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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Co-Authors A Zulaiha Abidin, Mohamad Hamdi Zainal Achmad Bagus Dhani Adi, Mika Adventus Panda Agung Samudra Agustina Tamba, Tio Pinta Ahmad Muammar Kadafi Ajeng Aprilia Alfanaar, Rockiy Alfanaar, Rokiy Alidoust, Marzieh Amalia, RA. Hoetari Tirta Amallia, Ra Hoetari Tirta Amallia, RA Hoetary Tirta Amelia Amilda . Amilda Amilda Amrulloh, Hanif Andi Saputra Anggoro, Rahayu Opi Anita Restu Puji Raharjeng Annissa, Annissa Ansori Rahman Ardy, Adim Al Arif Yachya Arif Yachya Aritonang, Celvin Micael Arsana, Mu afa Arsana, Mu’afa Purwa Arsana, Mu’afa Purwa Ayu, Regina Wahyudyah Sonata Bagus Priambodo Citrariana, Shesanthi Citrariana, Shesanti Dawani Decenly Defilia Anogra Riani Desindria, Galang Rizdho Devana Putri, Salsabila Dewi, Apriana Dhani, Achmad Bagus Dirgantara , Made Dita Monita Dwi Nur Aji, Fajar eka agustina Ekiq Nouval Zaki Elon Jipasca Esther Uwidia Ikhuoria Fadhila Aziz Fandi Tuju Fauzan Fikri Febri Nur Ngazizah Febri Nur Ngazizah Feliyanti Feliyanti Firmansyah, Richo Gerung, Jenny Blessia Gholipour Fereidouni, Hassan Gizem Özbey, Beste Gunawan Gunawan, Yohanes Edy Hapin Afriyani Haq, Muhammad Hasanul Helen Monica Sari Herniadi, Gustam Hery Widijanto I Nyoman, Sudyana, Ike Apriani, Ike Ikechukwu Dorris Chikaodili Ikhazuagbe Hilary Ifijen Indah Wigati Inka Kandida Ira Kendi Irmawan, Muhammad Istiroha Istiroha Ita Erebho Uwidia Jipasca, Elon Kamison Karyadi Karyadi Karyadi Karyadi Kazemkhah Hasankiadeh, Zeynab Kioumarsi, Hamed Kristianto, Sonny Kurratul aini Laode Muh Asdiq Hamsin Ramadan Lestari, Aneke Lumbantobing, Agyl Afryan M Davani, Shabnam Made Dirgantara Magdalin Ulaan Mariasa, Joice Cleriska Marnitha Mayang Tari Monita, Dita Moradi, Arman Muhamad Taufik Hidayat Muhammad Rifqi Shodri Muhammad Rizki Muhammad Sadam Rahmansyah Munir Munir Mu’afa Purwa Arsana Mu’afa Purwa Arsana Ngazizah, Febri Nur Norhusna, Norhusna Novin Teristiandi Nur Hidayat Nurseha, Tito Onny Indriani Pahmedani, Marzieh Alidoust Parametta, Vidya Perdana, Andi Permana, Danafia Permata, Ending Putri Wineni Putri, Nabela Arinda Ra Hoetari Tirta Amallia Ra Hoetari Tirta Amelia Ra Hoetary Tirta Amellia Rachel Obhade Okojie Rafiei, Bahareh Rahman, Ansori Rahman, Sudarman Ramadhan, Riska Retnaningrum, Endah Ria Windi Lestari Riana, Shesanti Citra Riani, Defilia Riri Novita Riri Novita Sunarti Riri Novita Sunarti Riska Ramadhan Ristanto, Yuri Rivai, Sitty Nurqomariah Rr. Mini Sari Wulan Rukmini Rury Eryna Putri Safira, Dwi Septiana Sahari, Yudhi Ekakristian Sandi Mahesa Yudhantara Sari, Helen Monica Sarsozo, Makario Serang, Yitro Shariman Yahaya, Zary Shesanti Citra Riana Shesanti Citra Riana Shodri, Muhammad Rifqi Simajuntak, Ruth Riama Siti Sunariyati Soleha, Siti Sonia Adiba Sukendar, Windu Sulistia, Tiya Sulistyowati Sunarti, Riri Novita Suparman Supriyani, Erni Syarifah Syarifah Syarifah Syarifah, Syarifah Sylvia Monalisa, Shinta Tahrir , Muhammad Thathit Suprayogi Tito Nurseha Tri Oktarina Wasinton Simanjuntak Wijaya, Nuriman Wilda, Robiatul Witari Yahya Febrianto Yanuar Adiba, Faisal Yessy Velina Yithro Serang Yohanes Edi Gunawan Yosaeunike, Vitatani Yudhi Ekakristian Sahari Yuli Nugroho, Yuli Yulita, Elia Zainal Berlian