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Antibacterial Activity Of Freshwater Lobster Shells (Cherax quadricarinatus) Against Escherichia coli Hafizha, Putri; Ridwanto, Ridwanto; Daulay, Anny Sartika; Nasution, Haris Munandar
Indonesian Journal of Science and Pharmacy Vol. 1 No. 3 (2024): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i3.42

Abstract

People still do not utilize freshwater lobster (Cherax quadricarinatus) shells optimally, even though the chitin compound contained in this waste can be modified into chitosan through chemical reactions. Chitosan obtained from freshwater lobster shells can be used as an antibacterial. This study aims to test the antibacterial activity of chitosan from freshwater lobster shell waste against Escherichia coli bacteria. Making chitosan is carried out through three processes, namely, demineralization, deproteination, and deacetylation stages. Then, characterization of the chitosan is carried out, which includes water content, ash content, yield, and solubility. The research results showed that the chitosan obtained in this study was 43.96%, and the degree of deacetylation value of freshwater lobster shell chitosan was 75.11%. Chitosan with a concentration of 0.3, 0.5, 0.7, and 0.9% provides an inhibition zone of 13.5, 15.9, 16.8, and 17.2 mm, respectively, against Escherichia coli. Thus, a concentration of 0.9% chitosan from freshwater lobster shells has better antibacterial activity and is categorized as having strong inhibitory power.
Isolation and Characterization of Chitosan from Kepah Shell Waste (Polymesoda erosa) Percut Sei Tuan, North Sumatera Sitorus, Dara Indah; Ridwanto, Ridwanto
Indonesian Journal of Science and Pharmacy Vol. 1 No. 3 (2024): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i3.43

Abstract

Kepah clams (Polymesoda erosa) are one of the few aquatic animals that have a body and are protected by two cupped shells. Many people consume this shellfish meat, but many of the shells are thrown away by local people so that they only become waste. It is this shell waste that needs to be processed properly because it is known that shells are one of the fishery ingredients that contain chitin. One way to make kepah clams have a higher economic value is by isolating kepah clams into chitosan. The purpose of this study was to isolate and characterize chitosan from mussel shells and to determine functional groups using Fourier infrared (FTIR). The isolation methods used were deproteination, demineralization, depigmentation, deacetylation, and chitosan characterization. The results showed that the yield of the transformation of chitin into chitosan in cockles was 85.77% and had a brownish-white powder texture and no odor. The water content in the chitosan of the clam shells obtained was 1.53%, the ash content was 0.50%, and the degree of deacetylation obtained was 75%.
Antibacterial Activity Test of Ethanol, N-Hexane, and Ethyl Acetate Extracts of Lemon Peel (Citrus x limon (L.) Burm. f.) Nisa, Uswatun; Mambang, D Elysa Putri; Yuniarti, Rafita; Ridwanto, Ridwanto
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.88

Abstract

Staphylococcus hominis and Pseudomonas aeruginosa bacteria are normal flora bacteria found on the surface of human skin, and in certain circumstances can cause infections in the body of a person with a weak immune system. Infection of both bacteria can cause body odor that can affect a person's social relationships. The use of natural antibacterials has minimal side effects, namely lemon peel which is waste from lemons. Lemon juice is commonly used by the public, but not many people know that lemon peel has great benefits like lemon juice. The objective of the research was to determine the chemical compounds contained in the n-hexane fraction, ethyl acetate of lemon peel and antibacterial activity in ethanol extract, n-hexane fraction, ethyl acetate of lemon peel (Citrus x limon (L.) Burm. f.) This research method was an experimental method that includes sample collection and processing, characterization of simple drugs, preparation of 96% ethanol extract, n-hexane fraction, ethyl acetate fraction, phytochemical screening, and antibacterial activity test using the agar diffusion method at concentrations of 10%, 20%, 30%, 40%, 50%. The results of phytochemical screening in the ethyl acetate fraction contain alkaloids, flavonoids, tannins, triterpenoids, and in the n-hexane fraction shows the presence of triterpenoid chemical compounds. Ethanol extract, n-hexane fraction and ethyl acetate fraction of lemon peel have antibacterial activity on Staphylococcus hominis and Pseudomonas aeruginosa bacteria. The results of the three samples tested for the greatest inhibitory power in the ethyl acetate fraction showed intermediate results, almost comparable to the inhibitory power of the positive control ciprofloxacin.
Antibacterial Activity Test of Ethanol Extract, n-Hexane and Ethyl Acetate Fractions of Cardamom Fruit (Amomum cardamomum Soland. Ex Maton Benth) Nada, Dina Qatrun; Mambang, D Elysa Putri; Nasution, M. Amin; Ridwanto, Ridwanto
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.96

Abstract

Bacterial infection is a global health problem that requires special attention, especially due to increasing resistance to antibiotics. This study aims to determine the antibacterial activity of ethanol extract, n-hexane fraction and ethyl acetate fraction of cardamom fruit (Amomum cardamomum Soland. Ex Maton Benth) against Streptococcus pyogenes and Pseudomonas aeruginosa bacteria. The extraction was done by percolation using 96% ethanol, followed by fractionation using n-hexane and ethyl acetate solvents. Phytochemical screening tests showed the presence of alkaloid, flavonoid, tannin, and saponin compounds known to have antibacterial activity. Antibacterial activity test was conducted using disc diffusion method with five concentrations (10%-50%). The results showed that the ethyl acetate fraction had the greatest inhibition, with the highest inhibition zone against Streptococcus pyogenes of 14.1 mm and Pseudomonas aeruginosa of 16.8 mm at 50% concentration. Inhibition increased with increasing concentration. The ethyl acetate fraction proved to be the most effective because it contains semipolar compounds that are able to penetrate the bacterial cell wall. This study supports the potential of cardamom fruit as an alternative natural antibacterial agent.
Penyuluhan Pembuatan Sabun Cuci Tangan dari Daun Jambu Biji Puteri, Cut Intan Annisa; Ningtias, Anggitha; Rani, Zulmai; Dalimunthe, Gabena Indrayani; Nasution, Haris Munandar; Ridwanto, Ridwanto
Jurnal Bakti Nusantara Vol. 1 No. 3 (2024): Jurnal Bakti Nusantara
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/jbn.v1i3.45

Abstract

Sabun cuci tangan merupakan sabun khusus yang dapat membersihkan tangan dari kuman dan debu serta menjaga kulit agar tetap halus dan lembut. Pasca pandemik Covid-19 membuat penggunaan sabun cuci tangan semakin meningkat. Peningkatan ini terjadi karena adaptasi kebiasaan baru untuk selalu mencuci tangan sebelum dan setelah beraktivitas. Tujuan penyuluhan ini adalah agar masyarakat memahami bahwa mencuci tangan sangat penting untuk mencegah masuknya kuman, virus dan bakteri melalui mulut, hidung dan mata. Seiring berjalannya waktu, inovasi tentang tanaman herbal semakin berkembang dan telah banyak penelitian antibakteri yang diteliti. Salah satu tanaman yang terbukti dapat menghambat pertumbuhan bakteri adalah daun jambu biji. Metode yang digunakan dalam kegiatan ini adalah dengan memberikan penyuluhan berupa ceramah interaktif terkait bagaimana cara pembuatan sediaan sabun cuci tangan dari daun jambu biji. Kegiatan pengabdian dilaksanakan di Apotek Farmasi UMN Al-Washliyah. Hasil pengabdian ini diperoleh masyarakat merespon dengan cukup antusias dan merasa penuh syukur memperoleh tambahan pengetahuan terkait pembuatan sabun cuci tangan cair dari daun jambu biji.
Uji Aktivitas Antibakteri Fraksinasi Alkaloid Ekstrak Kulit Kayu Raru (Cotylelobium melanoxylon Pierre) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus Salwa, Shakhila; Daulay , Anny Sartika; Rahayu , Yayuk Putri; Ridwanto, Ridwanto
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 3 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i3.951

Abstract

Background: Indonesia is rich in medicinal plants, including Cotylelobium melanoxylon Pierre (raru bark), traditionally used to treat diarrhea, malaria, and diabetes. However, scientific validation of its alkaloid compounds and antibacterial potential is still limited. Objective: This study aimed to (1) fractionate alkaloids from raru bark extract using chloroform liquid-liquid extraction, (2) identify alkaloid functional groups via FT-IR spectroscopy, (3) quantify alkaloid levels in ethanol and methanol extracts using UV spectrophotometry, and (4) evaluate their antibacterial activity against Escherichia coli and Staphylococcus aureus. Methods: The research stages included plant material processing, ethanol/methanol extraction, phytochemical screening, alkaloid fractionation with chloroform, FT-IR analysis, UV spectrophotometry for alkaloid quantification, and antibacterial testing via disc diffusion at 40%, 50%, and 60% concentrations. Results: Alkaloid fractions were successfully isolated, containing functional groups N-H, C-N, C=O, aromatic C=C, and sharp C=O. The methanol extract yielded higher alkaloid levels (21.03 ± 0.10%) than ethanol (18.95 ± 0.09%). The 50% alkaloid fraction showed the strongest antibacterial activity, with inhibition zones of 21.1 mm (E. coli) and 23.1 mm (S. aureus). Conclusion: Raru bark alkaloids exhibit significant antibacterial effects, with methanol extract demonstrating superior alkaloid content and efficacy.
Aktivitas Antijamur Kitosan Dari Cangkang Kerang Bambu (Solen corneus) Pada Pembuatan Hidrogel Terhadap Trichophyton mentagrophytes Hasibuan , Nabila Hasim; Ridwanto, Ridwanto; Nasution , Haris Munandar; Rani , Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 3 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i3.906

Abstract

Background: Hydrogels are topical preparations applied to the skin surface. Chitosan, a compound derived from the deacetylation of chitin, has various benefits, including antifungal activity. Utilizing waste from bamboo clam shells (Solen courneus) collected in Belawan waters as a source of chitosan could provide a sustainable solution for developing antifungal hydrogel formulations. Objective: This study aimed to produce chitosan from bamboo clam shells, formulate it into a stable hydrogel, and evaluate its antifungal activity against Trichophyton mentagrophytes. Methods: This experimental study involved isolating chitosan through deproteinization, demineralization, depigmentation, and deacetylation steps. The resulting chitosan was characterized by moisture content, ash content, yield, solubility, and functional group identification using FTIR spectroscopy. Subsequently, chitosan was formulated into four hydrogel variations (0%, 2%, 4%, and 6%). The hydrogel formulations were evaluated for organoleptic properties, homogeneity, pH, viscosity, spreadability, adhesiveness, and antifungal activity. Results: The produced chitosan exhibited functional groups similar to standard chitosan and a degree of deacetylation of 77.5%. Hydrogel evaluation over three weeks showed good stability, with pH values of 4–6.5, spreadability of 5–7 cm, adhesiveness greater than 1 second, and viscosity ranging from 3,000–50,000 cps, all meeting the required standards. The antifungal test demonstrated that the 6% chitosan hydrogel had the highest inhibition zone (11.5 mm) against Trichophyton mentagrophytes. Conclusion: Chitosan derived from bamboo clam shells was successfully formulated into a stable hydrogel with effective antifungal activity. The 6% concentration exhibited the strongest antifungal effect, indicating its potential as a topical preparation for treating skin fungal infections.
PHENOLIC, FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY WITH SUN PROTECTION FACTOR VALUE OF RED BETEL LEAF ETHANOL EXTRACT (Piper ornatum N. E. Br) Yuniarti, Rafita; Rani, Zulmai; Ridwanto, Ridwanto; Lubis, Minda Sari
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 10, No 2 (2025): September
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30604/jika.v10i2.3149

Abstract

Red betel leaf is a plant that is rich in benefits, one of which is as an antioxidant. The benefits as an antioxidant in plants are due to the content of phenolic and flavonoid compounds. Phenolic compounds also function as protection against UV-B rays, this ability can be seen from the SPF value. The purpose of this study was to determine the amount of phenolic and flavonoid compounds and the antioxidant and sunscreen activity of ethanol extract from red betel leaves. Phenolic content was determined using 10% Follin Ciocalteu reagent and 20% Na2CO3. In contrast, flavonoid content used AlCl3 and CH3COONa solutions, and the determination of antioxidant activity was carried out using DPPH. In contrast, the ability a protect against sunlight (sunscreen) was carried out by determining the sun protection factor (SPF) value. After measurements were carried out using a UV-visible spectrophotometer, the results obtained were a phenolic content of 257.56 mg GAE/g and flavonoids of 46.8008 mg QE/g. They had a very strong antioxidant activity with an IC50 value of 38.9338 ppm and had sunscreen activity with an SPF value of 5, 17, 28, 36, and 38 at concentrations of 100, 300, 500, 700, and 1000 ppm respectively.
Green Synthesis of Copper Nanoparticles Using Syzygium polyanthum Extract and Their Antioxidant Potential via DPPH Assay Nasution, Shamila Sufi Aulia; Miswanda, Dikki; Ridwanto, Ridwanto; Dalimunthe, Gabena Indrayani
Indonesian Journal of Science and Pharmacy Vol. 3 No. 1 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v3i1.117

Abstract

Copper nanoparticles (CuNPs) have gained attention for their cost-effective synthesis and potent antioxidant properties, offering a promising approach to address the bioavailability challenges of herbal medicines. This study investigates the green synthesis of CuNPs using bay leaf (Syzygium polyanthum (Wight.) Walp.) extract as a bioreductor and evaluates their antioxidant activity via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Bay leaf extract, rich in flavonoids and phenolic compounds, was prepared through heating extraction and mixed with copper nitrate (CuNO₃) at varying ratios (1:1, 1:2, 1:3, 1:4). The formation of CuNPs was confirmed by a color change from yellow to greenish-brown after 24 hours, with particle sizes ranging from 527.48 nm to 3256.81 nm, as determined by Particle Size Analysis (PSA). UV-Vis spectrophotometry verified the reduction of Cu⁺ ions, with Cu concentrations decreasing post-synthesis. The antioxidant activity of CuNPs exhibited an IC₅₀ value of 15.49 ppm, classified as very strong, compared to 72.09 ppm for the bay leaf extract alone (strong) and 13.82 ppm for vitamin C (very strong). These results demonstrate that bay leaf extract-mediated CuNPs possess enhanced antioxidant potential, highlighting the efficacy of green synthesis in producing bioactive nanoparticles for potential therapeutic applications.
Analisis Kadar Parasetamol dan Kafein Dalam Sediaan Tablet Menggunakan Metode Spektrofotometri Derivatif Zero Crossing Rosaldi, Hikmah; Pulungan, Ainil Fithri; Ridwanto, Ridwanto
Jambura Journal of Chemistry Vol 7, No 2 (2025): August
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jambchem.v7i2.24392

Abstract

The development of drugs in the form of combination tablets at this time has increased a lot; combining drugs in a tablet preparation can provide an increasing reaction and ability. This makes combining drugs such as Paracetamol and caffeine an effective combination in the pharmaceutical field. Paracetamol is an antipyretic analgesic drug that can be combined with caffeine which can be used in therapy with a combination of these drugs. The objective of this research was to ascertain the concentration of a combination of paracetamol and caffeine in a tablet formulation by employing a zero cross-derivative spectrophotometric method. In this study, solvent optimization was carried out with 17 categories of solvent selection, and acetate: methanol (30:70) was selected using the zero cross-derivative methods with a dA / d plot utilizing the maximum wavelength of paracetamol 258 nm and caffeine 288 nm, because there was an actual wavelength shift in this analysis. To validate the determination of the level of the zero cross-derivative method using the second absorption in the ace solvent (30:70 acetate: methanol). Spectrophotometric analysis of zero crossing derivatives of a mixture of paracetamol and caffeine found that paracetamol levels in tablet A were 95.24 8.47%, in tablet B of 92.86 0%, and in tablet C of 96.5 0%, while the caffeine content was 104.89 0%, 102.09 0%, and 91.6 0%. According to USP 30 NF 25 (2007), it can be concluded that the test results of the composition of paracetamol and caffeine in tablets meet the requirements.
Co-Authors Abdullah, Husaini Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Ainil Fithri Pulungan Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alistraja Dison Silalahi Alkausar Saragih Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Andi Syahputra, Ricky Ani Sutiani Anjarlit Romiyuliana Br Parapat Annisa Fadilah Amir Annisa Utami Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Asep Trizaldi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Bangun Lubis Cici Andriani Claudy Friesta Melanie Dahlia Hasibuan Dahnia Ayu Fitria Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Elma Natasya Endang D. Hamid Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fadya Azzahra Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fathur Rahman Harun Fatin Fairuz Pasaribu Fatur Rahman Fiqri Hermawan Fithri Pulungan, Ainil FRANSISKA, EVA Gabena Indrayani Dalimunthe Hafizha, Putri Hakimi Hakimi Harahap, Ade Try Atwinda Harahap, Siti Salimah Haris Munandar Nasution Haris Munandar Nst Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Ihsan Fadhilah Imelda Natasia Br S Meliala Indra B. Hutagalung Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi M. Saleh Sitompul Maghfirah , Dini Makhfirah Malahayati Malahayati Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Marissa Tasya Adawiyah Maryanti Yuza Maya Syafira Medhy Ardhita Aprilia Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Al Hudawi Muhammad Al Qodri Muhammad Alfariz Baihaqi Siregar Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Wahyuddin Abdullah Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nomi Noviani Nur Ainun Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Nurmazela, Virda Pitaloka, Syifa Amilia Pulungan, Ainil Fitri Puteri, Cut Intan Annisa Putri Intan Sari Putri Khairani Putri Theresia Harianja Putri Yupa Hasibuan Putriyana Putriyana Qisti Pristiwani Rafita Yuniarti Rahayu , Yayuk Putri Rahayu Rahayu Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmayanti, Putri Reza Irma Ricky Andi Syahputra Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Robiatun Rambe Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Sari, Farida Sarinah Hasibuan Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shakila Salwa Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin Siregar , Anisah Siti Rahmasari Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Sri Wahyuni Sumiyarni Harahap Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah, Aulia Tamara Army Tambupolon, Sanya Tanika Utama, Fazrul Amin Vera Estefania Kaban Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani