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UV–Vis Quantification of β-Carotene in Spinach under Raw and Boiled Conditions Damanik, Sumayyah; Sartika Daulay, Anny; Ridwanto, Ridwanto; Harun, Fathur Rahman
Indonesian Journal of Pharmaceutical Education Vol 5, No 3 (2025): September–December 2025
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v5i3.32374

Abstract

β-Carotene is a provitamin A with strong antioxidant activity but is unstable to heat and oxidation. This study aimed to determine the β-carotene content of green spinach (Amaranthus viridis L.) and red spinach (Amaranthus tricolor L.) under fresh and boiled conditions using UV–Vis spectrophotometry. The assay was performed at 451 nm after maceration extraction with n-hexane:acetone:ethanol (2:1:1, v/v). Calibration in the range of 4–10 μg/mL showed excellent linearity (y = 0.064x – 0.003, r = 0.999). The results demonstrated that red spinach contained substantially higher β-carotene than green spinach. Boiling slightly increased the content in green spinach (17.16 → 18.76 ± 0.21 mg/g), while it caused a small decrease in red spinach (50.23 → 47.76 mg/g). These findings suggest that boiling has different effects depending on the spinach type, possibly due to a balance between heat-induced degradation and enhanced release from softened plant tissues. In conclusion, red spinach is a richer dietary source of β-carotene, but thermal processing reduces its stability. The validated UV–Vis spectrophotometric method is suitable for routine determination of β-carotene in leafy vegetables.
Problematika Auditing Syariah Dalam Pelaksanaan Shariah Compliance di Lembaga Keuangan Syariah: Sebuah Meta-Sintesis Ridwanto, Ridwanto; Abdullah, Muhammad Wahyuddin; Muchlis, Syaiful
Al-Sharf: Jurnal Ekonomi Islam Vol 4, No 3 (2023)
Publisher : Yayasan Rahmat Islamiyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56114/al-sharf.v4i3.11276

Abstract

Sharia financial institutions have a central role in the financial system based on the Sharia principles. Meanwhile, sharia audit as a monitoring tool has a crucial role in ensuring sharia compliance. In actualize their responsibilities, sharia auditors have several problems. This article aims to provide a more comprehensive brief insight into the problems of sharia auditing and offers practical solutions to increase compliance with Sharia values and principles in Indonesian sharia financial institutions. This article uses a meta-synthesis method to identify the main challenges in implementing sharia auditing in sharia financial institutions. The findings of the research results show that the primary challenges for sharia auditing in implementing sharia compliance are the lack of qualified sharia auditors, lack of sharia audit frameworks and standards, and also the lack of providing educational levels to the sharia supervisory board. The proposed solution involves enhancing the quality of auditors through training and certification, improving the sharia audit framework and standards, and also developing special education for the Sharia Supervisory Board.
Bahaya Riba Dalam Ekonomi Islam Di Indonesia: Sebuah Meta-Sintesis Ridwanto, Ridwanto; Bulutoding, Lince; Sumarlin, Sumarlin
Al-Sharf: Jurnal Ekonomi Islam Vol 5, No 1 (2024)
Publisher : Yayasan Rahmat Islamiyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56114/al-sharf.v5i1.11410

Abstract

Penelitian ini membahas bhaya riba dalam ekonomi Islam di Indonesia dengan pendekatan meta-sintesis. Riba, yang dianggap sebagai praktik haram dalam Islam, memiliki dampak negatif yang signifikan dalam konteks ekonomi Indonesia. Dalam penelitian ini, metode meta-sintesis digunakan untuk mengungkapkan pemahaman yang lebih mendalam tentang dampak riba dalam ekonomi Islam dengan analisis dan interpretasi yang mendalam. Hasil dari sejumlah penelitian terdahulu diidentifikasi untuk memberikan pemahaman yang lebih komprehensif tentang masalah ini. Beberapa temuan utama mencakup dampak riba sebagai penghambat investasi, faktor bertambahnya utang negara, dan penyebab ketidakstabilan ekonomi. Penelitian ini memberikan wawasan yang mendalam tentang bagaimana riba mempengaruhi setiap aspek ekonomi Indonesia dan menyoroti pentingnya menghilangkan riba dalam upaya menciptakan sistem ekonomi yang sesuai dengan prinsip dan nilai-nilai Syariah dalam masyarakat Indonesia.
Utilization Of Natural Materials As Universal Natural Food Colors Red Yellow Green In The Community Of Sidomulyo Village Biru-Biru District: Pemanfaatan Bahan Alam Sebagai Pewarna Pangan Alami Universal Merah Kuning Hijau Pada Masyarakat Desa Sidomulyo Kecamatan Biru-Biru Sartika Daulay, Anny; Ridwanto, Ridwanto; Dison Silalahi, Alistraja; Wahyuni, Sri; Indrayani Dalimunthe, Gabena; Fadhilah M, Ihsan
Jukeshum: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2025): Edisi Januari 2025
Publisher : Universitas Haji Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51771/jukeshum.v5i2.1012

Abstract

Food coloring is an additional ingredient used to produce food products so they are more attractive to consume. Synthetic food coloring comes from chemicals, can contain dangerous metals and/or benzene rings which have the potential to cause various diseases in humans. Meanwhile, natural dyes are obtained from natural ingredients so they are safer to use. The aim of this community service is to provide understanding to the public about the benefits of plants in the form of fruit, leaves and fruit skins that are no longer used as natural food coloring. This community service (PkM) is carried out by socializing the use of fruit and/or fruit waste, fresh leaves which are commonly found in the community, such as dragon fruit peel as waste. Other ingredients used are turmeric rhizomes and bay leaves, suji leaves and pandan leaves. Socialization of making food coloring materials is carried out using electronic media such as videos. Meanwhile, the explanation of the material is carried out using the presentation and discussion method. Based on the results of the implementation of this PkM, the people of Sidomulyo Village, Biru-Biru District, were very enthusiastic and eager to take part in this activity. The people of Sidomulyo Village can understand and comprehend the dangers of using synthetic dyes and understand the benefits of using natural dyes in food produced in the household. Then in the future, the results of this community service can be applied to products that can be marketed commercially to increase family income.
Uji Aktivitas Antioksidan Ekstrak Etanol Daun Gandarusa (Justicia gendarussa Burm. F.) di Daerah Sibolga, Sumatera Utara Dengan Metode DPPH (1,1-Diphenyl-2-Picrylhydrazil) Harahap, Ade Try Atwinda; Ridwanto, Ridwanto
Journal of Health and Medical Science Volume 3 Nomor 1 Januari 2024
Publisher : CV. Pusdikra Mitra Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51178/jhms.v3i1.1874

Abstract

Tumbuhan gandarusa yaitu untuk pengobatan. Bagian tanaman yang digunakan adalah daun dan akar baik yang segar ataupun yang telah dikerinbgkan. Daunnya berkhasiat untuk mengatasi batuk, asma, nyeri lambung, rematik sendi, nyeri pinggang (encok), obat pening dan obat untuk haid yang tidak teratur. Kegunaan yang lain untyuk obat luka trpukul (memar), patah tulang, reumatik pada persendian, bisul, borok dan korengan. Tujuan:Tujuan penelitian ini untuk mengetahui aktivitas antioksidan ekstrak etanol daun Gandarusa dan untuk mengetahui golongan senyawa dalam daun gandarusa dan juga nilai IC50. Ekstrak didapat dengan cara maserasi menggunakan pelarut etanol 96%. Metode: Ekstrak etanol daun Gandarusa diuji menggunakan metode DPPH (1,1-Diphenyl-2-Picrylhydrazil), dan menggunakan spektrofotometri UV - Visible untuk menentukan panjang gelombangnya. Hasil skrining fitokimia bahwa serbuk simplisia daun Gandarusa mengandung golongan senyawa alkaloid, flavonoid, saponin, triterpenoid, dan tannin. Hasil pengujian aktivas antioksidan dalam meredam radikal bebas DPPH menunjukkan bahwa ekstrak etanol daun Gandarusa memiliki aktivitas antioksidan kategori kuat dimana ekstrak etanol daun Gandarusa memiliki nilai IC50 sebesar 59,46 ppm. Kesimpulan:Hasil pengujian aktivasi antioksidan dalam meredam radikal bebas DPPH menunjukkan bahwa ekstrak etanol daun Gandarusa memiliki aktivitas antioksidan kategori kuat.
Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol daun alpukat (Persea americana Mill.) dengan metode DPPH Rahmah, Ruhiya; Rahayu, Rahayu; Ridwanto, Ridwanto; Daulay, Anny Sartika
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.369

Abstract

Indonesia is a country that is famous for its wealth of natural energy sources and plants that have medicinal properties. Avocado is one of the plants that residents have used from generation to generation for traditional medicine. Avocado (Persea americana Mill.) is a plant that contains many compounds that have antioxidant properties. Secondary metabolite bioactive compounds that play a role are saponins, alkaloids, flavonoids, steroids, safrole, and tannins. Antioxidant compounds are electron distributors, which can counteract oxidants' negative impacts. This research aims to determine the ethanol extract content of avocado leaves containing secondary metabolites and the IC50 value of avocado leaf extract. One method of measuring free radicals by antioxidant compounds is to use the DPPH (1,1-diphenyl-2-picrylhydrazyl) method with a UV-Vis spectrophotometer at a wavelength of 516 nm and vitamin C as a comparison. The research showed positive results in the phytochemical screening of Simplicia and avocado leaf extract against alkaloids, flavonoids, tannins, steroid saponins, and glycosides. It can be concluded that the results of research on the antioxidant activity of avocado leaves have an IC50 value of 9.24 µg/ml in the very strong category, equivalent to vitamin C comparison, which has antioxidant activity with an IC50 value of 1.942 µg/ml in the very strong category.
Skrining fitokimia dan uji aktivitas antioksidan ekstrak metanol daun salam (Syzygium polyanthum (Wight.) Walp.) dari Gampong Bunot, Pidie Jaya dengan metode DPPH Nazirah, Nazirah; Nasution, Muhammad Amin; Ridwanto, Ridwanto; Nasution, Haris Munandar
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.376

Abstract

Antioxidants are compounds that can delay, slow down, or inhibit the oxidation reaction of food or medicine. Antioxidants include electron-donor compounds that work by donating an electron to compounds that are free radicals so that radical activity is inhibited. One of the plants that can be used as a source of natural antioxidants is salam leaf (Syzygium polyanthum (Wight.) Walp). Salam leaves contain secondary metabolite compounds, including alkaloids, tannins, saponins, steroids, triterpenoids, and flavonoids, which act as antioxidants. The study aimed to determine whether the methanol extract of salam leaves (Syzygium polyanthum (Wight.) Walp.), which grows in Gampong Bunot, Pidie Jaya, contains secondary metabolites and has activity as an antioxidant. This research was conducted experimentally, where the sample used was salam leaves. The stages of this research included the processing of plant materials, preparation of methanol extract of salam leaves, characterization examination, phytochemical screening, and antioxidant activity test of methanol extract of salam leaves using the DPPH method. Salam leaf extract was prepared by maceration method using methanol solvent obtained and concentrated with a rotary evaporator, then the phytochemical screening test and the antioxidant activity test of the methanol extract of salam leaves were carried out using the DPPH method (1,1 diphenyl-2-picrylhidrazil) at a wavelength of 516 nm and vitamin C as a comparison. Based on the results of the phytochemical screening test, there were differences in secondary metabolites in the methanol extract of salam leaves grown in Gampong Bunot, Pidie Jaya, including alkaloids, flavonoids, saponins, tannins, steroids, and glycosides. The results of the antioxidant activity test of the methanol extract of salam leaves obtained the IC value50 of 25.68 µg/ml and the IC value50 Vitamin C of 4.537 µg/ml in the second category is very strong.  
Skrining fitokimia dan uji aktivitas antioksidan dari ekstrak etanol daun kedondong (Spondias dulcis Soland. ex Forst. fil) dengan metode DPPH Yanti, Rahma; Nasution, Muhammad Amin; Ridwanto, Ridwanto; Nasution, Haris Munandar
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.377

Abstract

Antioxidants are compounds that can ward off free radicals and prevent damage caused by free radicals. Free radicals are unstable and reactive molecules formed from unpaired electrons that can cause damage to the skin. To protect the body's skin from free radicals, it can be overcome by using an antioxidant compound. One is kedondong leaves, which contain polyphenolic compounds known to have antibacterial and antioxidant properties. This study aimed to conduct phytochemical screening and determine the antioxidant activity of ethanol extract of kedondong leaves with the DPPH method. This study was conducted experimentally, where the sample used was kedondong leaves. The stages of research include sample preparation, plant identification, making simplisia, phytochemical screening on kedondong leaf extract and simplisia, then antioxidant activity tests were carried out on kedondong leaf ethanol extract using the DPPH method (1,1-diphenyl -2-picrylhydrazil) at a maximum wavelength of 516 nm, as a positive control vitamin C was used. The research on phytochemical screening showed that ethanol extract of kedondong leaves contains secondary metabolite compounds, namely tannins, saponins, alkaloids, flavonoids, steroids, triterpenoids, and glycosides. In testing, the antioxidant activity of ethanol extract of kedondong leaves showed very strong antioxidant activity with an IC50 of 44.855 μg/ml, and vitamin C had very strong antioxidant activity with an IC50 of 4.44 μg/ml.
Penetapan kadar flavonoid total ekstrak kayu kuning dari daerah Samarkilang Aceh Tengah dengan berbagai konsentrasi etanol menggunakan metode spektrofotometri visible Afrizani, Afrizani; Daulay, Anny Sartika; Ridwanto, Ridwanto; Rahman, Fathur
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.391

Abstract

The yellow wood sampled was taken from the Samarkilang area of Central Aceh. The use of yellow wood plants as herbal medicinal ingredients. The chemical compounds contained in Yellow Wood are alkaloids, phenolics, flavonoids, saponins, and tannins. Phenolic compounds and flavonoids have activity as antioxidants. The purpose of this study was to determine the chemical compounds contained in the ethanol extract and to determine the total flavanoid value of the ethanol extract of the yellow wood (Arcangelisia flava (L.) Merr). This research method is an experimental method which includes processing plant materials, making 50%, 70%, and 96% ethanol extracts. Examination of characterization, phytochemical screening and determination of total flavonoid content of the ethanol extract of Kayu kuning using the Uv-Vis spectrophotometry method. Yellow wood extract was prepared by maceration method using various ethanol concentrations of 50%, 70% and 96% the extract obtained was concentrated with a rotary evaporator. Furthermore, the determination of total flavonoid levels was carried out using the Uv-Vis spectrophotometry method. The results of the phytochemical screening on the ethanol extract of Kayu kuning contained groups of chemical compounds such as alkaloids, flavonoids, saponins, tannins, and steroids/triterpenoids. Determination of total flavonoid content was carried out by determining the maximum wavelength of quercetin, operating time, measuring the quercetin calibration curve and calculating total flavonoid content using the Uv-Vis spectrophotometry method. The results of determining total flavanoid levels in ethanol solvent extract with a concentration of 50% 0,96882 ± 0,2157 mgQE/g, in ethanol solvent extract with a concentration of 70% was 2,6693 ± 11,7041 mgQE/g, and in ethanol solvent extract with a concentration of 96 % is 3,838 ± 1,3036 mgQE/g.
Aktivitas antioksidan dan uji toksisitas ekstrak kulit batang kayu raru (Cotylelobium lanceolatum Craib) dengan menggunkan metode DPPH dan metode BSLT Rossa, Aliffa; Daulay, Anny Sartika; Ridwanto, Ridwanto; Rahayu, Yayuk Putri
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.436

Abstract

Raru (Corylelobium lanceolatum Craib) is a group of endemic tropical forest plants from the Dipterocarpaceae family in Indonesia. Raru is a term for a group of types of bark added to palm sap, which aims to increase the taste and alcohol content of the drink. People in Sumatra believe that raru bark (Cotylelobium lanceolatum Craib) can be used as an antidiabetic drug. This study aims to determine the antioxidant activity of raru bark, the toxicity activity of raru bark extract, and the secondary metabolites in raru bark. This research was conducted in descriptive was born with a descriptive method. The sample used was raru bark. The research phase included sample preparation, plant identification, simplicial manufacture, antioxidant activity test, DPPH standard stock preparation, blank solution preparation, phytochemical screening, antioxidant activity test based on IC50, Toxicity test, and toxicity activity test based on LC50 value. The result obtained in testing the antioxidant activity of raru bark had an IC50 value of 94.25 µg/mL in the vary strong category. While the result of the probit analysis for the toxicity of raru bark extract using the BSLT method obtained an LC50 value of 350.9942 µg/mL. It is in the toxic category and has the potential to be an anticancer.
Co-Authors Abdullah, Husaini Abdullah, Muhammad Wahyuddin Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Ani Sutiani Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Asep Trizaldi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Cici Andriani Claudy Friesta Melanie Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Dison Silalahi, Alistraja Elma Natasya Eva Fransiska Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fatur Rahman Fithri Pulungan, Ainil Gabena Indrayani Dalimunthe Hafizha, Putri Harahap, Ade Try Atwinda Harahap, Siti Salimah Harahap, Sumiyarni Haris Munandar Nasution Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Imelda Natasia Br S Meliala Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi Maghfirah , Dini Makhfirah Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Maryanti Yuza Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nst, Haris Munandar Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Parapat , Anjarlit Romiyuliana Br Pitaloka, Syifa Amilia Puteri, Cut Intan Annisa Putri Khairani Putri Theresia Harianja Putriyana Putriyana Qisti Pristiwani Rafita Yuniarti Rahayu , Yayuk Putri Rahayu Rahayu Rahma Dona Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmayanti, Putri Reza Irma Ricky Andi Syahputra Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Robiatun Rambe Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Saragih, Alkausar Sari, Farida Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin, Siradjuddin Siregar , Anisah Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah Aulia Tamara Army Tambupolon, Sanya Tanika Utama, Fazrul Amin Vera Estefania Kaban Virda Nurmazela Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani