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PENENTUAN KADAR RHODAMIN B PADA LIPSTIK YANG DIJUAL DI KOTA BANDA ACEH SECARA KROMATOGRAFI LAPIS TIPIS DENSITOMETRI MANUAL Adyani Maulinda; Ridwanto Ridwanto; Anny Sartika Daulay; Haris Munandar Nasution; Zulmai Rani
FORTE JOURNAL Vol 4 No 1 (2024): Edisi Januari 2024
Publisher : Universitas Haji Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51771/fj.v4i1.763

Abstract

Rhodamin B dalam kosmetika tidak diperbolehkan sesuai No.445/MenKes/Per/V/1998. Penggunaan Rhodamin B dapat mengiritasi saluran pernapasan, penumpukannya dapat menyebabkan gangguan fungsi hati. Penelitian ini bertujuan untuk mengetahui adanya kandungan Rhodamin B serta kadar Rhodamin B pada lipstik, untuk mengetahui cara analisis kualitatif dan kuantitatif Rhodamin B pada lipstik secara KLT Densitometri. Penelitian ini meliputi cara pengumpulan dan pembuatan larutan lipstik, pembuatan larutan Rhodamin B, pengujian Rhodamin B dan penetapan kadar Rhodamin B. Penelitian ini menggunakan 5  lipstik. Analisis kualitatif secara KLT menggunakan fase diam silika gel GF254 dan fase gerak etil asetat : metanol : ammonia (15:3:3). Analisis kuantitatif menggunakan metode Densitometri. Hasil analisis kualitatif secara KLT didapat, 2 dari 5 sampel lipstik positif Rhodamin B ditandai dengan noda berwarna orange dan berfluoresensi kuning pada lampu UV 254 nm dan 366 nm, bewarna merah muda secara visual. Nilai Rf lipstik S1 (0,68), Rf lipstik S2 (0,45), Rf lipstik S3 (0,87), Rf lipstik S4 (0,87) dan Rf lipstik S5 (0,56) dengan Rf Rhodamin B (0,87). Lipstik positif Rhodamin B dilakukan penetapan kadar dengan metode Densitometri. Kurva Kalibrasi diperoleh persamaan y = 0,221x + 1,088. Kadar Rhodamin B pada lipstik S3 sebesar 1,505 mg/g dan lipstik S4 sebesar 3,118 mg/g. Kesimpulannya lipstik kode S3 dan S4 tidak memenuhi uji persyaratan.
UJI SITOTOKSISITAS EKSTRAK ETANOL DAUN TAHI AYAM (Tagetes erecta L.) DENGAN METODE BSLT (Brine Shrimp Lethality Test) Putri Khairani; Ridwanto Ridwanto; Anny Sartika Daulay; Haris Munandar Nasution; Zulmai Rani
FORTE JOURNAL Vol 4 No 2 (2024): Edisi Juli 2024
Publisher : Universitas Haji Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51771/fj.v4i2.921

Abstract

Uji sitotoksik adalah pemeriksaan yang menentukan tingkat kerusakan zat terhadap sel. Penyakit yang dikenal sebagai kanker dicirikan oleh pembelahan sel yang tidak terkendali dan kemampuan sel-sel tersebut untuk menyerang jaringan biologis lainnya. Senyawa aktif tanaman herbal dapat berupa ekstrak tanaman atau ekstrak tanaman. Daun tahi ayam adalah salah satu tumbuhan yang mengandung banyak manfaat. Untuk mengetahui apakah larva udang Artemia salina Leach memiliki keidentikan dengan sel kanker manusia, uji pendahuluan dilakukan dengan metode BSLT.Penelitian ini bertujuan untuk mengetahui golongan senyawa metabolit sekunder yang memiliki kemampuan efek sitotoksik pada ekstrak etanol daun tahi ayam. Dalam penelitian ini, ekstrak etanol daun tahi ayam dikarakterisasi, di skrining fitokimia, dan diuji untuk sitotoksisitas dengan menggunakan metode BSLT terhadap larva udang Artemia salina Leach, yang ditunjukkan dengan nilai LC50. Dalam berbagai konsentrasi, seperti 100, 200, 300, 400, 500, 600, 700, 800, 900, dan 1000 ppm, uji sitotoksisitas dilakukan. Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun tahi ayam mengandung senyawa metabolit sekunder seperti alkaloid, flavonoid, tanin, saponin, dan steroid, dan uji sitotoksisitas dengan probit menunjukkan nilai LC50 319,6686 g/ml. Oleh karena itu, penelitian ini menunjukkan bahwa ekstrak etanol daun tahi ayam bersifat toksik dan mungkin berfungsi sebagai antikanker.
Determination of Total Flavonoid Content of Raru Bark Extract: Cotylelobium Melanoxylon Pierre at Various Methanol Concentrations and Antibacterial Activity Against Staphylococcus Hazizah, Mautia; Ridwanto, Ridwanto; Daulay, Ani Sartika
Journal La Medihealtico Vol. 6 No. 2 (2025): Journal La Medihealtico
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallamedihealtico.v6i2.1949

Abstract

Raru bark (Cotylelobium melanoxylon (Hook.f) Pierre) is a plant with antidiabetic properties. Raru bark plants contain flavonoids, saponins, and tannins. The purpose of this study was to determine the class of secondary metabolites contained in the sample, determine flavonoid levels, and determine antibacterial activity against Staphylococcus aureus bacteria. This research includes processing samples made simplisia, then extracted and thickened using rotary evaporatory. Simplisia was tested for phytochemical screening to determine the content of metabolite compounds, in addition to testing its characteristics. The next test was the determination of total flavonoid content using UV-Vis spectrophotometry. Followed by testing antibacterial activity against Staphylococcus aureus. The results of phytochemical screening obtained flavonoids, tannins, and saponins. Flavonoid levels were 42.4308 ± 2.4739 mgQE/g; 38.1813 ± 1.5799mgQE/g; and 23.8153 ± 3.049276 mgQE/g, respectively. In testing antibacterial activity using concentrations of 10%, 40%, 70%, and 100%, and the highest concentration is found at a concentration of 100% which is 19.83mm.
Detection and Quantification of Rhodamine B in Loose Powder and Blush Cosmetic Products Munthe, Herlina; Sartika Daulay, Anny; Ridwanto, Ridwanto; Munandar Nasution, Haris
Indonesian Journal of Pharmaceutical Education Vol 5, No 2 (2025): May–August 2025
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v5i2.30832

Abstract

The use of decorative cosmetics such as loose powder and blush is increasingly widespread, raising concerns regarding the safety of the coloring agents used, particularly Rhodamine B. This study aimed to identify the presence and determine the levels of Rhodamine B in loose powder and blush cosmetic products using Thin Layer Chromatography (TLC) for qualitative analysis and High-Performance Liquid Chromatography (HPLC) for quantitative analysis. The HPLC method applied in this study was validated and showed good linearity, precision, and sensitivity in accordance with ICH guidelines. A total of 16 cosmetic samples were analyzed, and 3 samples were confirmed to contain Rhodamine B. The highest Rhodamine B level was found in sample X at 66.54 µg/g, followed by sample Z at 21.03 µg/g, and sample Y at 0.83 µg/g. These findings indicate that the presence of Rhodamine B in cosmetic products remains a concern. Therefore, it is necessary to strengthen regulatory supervision and increase public awareness to select safe cosmetic products that are officially registered by BPOM.
Formulasi dan Uji SPF Sunscreen Powder dari Nanopartikel Ekstrak Kulit Jeruk Bali (Citrus maxima (Burm) Merr.): Formulation and SPF Testing of Sunscreen Powder from Nanoparticle Extract of Pomelo Peel (Citrus maxima (Burm) Merr.) Azri, Atika; Ridwanto, Ridwanto; Daulay, Anny SArtika; Dalimunthe, Gabena Indriyani
Jurnal Surya Medika (JSM) Vol. 11 No. 1 (2025): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v11i2.9256

Abstract

Radiasi ultraviolet (UV) merupakan salah satu ancaman utama bagi kesehatan kulit yang berkontribusi pada terjadinya penuaan dini, kerusakan DNA, dan peningkatan risiko kanker kulit. Senyawa antioksidan, seperti flavonoid yang banyak ditemukan pada tumbuhan, memiliki kemampuan untuk menetralkan radikal bebas yang dihasilkan akibat paparan sinar UV. Penelitian ini bertujuan untuk menghasilkan potensi ekstrak kulit jeruk bali, yang kaya akan flavonoid, sebagai tabir surya alami. Untuk meningkatkan stabilitas dan homogenitas formulasi, ekstrak kulit jeruk bali dimodifikasi menjadi nanopartikel, yang diharapkan dapat meningkatkan efektivitasnya dalam sediaan tabir surya bubuk. Formulasi sediaan dilakukan dalam empat konsentrasi, yaitu formulasi blanko, F1 (5%), F2 (10%), dan F3 (15%). Evaluasi mutu fisik sediaan meliputi uji organoleptis, homogenitas, derajat halus, daya lekat, derajat keasaman (pH), hedonik/kesukaan, dan Sun Protection Factor (SPF). Hasil penelitian menunjukkan bahwa uji mutu fisik sediaan tabir surya bubuk berbasis ekstrak nanopartikel kulit jeruk bali memenuhi kriteria yang ditetapkan. Ekstrak nanopartikel yang dihasilkan memiliki ukuran 205,2 nm, indeks polidispersitas 0,359, dan zeta potensial -16,0 mV. Nilai SPF dari formulasi tabir surya bubuk berturut-turut adalah 1,617 (minimal), 7,946 (ekstra), 16,554 (ultra), dan 19,072 (ultra), menunjukkan efektivitas perlindungan yang signifikan terhadap sinar UV. Penelitian ini memberikan pengetahuan baru mengenai potensi penggunaan ekstrak tumbuhan sebagai alternatif dalam pengembangan produk tabir surya alami.
Cytotoxicity Test of Bandotan Herbal Ethanol Extract (Ageratum conyzoides L.) Using the Brine Shrimp Lethality Test (BSLT) Method Sari, Farida; Ridwanto, Ridwanto; Daulay, Anny S.; Rani, Zulmai
Jurnal Akademika Kimia Vol. 13 No. 1 (2024)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24775185.2024.v13.i3.pp14-19

Abstract

Cytotoxic activity is a process that can induce cell death. One of the plants that has cytotoxic properties is bandotan (Ageratum conyzoides L.). The purpose of this study was to identify the class of secondary metabolites present in the ethanol extract of Bandotan herb and to evaluate its cytotoxicity by determining the LC50 value using the Brine Shrimp Lethality Test (BSLT) method. This research includes phytochemical screening and cytotoxicity testing of an ethanol extract of Herba bandotan using the BSLT method. BSLT is the first method for cytotoxicity testing using Artemia salina Leach larvae at the nauplii stage. Used in the BSLT method because it has similar responses with mammals, namely DNA-dependent RNA polymerase in Artemia salina Leach, similar to DNA-dependent RNA polymerase in mammals. By phytochemical screening, the secondary metabolites in Herba Bandotan (Ageratum conyzoides L.) are alkaloids, flavonoids, saponins, steroids, and tannins. Based on the results of the cytotoxicity test using the BSLT method, it shows that the ethanol extract of Herba bandotan (Ageratum conyzoides L.) has the potential to be anti-cancer because it has an LC50 value of 255.1915 g / mL, which is included in the toxic category and has the potential to be anti-cancer. Potential as an anticancer agent in the BSLT test if it has an LC50 value < 1000 µg / mL.
Antioxidant Activity of Ethanol Extract of Chinese Petai Peel (Leucaena leucocephala (Lam.) de Wit) Using DPPH (1,1-diphenil-2-picrylhydrazyl) Method Rahmayanti, Putri; Ridwanto, Ridwanto
PCJN: Pharmaceutical and Clinical Journal of Nusantara Vol. 1 No. 03 (2023): PCJN: Pharmaceutical and Clinical Journal of Nusantara
Publisher : CV. Nusantara Scientific Medical

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58549/pcjn.v2i02.71

Abstract

The Chinese petai plant (Leucaena leucocephala (Lam.) de Wit) has been used in various systems of traditional medicine for the treatment of various diseases in humans. The Chinese petai plant (Leucaena leucocephala (Lam.) de Wit) contains antioxidant secondary metabolites that can inhibit oxidation reactions by binding to free radicals and highly reactive molecules. The purpose of this study was to determine the class of chemical compounds contained in the ethanol extract of Chinese petai peel and the antioxidant activity of the ethanol extract of Chinese petai peel (EEKPC). The stages of this research included: collection and processing of simplicia; preparation of the extract by maceration with 70% ethanol; characterization test of simplicia powder; phytochemical screening of ethanol extract of petai cina; antioxidant activity test of EEKPC using the DPPH (1,1-diphenyl-2-pikrilhidrazyl) free radical scavenging method using UV-Vis spectrophotometry at a wavelength of 516 nm. The results of the phytochemical screening examination of Chinese Petai Peel Ethanol Extract (EEKPC) contained alkaloids, flavonoids, saponins, tannins, and triterpenoids. The results of measuring the antioxidant activity of EEKPC showed strength in the "strong" category with an IC50 value of 91.0189 μg/ml.
Characterization and Screening Phytochemicals Orange Purut Leaves (Citrus hystrix) Extract from Kampar, Riau Wijiyaningsih, Dinda; Ridwanto, Ridwanto; Yuniarti, Rafita; Pulungan, Ainil Fithri
PCJN: Pharmaceutical and Clinical Journal of Nusantara Vol. 3 No. 02 (2025): PCJN: Pharmaceutical and Clinical Journal of Nusantara
Publisher : CV. Nusantara Scientific Medical

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58549/pcjn.v3i01.83

Abstract

Orange purut leaves (Citrus hystrix) are one of the shrubs that have high economic value because they contain vitamin C and are often used as a food flavoring. The aim of this research is to determine whether orange purut leaf extract contains phytochemicals and to characterize the properties of orange purut leaf extract. Phytochemical screening of orange purut leaf extract (Citrus hystrix) included the examination of flavonoids, tannins, saponins, steroids/triterpenoids, alkaloids, and glycosides. Phytochemical screening was conducted on both simplicia powder and simplicia extract, and characterization of the simplicia was carried out. The water content of orange purut leaves was found to be 8%, the water-soluble juice content was 37.9%, the ethanol-soluble juice content was 19%, the total ash content was 7.6%, and the acid-insoluble ash content was 0.6%. Orange purut leaf extract positively contains alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids, and glycosides.
Penetapan Kadar Rhodamin B Pada Bumbu Tabur Dengan Metode KLT Dan KCKT Munthe, Ariska; Daulay, Anny Sartika; Ridwanto, Ridwanto; Pulungan , Ainil Fithri
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.845

Abstract

Rhodamine B is a colouring substance in the form of green or reddish-purple crystals, which are odourless and in the form of a bright red solution (fluorescent). Rhodamin B is a colouring substance that can only be used in the textile, paint, paper, or clothing industries, so it cannot be added to food. Rhodamine B is often used as a coloring agent in paper and textiles. This substance is the most dangerous; if consumed, it can cause problems with liver function and liver cancer. The stages of this research include a sample solution, making Rhodamin B standard stock solution, and making a mobile phase. Examination of qualitative analysis using thin layer chromatography and determination of Rhodamine B levels in seasoning performance liquid chromatography method. The results of the research showed that in the qualitative examination, there were two samples containing Rhodamin B. Rhodamin B levels were determined by determining the retention time and calculating Rhodamin B levels using the high-performance liquid chromatography method. The result of deciding Rhodamine B levels in the two samples containing Rhodamin B was sample A; 2,8915 mcg/g  ,sampel B ; 19,6702 mcg/g  Mcg/g. Then, the highest levels of Rhodamin B are found in sample B.
Analisis Kadar Protein Pada Beberapa Jenis Susu Tinggi Protein Dengan Metode Kjeldahl dan Spektrofotometri Visible Syahfitri , Adelya; Daulay, Anny Sartika; Ridwanto, Ridwanto; Yuniarti , Rafita
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.865

Abstract

Introduction: Protein is an essential nutrient that serves as an energy source and plays a crucial role in tissue formation. Protein deficiency can lead to various health disorders, ranging from hair loss to serious conditions such as death. High-protein milk serves as an ideal protein source as it contains essential amino acids and complete nutrients, though its consumption should be adjusted to individual needs to avoid side effects. Research Objectives: This study aims to: (1) analyze protein content in high-protein milk (Whey, Casein, and Gainer) using the Kjeldahl and Visible Spectrophotometry methods, and (2) verify the consistency between analysis results and protein content claims on product packaging. Research Methods: Analysis was conducted using two methods: the Kjeldahl method as a high-precision standard method and Visible Spectrophotometry using Biuret reagent which is more sensitive. Each sample was analyzed with six repetitions to ensure data accuracy. Samples were selected purposively based on best-selling products. Results: The results showed the highest protein content in Whey (525.87 mg/g with Kjeldahl; 873.75 mg/g with Spectrophotometry), followed by Casein (316.87 mg/g; 720.83 mg/g), and the lowest in Gainer (227.5 mg/g; 570.5 mg/g). This consistent pattern indicates conformity with packaging claims, although there were value differences between the two methods. Conclusion: The findings emphasize the importance of verifying protein content in supplement products to ensure nutritional information accuracy for consumers. The differences between the two analysis methods are presumably due to their different analytical principles. This study contributes to developing accurate protein analysis methods for high-protein milk products.  
Co-Authors Abdullah, Husaini Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Ainil Fithri Pulungan Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alistraja Dison Silalahi Alkausar Saragih Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Andi Syahputra, Ricky Ani Sutiani Anjarlit Romiyuliana Br Parapat Annisa Fadilah Amir Annisa Utami Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Asep Trizaldi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Bangun Lubis Cici Andriani Claudy Friesta Melanie Dahlia Hasibuan Dahnia Ayu Fitria Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Elma Natasya Endang D. Hamid Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fadya Azzahra Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fathur Rahman Harun Fatin Fairuz Pasaribu Fatur Rahman Fiqri Hermawan Fithri Pulungan, Ainil FRANSISKA, EVA Gabena Indrayani Dalimunthe Hafizha, Putri Hakimi Hakimi Harahap, Ade Try Atwinda Harahap, Siti Salimah Haris Munandar Nasution Haris Munandar Nst Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Ihsan Fadhilah Imelda Natasia Br S Meliala Indra B. Hutagalung Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi M. Saleh Sitompul Maghfirah , Dini Makhfirah Malahayati Malahayati Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Marissa Tasya Adawiyah Maryanti Yuza Maya Syafira Medhy Ardhita Aprilia Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Al Hudawi Muhammad Al Qodri Muhammad Alfariz Baihaqi Siregar Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Wahyuddin Abdullah Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nomi Noviani Nur Ainun Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Nurmazela, Virda Pitaloka, Syifa Amilia Pulungan, Ainil Fitri Puteri, Cut Intan Annisa Putri Intan Sari Putri Khairani Putri Theresia Harianja Putri Yupa Hasibuan Putriyana Putriyana Qisti Pristiwani Rafita Yuniarti Rahayu , Yayuk Putri Rahayu Rahayu Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmayanti, Putri Reza Irma Ricky Andi Syahputra Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Robiatun Rambe Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Sari, Farida Sarinah Hasibuan Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shakila Salwa Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin Siregar , Anisah Siti Rahmasari Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Sri Wahyuni Sumiyarni Harahap Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah, Aulia Tamara Army Tambupolon, Sanya Tanika Utama, Fazrul Amin Vera Estefania Kaban Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani