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Journal : Aceh Journal of Animal Science

Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum Septian Hakim Susantoputro; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Heni Puspitasari
Aceh Journal of Animal Science Vol 5, No 2 (2020): Desember 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.2.16780

Abstract

The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody  .
Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla