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Antibacterial Activity of Kaffir Lime Leaf (Citrus hystrix DC) Ethanol Extract against Staphylococcus aureus and Escherichia coli Azhar, Gustika; Ridwanto, Ridwanto; Nasution, Haris Munandar; Pulungan, Ainil Fithri; Rani, Zulmai
Indonesian Journal of Science and Pharmacy Vol. 2 No. 1 (2024): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i1.48

Abstract

The use of herbal medicine has been increasing, which encourages researchers to use herbal materials as an alternative antibacterial, one of which is the kaffir lime leaf plant. This study was to identify the group of secondary metabolite compounds and examine the antibacterial properties of ethanol extract of kaffir lime leaves (Citrus hystrix DC) against Staphylococcus aureus and Escherichia coli bacteria. This study began with the preparation of simplisia powder, extracting kaffir lime leaf with ethanol, and the antibacterial activity test of extracts. The results of the study showed that the secondary metabolites contained in kaffir lime leaves are alkaloids, flavonoids, saponins, tannins, steroids and glycosides. Antibacterial activity test on Staphylococcus aureus bacteria showed that the ethanol extract of kaffir lime leaves with the best concentration inhibited the bacterial inhibition zone at a concentration of 10% with an inhibition diameter of 22.21 mm with a very strong category dan the best concentration is 10% with an inhibition diameter of 20.48 mm with a very strong category for Escherichia coli bacteria. Ethanol extract of Kaffir Lime leaves has been proven to inhibit Staphylococcus aureus and Escherichia coli bacteria.
Determination of Total Flavonoid of Ethanol Extract of Pegagan Leaves (Centella asiatica (L.) Urb) Using Maceration and Soxhletation Methods Wahyudi, Muhammad; Manik, Umi Chairani; Ridwanto, Ridwanto; Daulay, Anny Sartika; Rahmadani, Rahmadani
Indonesian Journal of Science and Pharmacy Vol. 2 No. 1 (2024): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i1.63

Abstract

A study was conducted to determine the total flavonoid content of ethanol extract of pegagan leaves (Centella asiatica (L.) Urb) using maceration and soxhletation methods. The study began with the preparation of pegagan leaf simplicia and its characterization, preparation of ethanol extract by maceration and soxhletation, phytochemical screening and determination of total flavonoid content of ethanol extract of pegagan leaves using visible spectrophotometry method. The results of phytochemical screening of ethanol extract of pegagan leaves indicated the presence of alkaloid, flavonoid, saponin, tannin, steroid/triterpenoid and glycoside compounds. The results of determining the total flavonoid content in ethanol solvent extract by maceration were 31.6416±0.1534 mgQE/g and ethanol solvent extract by soxhletation were 20.832±0.0514 mgQE/g. Extraction of pegagan leaves by maceration method was more effective than soxhletation method.
Toxicity Test Of Ethanol Extract Of Pletekan Leaves (Ruellia tuberosa L) Using The Brine Shrimp Lethality Test (BSLT) Method Fajri, Fajrilda Aqilah; ridwanto, ridwanto; Pulungan, Ainil Fithri; Rani, Zulmai
Indonesian Journal of Science and Pharmacy Vol. 2 No. 1 (2024): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i1.71

Abstract

The pletekan plant (Ruellia tuberosa L.) is a family of Acanthaceae Ruellia tuberosa L.) one of the plants used as a medicinal plant, especially the leaves, have been used empirically for therapy as a diuretic, antidiabetic, antihypertensive. Also used in the treatment of syphilis, kidney stones, cancer, heart disease, colds, hypertension and digestive problems. The purpose of this study was to determine the toxicity by determining the LC50 in the ethanol extract of pletekan leaves (Ruellia tuberosa L). The analysis carried out was a toxicity test on the ethanol extract of pletekan leaves against Artemia Salina Leach shrimp larvae using the BSLT method with various concentrations, namely 10, 15, 20, 25, 30, 35, and 40 ppm and determining the LC50 value with probit analysis. Based on the results of the toxicity test, the LC50 value was 96.4595 µg/Ml. These results show that the ethanol extract of pletekan leaves (Ruellia tuberosa L) has a toxic effect on Artemia Salina Leach shrimp larvae.
PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL DAN EKSTRAK ETIL ASETAT DAUN PALA (Myristica fragrans Houtt) SECARA SPEKTROFOTOMETRI VISIBLE Mirna Mirna; Muhammad Amin Nasution; Ridwanto Ridwanto; Anny Sartika Daulay
FORTE JOURNAL Vol 4 No 1 (2024): Edisi Januari 2024
Publisher : Universitas Haji Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51771/fj.v4i1.762

Abstract

Penggunaan tumbuhan sebagai ramuan obat sangat berkaitan dengan kandungan kimia yang terdapat dalam tumbuhan tersebut terutama zat aktif biologisnya. Senyawa bioaktif yang terdapat dalam tumbuhan merupakan senyawa metabolit sekunder seperti steroid, flavonoid, alkaloid, saponin, terpenoid dan tanin. Salah satu tanaman yang mengandung senyawa metabolit sekunder yaitu daun pala (Myristica fragrans Houtt). Tujuan penelitian ini adalah untuk mengetahui senyawa kimia yang terdapat didalam ekstrak etanol dan ekstrak etil asetat dan untuk mengetahui nilai flavonoid total ekstrak etanol dan ekstrak etil asetat daun pala. Tahapan penelitian ini meliputi pengolahan bahan tumbuhan, pembuatan ekstrak etanol dan ekstrak etil asetat, pemeriksaan karakterisasi, skrining fitokimia dan penetapan kadar flavonoid total ekstrak etanol dan ekstrak etil asetat daun pala dengan metode spektrofotometri Visible. Ekstrak daun pala dibuat dengan metode maserasi dengan menggunakan etanol 70% dan etil asetat ekstrak yang diperoleh dipekatkan dengan rotary evaporator, Selanjutnya dilakukan penetapan kadar flavonoid total dengan metode spektrofotometri Visible. Hasil skrining fitokimia pada ekstrak etanol dan ekstrak etil asetat daun pala terdapat kandungan golongan senyawa kimia seperti alkaloid, flavonoid, saponin, tanin, dan steroid/triterpenoid. Hasil penentuan kadar flavonoid total pada ekstrak etanol sebesar 39,7252 ± 1,9596 mg QE/g dan  ekstrak etil asetat sebesar 31,1395  ± 0,3983 mg QE/g.
PENENTUAN KADAR RHODAMIN B PADA LIPSTIK YANG DIJUAL DI KOTA BANDA ACEH SECARA KROMATOGRAFI LAPIS TIPIS DENSITOMETRI MANUAL Adyani Maulinda; Ridwanto Ridwanto; Anny Sartika Daulay; Haris Munandar Nasution; Zulmai Rani
FORTE JOURNAL Vol 4 No 1 (2024): Edisi Januari 2024
Publisher : Universitas Haji Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51771/fj.v4i1.763

Abstract

Rhodamin B dalam kosmetika tidak diperbolehkan sesuai No.445/MenKes/Per/V/1998. Penggunaan Rhodamin B dapat mengiritasi saluran pernapasan, penumpukannya dapat menyebabkan gangguan fungsi hati. Penelitian ini bertujuan untuk mengetahui adanya kandungan Rhodamin B serta kadar Rhodamin B pada lipstik, untuk mengetahui cara analisis kualitatif dan kuantitatif Rhodamin B pada lipstik secara KLT Densitometri. Penelitian ini meliputi cara pengumpulan dan pembuatan larutan lipstik, pembuatan larutan Rhodamin B, pengujian Rhodamin B dan penetapan kadar Rhodamin B. Penelitian ini menggunakan 5  lipstik. Analisis kualitatif secara KLT menggunakan fase diam silika gel GF254 dan fase gerak etil asetat : metanol : ammonia (15:3:3). Analisis kuantitatif menggunakan metode Densitometri. Hasil analisis kualitatif secara KLT didapat, 2 dari 5 sampel lipstik positif Rhodamin B ditandai dengan noda berwarna orange dan berfluoresensi kuning pada lampu UV 254 nm dan 366 nm, bewarna merah muda secara visual. Nilai Rf lipstik S1 (0,68), Rf lipstik S2 (0,45), Rf lipstik S3 (0,87), Rf lipstik S4 (0,87) dan Rf lipstik S5 (0,56) dengan Rf Rhodamin B (0,87). Lipstik positif Rhodamin B dilakukan penetapan kadar dengan metode Densitometri. Kurva Kalibrasi diperoleh persamaan y = 0,221x + 1,088. Kadar Rhodamin B pada lipstik S3 sebesar 1,505 mg/g dan lipstik S4 sebesar 3,118 mg/g. Kesimpulannya lipstik kode S3 dan S4 tidak memenuhi uji persyaratan.
UJI SITOTOKSISITAS EKSTRAK ETANOL DAUN TAHI AYAM (Tagetes erecta L.) DENGAN METODE BSLT (Brine Shrimp Lethality Test) Putri Khairani; Ridwanto Ridwanto; Anny Sartika Daulay; Haris Munandar Nasution; Zulmai Rani
FORTE JOURNAL Vol 4 No 2 (2024): Edisi Juli 2024
Publisher : Universitas Haji Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51771/fj.v4i2.921

Abstract

Uji sitotoksik adalah pemeriksaan yang menentukan tingkat kerusakan zat terhadap sel. Penyakit yang dikenal sebagai kanker dicirikan oleh pembelahan sel yang tidak terkendali dan kemampuan sel-sel tersebut untuk menyerang jaringan biologis lainnya. Senyawa aktif tanaman herbal dapat berupa ekstrak tanaman atau ekstrak tanaman. Daun tahi ayam adalah salah satu tumbuhan yang mengandung banyak manfaat. Untuk mengetahui apakah larva udang Artemia salina Leach memiliki keidentikan dengan sel kanker manusia, uji pendahuluan dilakukan dengan metode BSLT.Penelitian ini bertujuan untuk mengetahui golongan senyawa metabolit sekunder yang memiliki kemampuan efek sitotoksik pada ekstrak etanol daun tahi ayam. Dalam penelitian ini, ekstrak etanol daun tahi ayam dikarakterisasi, di skrining fitokimia, dan diuji untuk sitotoksisitas dengan menggunakan metode BSLT terhadap larva udang Artemia salina Leach, yang ditunjukkan dengan nilai LC50. Dalam berbagai konsentrasi, seperti 100, 200, 300, 400, 500, 600, 700, 800, 900, dan 1000 ppm, uji sitotoksisitas dilakukan. Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun tahi ayam mengandung senyawa metabolit sekunder seperti alkaloid, flavonoid, tanin, saponin, dan steroid, dan uji sitotoksisitas dengan probit menunjukkan nilai LC50 319,6686 g/ml. Oleh karena itu, penelitian ini menunjukkan bahwa ekstrak etanol daun tahi ayam bersifat toksik dan mungkin berfungsi sebagai antikanker.
Determination of Total Flavonoid Content of Raru Bark Extract: Cotylelobium Melanoxylon Pierre at Various Methanol Concentrations and Antibacterial Activity Against Staphylococcus Hazizah, Mautia; Ridwanto, Ridwanto; Daulay, Ani Sartika
Journal La Medihealtico Vol. 6 No. 2 (2025): Journal La Medihealtico
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallamedihealtico.v6i2.1949

Abstract

Raru bark (Cotylelobium melanoxylon (Hook.f) Pierre) is a plant with antidiabetic properties. Raru bark plants contain flavonoids, saponins, and tannins. The purpose of this study was to determine the class of secondary metabolites contained in the sample, determine flavonoid levels, and determine antibacterial activity against Staphylococcus aureus bacteria. This research includes processing samples made simplisia, then extracted and thickened using rotary evaporatory. Simplisia was tested for phytochemical screening to determine the content of metabolite compounds, in addition to testing its characteristics. The next test was the determination of total flavonoid content using UV-Vis spectrophotometry. Followed by testing antibacterial activity against Staphylococcus aureus. The results of phytochemical screening obtained flavonoids, tannins, and saponins. Flavonoid levels were 42.4308 ± 2.4739 mgQE/g; 38.1813 ± 1.5799mgQE/g; and 23.8153 ± 3.049276 mgQE/g, respectively. In testing antibacterial activity using concentrations of 10%, 40%, 70%, and 100%, and the highest concentration is found at a concentration of 100% which is 19.83mm.
Detection and Quantification of Rhodamine B in Loose Powder and Blush Cosmetic Products Munthe, Herlina; Sartika Daulay, Anny; Ridwanto, Ridwanto; Munandar Nasution, Haris
Indonesian Journal of Pharmaceutical Education Vol 5, No 2 (2025): May–August 2025
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v5i2.30832

Abstract

The use of decorative cosmetics such as loose powder and blush is increasingly widespread, raising concerns regarding the safety of the coloring agents used, particularly Rhodamine B. This study aimed to identify the presence and determine the levels of Rhodamine B in loose powder and blush cosmetic products using Thin Layer Chromatography (TLC) for qualitative analysis and High-Performance Liquid Chromatography (HPLC) for quantitative analysis. The HPLC method applied in this study was validated and showed good linearity, precision, and sensitivity in accordance with ICH guidelines. A total of 16 cosmetic samples were analyzed, and 3 samples were confirmed to contain Rhodamine B. The highest Rhodamine B level was found in sample X at 66.54 µg/g, followed by sample Z at 21.03 µg/g, and sample Y at 0.83 µg/g. These findings indicate that the presence of Rhodamine B in cosmetic products remains a concern. Therefore, it is necessary to strengthen regulatory supervision and increase public awareness to select safe cosmetic products that are officially registered by BPOM.
Determination of Total Flavonoid Levels of Bajakah Wood Extract (Spatholobus Littoralis Hassk) on Various Ethanol Concentrations and Antibacterial Activity Test of Staphylococcus Aureus Rahmasari, Siti; Ridwanto, Ridwanto; daulay, Anny sartika; Nst, Haris Munandar
Eduvest - Journal of Universal Studies Vol. 5 No. 3 (2025): Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v5i3.50927

Abstract

The bajakah plant is one of the biological plants used in traditional medicine. Bajakah is also a medicinal herbal plant that can be used in all its parts. This plant can be found in the depths of Kalimantan Province, but it has not spread to other regions. There has been no effort to cultivate bajakah because of the lack of understanding of the local population about the use of this plant. This research includes the stages of processing plant materials, making ethanol extracts, characterization examinations, phytochemical screening, and determining total flavonoid levels of ethanol extract of Bajakah wood using the visible spectrophotometry method and antibacterial activity. Bajakah wood extract was made by maceration method using ethanol concentrations of 96%, 70%, and 50%, and the extract obtained was concentrated with a rotary evaporator, then the total flavonoid level was determined using the visible spectrophotometry method and antibacterial activity tests were carried out against Staphylococcus aureus As a positive control using chloramphenicol and negative control using dimethyl sulfoxide. The results were obtained by determining the maximum wavelength of quercetin and operating time, measuring the quercetin calibration curve, and calculating the total flavonoid level using the visible spectrophotometry method. The results obtained in the determination of total flavonoid levels in Bajakah wood ethanol extract at a concentration of 96% were 349.888 ± 4.5022 mg QE/g, at a 70% concentration of 307.137 ± 6.7626 mg QE/g and a 50% concentration of 6.15173 ± 2.9148 mg QE/g.
Formulasi dan Uji SPF Sunscreen Powder dari Nanopartikel Ekstrak Kulit Jeruk Bali (Citrus maxima (Burm) Merr.): Formulation and SPF Testing of Sunscreen Powder from Nanoparticle Extract of Pomelo Peel (Citrus maxima (Burm) Merr.) Azri, Atika; Ridwanto, Ridwanto; Daulay, Anny SArtika; Dalimunthe, Gabena Indriyani
Jurnal Surya Medika (JSM) Vol. 11 No. 1 (2025): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v11i2.9256

Abstract

Radiasi ultraviolet (UV) merupakan salah satu ancaman utama bagi kesehatan kulit yang berkontribusi pada terjadinya penuaan dini, kerusakan DNA, dan peningkatan risiko kanker kulit. Senyawa antioksidan, seperti flavonoid yang banyak ditemukan pada tumbuhan, memiliki kemampuan untuk menetralkan radikal bebas yang dihasilkan akibat paparan sinar UV. Penelitian ini bertujuan untuk menghasilkan potensi ekstrak kulit jeruk bali, yang kaya akan flavonoid, sebagai tabir surya alami. Untuk meningkatkan stabilitas dan homogenitas formulasi, ekstrak kulit jeruk bali dimodifikasi menjadi nanopartikel, yang diharapkan dapat meningkatkan efektivitasnya dalam sediaan tabir surya bubuk. Formulasi sediaan dilakukan dalam empat konsentrasi, yaitu formulasi blanko, F1 (5%), F2 (10%), dan F3 (15%). Evaluasi mutu fisik sediaan meliputi uji organoleptis, homogenitas, derajat halus, daya lekat, derajat keasaman (pH), hedonik/kesukaan, dan Sun Protection Factor (SPF). Hasil penelitian menunjukkan bahwa uji mutu fisik sediaan tabir surya bubuk berbasis ekstrak nanopartikel kulit jeruk bali memenuhi kriteria yang ditetapkan. Ekstrak nanopartikel yang dihasilkan memiliki ukuran 205,2 nm, indeks polidispersitas 0,359, dan zeta potensial -16,0 mV. Nilai SPF dari formulasi tabir surya bubuk berturut-turut adalah 1,617 (minimal), 7,946 (ekstra), 16,554 (ultra), dan 19,072 (ultra), menunjukkan efektivitas perlindungan yang signifikan terhadap sinar UV. Penelitian ini memberikan pengetahuan baru mengenai potensi penggunaan ekstrak tumbuhan sebagai alternatif dalam pengembangan produk tabir surya alami.
Co-Authors Abdullah, Husaini Abdullah, Muhammad Wahyuddin Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Andi Syahputra, Ricky Ani Sutiani Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Cici Andriani Claudy Friesta Melanie Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Dison Silalahi, Alistraja Elma Natasya Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fatur Rahman Fithri Pulungan, Ainil FRANSISKA, EVA Gabena Indrayani Dalimunthe Hafizha, Putri Harahap, Ade Try Atwinda Harahap, Siti Salimah Harahap, Sumiyarni Haris Munandar Nasution Harun , Fathur Rahman Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Imelda Natasia Br S Meliala Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi Maghfirah , Dini Makhfirah Malahayati Malahayati Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Maryanti Yuza Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nst, Haris Munandar Nur Ainun Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Nurmazela, Virda Parapat , Anjarlit Romiyuliana Br Pitaloka, Syifa Amilia Pulungan, Ainil Fitri Puteri, Cut Intan Annisa Putri Khairani Putri Theresia Harianja Putriyana Putriyana Qisti Pristiwani Rahayu , Yayuk Putri Rahayu Rahayu Rahma Dona, Rahma Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmayanti, Putri Rambe, Robiatun Reza Irma Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Saragih, Alkausar Sari, Farida Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin, Siradjuddin Siregar , Anisah Siregar, Muhammad Alfariz Baihaqi Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah Aulia Tamara Army Tambupolon, Sanya Tanika Trizaldi, Asep Utama, Fazrul Amin Vera Estefania Kaban Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani