Kadek Karang Agustina
Department Of Public Health, Faculty Of Veterinary Medicine, Udayana University, Denpasar, Indonesia

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THE KNOWLEDGE AND ATTITUDES OF THE COMMUNITY IN SIBANGKAJA VILLAGE, BADUNG, REGARDING THE RABIES INCIDENT: Pengetahuan dan Sikap Masyarakat Desa Sibangkaja, Badung terkait Kejadian Rabies I Made Subrata; Putu Erma Pradnyani; Sang Gede Purnama; Ni Wayan Arya Utami; Janice Girardi; Kadek Karang Agustina; Ida Bagus Ngurah Swacita; Hendra Irawan; Kate Druhan
Jurnal Berkala Epidemiologi Vol. 11 No. 2 (2023): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V11I22023.128-134

Abstract

Background: The challenges in overcoming the rabies epidemic include the public's lack of knowledge and attitudes regarding the control and first aid in dog bites. Sibangkaja is one of the villages in Badung Regency, which is an endemic area for rabies. Purpose: This study aimed to describe the knowledge, attitudes, and factors influencing the behavior of the residents of Sibangkaja Village, Badung, regarding rabies. Methods: This descriptive observational study was carried out using a cross-sectional design. The samples were 250 residents of Sibangkaja Village, taken by convenience sampling. Data were collected from February to March 2020 through interviews using the digital questionnaire. The variables were knowledge and community attitudes toward rabies vaccination in the village. Results: The results showed that the respondents' knowledge was good, but 34% did not know the symptoms of rabies. It was discovered that attitudes toward rabies prevention and management are significantly more in favor of vaccination than eliminating dogs. Furthermore, there is an association between knowledge and dog ownership on people's attitudes (p-value=0.01). Conclusion: Information and education about the symptoms of rabies is needed for residents to exercise caution and pay more attention to themselves and the surrounding dogs.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis I Made Kardena; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; Ida Bagus Windia Adnyana; I Made Sukada; Kadek Karang Agustina; Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p02

Abstract

Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
Detection of antibacterial activity in chicken meat, eggs, drinking water, animal feed and sewage waste in Tabanan, Bali Setiabudy, Marta; Indraningrat, Anak Agung Gede; Suryanditha, Putu Arya; Budayanti, Ni Nyoman Sri; Yanti, Ni Komang Semara; Adhiputra, I Ketut Agus Indra; Widowati, I Gusti Ayu Rai; Agustina, Kadek Karang
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 1 (2023): Available online : June 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i1.51

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Background: The use of antibiotics that are not in accordance with the indications, doses, and duration can trigger resistance and there is concern that it might leave antibiotic residues in the processed product. Aim of this study was to detect the antibacterial activity of livestock products, namely chicken meat and eggs and the surrounding environment such as drinking water, animal feed and waste disposal. This study was a preliminary study before the establishment of antibiotic wise village, One Health approach for antimicrobial stewardship program. Methods: This study was a descriptive study with a cross sectional design to determine antibacterial activity, particularly tetracycline in livestock products and the environment. The research samples were taken from 5 groups of farmers in one of the villages in Tabanan, Bali. Bioassay method based on the Kirby Bauer method was used in this study. Results: From a total of 44 samples, 6 samples showed weak antibiotic tetracycline activity (13.6%), namely in waste disposal (20%) and animal feed (40%). Antibiotic contamination was likely to occur because the animal feed used in this group contains antibiotics with or without the knowledge of the farmers themselves. Disposal waste came from livestock manure that ate the feed or from animal feed that was scattered around the cage. Conclusion: Samples of livestock meat and eggs did not show antibacterial activity. There were samples that have antibiotic activity but weak and inconsistent, namely in samples of waste disposal and animal feed. This condition cannot necessarily be concluded as antibiotic abuse in livestock however it can be the basis for the importance of providing education regarding antimicrobial resistance. Unless there was indication, antibiotics should not be given to livestock on a daily basis.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
The Seroprevalence of Toxoplasma gondii in Cats at the House of Maternal Women with Toxoplasmosis in Badung, Indonesia Subrata, Made; Astawa, Nyoman Mantik; Suryadi, Nyoman Tigeh; Purnama, Sang Gede; Agustina, Kadek Karang; Harjana, Ngakan Putu Anom; Damriyasa, Made
Kesmas Vol. 16, No. 4
Publisher : UI Scholars Hub

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Abstract

Toxoplasmosis is a zoonotic disease caused by infection with the parasite called Toxoplasma gondii (T. gondii). The health and social impacts of the infectionare enormous, including miscarriage, hydrocephalus, blindness, and mental retardation. The occurrence of toxoplasmosis in maternal women cannot be se parated from cats around their houses. This study aimed to determine the seroprevalence of the parasite in cats found in the human carriers residences and identify the risk factors of toxoplasmosis in maternal women in Badung District, Bali Province, Indonesia. A total of 80 cat serum samples were obtained from two residential groups, 40 from the housing where the maternal women were infected and another 40 from where there were no identified sufferers of the disease. All the samples were examined using the enzyme-linked immunosorbent assay (ELISA) method to detect the presence of antibodies T. gondiiin feralcat serum. The results showed that 47.5% of the examined subjects had the said antibodies. As much as 65% came from housing with cases of toxoplasmosisin maternal mothers, and 30% came from residences with none. The presence of feral cats is a major risk factor for the transmission of T. gondiito humans.
IMPLEMENTATION OF ANIMAL WELFARE ON PIG FARMS IN SEBATU AND TARO VILLAGE, TEGALLALANG DISTRICT, GIANYAR BALI Ni Putu Juni Ratna Dewi; Kadek Karang Agustina; Romy Muhammad Dary Mufa
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p28

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The increasing demand for pork has led some farmers to allegedly ignore animal welfare principles, focusing more on achieving high production targets. The purpose of this study was to determine the application of animal welfare standards on pig farms in Sebatu and Taro Village, and see the differences between the two villages. The method used was a survey of farmers, namely 25 farmers in each of the two villages. The data obtained were analysed descriptively and comparatively. The results showed that pig farmers in Sebatu and Taro villages have implemented animal welfare very well 54% and 46%. Comparative analysis found no difference in the application of animal welfare between the sampling villages. It can be concluded that the principles of animal welfare have been fulfilled for pigs in Sebatu and Taro Village. It is recommended that farmers reduce the use of battery cages, and provide enrichment facilities for pigs to express their natural habits.
Cover and Table of Content of BVU Vol. 16 No. 1 February 2024 Kadek Karang Agustina
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

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Abstract

Cover and Table of Content of BVU Vol. 16 No. 1 February 2024
SYSTEMATIC REVIEW: ZOONOSIS ASSOCIATED WITH MOUSE AND RAT Kadek Karang Agustina
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.136

Abstract

Mouse and rat borne zoonotic diseases refer to illnesses that can be transmitted from rodents such as mice and rats to humans. These animals can carry various pathogens, including bacteria, viruses, and parasites, which can cause diseases in humans through direct contact, inhalation of airborne particles, consumption of contaminated food or water, or via vectors such as ticks and fleas. Here are some common mouse and rat zoonotic diseases: Leptospirosis, Hantavirus Pulmonary Syndrome (HPS), Lymphocytic Choriomeningitis (LCM), Rat-Bite Fever (RBF), Salmonellosis dan Pes. Leptospirosis: This bacterial infection is caused by Leptospira bacteria found in the urine of infected rodents. Humans can contract leptospirosis through contact with contaminated water, soil, or food. Symptoms can range from mild flu-like illness to severe complications involving the kidneys, liver, and other organs. Hantavirus Pulmonary Syndrome (HPS): Hantaviruses are transmitted to humans through contact with rodent urine, droppings, or saliva, primarily from deer mice. Inhalation of aerosolized virus particles is the most common route of transmission. HPS can lead to severe respiratory illness, with symptoms including fever, muscle aches, coughing, and potentially fatal pulmonary edema. Lymphocytic Choriomeningitis (LCM): LCM is caused by the Lymphocytic Choriomeningitis Virus (LCMV), which is carried by house mice. Humans can become infected through exposure to rodent urine, droppings, saliva, or nesting materials. LCM can cause flu-like symptoms initially and may progress to more severe neurological complications, including meningitis and encephalitis. Rat-Bite Fever (RBF): This bacterial infection is caused by Streptobacillus moniliformis (commonly associated with rat bites) or Spirillum minus (associated with exposure to rat feces or urine). RBF can result from a bite or scratch from an infected rodent or handling of contaminated materials. Symptoms include fever, rash, joint pain, and in severe cases, endocarditis or meningitis. Salmonellosis: Salmonella bacteria can be carried by rodents, particularly in their feces. Humans can contract salmonellosis through the ingestion of contaminated food or water, as well as through direct contact with rodents or their habitats. Symptoms typically include diarrhea, abdominal cramps, fever, and vomiting. Pes: While commonly associated with fleas that infest rodents like rats, plague bacteria (Yersinia pestis) can also be transmitted directly through contact with infected rodents or their bodily fluids. Plague can manifest as bubonic (swollen lymph nodes), septicemic, or pneumonic forms, with symptoms ranging from fever and chills to respiratory distress and organ failure. Preventing mouse and rat zoonotic diseases involves maintaining good hygiene practices, such as proper food storage, waste disposal, and rodent-proofing buildings. Additionally, avoiding contact with wild rodents and seeking prompt medical attention if exposed to rodents or experiencing symptoms of illness are crucial preventive measures.
THE IMPLEMENTATION OF ANIMAL WELFARE STANDARDS ON PIG FARMS IN THE HIGHLANDS AND LOWLAND AREA IN GIANYAR BALI I Nyoman Bagus Tri Aribawa; Kadek Karang Agustina; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p29

Abstract

Animal welfare is everything related to the physical and mental state of animals in accordance with the standard of natural animal behaviour that needs to be implemented to protect animals from inappropriate treatment by humans. This study aims to determine the description of the application of animal welfare on pig farms in the highlands and lowlands in Gianyar Regency. The research design was observational by interviewing 20 farmers in each of Puhu Village (highland) and Pering Village (lowland) conducted in January 2024 using an integrated questionnaire. The data obtained were then analysed descriptively qualitatively and presented in tabular form. The Mann Whitney test was conducted to determine the difference between the application of animal welfare standards in highland and lowland areas. The results showed that the application of animal welfare on pig farms in Gianyar was in the very good category 30%, good category 65%, and fair category 5%. While the results of the comparison test found a difference in the fulfilment of the principle of free expression of the natural behaviour of pigs. It can be concluded there was a difference between the application of animal welfare standards on pig farms in the highlands and lowlands. It is recommended that pig farmers in Pering village provide enrichment facilities for pigs to express their natural behaviour.
IMPLEMENTATION OF ANIMAL WELFARE PRACTICES AMONG LAYER FARMERS IN PUHU AND PERING VILLAGES IN GIANYAR REGENCY Ngakan Nyoman Cahaya Adi; Kadek Karang Agustina; I Made Sukada
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p13

Abstract

Laying hens are chickens that are raised with the aim of producing eggs with high productivity. In order to maximize the productivity of laying hens, many farmers use a battery cage system. Battery cages actually implement poor welfare due to extreme confinement and restrictions on chicken behavior. This study aims to determine the application of animal welfare on layer farms in two villages with different altitudes. Pering village located in lowland and Puhu village located in highland. The data collection method used a questionnaire by conducting interviews with layer farmers. Data were analysed descriptive quantitatively and Mann Whitney comparison test was conducted to compare data between sampling villages. The results of the analysis showed that there were significant differences in the application of the concept of freedom from discomfort and the application of the concept of freedom to express natural behavior. It can be concluded that there is a significant difference in the application of the five freedom principles between the two villages. The researcher suggested to the farmers in Puhu village to start trying the free cage method which in principle is more prosperous for the chickens.
Co-Authors Adhiputra, I Ketut Agus Indra Adiwinata, Putu Diva Ady Fendriyanto, Ady Ahmad Harits Fitra Mauladi Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Andriawino Berdionis Sanam Anne Dawdoya Arinata, I Ketut Teguh Astrid Laboure Parahita Cesarina Pascalia dos Santos Alegria Daniella Everetta Desak Wiga Puspita Dewi Desy Ariani Ferdianti, Desy Ariani Dewa Akbar Maulana Dewa Made Dwi Parwata Dewi Titis Angganingrum Dimas Indra Dwi Purnama Dina Elviana DWI SURYANTO Elis Mandari Emerensia Patryconsitha Aman Emia Pepa Yosa Br Ginting Erma Pradnyani, Putu Gede Widiantara Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana Gusti Made Widyantara Handayani, Ni Made Harjana, Ngakan Putu Anom Hendra Irawan I Gede Mahardika I Gede Raka Mas Tanaya I Gusti Agung Ayu Suartini I GUSTI AYU FITRI DIASTARI I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Kadek Swastika I Ketut Berata I Ketut Suada I Ketut Suada I Ketut Suada I Ketut Tomy Caesar Ramanda I Made Damriyasa I Made Dwinata I Made Gede Asta Ginawan I Made Gede Wijaya Kusuma I Made Kardena I Made Ricky Dwi Cahya I Made Subrata I Made Subrata I Made Subrata I Made Sukada I MAde Sukada I Made Sukada I Made Sukada I Made Sukada I Nengah Kerta Besung I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I Nyoman Bagus Tri Aribawa I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Agus Kertawirawan, I Putu Agus I Putu Ardiawan I Putu Sampurna I Wayan Batan I Wayan Masa Tenaya I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Polos I Wayan Suardana I Wayan Suardana I Wayan Sudarmayasa I Wayan Sudira I Wayan Wahyudi, I Wayan I Wayan Wirata I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Ayu Putu Aselya Mardyawati Ida Bagus Dimas Kusumadarma Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Krisna Pradnyadana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Ngurah Swacita Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Indraningrat, Anak Agung Gede Janice Girardi Janice Girardi Joanita Auxilia de Sa Pereira Julita Dewitri Merthayasa Kate Druhan Ketut ELI Supartika KETUT SUADA Ketut Tono Pasek Gelgel Luh Made Sudimartini Luh Putu Pradnya Swari M.D. Rudyanto Made Damriyasa, Made Made Suma Anthara MAS DJOKO RUDYANTO Mufa, Romy Muhammad Dary MURNI SARI Natalia Natalia Ngakan Nyoman Cahaya Adi Ngakan Putu Anom Harjana Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Ketut Suwiti Ni Komang Dyah Purnamasari Ni Luh Gede Sudaryati Ni Made Ayudiningsih Astiti Sudewi Ni Nyoman Sri Budayanti Ni Putu Juni Ratna Dewi Ni Putu Tessa Arsaning Rahayu Ni Wayan Arya Utami Ni Wayan Arya Utami Ni Wayan Arya Utami, Ni Wayan Arya Nyoman Sadra Dharmawan Nyoman Sadra Dharmawan Nyoman Suartha Nyoman Tigeh Suryadi Pande Putu Januraga Prabarini Hanum Sari Putra, I Made Maha Putri Destarani Putu Agus Trisna Kusuma Antara Putu Arya Suryanditha Putu Ayu Sisyawati Putriningsih Putu Henrywaesa Sudipa Putu Sasmitha Devi Cahyani Putu Velyniawati Ribka Listia Br Tarigan, Ribka Listia Romy Muhammad Dary Mufa Romy Muhammad Dary Mufa Sabelina Fitriani Samuel Mura sang gede purnama Sang Gede Purnama Sang Gede Purnama Segantara, Luh Gede Victoria Setiabudy, Marta Sonia Dewi Citra Sembiring Sosiawan, I Gede Arya Mas Sri Kayati Widyastuti Sudiartawan, I Putu Sukada, Made Sukardika, Ketut Elok Sumarya, I Made Suputra, Gusde Wahyu Krisna Suryadi, Nyoman Tigeh Suwartama, Beny T. Sari Nindia Thea Sarassati, Thea Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjokorda Sari Nindhia TRI KOMALA SARI Uli Rehlitna Sembiring, Uli Rehlitna Vinensia Ghona Gani Widayantari, Anak Agung Ayu Sauca Sunia Widowati, I Gusti Ayu Rai - Wilson Lois, Wilson Wirata, Ketut Yanti, Ni Komang Semara