of S. typhi Isolates from Salatiga and the 8 other strains is respectively 1262bp and 1500bp. Flagellin proteins which are composed of 2-6 protein sub-units consist of 1-2 major proteins and 1-4 minor proteins with the sizes of 16-116 kDa.Keywords: Salmonella typhi, flagellin, Molecular Characterizations
Rice Leaf Extract for Kidney Damage Prevention in Plumbum-Exposed Rats Budi Santosa; Henna Ria Sunoko; Andri Sukeksi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract. Plumbum (Pb) is a heavy metal affecting renal tubular epithelial cells and causing necrosis. This study aimedto demonstrate the effectiveness of rice leaf extract in degeneration and necrosis prevention of renal tubule epitheliumexposed to lead. Twenty eight rats were divided into a control and 3 treatment groups all exposed to Pb by 0.5 g/ kg/ day,but only the treatment groups had received rice leaf extract in varied doses for 8 weeks. Results from Kruskal-Wallis andsphearman rho tests showed that the number of normal cells, degeneration and necrosis were significantly different (0.03;0.05; 0.04) between control and treatment groups with a strong positive correlation of treatment (r = 0.97) in normal cellsand a strong negative one in cell degeneration and necrosis. (R = -0.92 and r = -0.93). As conclusion, rice leaf extractshowed ability in the prevention of kidney damage in rats exposed to lead.
Molecular Characterization And Hemagglutination Activities of Flagellin Protein of Salmonella typhi Sri Darmawati; Budi Santosa; Muhammad Evy Prastiyanto; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract. The purposes of this research are for molecular characterization and hemagglutination activity test of flagellinprotein of Salmonella typhi. The research samples consist of 7 strains of S. typhi isolates from Central Java (5 strains from Semarang city, 1 strain from Salatiga and 1 strain from Magelang) and 2 strains of S. typhi from Yogyakarta (Doctor Sardjito Hospital and Bethesda Hospital). The undertaking procedures are: 1) PCR and sequencing of fliC genes using primer LPW 1856 and LPW 1857.2) Isolation and separation of flagellin protein using SDS-PAGE. 3) Hemagglutination Activity Test upon human erythrocytes of blood group A, B, AB and O.The results show that 8 strains of S. typhi have a fliC gene size of 1452 to 1488 bp including serovar H1-d, and 1 strain with the size of 1267 bp including serovar H1-J. Flagella protein resulted from SDS-PAGE protein consists of 1-2 major proteins and 1-3 minor proteins with a molecular weight of 16-116 kDa. The results of hemagglutination activity test of flagellin protein show that there are 3 strains of S. typhi (MG-1, SA02.2 and BET) which are able to agglutinate human erythrocytes of bloodgroup A, B, AB and O (2-64HA), 6 other strains show various hemagglutination activities varied
ANALYSIS, IDENTIFICATION, AND FORMULATION OF METALLOTHIONEIN EXTRACTS ON NUMEROUS VARIETIES OF PADDY 95 LEAVES Budi Santosa; Henna Ria Sunoko
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Background: Vegetable materials, such as rice, corn, bean, and soybean have metallothionein proteins contained in their roots, stems, leaves, flowers, and fruits. Of those vegetable materials, paddy leaves have the most numerous metallotionein proteins. However, the varieties which produce the highest metallotionein proteins have not yet been found. The roles of metallothionein proteins are to bind and to detoxify heavy metals. Objectives:  to analyze, identify, and formulate the metallothionein extractscontained in numerous varieties of paddy leaves. An experimental research method. Each vegetable material of numerous varieties of paddy leaves is made into simplicia and is then processed into infusion. ELISA method is used as a technique to measure the protein level of each infusion. The metallothionein protein levels contained in numerous varieties of paddy leaves are then identified to determine the one with the highest metallothionein protein level. Extract formula is taken from the highest metallothionein level. Results :the highest metallothionein protein level is contained in paddy leaves of IR begendit (28.832 ng) while the lowest one is in paddy leaves of IR-64 SS (0.358 ng). The extracts of Paddy leaves of IR begendit with the highest protein level are then formulated into metallothionein extracts. Conclusion: Analysis on metallothionein levels of numerous varieties of paddy leaves with the highest protein level is contained in paddy leaves of IR begendit (28.832 ng) while the lowest one is in paddy leaves of IR-64 SS (0. 358 ng).Keywords: paddy leaves, metallothionein
DIFFERENTIAL COUNTING BERDASARKAN ZONA BACA ATAS DAN BAWAH PADA PREPARAT DARAH APUS Budi Santosa
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2010: Bio Molekuler, Analis Kesehatan, Keperawatan
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Latar belakang: Differensial counting merupakan hitung jenis lekosit  dilakukan bersama-sama dengan pemeriksaan apus darah tepi. Bentuk preparat darah apus adalah simetris sehingga dimungkinkan distribusi yang sama atau hampir antara zona atas dan bawah. Penelitian ini bertujuan untuk mengetahui perbedaan antara zona atas dan bawah sehingga pembacaan pada zona IV sampai VI dapat dilakukan guna menghitung 100 sel lekosit.Metoda: Penelitian dilaksanakan di Laboratorium Patologi Klinik Unimus antara bulan April sampai Juni 2009. Jenis penelitian ini adalah analitik  dengan cara membandingkan hasil hitung jenis sel lekosit berupa basofil, eosinofil, staff, segmen dan limfosit pada zona atas dan bawah preparat darah apus. Sampel diperoleh secara purposif dari mahasiswa D III Analis Kesehatan sebanyak 10 orang berupa darah vena.  Masing-masing sampel dibuat preparat apus duplo menggunakan pengecatan Giemsa. Pembacaan preparat darah apus dilakukan pada zona atas dan bawah masing-masing sebanyak 100 sel lekosit. Dibandingkan rerata zona atas dan bawah, analisis data menggunakan analisis statistik dengan program komputer SPSS. Teknik analisis data untuk mengetahui perbedaan jenis-jenis lekosit yang berupa basofil, eosinofil, staff, segmen dan limfosit menggunakan analisis t test relatid / Wilcoxon.Hasil penelitian: tidak terdapat perbedaan hasil penghitungan jenis-jenis lekosit antara zona atas dan bawah.Simpulan: hitung jenis lekosit dapat dilakukan pada salah satu bagian yaitu atas atau bawah zona IV sampai VI. Kata kunci: Differential counting, zona atas dan bawah
Co-Authors Abshar Ramadhanny Subhan Ade Lia D. Safitri ade rahmawati Afrinaldi Afrinaldi Agus Alex Yanuar Agustin Rahmawati Ahmad Fauzan Abdurrahman Ahmad Yazid Lubis Aisyah Aji Sukarno Alfi Rahmi Alfita Ayu Irmawati Ali Ridho Barakbah Andika Arbi Rayhansyah Andri Sukeksi Anita Anita Annisa Nurul Hikmah Antoni Antoni Ari Yanuar Ridwan Aris Budi Setyawan Azzati Nur Laili Bagus Nurcahyo Bambang Utoyo Cakti Indra Gunawan Dassaad, Dassaad Didik Sumanto Ditiya Himawati Donata S. Pandin Dwi Aries Himawanto Dwi Sulistyoningsih Dyah Palupi Eka Puji Hastuti Fadhilla Yusri Fasnia Septa Utama Fatwa Tentama Fitriani Nur Damayanti Hafiz Mukaromah Hani Harlan HARYONO Haryono Haryono Haryono Haryono Hengky Novarianto Henna Ria Sunoko Herlisa Anggraini Herminarto Sofyan Hidayani Syam I Nyoman Pujawan I Wayan Arsana Iman Pasu Marganda Hardianto Purba Ismail Maskromo Iswantir M Joko Budi Santosa Joko Utomo Ketut Purwantoro Lasminiasih Linda Yarni M. Arif Maria R Nindita Radyati Martino Wibowo Masnia Mahardi Yanuar MAYA MUSTIKA KARTIKA SARI, MAYA Megandaru Widhi Kawuryan Mei Raharja Meisin Nabilah Meity Aneke Tulalo Meity Aneke Tulalo Meldy Hosang Meutia Srikandi Fitria Muh Ilham Muhammad Evy Prastiyanto Muji Rahayu Nidia Fertisia S Niken Anggraini Savitri Nur Cholidah Fitriana Nurdiansyah Rudi NURUL QOMARIYAH Nuryani Nuryani Nuzul Husnah Oksiana Jatiningsih Prasetyo Bonifasius Sitanggang Prihandoko Rachmi Ridho Ragil Saptaningtyas Rahmanu Wijaya Rahmi Andayani Ranny Wahyu Ningrat Ratih Juwita Rehulina Apriyanti Rina Sugiarti Rini Megawati Rino Andias Anugraha Rio Aurachman Risma Widia Ramadhanti Riyanti Riyanti Rizka Nindya Wulandari Rizka Rahmadani Rizki Muti Agustiani Sampara Lukman Sarmini Saskia Dyah Satria Mandala Sid Suntrayuth Sidi Asmadi SITI AISAH Soeharjoto Soeharjoto Sri Astuti Eka Putri Sri Darmawati Sri Nawangsari Stanty Aufia Rachmat Stanty Aufia Rahmat Stefanus Eko Wiratno Steivie Karouw Sudarsono, Bambang Sukarno, Aji Sukmawati Mawardi Sumiyarti Sumiyarti Supriyadi Supriyadi Suyanto Suyanto SUYONO Syawaluddin Syawaluddin Tahta Alfina Tertio Kunto Dewo Tiananda Widyarini Tri Mulyani Wahyuningsih Umar Affandi Veronika Widi Prabawasari Waenah . . Wahyu Prakosa Wahyu Sunan Hidayat Wardoyo Warsono Willy Arafah Wiyono Sutari Yanto Sidik Pratiknyo Yuli Sari Yeni Yulia Rosyada, Fani YULIANUS R. MATANA Zela Ubaidillah