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SENSITIVITY OF APPLE MANGO LEAVES (Mangifera indica) SOLUTION TOWARD Edwardsiella tarda Yosri Yosri; Henni Syawal; Morina Riauwaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was conducted from Juni to September 2018, atParasites and Fish Disease Laboratory, Faculty of Fisheries and Marine, University of Riau. The Purpose of this study was to determine the sensitivity of apple mango leaves (Mangifera indica) solution as antimicrobial to Edwardsiella tarda, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) of apple mango leaves (Mangifera indica) solution against to  (Pangasiussp.) with length 8-10 cm by immersion for 24 hours. Sensitivity test is using the method of Kirby-Bauer disk blank 6 mm disc with dose used were 100%, 90%, 80% , 70%, 60%, 50%, 40%, 30%, 20%, 10%,9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1% and control Tetracyclin. The result shown that the apple mango leaves solution can inhibit the growth of bacteria Edwardsiella tardathat in the dose of 1% with 6,05 mm clear zone . MIC test showed this resueld is 2% has a number of colonies of bacteria aqual 282.33 CFU/mL is able to inhibit the growth of bacteria Edwardsiella tarda. Dose LD50apple mango leaves solution against catfish by immersion for 24 hours in 2% .Keywords: Apple Mango Leaves, Sensitivity, Minimum Inhibitory Concentration
LEUKOCYTES DIFFERENTIATION OF Pangasius hypophthalmus WHICH INFECTED BY Aeromonas hydrophilaAND TREATED WITHRhizophora sp. Chandra Chandra; Henni Syawal; Iesje Lukistyowati
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Rhizophora sp. is one of the mangrove plant which potentially for antibacterial compound, because it has an alkaloids, saponins, flavonoids and tannins. This research purpose was to know the proper dose of Rhizophora sp. leaves extract in healing the MAS disease (Motile Aeromonas Septicaemia), which can be seen through the leukocytes differentiation of Pangasius hypophthalmus. The method in this research was the experimental method using the Completely Randomized Design (CRD) with one factor, four treatment levels and three times replication. The treatments were P0 (without immersion)and the immersion with leaves extract at dose P1 (1300 ppm), dose P2 (1600 ppm), and dose P3 (1900 ppm). The immersion was implemented twice in 24 hours for about 5 minutes. The fishes were used at 13-15 cm of length for 120 fishes which were infected by leaves extract. The results showed that the immersion of Rhizophora sp.leaves extract at dose 1600 ppm of leaves extract dose could treat the MAS disease (Motile Aeromonas Septicaemia) of Pangasius hypophthalmus. The total average of leukocytes was 69,61×103cell/mm3, leukocytes differentiation was 81,33% of lymphocytes, 9,66% of neutrophils, 9,00% of monocytes. The fagocyte index was 36,66% and the survival rate was 84,61%.Key Words : Leukocytes, Pangasius hypophthalmus,Rhizhopora sp.leaves extract
LEUKOCYTES DIFFERENTIATION PROFIL OF TILAPIA (Oreochromis niloticus) THAT WERE INFECTED BY ECTOPARASITES IN POND IN SIMPANG TIGA SUB-DISTRICT PEKANBARU Roslina '; Henni Syawal; Morina Riauwaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was conducted in Oktober to Desember 2015 in the Laboratory of Parasite and Fish Disease of Fisheries and Marine Science Faculty, University of Riau. This study aims to understand the leukocytes differentiation Profil of tilapia (Oreochromis niloticus) that were infected by ectoparasites in Simpang Tiga sub-district Pekanbaru. The research method used in this study is a survey method by taking sampels in the field and fish sample will be analized in the laboratory, and will be identificied based on Kabata, 1995. The hematologycal parameter (leukocytes differentiation) will be measured based on Swicki and Anderson, 1974. The result shown that three types of ectoparasite that were infected in tilapia was Trichodina sp., Dactylogyrus sp., and Gyrodactylus sp. The total leukocytes of male tilapia was 92.350-103.400 cells/mm3 and famale tilapia was 89.375-100.200 cells/mm3. Differentiation of leukocytes such as lymphocytes of male tilapia was 65-73,5%, whereas the famale tilapia was 61,5-77,5%, monocytes of male tilapia was 22-31,5%, whereas the famale tilapia was 18-34,5%, and neutrophils of male tilapia (3-4,5%) was higher than famale tilapia (4-5%).Keywords : Ectoparasites, Differentiation, Leukocytes, Tilapia, Pekanbaru
Sensitivity of Mangosteen Rind (Garcinia mangostana L.) Solution Toward Aeromonas hydrophila Dian Fitria M; Iesje Lukistyowati; Henni Syawal
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTResearch was conducted from September to October 2014, at the labory Examination of Fish Disease, Fisheries and Marine Sciences Faculty of Riau University. The Purpose of this study was to determine the sensitivity of mangosteen rind (G. mangostana L.) as antimicrobial to A. hydrophila, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) mangosteen rind (G. mangostana L.) solution against to catfish (Clarias gariepinus) (10-13 cm) by immersion. The research method used doses 10 % (19 g/L), 20 % (38 g/L), 30 % (57 g/L), 40 % (76 g/L), 50 % (95 g/L), 60 % (114 g/L), 70 % (133 g/L), 80 % (152 g/L), 90 % (171g/L), 100 % (190 g/L). The results showed that mangosteen rind was effectively to inhibit the growth of bacterial A. hydrophila at a dose of 50 % (95 g/L) inhibitory zone of 13,65 mm, with the number of A. hydrophila colonies in TSA medium dose 50 % (95 g/L) is 30,6 x 108 cfu/mL. LD50 test was obtained in the dose mangosteen rind 4, 43 g/L.Key words : Garcinia mangostana L., Aeromonas hydrophila, antimicrobial, lethal dose
HISTOPATHOLOGY OF LIVER AND GUT OF Pangasius hypopthalmus THAT WERE FEED WITH CURCUMIN EXTRACT (Curcuma domestica) AND WERE INFECTED WITH Aeromonas hydrophila Zulaiha '; Morina Riauwaty; Henni Syawal
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
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Pangasius hypopthalmus is one fish commodity that were known as commodity prospected and demand by employers to cultivate. The aimed this research was to understand the histopathological changes of liver and gut of Pangasius hypopthalmus that were feed with curcumin extract (Curcuma domestica) and were infected Aeromonas hydrophila. This research has been conducted in the Laboratory of Microtechnic of the Mathematics and Natural Sciences Faculty and Laboratory of Parasites and Disease of the Fisheries and Marine Science Faculty, Riau University Pekanbaru in Juny to October 2016. This study used five treatments, Kn: Negative control (feeding without the addition of curcumin and uninfected bacteria A. hydrophila), Kp: A positive control (feeding without the addition of curcumin and infected by bacteria A. hydrophila), P1: feed with curcumin addition of 0.3 g / kg, P2: Feed with the addition of curcumin of 0.5 g / kg, P3: feed with curcumin addition of 0.7 g / kg. observation tissue with histology studies and observed under a microscope. The results of the study showed that this structure of Pangasius hypopthalmus liver visible damage such as necrosis, haemorrhage, fatted degeneration, and hypertrophy. While the structure of Pangasius hypopthalmus gut visible damage such as haemorrhage, cell infiltration and goblet cell proliferation. The best results was P3 the addition of curcumin extract added to the feed with concentration of 0.7 g / kg of feed.Key words: Pangasius hypopthalmus, Liver, Gut, histopathology, Curcumin
The Effect Vitamin C Enriched Pellets to Increase the Immunity of Oreochromis niloticus Maytra Wirdona; Morina Riauwaty; Henni Syawal
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
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ABSTRACTVitamin C is a nutrient that is soluble in water and is essential for life as well as for maintaining health, enhancing immunity of fish in fighting infections and as an antioxidant. The study aims to obtain the best dose of vitamin C on total leukocytes, leukocyte differentiation, and phagocytosis activity to increase the immunity of nile tilapia towards disease attack Streptcoccosis. The method used is the experimental method by using the full random draft (RAL) One factor with five levels of treatment and the treatment is repetead three times. After the treatment was applied, namely Kn (without vitamin C enriched pellets and not challenged), Kp (pellets without vitamin C enriched and challenged), vitamin c enriched pellets at a dose of P1 (1 g/kg and challenged with Streptococcus agalactiae), P2 (1.5 g/kg and challenged with S. agalactiae), P3 (2 g/kg and challenged with S. agalactiae). Nile tilapia used in size 8-10 cm with solid tebar 1 tail/3 L water is maintained in the aquarium with a size of 40 x 30 x 30 cm with a feed enriched with vitamin C for 46 days. The fish was challenged with S. agalactiae with a density 108 CFU/mL at the dose of 0.1 mL/tail intramuscular on day 32 after that the fish re-maintained until the 46 days. Measured parameters are total leucocytes, leucocyte differentiation, phagocytic activity and survival rate of nile tilapia. The results shown that the feed enriched with vitamin C and tested with S. Agalactiae has an influence on the immunity of fish with the best treatment in P3 (the vitamin C enriched pellets at a dose of 2 g/kg and challenged) with an average total leukocytes was 8.02 x 104 cells/mm3, lymphocytes were 84.67%, neutrophils was 8.67%, monocytes were 6.66%, phagocytosis activity is 46.00% and the livelihood reaches 93.33%.Keywords : Leukocytes, Tilapia, Vitamin C, Streptococcus agalactiae, Phagocytosis 
Hematologycal of Jambal siam (Pangasius hypophthalmus) That Using Aeromonas hydrophila Vaccine Rika Sinaga; Morina Riauwaty; Henni Syawal
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT The vaccine is alternative to controlling infection of Aeromonas hydrophila. The purpose of this research was to given understand the  hematology of (Pangasius hypophthalmus) that was with  Aeromonas hydrophila vaccine. This research was conducted  in April to June 2018. The method was an experimental method with a Completely Randomized Design (CRD) one factor with five treatments level and three times replication. The treatments were: Kn: The fishes that was not with given vaccine and   infected by Aeromonas hydrophila, Kp: The fishes that was not given vaccine and was infected by Aeromonas hydrophila, P1: Soaked with vaccine dose 1 ml/10 L water for 15 minutes, P2: Soaked with vaccine dose 1 ml/10 L water for 30 minutes, P3: Soaked with vaccine dose 1 ml/10 L water for 45 minutes. Pangasius hypophthalmus at average 8-10 cm of leght for 250 fishes. The challenge test was carried out for 22 days by Aeromonas hydrophila, the purpose of which was to determine imumunity of fish. The result shown that the total erythrocyte, hematocryte value, hemoglobin level, relative percent survival (RPS), antibody titer. The result swowed that immersion of the vaccine for 30 minutes could disease Motile Aeromonas Septicaemia. The results obtained from the research are, the  average of total erythrocyte is 245x104 cells/mm3, hematocryte value 28,00%, hemoglobin level 9,50 g/dl, relative percent survival (RPS) 83,33%, antibody titer 0,822%. Keywords : Hematology, Pangasius hypophthalmus, HydroVac vaccine, Aeromonas hydrophila, erythrocyte.
HEMATOLOGYCAL CONDITION OF TILAPIA (Oreochromis niloticus) THAT WERE INFECTED BY ECTOPARASITES IN PONDS IN SIMPANG TIGA SUB-DISTRICT PEKANBARU Isnaini Lidya Rizki; Morina Riauwaty; Henni Syawal
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis research was conducted in July to September 2014 in Parasite and Fish Diseases Laboratory of Fisheries and Marine Science Faculty, Riau University. The aim of this research to understand the hematologycal condition of tilapia (Oreochromis niloticus) that were infected by ectoparasites. The method used in this study is a survey method by taking fish samples from one location and the fish sample will be taken in the laboratory and identified. Ectoparasite examinations will be done based on Kabata, 1985 and hematologycal parameter will be measured based on Siwicki and Anderson, 1993. Result shown that the three types of ectoparasites that were infected in tilapia are: Trichodina sp., Dactylogyrus sp., and Gyrodactylus sp. The average of hematocrit value of male tilapia was 19,7- 22,18%, the hemoglobin concentration was 4.07-4.8%, and the total erythrocyte was 1.483.000-1.680.000 cells / mm3, whereas the hematocrit of female tilapia was 18,9-21,6%, the hemoglobin concentration was 3,8-4,9%, the total erythrocytes was 1.346.000-1.562.500 cells / mm3. It can be concluded that the hematologycal conditions of tilapia (male and female) in that area was different.Keywords : Ectoparasites, Hematologycal, Tilapia, Pekanbaru
HISTOPATHOLOGY OF GILL AND BRAIN OF TILAPIA (Oreochromis niloticus) THAT WERE INFECTED WITH Streptococcus iniae Marwati '; Morina Riauwaty Siagian; Henni Syawal
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on July until Desember 2015 at Parasite and Fish Disease Laboratory, Faculty of Fisheries and Marine Sciences, University of Riau. The aims of this research was to determine a gill and brain tissue changes of Tilapia (O. niloticus) infected by S. iniae with different concentration. This research used experimental method, infected with according to treatment and were analyzed. The treatments applied were intraperitoneal infection by S. iniae with different concentration, they were ; Kn (negative control, were not infected by S. iniae), P1 (infection by S. iniae with concentration 109 CFU/mL), P2 (infected by S. iniae with concentration 107 CFU/mL), P3 (infected by S. iniae with concentration 105 CFU/mL), and than fishes were reared for 7 days. Gill tissue showed hyperplasia, fusion in lamella and haemorrhage, gill tissue condition in P1 and P2 suffered in the same destruction with moderate category, and gill tissue condition in P3 in light category. The brain tissue showed haemorrhage, congestion and necrosis. Damage to the gill and brain tissue of tilapia due to S. iniae infected.Key words : Oreochromis niloticus, Streptococcus iniae, Histopathology, Gill and Brain
IDENTIFICATION FUNGI OF COMET FISH (Carrasius auratus) BY CONVENTIONAL AND PCR (Polymerase Chain Reaction) METHOD Christin Natalia; Henni Syawal; Morina Riauwaty Siregar
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT            Fungal are a group of microbes that often cause disease in comet fish. Diseases caused by fungi are secondary infections, and can cause more than 50% death. The objective of the study was to find out the species of pathogenic fungi in comet fish and what is the prevalence of fungi that infect comet fish. The method used was survey at the location of the Ornamental Fish Shelter, Jalan Bukit Barisan, Tangkerang Timur Village, Tenayan Raya District, Pekanbaru City. The fish used were 6 - 8 cm as many as 15 tails . Conventional identification includes macroscopic inspect of fungi colonies and microscopic of fungi hyphae. Molecular identification was carried out by PCR (Polymerase Chain Reaction) technique and analyzed with Bioedit, BLASTn and MEGA applications to find out the phylogenetic tree. Research parameters include hyphae size and fungi prevalence. The results of conventional identification techniques found the 4 species of fungi, includes Aspergillus niger, Penicillium sp., Curvularia sp., Fusarium sp.. The result of molecular identification techniques found the species of fungi, includes Curvularia pseudorobusta with homolog value 93%, Trametes corrugate with homolog value 99%.,The conclusion from the identification results found two types of fungi that are pathogenic in comet fish, includes Fusarium sp. and Curvularia sp.. Fungal prevalence from 15 samples was 73,3%. Wide hyphae of fusarium sp. is 1,09 µm, and Curvularia sp. is 1,52 µm. Keywords: Fusarium sp., Curvularia sp., Carassius auratus.
Co-Authors Abdul Alif Abdul Alif Addilah, M. Fikri Adelina ' Afra Azizah Afrina Putri Afrizal Tanjung Alfian ' Alfinda, Rudi Alif, Abdul Amraini Fitri Anang Widarsa Andarini Diharmi Anderson.s, Roi Andesra, Andesra Andi Almer Farrand Noor Andre A Saragih, Andre A Andre Manusun Purba Anggriani, Rara Dewi Angraika Dirta Annisa Dwi Putri Anugrah, Rio Persal Anzila Rizki W Muharram Asmika Harnalin Simarmata Athif Amirah Elza Atika Talitha Erman Atria Martina Azizah, Afra Cerlina, Maya Chandra Chandra Chania Arnata Christin Natalia Cici Adliana Darfia, Novreta Dea Switzelin H Destriman Laoli Devy P Lumban Gaol Dian Fitria M Dian Iriani Dian Puspitowati Dicky Azwar Lubis Dinar Mey Jasika H Dwi Indah Fransiska Elfira Stefani Priska Erikson Sahala Pardamean Etri Nopilita Faira Yovanie Fandi Rahmat Farkhan, Mochammad Farrand Noor, Andi Almer Fatihaturrahma, Annisya Fauzi Ahmad Lubis Faza, Farzana Fakhira Fazira, Meisya Amanda Febi Nabila Feri Julianto Fitria Sufriyani Fransiska, Dwi Indah Fricilia Zissalwa Gaol, Devy P Lumban Ginting, Kiki Dirgantara Gunawan B S Gusriansyah, Dimas H Herdini Hafil Kurnia Tama Hasbullah Zakky Hasugian, Sampang Hotramadi Herianti Rambe Heriyanti Heriyanti, Heriyanti Herparinda Novita Husna, Hidayatul Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukstyowati Iesje Lukystiowati Ilham Fajri Pohan Iman, Khasan Nur Ipa, Adha Irma Yunita Irsya, Habib Irwan Effendi Irwan Irwan Iskandar Putra Isma Mulyani Isna Sudirman Isna Sudirman Isnaini Lidya Rizki Jampil, Tirta Herlis Jan Stepfely Simanjuntak Jaya Maruli Tamba Juniarty Manurung Juniarty Manurung Juwita Lestari Karnila, Rahman Karnila Khairia, Khairia Kiky Dirgantara Ginting Kurniawan, Ahmad Juli Kusai, Kusai Latifah Hannum Lase Lestari, Rona Luksityowati, Iesje Luskityowati, Iesje M Riswan M Zaky Ditiya Putra M. Riswan M. Yanda, M. Yanda Manurung, Juniarty Mariana Lumban Tungkup Mariska, Refi Marwati ' Maulina Sawitri Maya Cerlina Maytra Wirdona Meisya Amanda Fazira Melin Siburian Memi Martin Ilmayati Mery Sukmiwati Mesi Susanti Mochammad Farkhan Morina Riauwaty Muhamad Latiful Khobir Muhammad Akram Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Azaldin Muhammad Muhajir Muhammad Muhajir, Muhammad Mutawakkil, M. Mufid Al Mutiara Mutiara Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nasril Ismail NASTITI KUSUMORINI Nelli Karolina Sitorus Novike Zullanda Selvi, Novike Zullanda Novita, Herparinda Novreta Ersyi Darfia Nur Addini Nur Aini Nur Asiah Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nurfitri, Maya Nurhasan Nurhasan Nurhasan Nurhasan, Nurhasan Oktavianti, Mila Pakpahan, Poltak Pardamean, Erikson Sahala Pertiwi, Zuwini Poltak Pakpahan Poltak Pakpahan Purba, Andre Manusun Purba, Yoshua Dewaman Mangapul Putra, Mutlas Ade Putra, Sanjaya Mandala Putri Noviayu Salsabila Hendri Putri, Annisa Dwi Putri, Atika Putri, Mega Novia Putri, Rianti RA, Sayyidina Abdul Qabidhi Rabil Yusuf Rabil Yusuf Rahmadona, Zikra Rahmat Iman Mainil Ramadhanita, Ramadhanita Rebeka Valensia Rhino Pamungkas Riauwaty , Morina Ridho Aldiokta RIDWAN AFFANDI Rika Sinaga Rinaldi Rinaldi Rini Mastuti Rio Persal Anugrah Risman Ferdiansyah Riswan, M Riva Mutiara Rizka Hasanah Rizki Putra Rizvi, Fharisa Nabila Rona Lestari Ronal Kurniawan Roslina ' Rudi Alfinda Saberina Hasibuan Sagitha Aulya Fanny Tanjung Salsabila Hendri, Putri Noviayu Santa, Santa Santikawati, Susi Sarah Pabiola Saragih Siallagan, Romensius Anggi AW Siburian, Melin Simanjuntak, Jan Stepfely Sinaga, Tomuso Angdi Suryani Siregar, Cici YP Siregar, Desty Sahroni Siregar, Morina Riauwaty Sri Wahyuni Suci Wulandari Sudirman, Isna Syafriadiman, Syafriadiman Syafruddin Nasution Syahputra, Mohamad Ardani Syahrul Hidayat Syaieba, Mutiara Tamba, Jaya Maruli Tanjung, Yuliza Virliansyah Telaumbanua, Siti Tia Suherianti Tjipto Leksono Tjipto Leksono Tomuso Angdi Suryani Sinaga Trisla Warningsih Tungkup, Mariana Lumban Ulandari, Ulfa Unggul Fitrah Heriadi Usman M Tang Usman M Tang Usman Muhammad Tang Vekri Ramadhi Wasmen Manalu Widarsa, Anang Wijaya, Rahmat Santosa Windarti Windarti Yetti Elfina Yetti Elfina S Yoshua Demawan Mangapul Purba Yosri Yosri Yovanie, Faira Yuharmen - Yuliati Yuliati Yulyawati Yulyawati Yunita Paramitha Hasibuan Yusni Ikhwan Yusni Ikhwan Siregar Yusuf, Rabil Zebua, Ratna Dewi Zikra Rahmadona Zissalwa, Fricilia Zulaiha ' Zulkarnain Zulkarnain Zulkarnain Zulkarnain