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All Journal JURNAL KIMIA SAINS DAN APLIKASI Indonesian Journal of Urology Jurnal Kesehatan Andalas Jurnal Riset Kimia Biology, Medicine, & Natural Product Chemistry Jurnal Biologi Tropis Majalah Kedokteran Andalas Andalas obstetrics and gynecology journal Reswara: Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian Pendidikan, Psikologi Dan Kesehatan (J-P3K) Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Narra J Cermin Dunia Kedokteran Jurnal Ilmu Kesehatan Indonesia (JIKSI) Jurnal Riset Ilmiah Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Dessy Arisanty
Departemen Biokimia Fakultas Kedokteran Universitas Andalas, Padang 25163, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Distribusi Mutasi Asp614Gly pada Spike Protein SARS-CoV-2 di Sumatera Barat Agustian, Dede Rahman; Putra, Andani Eka; Arisanty, Dessy
Majalah Kedokteran Andalas Vol. 47 No. 2 (2024): MKA April 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i2.p163-172.2024

Abstract

Objective: This study aims to map the distribution of the Aspartate 614 to Glycine (Asp614Gly) mutation in the spike protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) circulating in West Sumatra, to understand the extent of its spread within the local population. Methods: RNA samples were taken from 98 confirmed COVID-19 cases across various area in West Sumatra. Genetic amplification and next-generation sequencing (NGS) were performed to identify the presence of the Asp614Gly mutation. The frequency of mutations was analyzed using bioinformatics software. Results: Out of the 98 samples analyzed, approximately 97% exhibited the Asp614Gly mutation. The geographic distribution of this mutation showed significant variation among the districts of West Sumatra, with the highest prevalence in urban areas. Conclusion: These findings indicate that the Asp614Gly mutation is highly prevalent in West Sumatra. Associated with increased virus transmissibility, this mutation dominates the circulating virus strains in the region, highlighting the need for ongoing genetic surveillance to guide public health interventions and vaccination strategies.
Education on Exposure and Health Risks of Forever Chemicals (PFAS) for Housewives and Adolescent Girls Ilmiawati, Cimi; Silvia, Roza; Arisanty, Dessy; Usman, Elly; Masrul; Amir, Arni; Hasmiwati; Reza, Mohamad; Ariadapa Rahadi, Didan; Fauzan, Rezky; Abi Ghofari Siregar, Muhammad; Olivia, Ivonne; Dwiharyani, Yuli; Ilham, Muhammad
Warta Pengabdian Andalas Vol 33 No 1 (2026)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.33.1.28-38.2026

Abstract

Our community service activity aimed to increase knowledge and awareness about the dangers of per- and polyfluoroalkyl substances (PFAS), known as “forever chemicals,” which are commonly found in household products, food packaging, and cosmetics. Long-term exposure to PFAS has been associated with numerous serious health effects, including an increased risk of several cancers, disruption of hormone homeostasis, liver toxicity, impaired immune function, and adverse reproductive and developmental outcomes.  The participants included housewives and adolescent girls (n = 20) who attended Padang Pasir Community Health Center, East Padang, Padang on 6 September 2025. An interactive educational session was conducted using a pre-test–post-test design to evaluate changes in knowledge and awareness. Analysis of participants who completed both assessments showed an improvement in knowledge following the intervention, with the greatest increases observed in understanding of healthy cooking utensils (50%) and the presence of persistent chemicals in cosmetics (50%). However, evaluation of awareness levels revealed only a minimal increase in median scores (from 57 to 60), which was not statistically significant (p = 0.221). We conclude that a focused educational session is effective in improving participants’ cognitive knowledge, but a more comprehensive and sustained approach is needed to promote meaningful behavioral change and deeper awareness, particularly since practicality and convenience remain major considerations when choosing household products, food packaging, and cosmetics.
ANALYSIS OF L-Asparaginase II ENZYME ACTIVITY FROM NATIVE AND RECOMBINANT Escherichia coli DH10B FOR THE APPLICATION OF ACUTE LYMPHOBLASTIC LEUKAEMIA THERAPY Keysha Putri Febiona; Dian Pertiwi; Arni Amir; Lily Syukriani; Dessy Arisanty; Almurdi Almurdi; Lisana Shiddiqin Aliya; Jamsari Jamsari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Acute Lymphoblastic Leukemia (ALL) is a blood cancer that occurs due to abnormal proliferation of lymphoid progenitor cells. L-asparaginase II is an enzyme used to treat this disease by inducing apoptosis in cancer cells encoded by the AnsB gene. Previous studies have shown complications and toxicity in commercial L-asparaginase, necessitating exploration of L-asparaginase sources. Serratia plymuthica UBCF_13 is one of the microorganisms that encodes the AnsB gene. This study consisted of inoculation of native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB, molecular validation, enzyme activity testing using direct nesslerisation method, and analysis of extracellular and intracellular protein profiles using SDS-PAGE. Enzyme activity and protein profiles were compared with S. plymuthica UBCF_13, and Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction was performed on each bacteria as a comparison. The results showed the highest L-asparaginase II enzyme activity in E. coli DH10B pET28a(+)_AnsB induced with IPTG with an average enzyme activity of 0.392 ± 0.017 U/mL. Protein profile analysis using SDS-PAGE supported this finding, showing a thicker 36.5 kDa protein band compared to other bacteria. Statistical testing using One Way ANOVA showed a significant difference in L-asparaginase II enzyme activity in native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB. It can be concluded that there was an increase in activity in recombinant E. coli DH10B compared to its native.
ANALYSIS OF L-Asparaginase II ENZYME ACTIVITY FROM NATIVE AND RECOMBINANT Escherichia coli DH10B FOR THE APPLICATION OF ACUTE LYMPHOBLASTIC LEUKAEMIA THERAPY Keysha Putri Febiona; Dian Pertiwi; Arni Amir; Lily Syukriani; Dessy Arisanty; Almurdi Almurdi; Lisana Shiddiqin Aliya; Jamsari Jamsari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Acute Lymphoblastic Leukemia (ALL) is a blood cancer that occurs due to abnormal proliferation of lymphoid progenitor cells. L-asparaginase II is an enzyme used to treat this disease by inducing apoptosis in cancer cells encoded by the AnsB gene. Previous studies have shown complications and toxicity in commercial L-asparaginase, necessitating exploration of L-asparaginase sources. Serratia plymuthica UBCF_13 is one of the microorganisms that encodes the AnsB gene. This study consisted of inoculation of native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB, molecular validation, enzyme activity testing using direct nesslerisation method, and analysis of extracellular and intracellular protein profiles using SDS-PAGE. Enzyme activity and protein profiles were compared with S. plymuthica UBCF_13, and Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction was performed on each bacteria as a comparison. The results showed the highest L-asparaginase II enzyme activity in E. coli DH10B pET28a(+)_AnsB induced with IPTG with an average enzyme activity of 0.392 ± 0.017 U/mL. Protein profile analysis using SDS-PAGE supported this finding, showing a thicker 36.5 kDa protein band compared to other bacteria. Statistical testing using One Way ANOVA showed a significant difference in L-asparaginase II enzyme activity in native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB. It can be concluded that there was an increase in activity in recombinant E. coli DH10B compared to its native.
PROFILE OF PATIENTS WITH UROLITHIASIS IN RSUP DR. M. DJAMIL IN 2022 Hanif, Pandu Muhammad; Zulfiqar, Yevri; Windasari, Noverika; Alvarino; Rustam, Erlina; Arisanty, Dessy
Indonesian Journal of Urology Vol 33 No 2 (2026)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v33i2.972

Abstract

Introduction: Urolithiasis or urinary stone refers to the stone formation along the urinary tract, from the kidney to the urethra. Urolithiasis prevalence is known to differ across continents and across the country. A study in Indonesia in 2013 reported a national prevalence of 0.6% and a regional prevalence 0.4% in West Sumatra. Objective: This study aimed to determine patients characteristics and provide an overview of stone distribution among patients with urolithiasis at RSUP Dr. M. Djamil Padang. Material & Methods: This study was a descriptive observational research with a cross-sectional design using medical record data of patients with urolithiasis in RSUP Dr. M. Djamil Padang during January - July 2022. A total sampling method was used to collect data from 273 patients. Results: This research showed that most of the patients were males (58.24%) and aged between 51 - 60 years old (34.43%). The stones found were more commonly located in the upper urinary tract, comprising the kidney (73.26%) and ureter (14.65%). Conclusion: This study concluded that male was more likely to develop urolithiasis than female, and so did people aged 51 - 60 years old compared to the other age range. The stone’s location was more likely to occur in the kidney. Keywords: Urolithiasis, patients characteristics, stone’s location.
Evaluation of Antioxidant and Cytotoxicity Activities of Mimosa pudica Linn. Leaf Extract Combined with Silver Nanoparticles (AgNPs) Against Human Colorectal Cancer HCT116 Cell Rahmad Fajral Ilhami; Dian Pertiwi; Dessy Arisanty; Yanti Rahayu
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1129-1138

Abstract

Colorectal cancer is one of the leading causes of cancer-related morbidity and mortality worldwide, with oxidative stress playing a pivotal role in colorectal carcinogenesis. The limitations of conventional therapies, including systemic toxicity and drug resistance, have driven the exploration of alternative therapeutic agents derived from natural products. Mimosa pudica Linn. leaves are known to contain bioactive compounds with antioxidant and anticancer properties; however, their clinical application is limited by poor biopharmaceutical characteristics. The combination of Mimosa pudica leaf extract with silver nanoparticles (AgNPs) is expected to enhance its biological efficacy through nanotechnology-based drug delivery systems. This study was conducted as an in vitro experimental investigation. Mimosa pudica Linn. leaf extract was prepared using an extraction method and subsequently combined with silver nanoparticles. Antioxidant activity was evaluated using the DPPH assay, with ascorbic acid serving as a positive control. Cytotoxic activity against the human colorectal cancer cell line HCT116 was assessed using the MTT assay to determine cell viability and IC50 values. Statistical analyses were performed to evaluate differences among treatment groups. The results demonstrated that Mimosa pudica Linn. leaf extract exhibited significant antioxidant activity, although it was lower than that of ascorbic acid. In cytotoxicity assays, the leaf extract reduced HCT116 cell viability in a dose-dependent manner. Notably, the combination of Mimosa pudica leaf extract with silver nanoparticles produced a more pronounced cytotoxic effect compared to the extract alone, as indicated by a greater reduction in cell viability and lower IC50 values. In conclusion, Mimosa pudica Linn. leaf extract possesses antioxidant and cytotoxic activities against HCT116 colorectal cancer cells. Its combination with silver nanoparticles enhances cytotoxic potency, highlighting its potential as a nanomedicine-based anticancer candidate for colorectal cancer therapy.
The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines Dessy Arisanty; Zolkapli Eshak; Fatma Sri Wahyuni; Afifatul Achyar; Salsabila Putri Khairani
Jurnal Kimia Sains dan Aplikasi Vol 29, No 1 (2026): Volume 29 Issue 1 Year 2026
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.29.1.58-63

Abstract

Breast cancer remains one of the leading causes of death in women worldwide, with high incidence and mortality rates. The abnormal expression of the small non-coding RNA, microRNA-10b (miR-10b), has been associated with breast cancer progression. miR-10b is involved in various biological processes, including proliferation, metastasis, and drug resistance. However, the underlying mechanism by which miR-10b contributes to breast cancer progression remains unclear. This study aimed to investigate the effect of miR-10b on the expression of HOXD10 and PI3K genes, as well as its impact on cell viability and proliferation in breast cancer cells. In this study, T47D breast cancer cells were transfected with mimic miR-10b using the FANA-oligo transfection method at concentrations of 1, 2.5, and 5 µM. Cell viability was assessed using the MTT assay, while HOXD10 and PI3K gene expression levels were measured using RT-qPCR and ELISA. Data were analysed using paired t-tests with SPSS, and results were considered statistically significant at p < 0.05. The results showed that mimic miR-10b induced a dose-dependent increase in cell proliferation. Notably, only HOXD10 gene expression was significantly affected by the transfection, while PI3K gene expression remained unchanged. Cells transfected with 5 µM miR-10b mimic exhibited the highest proliferation rate compared to non-transfected controls (p < 0.05). In conclusion, miR-10b promotes breast cancer cell proliferation by downregulating HOXD10 expression, independent of PI3K, suggesting it is a potential target for further investigation.
Program Edukasi Hidrasi Anak Sekolah Full Day di SDIT Alam Ar-Royyan Kota Padang Aulia Sepriadina Larasati; Ulya Uti Fasrini; Noverika Windasari; Desmawati Desmawati; Linosefa Linosefa; Dessy Arisanty
Reswara: Jurnal Pengabdian Kepada Masyarakat Vol 7, No 1 (2026)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/rjpkm.v7i1.7657

Abstract

Konsumsi air yang cukup sesuai rekomendasi berperan penting bagi fungsi kognitif dan kesehatan anak. Akan tetapi, masih dijumpai anak sekolah dasar yang belum memahami manfaat mencukupi kebutuhan air tubuh. Program pengabdian ini bertujuan menilai sekaligus meningkatkan pengetahuan tentang hidrasi pada siswa kelas 4 dan 5 di sekolah dasar full day berbasis alam. Mitra program adalah SDIT Alam Ar-Royyan di Kota Padang. Metode yang digunakan berupa edukasi interaktif selama tiga sesi selama tiga minggu. Setiap sesi diawali pengarahan dan pre-test, dilanjutkan penyampaian materi melalui media audio visual, permainan, serta tanya jawab. Post-test dan penguatan materi dilaksanakan dua hari setelahnya. Instrumen penilaian menggunakan kuesioner cetak dan dianalisis secara deskriptif. Sebanyak dua puluh tiga siswa mengikuti pertemuan pertama, dengan tujuh belas siswa menyelesaikan seluruh rangkaian program. Hasil menunjukkan peningkatan pengetahuan yang bermakna yaitu sebesar 96,1% siswa mengalami kenaikan skor pengetahuan dan 76,5% berpengetahuan “baik”. Ukuran kelompok yang kecil mendukung interaksi, fokus, dan umpan balik yang lebih personal. Hambatan utama adalah penjadwalan dan ketidakhadiran sebagian siswa. Penetapan jadwal melalui nota kesepahaman dengan bagian kurikulum, pelibatan orang tua dan guru melalui diskusi kelompok terarah, serta pemantauan kebiasaan minum harian di kelas diperlukan untuk keberlanjutan program.
Design and Optimization of a CD4 Primer for Quantitative Real-Time PCR (qRT-PCR) Puji Oktavia; Andani Eka Putra; Gestina Aliska; Zelly Dia Rofinda; Dwitya Elvira; Dessy Arisanty
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12832

Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) is being explored as an affordable alternative to flow cytometry for monitoring CD4+ T lymphocytes in HIV-infected patients, but detailed design and validation of CD4-specific primers are rarely reported. To design a CD4-specific primer-probe set for qRT-PCR, assess its in silico specificity, and determine the optimal annealing temperature. A forward primer, reverse primer, and hydrolysis probe targeting CD4 transcript were designed from published CD4 gene sequences. Specificity was evaluated using NCBI Primer-BLAST against the Human mRNA RefSeq database. Optimal annealing temperature was determined using an 8-point thermal gradient (54.0-66.0°C) on a Bio-Rad CFX96 system with SensiFAST™ Probe No-ROX chemistry, comparing Cq values across the gradient. Results: Primer-BLAST confirmed specificity of the forward (20nt, Tm 57.42°C, GC 50.00%) and reverse (24nt, Tm 57.69°C, GC 41.67%) primers, with low self-complementarity (3.00 and 2.00) and no 3' self-complementarity. Cq values were tightly clustered (23.25-23.76) across the gradient; with the lowest Cq (23.25) at 54.8°C. The CD4-specific primer-probe set showed confirmed specificity and robust tolerance across a wide annealing temperature range, with an optimum of 54.8°C, supporting its use as a foundational tool for CD4+ T lymphocyte monitoring where flow cytometry is inaccessible.
Co-Authors Aaliyya, Zaki S. Abdah M.D. Akim Abi Ghofari Siregar, Muhammad Afifatul Achyar Afriani, Nita Agustian, Dede Rahman Aizah, Havina Nurul Almurdi Almurdi Alvarino Amel Yanis Amir, Arni Andani Eka Putra Anggiansyah Pohan Annisa Permata Sari, Annisa Permata Aprilia Susanti, Aprilia Ariadapa Rahadi, Didan Ariesko Gunanda Rangkuti Ariouso, Al H. Arni Amir Asmah Rahmat Asmah Rahmat Aulia Sepriadina Larasati Budianto, Raymond E. Cimi Ilmiawati, Cimi Daan Khambri Dede Agustian Rahman Desmawati Desmawati Dia Rofinda, Zelly Dia Dian Pertiwi Dina Arfiani Rusjdi Dwi Yulia Dwiharyani, Yuli Dwitya Elvira Elizabeth Bahar Endrinaldi Erlina Rustam Erly Erly Eti Yerizel Eva Decroli Fadrian, Fadrian Fatma Sri Wahyuni Fauzan, Rezky Fauziah Othman Fika Tri Anggraini Gestina Aliska Hanif, Pandu Muhammad Hanum, Fathiya Juwita Hasmiwati Hasnah Hasnah Hasnah, Hidayatul Havina Nurul Aizah Hirowati Ali Hirowati Ali, Hirowati Husnil Kadri Ilham Andhika Zulen Ilmiawati, Ilmiawati Jamsari Jamsari Kamila, Isna C. Keysha Putri Febiona Khairani, Salsabila P. Lily Syukriani Lim, Nurul Naimah binti Muhammad Khairuddin Linosefa Linosefa Lisana Shiddiqin Aliya Magza, Dekasih Tria Maharani, Malya CS. Malinda Meinapuri Masrul Djalal Meilisa Rahmawati Muhammad Ilham Mulfa Satria Asnel Nadya Mirenda Putri Nathaniel, Kevin Nelmi Silvia Noverika Windasari Noza Hilbertina Nugraha, Ikwandi C. Nurhayati, Nurhayati Nurmawaty - Olivia, Ivonne Pratama, Fajri Rahendra Pratama, Fajri Rahendra Puji Oktavia Puti Anggun Sari Rahayu, Yanti Rahmad Fajral Ilhami Rahmaddiansyah, Refa Rahmadian, Rizki Rahman, Awalil RK. Rahmatini . Rahmawati, Meilisa Rahmi, Wahida Rauza Sukma Rita Refa Rahmaddiansyah Refa Rahmaddiansyah Reza, Mohamad Risviani, Darayani Rizki Rahmadian Roza Silvia Rusti, Sukarsi Salsabila Putri Khairani Salsabila, Amilia Salsabila, Amilia Sari, Annisa Permata Shofiy, Alifya R. Sofia Mubarika Sofia Mubarika Haryana Suhaida. M. Jalil Sukarsi Rusti Susanti, Aprilia Syakira, Hasya Syakira, Hasya Theodora, Eillen Tofrizal Tofrizal Tofrizal Tyas, Syanindhita Wikanthining Ulya Uti Fasrini Utama, Bobby Indra Vella Amalia Wahida Rahmi Wahyudi, Dhyani P. Wirsma Arif Harahap Wiwi Pertiwi Yanwirasti - Yanwirasti - Yulia, Dwi Yuniar Lestari Yustini Alioes Zolkapli Eshak Zolkapli Eshak Zolkapli Eshak Zulfiqar, Yevri