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Effectiveness of the Combination of Spirulina platensis and Stichopus variegatus on Prevention of Caspase-3 Gene Expression of Dementia Rat Models Safira, Rizka; Botutihe, Lisa Agustina; Yuliani, Sapto; Kintoko, Kintoko
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 21, No 1 (2024)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.006051

Abstract

The death of cells in the brain, especially the hippocampus, manifests in a decrease in memory, language, and behavior. Golden sea cucumber (Stichopus variegatus) andblue-green algae (Spirulina platensis) are reported to have high antioxidant content which has the potential to prevent cell death in the brain due to oxidative stress thereby preventing memory decline.This study aimed to investigate the effect of the combination of Stichopus variegatus and Spirulina platensis on preventing caspase 3 gene expression in pyramidal hippocampal cells in a rat model of trimethlytin-induced dementia (TMT). This study used Sprague Dawley rats, about 2 months old, weighing 180-200 g, divided into 6 treatment groups, with each group consisting of 8 rats. The hippocampus was taken from the right cerebral hemisphere for histological observations of pyramidal caspase-3 gene expression in the CA1 and CA2-CA3 regions.The combination of Spirulina platensis and Stichopus variegatus with a dose 200 mg/kg BB can prevent the expression of caspase-3 in the CA1 and CA2-CA3 areas of the hippocampus. Conclusion: The combination of spirulina and golden sea cucumber extracts has the potential to prevent caspase 3 gene expression in pyramidal cells of TMT-induced dementia rat models. 
Pendampingan Pelaksanaan Proyek Penguatan Profil Pelajar Pancasila (P5) dengan Tema Kearifan Lokal Kintoko, Kintoko; Rohmah, Lely Arbiati Nur; Warniasih, Kristina
Prima Abdika: Jurnal Pengabdian Masyarakat Vol. 3 No. 4 (2023): Volume 3 Nomor 4 Tahun 2023
Publisher : Program Studi Pendidikan Guru Sekolah Dasar Universitas Flores Ende

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/abdika.v3i4.3340

Abstract

The Pancasila Student Profile is one of the efforts that is expected to improve the quality of education in Indonesia, one of which is by developing the character of students. Character development is very necessary for students to build highly intellectual people. The media used to develop student character is the implementation of the Pancasila Student Profile Strengthening Project. This service aims to provide insight and description of the implementation of the Strengthening Pancasila Student Profile (P5) Project with the theme of local wisdom in junior high schools to realize the implementation of the independent curriculum. This service activity method is carried out through observation and mentoring. The results of the activity show that students' knowledge about the local wisdom of traditional games in the surrounding area has increased and the moral character of students in junior high school can also be developed.
Fermentasi Poliherbal TBR Terhadap Kandungan Flavonoid Total dan Aktivitas Antioksidan serta Berat Badan Secara In Vivo Atmarani, Rizki Essa; Kintoko, Kintoko; Sulistyani , Nanik; Taufik, Epi
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i3.57968

Abstract

Minuman fermentasi diketahui memiliki efek yang dapat meningkatkan kadar flavonoid total dan antioksidan,serta dapat mempengaruhi berat badan yang disebabkan oleh reaksi dari bakteri asam laktat. Antioksidan pada tanaman, Taxus Sumatrana (cemara sumatra), bajakah jixueteng (spatholobus suberectus) dan rumput mutiara (Hedyotis corymbosa) dilaporkan memiliki kandungan flavonoid total dan kadar antioksidan tinggi. Penelitian ini bertujuan untuk membuat sediaan minuman fermentasi poliherbal,serta mengukur kadar flavonoid total dan antioksidan Yoghurt Polihrerbal TBR (3:1:1) serta mengetahui pengaruhnya terhdap penurunan berat badan dan indeks organ hewan uji. Metode ekstraksi yang digunakan dalam penelitian ini adalah metode maserasi dengan menggunakan pelarut etanol 96% selama 1x24 jam, selanjutnya dilakukan pengujian flavonoid total menggunakan baku standar kuersetin, setelah itu difrementasi menggunakan Bakteri Asam Laktat (BAL) dengan konsentrasi ekstrak TBR (3:1:1) 1%, 2% dan 3% , lalu pengujian antioksidan dengan metode ABTS serta pengukuran berat badan dan indeks organ pada tikus putih (Rattus novergicus) dengan 7 kelompok perlakuan berbeda. Analisis data penelitian ini menggunakan data komputasi dan statistik menggunakan softwere SPSS VER 2.0.
Efektivitas Model Pembelajaran Think Talk Write (TTW) Terhadap Kemampuan Literasi Numerasi Matematika Siswa Kelas VIII Oktavia, Lisa; Susetyawati, MM Endang; Kintoko, Kintoko
Indo-MathEdu Intellectuals Journal Vol. 5 No. 1 (2024): Indo-MathEdu Intellectuals Journal
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v5i1.761

Abstract

The TTW learning model is a discovery model which includes thinking, speaking, and writing activities. This research was conducted with the aim of seeing the effectiveness of the TTW learning model in terms of the mathematics numeracy literacy abilities of class VIII students. This quantitative research uses a Quasi Experimental method with a one group posttest design. The population in this study were all class VIII students of SMP N 1 Bambanglipuro. The technique used is simple random sampling to determine the control class and experimental class. Class VIIIF has 27 students as the control class and class VIIIA has 27 students as the experimental class
Analisis Deskriptif Kompetensi Pedagogis Calon Guru: Membangun Pondasi Pendidikan yang Kuat Susetyawati, MM. Endang; Kintoko, Kintoko
Indo-MathEdu Intellectuals Journal Vol. 6 No. 1 (2025): Indo-MathEdu Intellectuals Journal
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v6i1.2480

Abstract

Effective lesson planning can guide the teaching and learning process to become more efficient, provide relevant learning experiences tailored to students' needs, and ensure the achievement of learning objectives. This study aims to analyze the ability of prospective elementary school teachers in designing lesson plans and implementing lessons in elementary schools. This research employs a descriptive qualitative approach, focusing on teacher certification program (PPG) participants who are prospective elementary school teachers. Data were collected through an analysis of lesson planning documents and classroom observation of lesson implementation. The data analyst technique is carried out with three steps of qualitative data analysis in the form of data reduction, data presentation and verification or drawing conclusions. The findings indicate that prospective elementary school teachers are capable of preparing well-structured lesson plans and executing lessons effectively according to their plans. This study suggests that both prospective and active teachers should continuously improve their pedagogical competence to support the success of the teaching and learning process in elementary schools.
Pengaruh Pendekatan Open Ended Terhadap Kemampuan Berpikir Kritis Matematika Siswa Nurlita, Tasya; Kintoko, Kintoko; Aviory, Koryna; Susetyowati, MM Endang; Sunanti, Titis
Indo-MathEdu Intellectuals Journal Vol. 6 No. 1 (2025): Indo-MathEdu Intellectuals Journal
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v6i1.2494

Abstract

The purpose of this study is to describe students' critical thinking skills using the open ended approach and using the Scientific approach and to determine the effect of the open ended learning model on students' critical thinking skills. The research method used is a quasi-experimental design with a nonequivalent control group design with a population taken from class VIII A and B of SMP Masyithoh Kroya, Cilacap Regency. This research was conducted in September 2024. This study uses a quantitative research method. Data collection uses test, observation and documentation techniques. Validity uses professional judgment techniques. The data analysis technique uses the Mann-Whitney test after the analysis prerequisite test includes the normality test of distribution and the homogeneity test of variance. Based on the analysis carried out by the researcher, it can be concluded that the critical thinking skills of students in the experimental class after applying the open ended approach model got an average score of 36.62, and the control class using the Scientific approach got an average score of 28.39. The use of the open-ended approach model has a significant effect on students' critical thinking skills, this is proven by the results of the Mann-Whitney test which shows a high Asymp value. Sig.(2-tailed) = 0.000, which is less than the significance level of 0.05, from these results it is concluded that the Pythagorean open-ended approach has a significant effect on students' critical thinking skills in mathematics.
Uji Aktivitas Antibakteri EkstrakEtanol dan Fraksi Batang Kuning (Fibraurea Tinctoria Lour) terhadap Bakteri Escherichia coli dan Staphylococcus aureus Ariyanti, Lusi Mardika; Supomo, Supomo; Sa’adah, Hayatus; Syamsul, Eka Siswanto; Kintoko, Kintoko; Witasari, Hardi Astuti
JI-KES (Jurnal Ilmu Kesehatan) Vol. 5 No. 2 (2022): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : LPPM Universitas Hafshawaty Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/ji-kes.v5i2.323

Abstract

Abstrak Akar kuning (Fibraurea tinctoria Lour) merupakan tumbuhan khas yang dapat dijumpai di Kalimantan serta biasa dimanfaatkan oleh masyarakat setempat sebagai obat gatal, penyakit kuning dan diare. Salah satu senyawa kimia yang terkandung dalam akar kuning adalah berberin yang berpotensi memiliki khasiat sebagai antidiabetes, antivirus, antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk  mengetahui potensi tumbuhan Akar Kuning (Fibraurea tinctoria Lour.) sebagai antibakteri terhadap bakteri Escherichia coli dan Staphylococcus aureus dengan menggunakan metode disc diffusion. Ekstrak etanol difraksinasi dengan menggunakan pelarut n-heksan dan etilasetat, ekstrak dan fraksi yang telah didapat ditimbang dengan konsentrasi 2,5%, 5% dan 10%. Kontrol positif yang digunakan yaitu amoxicillin dan DMSO sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa ektrak etanol dan fraksi akar kuning (Fibraurea tinctoria Lour.) memiliki potensi untuk menghambat pertumbuhan bakteri. Aktivitas zona hambat terbesar yang terbentuk pada ekstrak etanol yaitu pada konsentrasi 10% dengan diameter zona hambat 9,18 mm terhadap bakteri Escherichia coli dan 12,16 mm terhadap bakteri Staphylococcus aureus sedangkan fraksi batang akar kuning yang memiliki aktivitas antibakteri paling kuat terhadap bakteri Escherichia coli dan Staphylococcus aureus yaitu fraksi sisa. Staphylococcus aureus memiliki sensitifitas lebih tinggi terhadap akar kuning (Fibraurea tinctoria Lour) dari pada bakteri Escherichia coli. Kata kunci  : antibakteri, akar kuning, berberin, Fibraurea tinctoria Lour. Abstract Akar kuning (Fibraurea tinctoria Lour.) is typically plant that can be found in Kalimantan usually used by local people as itch medicine, jaundice and diarrhea. Berberin, one of the chemical compounds contained in the akar kuning, berberin has the potential to act as an anti-diabetic, antiviral, antibacterial, and anti-inflammatory. This study aimed to determine the activity of akar kuning as an antibacterial against Escherichia coli and Staphylococcus aureus using the disc diffusion method. Ethanol extract are fractionated using n-Heksan and Etilasetat solvents, the obtained of extract and fraction are weighed to 2,5%, 5% and 10%. Positive control antibacterial used amoxicillin and DMSO as negative control. The results showed that ethanol extracts and fraction of akar kuning (Fibraurea tinctoria Lour.) are have potential to inhibited bacteria growth. The highest antibacterial activity that showed at 10% concentration of ethanol extract with diameter inhibition is 9,18 mm to Escherichia coli and 12,16 mm to Staphylococcus aureus while the fraction of akar kuning which has the stronger antibacterial activity to Escherichia coli and Staphylococcus aureus is the rest fraction. Staphylococcus aureus were more susceptible to akar kuning (Fibraurea tinctoria Lour.) extract and fraction than Escherichia coli.Keywords:           antibacterial, akar kuning, berberin, Fibraurea tinctoria Lour
ETNOMEDICINE STUDY: KATUK LEAVES (SAUROPUS ANDROGYNUS (L.) MERR.) FOR BREAST MILK BOOSTER IN SUMBERAN NGESTIHARJO KASIHAN BANTUL Sri Budiarti, Nur Idha; Kintoko, Kintoko
International Journal of Islamic and Complementary Medicine Vol. 2 No. 2 (2021): International Journal Islamic Medicine
Publisher : International Islamic Medicine Forum

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.844 KB) | DOI: 10.55116/IJIM.V1I1.21

Abstract

Breastfeeding is very important for optimal growth and development for babies. One of the efforts that can be done to increase breast milk production is by using traditional ingredients such as katuk extract (Sauropus androgynus (L.) Merr.). This study aims to determine form of the herb, processing method, usage method and the factors behind people using katuk leaves (Sauropus androgynus (L.) Merr.) for breast milk booster in Sumberan Ngestiharjo Kasihan Bantul. The research method used is descriptive qualitative observation. The sampling technique was snowball sampling through observation and in-depth interviews with informants who knew about the use of katuk plants to increase breast milk production in Sumberan Village, then the informants selected respondents to provide additional information. The results showed that the form of katuk leaves (Sauropus androgynus (L.) Merr.) was as a vegetable, brewed, encapsulated and blended to extract the starch. The processing method is cooked as a vegetable, made juice to drink, boiled for fresh vegetables and dried, its use by eating with a frequency of two times a day used during breastfeeding. The factors behind people using katuk leaves to increase breast milk production that is believed katuk leaves can increase breast milk production or breast milk boosters at low prices and easy to obtain. Katuk leaves (Sauropus androgynus (L.) Merr.) to increase breast milk production, which can be consumed as a vegetable by eating it with a frequency of two times a day during breastfeeding. The factors behind people using katuk leaves (Sauropus androgynus (L.) Merr.) that katuk leaves are believed to increase breast milk production.  
Place-based ethnomathematics for early numeracy: Geometric representations from the tugu pahlawan monument in Surabaya Yustitia, Via; Kintoko, Kintoko; Pamungkas, Megita Dwi; Wijayanti, Septiana
Al-Jabar: Jurnal Pendidikan Matematika Vol 16 No 2 (2025): Al-Jabar: Jurnal Pendidikan Matematika
Publisher : Universitas Islam Raden Intan Lampung, INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/ajpm.v16i2.29234

Abstract

Purpose: This study aims to understand how the cultural elements of Surabaya’s Tugu Pahlawan Monument can be used to introduce basic numeracy in primary school, especially concepts related to plane geometry. The goal is to show that cultural landmarks can offer real and relatable examples for young learners. Method: The research was conducted through a qualitative ethnographic approach. The researcher observed the monument directly, spoke with on-site guides and staff who were familiar with its history, and documented various architectural and visual features. All information gathered from notes, photographs, and short interviews was analyzed using the Miles and Huberman framework to identify patterns that relate to mathematical ideas. Findings: The study found that many parts of the monument display clear geometric forms. These include rectangles, squares, circles, triangles, trapezoids, rhombuses, and even a ten-sided figure. Such shapes appear in the floor patterns, plaques, murals, decorative ornaments, museum structures, and the wheels of military displays. Each of these elements can be directly connected to geometry topics taught in primary classrooms. Significance: The findings highlight the potential of local heritage as a meaningful resource for mathematics education. When students see that the shapes they learn in class appear in a monument tied to their own city’s history, mathematical concepts become easier to grasp and more engaging. This approach supports early numeracy while also helping learners build a stronger connection to their cultural environment.
Emulgel Formulation of Purified Extract of Moringa (Moringa oleifera L.) Leaf Wuryandari, Tutik; Sugihartini, Nining; Kintoko, Kintoko
Folia Medica Indonesiana Vol. 55, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Moringa oleifera, known as moringa, is reported to have a high source of natural antioxidants, especially vitamin E. The formulation of purified Moringa leaf extract is needed so that the vitamin E content in it is more maximal. This study aimed to determine the levels of vitamin E in extracts after purification, physical property test of emulgel with variations in extract concentration and vitamin E levels in emulsions. The study was started from the extraction using maceration method with petroleum ether solvent, followed by extract purification using column chromatography with n-hexane solvent. The results of the purified extract were then formulated into emulgel with three concentrations of 3% (F1), 6% (F2) and 9% (F3). Emulgel was then evaluated for physical characteristics (pH, viscosity, adhesion, dispersion and stability). Determination of vitamin E levels was carried out on purified extract samples and on each formula. The results of non-specific standardization of extracts included ash content, acidic insoluble ash content were higher than those of Indonesian Herbal Pharmacopoeia literature and drying losses were lower compared to literature. Specific standardization of extracts was the content of vitamin E in 1.40% PE extract and 8.02% purified extract. In the test of emulgel physical characteristics, an increase in the concentration of the extract caused an increase in viscosity and decreased emulgel dispersion power. Whereas, tthe pH and sticky power remained the same. Storage hds an effect on decreasing the physical characteristics of emulsions especially on adhesion. Increased extracts also caused an increase in the levels of vitamin E in the purified extract of moringa leaf emulgel.
Co-Authors Alfarisyi, Muhammad Iqbal Ananda, Aghnia Nabila Anggraeni, Linda Suci Ari Satia Nugraha Aris Setyawan Ariyanti, Lusi Mardika Asmah, Richatul Astuti, Andarweni Atmarani, Rizki Essa aviory, koryna Ayu Shabrina Billy Harnaldo Putra, Dini Fatmi, Botutihe, Lisa Agustina Cahayani, Devita Mika Desmayanti, Astri Dhian Arista Istikomah Dian Aprilia, Dian Dona, Suzana Dwi Pamungkas, Megita Dwi Utami Eka Siswanto Syamsul Eko Andy Purnomo Epi Taufik Faisal Nur Arib Fajar Fajar Farid , Muhammad Ferani Mulianingsih Gunawan Gunawan Gunawan, Adhi Gusriyani, Sri Hadi Siswanto, Deny Hardi Astuti Witasari Harry Ade Saputra Hasana, Siti Nur Hayatus Sa`adah Hutari, Andri Iis Wahyuningsih Ilmia, Nisrien Indahsari, Kharisma Safitri Nur Jana, Padrul Khrisna Agung Cendekiawan Khusnul, Latifah Kusumo, Djati Wulan Lestariningrum, Wahyu Titis M. Yogi Riyantama Isjoni Maghfirah, Yolanda Sajjida Makati, Annisa Camellia Martyana Prihaswati Maulizah, Rizlah Mehta, Kunal Mochammad Saiful, Bachri Muhammad Iqbal Birsyada Muldiyana, Tya Mustofa Ahda Mutti, Annisa Aulia Nanang Khuzaini, Nanang Nanik Sulistyani Nina Salamah Nina Widyaningsih Ningrum, Lailatul Fitria Nining Sugihartini Novitasari, Putri Rachma Nur Arib, Faisal Nurkhasanah Mahfudh Nurlita, Tasya Nurmawati Nurmawati Nuryani, Christina Eva Oktavia, Lisa Pamungkas, Megita Dwi Pulhehe, Hasni Rangkuti, Ahmad Faizal Ranti, Imaniar Rejeki, Marsudi Endang Sri Restu Ayu Mumpuni, Restu Ayu Ridho, Anisa Rifka Rifki Febriansah Rohma, Siti Rohmah, Lely Arbiati Nur Rukaya, Benazir Evita Safira, Rizka Santoso, Blegoh Iwan Sapto Yuliani Saputri, Ginanjar Zukhruf Septian Aji Permana Septiana Wijayanti Setiyawati, Fitri Indah Siswanto, Deny Hadi Siti Suprihatiningsih Sri Budiarti, Nur Idha Suhendra Suhendra Sulistyani , Nanik Sulistyowati, Ani Sunanti, Titis Supomo Supomo Susetyawati, MM Endang Susetyawati, MM. Endang Susetyowati, MM Endang Tri Puspita Yuliana Triyulia, Ghea Nanda Urmatul Waznah Utami, Febri Venissa Dian Mawarsari Via Yustitia Wahyuni, Dina Wardhani, Firdha Aprillia Warniasih, Kristina Widyaningrum, Rachmawati Wuryandari, Tutik Wuryandari, Tutik Yanti, Asti Afrida Yuli Nurullaili Efendi, Yuli Nurullaili Yulia Yesti, Yulia Yulianto Yulianto Yuni Suprapto, Yuni