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All Journal Asian Journal of Community Services (AJCS) Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Buletin Pengabdian
Mulyadi Adam, Mulyadi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Armansyah, Teuku; Siregar, Tongku Nizwan; Aliza, Dwinna; Melia, Juli; Meutia, Nellita; Panjaitan, Budianto; Hafizuddin, Hafizuddin; Adam, Mulyadi; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattles estrous cycle were synchronized by 5 mg/mL of PGF2 intramuscularly (Lutalyse, Pharmacia Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicles growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattles follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Wahyuni, Sri; Agungpriyono, Srihadi; Adnyane, I. Ketut Mudite; Hamny, Hamny; Jalaluddin, Muhammad; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Aliza, Dwinna; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 g/ml of concentration for PNA lectins and 15g/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis.Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit) G, Gholib; Wahyuni, Sri; Kadar, Okta Hilda; Adam, Mulyadi; Lubis, Triva Murtina; A, Azhar; Akmal, Muslim; Siregar, Tongku Nizwan; Armansyah, Teuku; Nugraha, Taufiq Purna
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3367

Abstract

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (2 years), six kids (6 months), and six does (2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.654.27%, 10%, 15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat
PEMBERIAN EKSTRAK EPIDIDIMIS BERPOTENSI MENINGKATKAN KUALITAS SPERMATOZOA KAMBING JANTAN LOKAL Akmal, Muslim; Siregar, Tongku Nizwan; Wahyuni, Sri; Hambal, Muhammad; S, Sugito; A, Amiruddin; S, Syafruddin; R, Roslizawaty; Z, Zainuddin; Adam, Mulyadi; G, Gholib; Iskandar, Cut Dahlia; R, Rinidar; Asmilia, Nuzul; H, Hamny; J, Joharsyah; S, Suriadi
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2839

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak epididimis (EE) terhadap peningkatan kualitas spermatozoa kambing jantan lokal. Dalam penelitian ini digunakan 12 ekor kambing jantan lokal, berumur 1,5 tahun dengan bobot badan 10-15 kg dan dibagi atas empat kelompok (K0, KP1, KP2, dan KP3). Kelompok K0, hanya diinjeksi dengan NaCl fisiologis sedangkan kelompok KP1, KP2, dan KP3 diinjeksi EE masing-masing 1, 2, dan 3 ml/ekor selama 13 hari berturut-turut. Pada hari ke-14, dilakukan pengambilan semen kambing dengan elektroejakulator dan selanjutnya dilakukan pemeriksaan kualitas spermatozoa. Hasil penelitian menunjukkan bahwa pemberian EE dengan dosis 1 dan 3 ml/ekor EE selama 13 hari berturut-turut menyebabkan peningkatan kualitas spermatozoa dibanding kelompok kontrol. Berdasarkan hal tersebut dapat disimpulkan bahwa EE berpotensi meningkatkan kualitas spermatozoa pada kambing jantan lokal.
INHIBIN B MENURUNKAN KONSENTRASI FOLLICLE STIMULATING HORMONE (FSH) PADA TIKUS PUTIH (Rattus norvegicus): UPAYA PENGEMBANGAN KONTRASEPSI HORMON PRIA BERBASIS PEPTIDA Akmal, Muslim; A, Aulanniam; Widodo, M. Aris; Sumitro, Sutiman B.; Purnomo, Basuki B.; Siregar, Tongku Nizwan; Hambal, Muhammad; A, Amiruddin; S, Syafruddin; Aliza, Dwinna; Sayuti, Arman; Adam, Mulyadi; Armansyah, T.; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2788

Abstract

Penelitian ini bertujuan mengetahui efek injeksi inhibin B terhadap penurunan konsentrasi follicle stimulating hormone (FSH) di dalamserum pada tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 24 ekor tikus putih berjenis kelamin jantan dengan strain Wistar berumur 4 bulan dengan bobot badan 150-200 g. Tikus-tikus dikelompokkan secara acak ke dalam 4 kelompok, yaitu KK0, KP1, KP2, dan KP3, masing-masing kelompok terdiri atas 6 ekor. Kelompok KK0 merupakan kelompok kontrol hanya diinjeksi dengan phosphate buffer saline (PBS), sedangkan kelompok KP1, KP2, dan KP3 diinjeksi dengan inhibin B dengan dosis berturut-turut 25, 50, dan 100 pg/ekor. Injeksi inhibin B dilakukan secara intraperitoneum sebanyak 5 kali selama 48 hari dengan interval waktu 12 hari. Injeksi pertama inhibin B dilarutkan dengan0,05 ml PBS dan 0,05 ml Freuds complete adjuvant (FCA). Injeksi kedua sampai kelima, inhibin B dilarutkan dengan 0,05 ml PBS dan 0,05 ml Freuds incomplete adjuvant (FICA). Pada hari ke-6 setelah injeksi inhibin B terakhir, tikus dikorbankan secara dislocatio cervicalis,lalu darah dikoleksi langsung dari jantung dan didiamkan hingga didapatkan serum untuk pemeriksaan konsentrasi FSH dengan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa injeksi inhibin B dengan dosis 100 pg/ekor menurunkan konsentrasi FSH secara nyata (P0,05) bila dibandingkan dengan kelompok kontrol. Berdasarkan hal tersebut, inhibin B berpeluang untuk dikembangkan sebagai kandidat kontrasepsi pria hormon berbasis peptida.
Enhancing the Capacity of Certified SMEs Facilitators for the Internationalization of Aceh's Micro, Small, and Medium Enterprises Halim, Hendra; Kesuma, T. Meldi; Siregar, M. Ridha; Faisal, Faisal; Darwanis, Darwanis; Majid, M. Shabri Abdul; Fadhil, Rahmat; A Sakir, A Sakir; Syahrizal, Teuku Muhammad; Umuri, Khairil; Sitepu, Novi Indriyani; Nurdin, Ridwan; Sari, Meutia Dwi Novita; Adam, Mulyadi; Khairi, Fitrah
Asian Journal of Community Services Vol. 3 No. 1 (2024): January 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/ajcs.v3i1.7752

Abstract

This community services aims to enhance the capabilities of certified SMEs facilitators from the Faculty of Economics and Business, Syiah Kuala University to internationalizing Aceh's Micro, Small, and Medium Enterprises (SMEs) through participation in the 19th Malaysia International Halal Showcase (MIHAS) 2023. That is not merely a journey to MIHAS; it is a strategic investment in building the competencies of facilitators who, in turn, become ambassadors of knowledge, aiding local SMEs enterprises in embracing internationalization with confidence and competence. Results showcase strengthened international networks and market reach, emphasizing the impactful role of university-community collaborations.
Klinik Hewan Keliling Rumah Sakit Hewan Pendidikan Prof. Noerjanto Universitas Syiah Kuala [Mobile Veterinary Clinic of Prof. Noerjanto Hospital Teaching Veterinary Hospital of Universitas Syiah Kuala] Sayuti, Arman; Panjaitan, Budianto; Syafruddin, Syafruddin; Adam, Mulyadi; Thasmi, Cut Nila; Roslizawaty, Roslizawaty; Rosmaidar, Rosmaidar; Siregar, Tongku Nizwan; Armansyah, Teuku; Sutriana, Amalia; Aliza, Dwinna
Buletin Pengabdian Vol 4, No 2 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v4i2.37290

Abstract

The long-term purpose of this community service is to support the autonomy of the university through independent income generation and to encourage the development of a culture of utilizing university research results for the community through the mobile veterinary clinic business carried out by Prof. Noerjanto Teaching Veterinary Hospital. To achieve above objectives, it was necessary to modify the existing clinic vehicle to include an operating room, mini-laboratory and pharmacy. The implementation of activities was carried out for five working days a week, by actively visiting smallholder livestock centers per village per day in the Aceh Besar areas. Services provided included livestock health consultation, treatment, vaccination (an effective measure as a form of protection for animals, synchronization of heat and artificial insemination, and assistance during birth. The diagnosis methods utilize the latest techniques, namely diagnosis and therapy techniques used in the field of veterinary medicine, which were the results of previous research. Mobile Clinic of Prof. Noerjanto's Teaching Veterinary Hospital had been modified by providing an operating table, mini laboratory, pharmacy, and repainting the car, thus increased the attractiveness of the community during the activities were carried out. Mass treatment of livestock in Aceh Besar district had reached the target which services covered five sub-districts. In conclusion, the mobile veterinary clinic in 2017 has successfully modified the clinic car and provided health services in 5 sub-districts in Aceh Besar District.
Pemanfaatan larutan lugol 2% untuk menanggulangi kasus kawin berulang pada sapi di Gampong Cot Madhi, Aceh Besar (Utilization 2% lugols solution to overcome the repeat breeding case of cows in Cot Madhi Village, Aceh Besar) Syafruddin, S; Adam, Mulyadi; Roslizawaty, R; Siregar, Tongku Nizwan; Hafizuddin, H; Panjaitan, Budianto; Armansyah, Teuku; Wahyuni, Sri; Sayuti, Arman
Buletin Pengabdian Vol 1, No 2 (2021): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v1i1.20108

Abstract

The objective of this activity was to introduce the use of Lugol's solution to farmers in Cot Madhi Village as an alternative solution to overcome the repeat breeding case in cows. This activity has involved 10 farmers who have cows suspected of experiencing repeat breeding in the village according to the information from the local inseminator. The implementation of this activity was carried out by two methods, namely (1) education and counseling methods and (2) demonstration and field training methods. From these community service activities that have been carried out, farmers have realized the importance of handling reproductive cases to increase livestock productivity. In addition, participants also understood how to obtain Lugol's solution in the market, did dilution according to the recommended dose, and understood the technical treatment of repeat breeding in cows using Lugol's solution. In conclusion, participants have understood the technical treatment with Lugol's solution.
Herbal flavonoids in preserving testicular physiology under oxidative stress: A bibliometric analysis of systematic reviews Zulkifli, Baidillah; Fakri, Fajar; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Sabri, Mustafa; Lubis, Triva Murtina; Zainuddin, Zainuddin; Zulfitri, Zulfitri; Admi, Masda; Syah, Nanda Yulian; Usrina, Nora; Fajri, Fauzan
The International Journal of Tropical Veterinary and Biomedical Research Vol 8, No 2 (2023): Vol. 8 (2) November 2023
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v8i2.36616

Abstract

Herbal antioxidants have gained attention recently as possible treatments for disorders of male reproductive function linked to oxidative stress because of their ability to neutralize free radicals.The investigation into herbal flavonoids for preserving oxidative stress-impaired testicular physiology has been effectively conducted through the visualization of bibliometric analysis. Bibliometric data of published papers collected from the PubMed and Scopus databases in 2018-2023. Network visualization was performed using VOSviewer software on the set keywords that occur (n=15). The literature review was conducted according to the research trends identified by the clusters that appeared in the network visualization. The result revealed that a total of 266 records have been published based on the specified search set keywords since 2018. Through the analysis of keywords, inclusion and exclusion criteria, and the relevant content of papers, 11 articles were identified as suitable for conducting a literature review. Network visualization revealed that the research falls into two general categories: (1) toxicity to the testicles caused by oxidative stress and (2) prevention of oxidative stress impact on tissue through herbal medicine. Examination of the paper's content revealed that certain antioxidant compounds from plants can stabilize free radicals in the testicles. These antioxidants include vitexin, diosmin, hesperidin, phenolic acids, naphthoquinones, fisetin, pachypodol, sciadopitysin, carvacrol, and morin. However, further research is needed to compare active antioxidant content in different herbal plants and identify optimal concentrations for therapeutic effects.
Co-Authors A Sakir, A Sakir A, Aulanniam Abdullah Hamzah, Abdullah Afifuddin Afifuddin, Afifuddin Amalia Sutriana Amiruddin Amiruddin Arifin, Tazul Arman Sayuti Aulia, Usma Azhar A, Azhar Basuki B. Purnomo budianto panjaitan Cut Dahlia Iskandar Cut Nila Thasmi, Cut Nila Darwanis Darwanis Dasrul Dasrul Dwinna Aliza Erdiansyah Rahmi Fadhil, Rahmat Fadillah, Nurul Aini Faisal Faisal Faisal Mustafa Fajar Fakri Fajri, Fauzan Fathurrahman, Fadhil Fitrah Khairi Gani, Fadli A Gani, Fadli A. Gholib G, Gholib Gholib Gholib, Gholib Hafizuddin Hafizuddin Hafizuddin, H Halim, Hendra Hamny H, Hamny Hamny Hamny Hamny Sofyan I. Ketut Mudite Adnyane, I. Ketut Mudite Joharsyah J, Joharsyah Juli Melia Kesuma, T. Meldi Ketut Adnyane Mudite M. Aris Widodo M. Shabri Abd. Majid Mahdi Abrar Masda Admi Mayuri, Herdina Muhammad Bahi Muhammad Hambal Muhammad Hasan Muhammad Isa Muhammad Jalaluddin, Muhammad Muslim Akmal N. Nazaruddin Nanda Yulian Syah Nellita Meutia, Nellita Nirmala, Fadya Nora Usrina Nuzul Asmilia Okta Hilda Kadar, Okta Hilda R Roslizawaty, R Ramadhana, Cut Erika Razali Daud Ridwan Nurdin Rinidar R, Rinidar Riski Ananda, Riski Ritonga, Mudhita Zikkrullah Roslizawaty r Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Sari, Meutia Dwi Novita Siregar, M. Ridha Sitepu, Novi Indriyani Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Suriadi S, Suriadi Suryani, Elfi Satria Sutiman B. Sumitro Syafruddin s Syafruddin Syafruddin Syafruddin, S Syahrizal, Teuku Muhammad T. Armansyah Taufan Hidayat Taufiq Purna Nugraha, Taufiq Purna Tongku Nizwan Siregar Triva Murtina Lubis Umuri, Khairil Zainuddin Z Zainuddin, Zainuddin Zamzami, Rumi Sahara Zulfitri Zulfitri, Zulfitri Zulkifli, Baidillah