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6. Isolation, Identification and Sensitivity Test of Staphylococcus aureus on Post Surgery Wound of Local Dogs (Canis familiaris) Darmawi Darmawi; Amalia Fatma Zahra; M. Nur Salim; Maryulia Dewi; Mahdi Abrar; Syafruddin Syafruddin; Mulyadi Adam
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4122

Abstract

This study aimed to isolate, identify, and sensitivity tested of Staphylococcus aureus from post-surgery wound of local dogs.This study was held in Microbiology Laboratorium of Veterinary Faculty of Syiah Kuala University, Banda Aceh from April to May 2016. The samples were 10 dogs in their 3rd day’s treatment after surgery.The samples were acquired using sterile swab on the wound that was planted on Nutrient Broth (NB), incubated at 37°C for 24 hours, then transferred onto Mannitol Salt Agar (MSA), incubated at 37°C for 24 hours, and after that it was identified with Gram staining, catalase test, sugar test (mannitol and glucose), Metil Red-Voges Proskauer (MR-VP) test and antibiotic sensitivity test. The result of   Gram staining showed 7 positive results which identified as coccus formed and coloured as purple. The catalase test showed 7 positive results, the sugar test (mannitol and glucose) showed 4 positive results, the MR-VP showed 4 positive on MR and all negative on VP.. In conclusion, 4 out of 10 samples showed a presence of Staphylococcus aureus on post-surgery wound of local dogs.This bacterium was sensitive on Tetracycline (50%) and also on Streptomycine and Gentamicin (100%). This bacterium resistant to  Tetracycline (50%) and Ampicillin (100%).
KEPEKAAN Pasteurella multocida YANG DIISOLASI DARI SAPI YANG BERASAL DARI KABUPATEN ACEH BARAT TERHADAP BEBERAPA ANTIBIOTIK Erni Wulandari; Faisal Jamin; Mahdi Abrar
Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i2.2941

Abstract

Penelitian ini bertujuan melihat kepekaan Pasteurella multocida yang berasal dari sapi di Rumah Potong Hewan (RPH) Meulaboh, Aceh  Barat terhadap beberapa antibiotik. Penelitian ini mengunakan 7 sampel berupa swab kerongkongan sapi yang kemudian diidentifikasi dan diperoleh 3 sampel yang teridentifikasi Pasteurella multocida,. Selanjutnya dilakukan uji kepekaan antibiotik. Dalam uji kepekaan, antibiotik yang digunakan adalah vancomisin, klindamisin, streptomisin, tetrasiklin, kloramfenikol, kanamisin, dan gentamisin. Diameter zona hambat yang terbentuk kemudian diukur dengan satuan millimeter. Hasil pengukuran menunjukkan bahwa Pasteurella multocida tidak peka terhadap vancosin, klindamisin, streptomisin, tetrasiklin, dan gentamicin kecuali kloramfenikol yang masuk dalam katagori intermediate
DETEKSI ANTIBODI SERUM AYAM KAMPUNG (Gallus domesticus) TERHADAP VIRUS NEWCASTLE DISEASE DI KOTA BANDA ACEH Darmawi -; Fakhrurrazi -; Wiliana -; Maryulia Dewi; Mahdi Abrar; Faisal Jamin; Zakiah Heryawati Manaf
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2983

Abstract

Penelitian ini bertujuan mengetahui titer antibodi pada serum ayam kampung (Gallus domesticus) terhadap virus Newcastle disease (ND). Sampel penelitian adalah serum ayam kampung yang diperoleh di beberapa pasar tradisional Banda Aceh. Parameter yang diamati dalam penelitian ini adalah titer antibodi serum ayam kampung dari uji hemaglutinasi inhibisi (HI) dengan memperhatikan endapan eritrosit. Data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa dari 120 sampel yang diambil di beberapa pasar tradisional di kota Banda Aceh hanya 15 sampel (12,5%) menunjukkan protektif sedangkan 105 sampel (87,5%) tidak protektif. Tingkat proteksi ayam terhadap virus ND di pasar tradisional di kota Banda Aceh tergolong sangat rendah.
ISOLASI DAN IDENTIFIKASI KAPANG PENYEBABDERMATOFITOSIS PADA ANJING DI KECAMATAN SYIAH KUALA BANDA ACEH Vhodzan Adzima; Faisal Jamin; Mahdi Abrar
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2920

Abstract

Penelitian ini bertujuan mengisolasi dan mengidentifikasi kapang penyebab dermatofitosis pada anjing di Kecamatan Syiah Kuala, Banda Aceh. Penelitian ini menggunakan 10 ekor anjing yang terinfeksi dermatofitosis. Bagian yang terinfeksi ringworm dibersihkan dengan alkohol 10%, lalu dikerok dengan KOH 20% dan swab. Kemudian sampel dimasukkan ke dalam 10 ml pepton water cair untuk dihomogen dan dieramkan selama 24 jam di dalam inkubator pada suhu 37° C. Biakan sampel diambil sebanyak 1 ml dimasukkan ke dalam cawan petri yang berisi 10 ml SDA yang telah ditambahkan gentamisin. Biakan kapang yang ditanam pada medium diinkubasikan pada suhu 37° C selama 5-7 hari. Untuk mengamati perkembangan dan pertumbuhan kapang yang ditanam pada slide kultur, dilakukan pewarnaan dengan laktophenol cotton blue (LCB). Kapang yang tumbuh diamati di bawah mikroskop pada pembesaran 20, 40 dan 100. Kapang diidentifikasi berdasarkan morfologi, hifa, konidia dan konidioforanya.  Hasil penelitian menunjukkan bahwa sampel kerokan kulit anjing yang diambil di Kecamatan Syiah Kuala positif terinfeksi oleh kapang golongan Tricophyton sp.
Seroprevalensi Avian influenza H5N1 pada Unggas di Kabupaten Aceh Utara Darmawi Darmawi; Darniati Darniati; Maryulia Dewi; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Erina Erina
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i2.815

Abstract

Seroprevalence of avian influenza H5N1 in birds in north aceh districtABSTRACT. Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.
Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras Darmawi Darmawi; Zakiyah Heryawati Manaf; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Erina Erina
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i1.283

Abstract

Detection on Serum Antibodies of Native Chickens to Avian influenza VirusABSTRACT. An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.
FMD RAPID DIAGNOSTIC TEST IN ACEH INDONESIA: A TECHNICAL NOTE AK, M. Daud; Abrar, Mahdi; Ferasyi, Teuku Reza
Jurnal Kedokteran Hewan Vol 18, No 4 (2024): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i4.34137

Abstract

This study aims to offer a detailed technical note thorough examination of the pioneering and effectiveness of FMD immediate diagnostic tests in Aceh, Indonesia. We discuss the key components of these tests, including their sensitivity, specificity, and practicality in resource-limited settings. Furthermore, we explore the challenges and opportunities associated with adopting these tests locally and highlight their potential impact on FMD surveillance and control efforts. The findings presented in this technical note contribute to the ongoing efforts to combat FMD in Aceh, providing valuable insights into using rapid diagnostic tests as a vital tool in disease management strategies. The results showed that the FMD rapid diagnostic test kit has limited sensitivity in detecting early clinical signs of FMD in cattle in Aceh. Therefore, Further examination with laboratory molecular detection methods is needed.
Potential of ant nest extract (Hydnophytum formicarum) for protection of testicular morphometry, epididymal functions, and sperm quality in male rats with alloxan-induced diabetes Roslizawaty, Roslizawaty; Abrar, Mahdi; Khairan, Khairan; Gholib, Gholib; Siregar, Tongku N.; Syafruddin, Syafruddin; Sutriana, Amalia; Azzahra, Putri; Aini, Hanipah
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.922

Abstract

Medicinal herbs, such as the ant nest plant (Hydnophytum formicarum), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings. For the experiment, 20 male white rats (Rattus norvegicus), aged 3–4 months and weighing 150–200 g, were equally divided into four groups. The ant nest extract was administered orally using oral gavage over 14 days. The testes, epididymis, and sperm were collected for weighing, morphometric measurements, and quality evaluation. Qualitative testing of phytochemical compounds indicated the presence of flavonoids, tannins, steroids, terpenoids, and phenolic compounds in the plants. The results revealed the protective effects of ant nest extract against the adverse fertility effects induced by alloxan and a high-fat diet, as observed in testicular weight (p=0.045), epididymal weight (p=0.041), and sperm quality (p>0.05).
DETECTION OF MULTIDRUG-RESISTANT Klebsiella pneumoniae IN THE UPPER RESPIRATORY TRACT OF BROILERS AND LAYER CHICKENS FROM COMMUNITY FARM IN ACEH BESAR, INDONESIA Darniati, Darniati; Erina, Erina; Abrar, Mahdi; Zainuddin, Zainuddin; AK, M Daud
Jurnal Kedokteran Hewan Vol 19, No 2 (2025): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i2.45844

Abstract

This study aimed to investigate the presence of multidrug-resistant (MDR) Klebsiella pneumoniae in upper respiratory tract of broiler chickensand laying hens from community farms in Aceh Besar. Tracheal swab samples were collected and cultured on eosin methylene blue agar for bacterialisolation. Suspected isolates were identified through biochemical testing and confirmed via PCR targeting the rpoB gene. Identified isolates weretested for antibiotic susceptibility against ciprofloxacin, tetracycline, gentamicin, erythromycin, and chloramphenicol, using the disc diffusionmethod. The results revealed K. pneumoniae in 6% of broiler and 14% of laying hens, with widespread multidrug resistance. All isolates wereresistant to erythromycin (100%), gentamicin (100%), tetracycline (85%), ciprofloxacin (40%), and chloramphenicol (15%). These findingsconcluded that there has been the spread of MDR of K. pneumoniae in broiler chickens and laying hens within Aceh Besars community farms inAceh Besar, Indonesia.
21. -Amylase and -Glucosidase Inhibitors from Plant Extracts Daud AK, M; Juliani, Juliani; Sugito, Sugito; Abrar, Mahdi
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.13819

Abstract

About 80% of diabetic people have come from developing countries. Indonesia is seventh of diabetics. Diabetes is a disease as a result of metabolic disorder that caused by lack of insulin secretion and/or insulin do not work properly, so that the sugar highly accumulated in blood (characterized by hyperglycemia). Normally, range of blood sugar is 70 - 110 mg / dL before meals and less than 140 mg / dL after meals. One therapeutic approach to control blood sugar level is to inhibit the activity of starch hydrolysis enzymes, namely -amylase and -glucosidase. The active compounds of plant extracts have a unique and diverse structure compared to synthetic ones. This characteristics are important because the inhibition of enzyme activity by active compounds from plant extracts is known to make the formation of chemical bonds (affinity) between the active compounds and enzymes. A number of active compounds from plant extracts such as tannins, flavonoids, polysaccharides, saponins, terpenoids are known to inhibit the activity of -amylase and -glucosidase. In this study, we discuss some active compounds that inhibit -amylase and -glucosidase enzyme from plant extracts.
Co-Authors - Fakhrurrazi . Darmawi . Darniati . Nurliana Abd. Rasyid Syamsuri Abdullah Hamzah Afriyani Afriyani Aini, Hanipah AK, M Daud Amalia Fatma Zahra Amalia Sutriana Aminuyati Andi Novita Arman Sayuti Azzahra, Putri Balqis Alrasyid Suyoto Bambang Pontjo Priosoeryanto Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan budianto panjaitan Chairul Saputra Siregar chiara giri puspa cut nila thasmi Darmawi - Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Darmawi Daud AK, M Dwinna Aliza Dwinna Aliza Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina erma yusintha ramdhania Erni Wulandari Etriwati E Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fadli A. Gani Faisal Jamin Faisal Jamin Faisal Jamin Fakhrurrazi - Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Farida Athaillah Gholib Gholib Gholib Gholib, Gholib Hafizuddin Hafizuddin Hamny Sofyan Hanni Aninaidu Helmi, T. Zahrial Herrialfian . I wayan Teguh Wibawan Ismail Ismail ismail ismail ismail Juli Melia Juli Melia Juliani Juliani Juliani Khairan Khairan Khairiah Khairiah Khairul Rizal Liza Setia Joni M Aman Yaman M Daud AK M Nur Salim M. Chairul Amri M. Iqbal Pratama Sekedang M. Nur Salim Maryulia Dewi Maryulia Dewi Maryulia Dewi Maryulia Dewi Masda Admi Mega Cempaka Putri Mirnawati Soedarwanto Mirnawati Soedarwanto Mirnawati Soedarwanto Muhammad Jalaluddin Muhammad Zulfan Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Muttaqien Bakri Nanda Yulian Syah Nellita Meutia Nellita Meutia, Nellita Nurdiani Muliana Sitakar NURLIANA NURLIANA NURLIANA NURLIANA Rastina Rastina Rastina Rastina Razali Daud Razali Daud Razali Razali Rezi Maghfira Rivaldi Luthfi Rizal, Khairul roslizawaty roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Sari, Wahyu Eka Siregar, Tongku N. Sri Wahyuni Sugito - Sugito Sugito Sugito Sugito Suryani Suryani Syafruddin - Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin T Reza Ferasyi T Zahrial Helmi T. Armansyah T. Fadrial Karmil T. Fadrial Karmil Teuku Reza Ferasyi Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Ummu Balqis Vhodzan Adzima Vinky Claudya Fransiska Wiliana - Winaruddin Winaruddin Yanrivina S.V.S Yudha Fahrimal Yulina Rahmi Zainuddin, Zainuddin Zakiah Heryawati Manaf Zakiah Heryawati Manaf Zakiah Heryawaty Manaf Zakiyah Heryawati Manaf Zuhrawati Zuhrawati Zuhrawati Zuhrawati, Zuhrawati Zuhrawaty Zuhrawaty