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Characterization and Screening Phytochemicals Orange Purut Leaves (Citrus hystrix) Extract from Kampar, Riau Wijiyaningsih, Dinda; Ridwanto, Ridwanto; Yuniarti, Rafita; Pulungan, Ainil Fithri
PCJN: Pharmaceutical and Clinical Journal of Nusantara Vol. 3 No. 02 (2025): PCJN: Pharmaceutical and Clinical Journal of Nusantara
Publisher : CV. Nusantara Scientific Medical

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58549/pcjn.v3i01.83

Abstract

Orange purut leaves (Citrus hystrix) are one of the shrubs that have high economic value because they contain vitamin C and are often used as a food flavoring. The aim of this research is to determine whether orange purut leaf extract contains phytochemicals and to characterize the properties of orange purut leaf extract. Phytochemical screening of orange purut leaf extract (Citrus hystrix) included the examination of flavonoids, tannins, saponins, steroids/triterpenoids, alkaloids, and glycosides. Phytochemical screening was conducted on both simplicia powder and simplicia extract, and characterization of the simplicia was carried out. The water content of orange purut leaves was found to be 8%, the water-soluble juice content was 37.9%, the ethanol-soluble juice content was 19%, the total ash content was 7.6%, and the acid-insoluble ash content was 0.6%. Orange purut leaf extract positively contains alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids, and glycosides.
Penetapan Kadar Rhodamin B Pada Bumbu Tabur Dengan Metode KLT Dan KCKT Munthe, Ariska; Daulay, Anny Sartika; Ridwanto, Ridwanto; Pulungan , Ainil Fithri
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.845

Abstract

Rhodamine B is a colouring substance in the form of green or reddish-purple crystals, which are odourless and in the form of a bright red solution (fluorescent). Rhodamin B is a colouring substance that can only be used in the textile, paint, paper, or clothing industries, so it cannot be added to food. Rhodamine B is often used as a coloring agent in paper and textiles. This substance is the most dangerous; if consumed, it can cause problems with liver function and liver cancer. The stages of this research include a sample solution, making Rhodamin B standard stock solution, and making a mobile phase. Examination of qualitative analysis using thin layer chromatography and determination of Rhodamine B levels in seasoning performance liquid chromatography method. The results of the research showed that in the qualitative examination, there were two samples containing Rhodamin B. Rhodamin B levels were determined by determining the retention time and calculating Rhodamin B levels using the high-performance liquid chromatography method. The result of deciding Rhodamine B levels in the two samples containing Rhodamin B was sample A; 2,8915 mcg/g  ,sampel B ; 19,6702 mcg/g  Mcg/g. Then, the highest levels of Rhodamin B are found in sample B.
Analisis Kadar Protein Pada Beberapa Jenis Susu Tinggi Protein Dengan Metode Kjeldahl dan Spektrofotometri Visible Syahfitri , Adelya; Daulay, Anny Sartika; Ridwanto, Ridwanto; Yuniarti , Rafita
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.865

Abstract

Introduction: Protein is an essential nutrient that serves as an energy source and plays a crucial role in tissue formation. Protein deficiency can lead to various health disorders, ranging from hair loss to serious conditions such as death. High-protein milk serves as an ideal protein source as it contains essential amino acids and complete nutrients, though its consumption should be adjusted to individual needs to avoid side effects. Research Objectives: This study aims to: (1) analyze protein content in high-protein milk (Whey, Casein, and Gainer) using the Kjeldahl and Visible Spectrophotometry methods, and (2) verify the consistency between analysis results and protein content claims on product packaging. Research Methods: Analysis was conducted using two methods: the Kjeldahl method as a high-precision standard method and Visible Spectrophotometry using Biuret reagent which is more sensitive. Each sample was analyzed with six repetitions to ensure data accuracy. Samples were selected purposively based on best-selling products. Results: The results showed the highest protein content in Whey (525.87 mg/g with Kjeldahl; 873.75 mg/g with Spectrophotometry), followed by Casein (316.87 mg/g; 720.83 mg/g), and the lowest in Gainer (227.5 mg/g; 570.5 mg/g). This consistent pattern indicates conformity with packaging claims, although there were value differences between the two methods. Conclusion: The findings emphasize the importance of verifying protein content in supplement products to ensure nutritional information accuracy for consumers. The differences between the two analysis methods are presumably due to their different analytical principles. This study contributes to developing accurate protein analysis methods for high-protein milk products.  
Pelapisan (Edible Coating) Kitosan Cangkang Bekicot terhadap Mutu Fisikokimia Terung Belanda (Solanum betaceum) Humairah, Dwina Angelina; Ridwanto, Ridwanto; Dalimunthe, Gabena Indrayani; Pulungan, Ainil Fithri
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.908

Abstract

Background: Chitosan, a natural polysaccharide derived from chitin deacetylation, shows potential as a natural edible coating to extend the shelf life of perishable fruits. Utilizing giant African snail (Achatina fulica) shells as a chitosan source offers a sustainable solution to reduce biological waste while replacing synthetic chemicals in food preservation. Objective: This study aimed to analyze the effectiveness of snail shell chitosan as an edible coating in maintaining the physicochemical quality of tamarillo (Solanum betaceum) during storage. Methods: Chitosan was isolated from snail shells and applied at concentrations of 10%, 20%, and 30%. Antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa was tested using the disc diffusion method. Physicochemical parameters including weight loss, moisture loss, color, pH, total acidity, vitamin C content, and total soluble solids (TSS) were measured over 7 days of storage. Results: Chitosan demonstrated strong to very strong antibacterial activity, with the largest inhibition zones at 30% concentration (29 mm for S. aureus; 33.8 mm for P. aeruginosa). The 30% chitosan treatment yielded optimal results: lowest weight loss (3.15%), moisture loss (5.75%), pH (7.72), total acidity (16.44%), and TSS (1.350). Vitamin C retention was significantly higher in the 30% chitosan group (551.47 mg/100g) compared to control (416.53 mg/100g), confirming its effectiveness in inhibiting oxidation. Conclusion: 30% snail shell chitosan proved effective as an edible coating for maintaining tamarillo's physicochemical quality, while providing a natural and sustainable solution for the food industry.
Perbandingan Kandungan Fenolik dan Aktivitas Antioksidan pada Varian Seduhan Teh dan Varian Teh Kombucha Secara Spektrofotometri Visible Maghfirah , Dini; Pulungan , Ainil Fithri; Ridwanto, Ridwanto; Yuniarti , Rafita
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 3 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i3.952

Abstract

One of the most widely consumed aromatic beverages in the world is tea. Tea shoots are rich in polyphenolic compounds that function as antioxidants. Not many people know that tea can be fermented with a type of bacteria and yeast culture that can be consumed as a drink for health known as kombucha tea. The health benefits of consuming kombucha tea as anti-carcinogenic, anti-diabetic, detoxification, increased immune response, treating stomach ulcers and lowering cholesterol levels as well as high blood pressure. The purpose of this study was to determine the total phenolic content and antioxidant activity as well as the comparison of total phenolic content and antioxidant activity in tea brewing variations with kombucha tea variations. The methods carried out include testing the total phenolic content and antioxidant activity using the DPPH method on tea brewing variants and kombucha tea variants by visible spectrophotometry.mThe results of total phenolic content in black tea, green tea, and oolong tea were 24,480 mgGAE/g, 26,538 mgGAE/g, and 38,427 mgGAE/g, respectively, while kombucha black tea, green tea, and oolong tea were 39,264 mgGAE/g, 58,581 mgGAE/g, and 64,299 mgGAE/g, respectively. Brewed black tea, green tea, and oolong tea have antioxidant activity with IC50 values of 105,68 ppm in teh moderate category, 81,59 ppm in teh strong category, and 56,25 ppm in the strong category, respectively. In kombucha black tea, green tea, and oolong tea have antioxidant activity with IC50 values respectively 101,60 ppm with moderate category, 55,82 ppm with strong category, and 39,69 ppm with very strong category. While teh vitamin C comparator has antioxidant activity with an IC50 value of 3,22 ppm with a very strong category.
Determination of Total Alkaloid Content of Raru (Cotylelobium lanceolatum Craib) Bark Extract Using UV-ViS Spectrophotometry Method andriani, cici; Daulay, Anny Sartika; Ridwanto, Ridwanto; Nasution, Haris Munandar
Indonesian Journal of Science and Pharmacy Vol. 1 No. 1 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i1.10

Abstract

The raru plant (Cotylelobium  lanceolatum Craib) comes from the Dipterocarpaceae family. Raru bark is usually added to the wine to enhance the taste of the wine. raru bark has antidiabetic compounds as a lowering of sugar levels in the blood. The purpose of this study was to determine the alkaloids in the Raru plant (Cotylelobium   lanceolatum  Craib) and to determine the total alkaloid content found in the Raru plant (Cotylelobium  lanceolatum  Craib). The extraction method used is the maceration extraction method using methanol solvent with various concentrations which is then evaporated with a rotary evaporator. Whereas the quantitative test was carried out by means of the viscous extract obtained by adding 2 mL of Phosphate buffer with a pH of 4.7, 2 mL of bromocresol green (BCG), and measured at a wavelength of 272.705 nm. The yield of the extract from a concentration of 95% was 23.0779%, a concentration of 70% was 21.2144%, a concentration of 50% was 20.3274%. The correlation equation obtained from the caffeine curve is y = 0.0508X + 0.008 and the value of r = 0.999, and the levels of alkaloids with different extract concentrations. Actual alkaloid content in raru bark methanol extract 95% was 14.9094 ± 0.3697 mg/g, actual alkaloid content in raru bark methanol extract 70% was 10.0472 ± 0.4594 mg/g, actual alkaloid content in extract 50% raru bark methanol was 6.6260 ± 0.803.
Analysis of Sodium Benzoate Content in the Market Soft Beverages Using Uv-Vis Spectrophotometry Method Akbar, Windi Hari; Pulungan, Ainil Fithri; Ridwanto, Ridwanto; Daulay, Anny Sartika
Indonesian Journal of Science and Pharmacy Vol. 1 No. 1 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i1.15

Abstract

Sodium benzoate is one of the preservatives that are permitted to be used in food and beverages. Research has been carried out with the aim of knowing that carbonated drinks contain the preservative sodium benzoate, knowing the amount of sodium benzoate in soft drinks and to find out the levels of sodium benzoate in soft drinks fulfilling the maximum usage requirements according to BPOM RI Head Regulation No. 36 of 2013. This study used the uv-vis spectrophotometry method. To determine the preservative content of sodium benzoate, a qualitative test was first carried out on the sample using the color reaction method and esterification test. To determine the levels using a UV-Vis spectrophotometer at a wavelength of 269 nm with ethanol p.a as a solvent. The results of the qualitative test using the color reaction method showed that the three samples of soft drinks positively contained the preservative sodium benzoate. To validate this, an esterification test was carried out on the three samples and they produced the smell of Ambon bananas which proved that the three samples positively contained sodium benzoate. For the quantitative test using the Uv-Vis spectrophotometry method at a wavelength of 269 nm using ethanol p.a as a solvent. The results of the analysis showed that the preservative content of sodium benzoate which was calculated for each sample was for a soft drink sample a: 244.93 ± 19.9349 mg/kg. Soft drinks b: 65.3 ± 0 mg/kg, soft drinks. Soft drinks c: 78.6 ± 13.1291 mg/kg. Based on these results, it can be concluded that the levels of sodium benzoate in samples A, B, and C met the standard requirements for maximum usage limits, namely not more than 600 mg/kg.
Effect of Extraction Method on Yellow Wood Rendement and Phenolic Content (Arcangelisia flava (L.) Merr) UV-Vis Spectrophotometry Extract of Ethanol Sasnita, Merida; Daulay, Anny Sartika; Ridwanto, Ridwanto; Nasution, Haris Munandar
Indonesian Journal of Science and Pharmacy Vol. 1 No. 1 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i1.25

Abstract

Indonesia is a country rich in biodiversity. With the wealth of natural resources, there is very much that can be used from flora and fauna to treat various diseases, yellow wood is one of the plants that have medicinal properties and has not been widely studied, yellow wood is efficacious as antimicrobial, antioxidant, antihyperlipidemia, and anticancer. The objective of this study was to determine the class of chemical compounds found in simplicial and extracts of yellow wood plants and to determine the effect of extraction methods on the yield and phenolic levels of ethanol extracts of yellow wood plants. The stages of this research include processing plant materials, making maceration extracts and socletation of yellowwood plants, examination of characteristics, phytochemical screening and determination of total phenolic levels of ethanol extract 96% maceration and socleation of yellow wood (Arcangelisia flava (L.) Merr) by UV-Vis Spectrophotometry method. The highest yield research results are found in the maceration extract yield, this is because the maceration process time is shorter than the socleation process, so that the compound withdrawal process carried out by maceration is more optimal than the socleation method. In addition, the more solvent used, the more extracts produced. The determination of total phenolics is performed by determining the maximum wavelength of gallic acid using UV-Vis spectrophotometry methods. Yellowwood maceration ethanol extract has a total phenolic content of 22.0087 ± 0.8567 mg GAE/g and socleation extract has a total phenolic content of 18.2023 ± 0.1434 mg GAE/g.
Determination Of Total Alkaloid Content Of Yellow Wood (Arcangelisia flava (L.) Merr) Extract Based On Differences In Ethanol Concentration Using Uv-Vis Spectrophotometry Method Yuliana, Rika; Daulay, Anny Sartika; Ridwanto, Ridwanto; Rahman, Fathur
Indonesian Journal of Science and Pharmacy Vol. 1 No. 2 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i2.27

Abstract

Yellow wood with its secondary metabolites is one of the plants that has the potential as a traditional medicinal plant to treat various diseases, such as cancer. The chemical compounds contained in yellow wood are alkaloids, saponins, tannins, flavonoids, steroids/triterpenoids. The objective of this research was to determine the chemical compounds contained in ethanol extract and determine the total alkaloid value of yellow wood extract. This research method is an experimental method which includes processing plant materials, making ethanol extract concentrations of 50, 70 and 96%. Characterization examination, phytochemical screening and determination of total alkaloid levels of yellow wood extract by Uv-Vis spectrophotometry method. The results of phytochemical screening on yellow wood ethanol extract contain chemical compounds such as alkaloids, saponins, tannins, flavonoids, steroids/triterpenoids. Determination of total alkaloid levels is carried out by determining the maximum wavelength of caffeine, measuring the kaffein calibration curve and calculating total alkaloid levels using the Uv-Vis spectrophotometry method. Results The determination of total alkaloid levels in ethanol solvent extract concentration of 50% is 2.9649 ± 0.1767%, ethanol solvent extract with a concentration of 70% is 6.0180 ± 0.2469% and ethanol solvent extract with a concentration of 96% is 10.92001 ± 2.7803%.
Cytotoxicity Test of Ethanol Extract of Herb Sintrong (Crassocephalum crepidioides (Benth.) S. Moore) Against Artemia Salina Leach Shrimp Larvae Using the BSLT Method Tambupolon, Sanya Tanika; Ridwanto, Ridwanto
Indonesian Journal of Science and Pharmacy Vol. 1 No. 2 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i2.33

Abstract

Cancer is a disease that arises as a result of an error in the division system at the cell level resulting in abnormal cell growth with a continuous, uncontrolled growth rate that can change shape and spread to other organs or is called metastasis. One of the plants that has the potential as an anticancer is the herb sintrong (Crassocephalum crepidiodes (Benth.) S. Moore). The purpose of this study was to determine the class of secondary metabolites contained in the powder and ethanol extract of the herb sintrong (Crassocephalum crepidioides (benth.) S. Moore) and its cytotoxicity by looking at the LC50 value using the Brine Shrimp Lethality Test (BSLT) method.Cytotoxicity testing was carried out using the BSLT method with Artemia salina Leach shrimp larvae as test animals with concentrations of 100-1000 µg/mL, with negative control without added extract. The LC50 value was analyzed using a straight line regression equation by entering data on the probit value and the logarithm of the concentration.The secondary metabolite compounds contained in sintrong herb (Crassocephalum crepidiodes (Benth.) S. Moore) using the phytochemical screening method are flavonoids, alkaloids, tannins, saponins and steroids. Based on the results of the toxicity test using the Brine Shrimp Lethality Test (BSLT) method, the LC50 value = 295.3800 µg/mL showed that the sintrong herb extract is toxic and has the potential as an anticancer. The test compound is said to be toxic if the LC50 value is ˂1000 µg/mL.
Co-Authors Abdullah, Husaini Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Andi Syahputra, Ricky Ani Sutiani Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Bangun Lubis Cici Andriani Claudy Friesta Melanie Dahlia Hasibuan Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Dison Silalahi, Alistraja Elma Natasya Endang D. Hamid Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fathur Rahman Harun Fatur Rahman Fithri Pulungan, Ainil FRANSISKA, EVA Gabena Indrayani Dalimunthe Hafizha, Putri Hakimi Hakimi Harahap, Ade Try Atwinda Harahap, Siti Salimah Harahap, Sumiyarni Haris Munandar Nasution Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Imelda Natasia Br S Meliala Indra B. Hutagalung Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi Maghfirah , Dini Makhfirah Malahayati Malahayati Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Maryanti Yuza Maya Syafira Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Alfariz Baihaqi Siregar Muhammad Amin Nasution Muhammad Wahyuddin Abdullah Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nst, Haris Munandar Nur Ainun Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Nurmazela, Virda Parapat , Anjarlit Romiyuliana Br Pasaribu, Fatin Fairuz Pitaloka, Syifa Amilia Pulungan, Ainil Fitri Puteri, Cut Intan Annisa Putri Intan Sari Putri Khairani Putri Theresia Harianja Putri Yupa Hasibuan Putriyana Putriyana Qisti Pristiwani Rahayu , Yayuk Putri Rahayu Rahayu Rahma Dona, Rahma Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmayanti, Putri Rambe, Robiatun Reza Irma Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Saragih, Alkausar Sari, Farida Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin Siregar , Anisah Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah, Aulia Tamara Army Tambupolon, Sanya Tanika Trizaldi, Asep Utama, Fazrul Amin Vera Estefania Kaban Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani