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Journal : Jurnal Agripet

Efektivitas Pemberian Beberapa Preparat Hormon Prostaglandin Komersial terhadap Persentase Berahi Sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara Novika Ayuni Rambe; Tongku Nizwan Siregar; Teuku Armansyah TR; Gholib Gholib; Budianto Panjaitan; Mulyadi Adam; Dasrul Dasrul
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16317

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2 analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2-1 mempunyai efektivitas lebih baik dibandingkan PGF2-2 dan PGF2-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara.(Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera)ABSTRACT. The aim of this study was to determine the effect of differences PGF2 analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2-3 (cloprostenol 75 g/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.
Hubungan Kadar Progesteron pada Fase Awal Luteal dengan Kematian Embrio pada Sapi Aceh Budianto Panjaitan; Citra Chyntia Helwana; Nellita Meutia; Yusmadi Yusmadi; Tongku Nizwan Siregar; Dasrul Dasrul; Teuku Armansyah TR
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14881

Abstract

ABSTRAK. Progesteron merupakan hormon yang berperan penting dalam proses pemeliharaan kebuntingan dan dihasilkan oleh corpus luteum. Penelitian ini bertujuan mengetahui hubungan antara kadar hormon progesteron pada fase awal luteal dengan kematian embrio pada sapi Aceh. Dalam penelitian ini digunakan empat ekor sapi betina dewasa berumur 3-5 tahun, bobot badan 150-250 kg, sehat secara klinis, dan memiliki reproduksi normal. Sapi disinkronisasi menggunakan 5ml prostaglandin F2 alfa (PGF2) dengan pola penyuntikan ganda berinterval 11 hari. Koleksi sampel darah untuk pengukuran konsentrasi progesteron dilakukan pada hari ke-5, 6, dan 7 pasca inseminasi. Pengukuran konsentrasi progesteron dilakukan menggunakan metode enzymelinked-immunoassay (ELISA), pemeriksaan kebuntingan dan kematian embrio menggunakan metode transrektal ultrasonografi pada hari ke-25 pasca inseminasi. Pemeriksaan diulang setiap 10hari sampai hari ke-55 pasca inseminasi. Puncak sekresi progesteron pada sapi bunting dengan embrio yang bertahan hidup terdapat pada hari ke-7 (2,082 ng/ml), pada sapi Late Embryonic Mortality (LEM) di hari ke-5 (8,209 ng/ml) dan pada sapi tidak bunting di hari ke-7 (3,0511,157 ng/ml). Sekresi progesteron sapi LEM pada hari ke-5 sampai dengan ke-7 cenderung menurun sedangkan pada sapi yang bertahan hidup cenderung meningkat.(Correlation between progesterone levels in early luteal phase and embryonic death in Aceh cattle)ABSTRACT. Progesterone is an important hormone that functions to maintain pregnancy and is produced by the corpus luteum. The aim of this study was to see a correlation between progesterone and the incidence of embryonic death in Aceh cattle. This study used four adult female cows, 3-5 years old, 150-250 kg body weight, clinically healthy, and have a normal reproduction. The synchronized with 5 ml prostaglandin F2 alfa hormone, and double injection pattern with 11-day intervals. The blood was collected for progesterone measurements on 5th, 6th, 7th day post artificial insemination. Measurement of progesterone concentration was carried out using an enzymelinked-immunoassay (ELISA), while pregnancy and embryo mortality was performed using the trans-rectal ultrasonography method on the 25th day after insemination. The examination was repeated every 10 days until day 55th after insemination. Progesterone secretion peaks in pregnant cows were on day 7th (2.082 ng/ml), in cattle Late Embryonic Mortality (LEM) on day 5th (8.209 ng/ml) and in cattle not pregnant on day 7th (3.0511.157 ng/ml). The pattern of LEM progesterone secretion on days 5th to 7th tends to decrease while those that survive tend to increase.
Konsentrasi Hormon Testosteron Kerbau Simeulue dan Korelasinya dengan Tingkat Umur dan Lingkar Skrotum Qadarsina Qadarsina; Dasrul Dasrul; Sri Wahyuni
Jurnal Agripet Vol 19, No 1 (2019): Volume 19, No. 1, April 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i1.8692

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui konsentrasi hormon testosteron kerbau Simeulue dan korelasinya dengan tingkat umur dan lingkar skrotum. Sebanyak 15 ekor kerbau Simeulue jantan dibagi dalam tiga kelompok yaitu umur 2,1-3,0 tahun; 3,1-4,0 tahun, dan 4,1-5,0 tahun. Parameter yang diamati terdiri dari lingkar skrotum dan konsentrasi hormon testosteron. Data yang diperoleh dianalisis dengan analisis varian satu arah dan selanjutnya diuji dengan uji berganda Duncan. Untuk mengetahui hubungan antara umur, lingkar skrotum dengan konsentrasi hormon testosteron digunakan analisis regresi sederhana. Hasil analisis pada lingkar skrotum dan konsentrasi hormon testosteron memperlihatkan perbedaan yang nyata (P0,05). Lingkar skrotum dan kadar testosteron serum umur 2,1- 3,0 tahun berbeda secara nyata (P0,05) dengan umur 3,1- 4,0 tahun dan 4,1 - 5,0 tahun. Terdapat hubungan yang nyata (P0,05) antara konsentrasi hormon testosteron dengan umur dan lingkar skrotum, dengan persamaan regresi Y = -4,925-0,436 X1 + 0,697 X2. dengan nilai koefesien korelasi (r) sebesar 0,675 dan koefisien determinasi (r2) sebesar 0,455. Hasil ini menunjukkan bahwa lingkar skrotum (X2) berpengaruh sebesar 67,10 % terhadap konsentrasi hormon testosteron, lebih tinggi dibanding umur (X1) yang berpengaruh sebesar 46,60%. Kesimpulan, umur berpengaruh terhadap lingkar skrotum dan konsentrasi hormon testosteron kerbau Simeulue. Kerbau Simeulue berumur 4,1 - 5,0 tahun memiliki korelasi yang lebih kuat dari pada kerbau Simeulue umur 2,1 - 3,0 dan 3,1 - 4,0 tahun terhadap lingkar skrotum dan konsentrasi testosteron.(Testosterone hormone concentration of Simeulue buffalo and its correlation with age level and the scrotum circumference)ABSTRACT . The objective of this study was to determine the testosterone hormone concentration of Simeulue buffalo and its correlation with age level and the scrotum circumference. Fifteen male Simeulue buffalo were divided into three groups: 2.1 - 3.0 years; 3.1-4.0 and 4.1-5.0 years where each group consists of five buffaloes. The parameter observed consisted of age, scrotal circumference and testosterone hormone concentration. The data obtained were analyzed by one-way analysis of variance. The difference is tested with Duncan multiple tests. Meanwhile, simple regression analysis was used to testing the relationship between age and scrotal circumference with testosterone hormone concentration. The results showed that the scrotal circumferences and testosterone hormone concentration have significant differences (P 0.05). Scrotal circumference and testosterone level of 2.1-3.0 years were significantly different (P 0.05) with age 3.1- 4.0 years and 4.1 - 5.0 years. Additionally, there is a relationship (P0,05) between testosterone hormone concentration with age and scrotal circumference, with correlation coefficient value (r) = 0.675 and determination coefficient(r2) = 0,455, with regression equality Y =- 4,925 - 0,436 X1 + 0,697 X2. In conclusion, age affected the scrotal circumference and testosterone hormone concentration in the Simeulue buffalo. 4.1 - 5.0 years Simeulue buffalo has stronger correlation than 2.1 - 3.0 and 3.1 - 4.0 years of Simeulue Buffalo at scrotal circumference and testosterone concentration.
Analisis Motilitas Spermatozoa Sapi Aceh Setelah Pembekuan dalam Berbagai Konsentrasi Andromed Mukhlis Mukhlis; Dasrul Dasrul; Sugito Sugito
Jurnal Agripet Vol 17, No 2 (2017): Volume 17, No. 2, Oktober 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v17i2.8373

Abstract

ABSTRAK. Penelitian bertujuan mengetahui pengaruh konsentrasi pengencer AdroMed terhadap kualitas semen sapi aceh setelah proses pembekuan. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan pengenceran AndroMed. Kelompok A1: AndroMed 10% (5 ml AndroMed + 45 ml Aquadestilata), A2: AndroMed 15% (7,5 ml AndroMed + 42,5 ml Aquadestilata), A3: AndroMed 20%(10 ml AndroMed + 40 ml Aquadestilata) dan A4: AndroMed 25% (12,5 ml AndroMed + 37,5 ml Aquadestilata). Masing-masing kelompok diulangi sebanyak 6 kali. Variabel yang di amati pada penelitian ini adalah Motilitas spermatozoa yang diamati tiap kelompok setelah pembekuan yang selanjutnya dianalisis dengan analysis of variance (ANOVA) pola satu arah yang dilanjutkan dengan uji Duncant. Rata-rata persentase motilitas spermatozoa pada kelompok A1, A2, A3 dan A4 secara berturut-turut adalah 30,693,68%, 39,792,44%, 45,223,17% dan 42,424,24%. Hasil penelitian menunjukkan bahwa konsentrasi pengencer AndroMed berpengaruh secara nyata (P0,05) terhadap persentase motilitas spermatozoa sapi aceh. Persentase motilitas spermatozoa pada kelompok A1 tidak berbeda secara nyata dengan A2, dan keduanya berbeda secara nyata (P0,05) dibandingkan dengan kelompok A3dan A4. Konsentrasi AndroMed 20% lebih baik dari pada 10%, 15% dan 25% dalam mempertahankan motilitas spermatozoa sapi aceh setelah pembekuan.(Analysis of Aceh Cattle spermatozoa motility after freezing using Andromed with different concentration)ABSTRACT. The study aims to determine the effect of diluent concentration of AndroMed against the motility of aceh cattle spermatozoa following freezing process. This study uses a completely randomized design (CRD) with 4 treatment groups. Group 1 used diluent 15%; AndroMed, Grroup 2 diluent 15%; Group 3 with AndroMed 20%; and, Group 4 with AndroMed 25%. Each group was repeated 6 times. Motility of spermatozoa assessed which each group observed after freezing.The motility data obtained were analyzed by one way analysis of variance (ANOVA) followed by Duncant test. The average percentage of motility after freezing were found in the group A1, A2, A3 and A4 respectively are 30,69 3,68%, 39,79 2,44%, 45,22 3,17%, and 42,42 4,24%. Statistical analysis showed that the concentration of diluent AndroMed significantly affected (P0.05) the motility of aceh cattle spermatozoa. There is no significant difference of sperm motility percentage, between A2 with A1 treatment but both are significantly different (P0.05) compared to group A3. AndroMed concentration affects the motility of aceh cattle spermatozoa after freezing. The treatment of 20% AndroMed concentration were improved the quality of aceh cattle spermatozoa following freezing compared to those with AndroMed 10%, 15%, and 25%.
Pengaruh Pemberian Kombinasi Pakan Fermentasi dengan Multi Enzim dan Vitamin E dalam Ransum terhadap Peningkatan Kualitas Semen Ayam Arab Mustafa Mustafa; Dasrul Dasrul; M. Aman Yaman; Sri Wahyuni; Mustafa Sabri
Jurnal Agripet Vol 17, No 1 (2017): Volume 17, No. 1, April 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v17i1.6576

Abstract

ABSTRAK. Penelitian ini bertujuan mempelajari pengaruh pemberian kombinasi pakan fermentasi dengan multi enzim pencernaan dan vitamin E dalam ransum komersial terhadap peningkatan kualitas semen ayam arab. Penelitian ini menggunakan rancangan acak kelompok (RAK) yang terdiri dari 4 kelompok perlakuan dengan 5kali ulangan. 1) kontrol (P0) ayam diberikan ransum komersial 100 %, 2) perlakuan 1 (P1) ayam diberi 89,83% ransum komersial + 10% pakan fermentasi + 0,15% multi enzim + 0,02% vitaminE, (3) perlakuan 2 (P2) ayam diberi 79,83% ransum komersial + 20% pakan fermentasi + 0,15% multi enzim + 0,02% vitamin E, dan (4) perlakuan 3 (P3) ayam diberi 69,83% ransum komersial + 30% pakan fermentasi + 0,15% multi enzim + 0,02% vitamin E. Koleksi semen dilakukan pada hari ke 36 dengan metode masase. Data kualitas semen yang diperoleh dianalisis dengan menggunakan analysis of variance (ANOVA) satu arah yang dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan pemberian kombinasi pakan fermentasi dengan multi enzim dan vitamin E dalam ransum komersial berpengaruh secara nyata (P0,05) terhadap peningkatan volume, pH semen, konsentrasi spermatozoa, motilitas, spermatozoa hidup, dan penurunan abnormalitas ayam arab, namun tidak berbeda nyata (P0,05) terhadap warna, konsistensi dan gerakan massa spermatozoa. Dapat disimpulkan pemberian kombinasi pakan fermentasi dengan multi enzim dan vitamin E dalam ransum komersial dapat meningkatkan kualitas semen ayam arab.(Effect addition of fermented feed with the multi-enzyme digestion and vitamin E in commercial feed on improvement the quality of semen arab chicken)ABSTRACT. The study aimed to determine the effect of fermented feed combination with the multi enzymes digestion and vitamin E addition on the quality of semen arabs chicken. This research used a randomized block design (RAK) consisting four treatments with five replications : P0: 100% Commercial feed (524), P1: 89.83% commercial feed + 10% fermented feed + 0.02% viamint E + 0.15% multi enzyme, P2: 79.83% commercial feed + 20% fermented feed + 0.02% vitamin E + 0.15% multi enzyme, P3: 69.83% commercial feed + 30% fermented feed + 0.02% vitamin E + 0.05% multi enzyme. Semen sample was collected at 36th day using a massage method. Data obtained were analyzed using analysis of variance (ANOVA) and continued by Duncan test. The results showed that addition of combination of fermented feed with digestive multi enzymes and vitamin E has significantly effect (P0,05) on increases of the volume, pH, spermatozoa concentration, percentage of spermatozoa motility, spermatozoa life, and decreased the percentage of spermatozoa abnormalities. This research can be concluded that the addition of fermented feed combination with digestive multi enzymes and vitamin E able to improve the quality of arab chicken semen.
Rasio Jenis Kelamin Kelahiran Anak Kambing Peranakan Ettawa (PE) Hasil Inseminasi Buatan Menggunakan Spermatozoa Swim Up Sariadi Sariadi; Dasrul Dasrul; Muslim Akmal
Jurnal Agripet Vol 14, No 2 (2014): Volume 14, No. 2, Oktober 2014
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v14i2.1890

Abstract

(The ratio of birth sex of ettawa crossbreed goat by artificial insemination using spermatozoa swim up)ABSTRACT. The aim of this research was to determine the ratio of birth sex of ettawa crossbreed goat by artificial insemination using spermatozoa swim up. This research was at Reproduction Laboratory Faculty of Veterinary medicine Syiah Kuala university and Livestock Group SMD, Bener Meriah Regency. Fifteen female goats with age 8-24 months where used in this study and divided into 3 treatment groups (P0, P1, P2) and 5 replications of each. The first group (P0) inseminated with sperm without swim up; P1 and P2 inseminated with sperm which swim up for 5 minutes (P1) and 10 minutes (P2). The result of conception rates observation and sex ratio of children born tabulated into tables. The data was analyzed by using analysis of variance (ANOVA) one way pattern followed by the multiple test Duncan. The results showed that the conception rate and birth sex ratio of male born had significantly different (P0,05). The segregation of sperm which swim up increased the conception rate and birth sex ratio of male born.
Penambahan Tepung Daun Katuk (Saurupus Androgynus L. Merr) dalam Ransum Terhadap Pertambahan Berat Badan dan Lingkar Scrotum Kambing Jantan Peranakan Ettawa Dedhi Yustendi; Dasrul Dasrul; Didy Rachmadi
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i2.813

Abstract

The addition of katuk leaf meal (saurupus androgynus l.merr) in feeds on body weight gain and scrotal circumference male ettawa grade goatsABSTRACT. This research was aimed to study the effect of addition of katuk leaf meal (Sauropus androgynus L. Merr) in feed on body weight gain and scrotal circumference of male Ettawa grade goat. The research lasted for eight months, starting Agustus 2012 to Maret 2013, with feeding trial for two months. Twenty male Ettawa grade goats of 18 to 24 months old, were divided into 4 treatment groups. Treatment groups were added with 0% Katuk leaf meal (R0); 5% (R1); 10% (R2) and 15% (R3), with 5 goats in each replication. Treatment diets were feed in the morning and afternoon for 60 days. Weighing body weight and measuring scrotal circumference was done every week. Data were analyzed using analysis of variance (ANOVA) and if there was significantly different, the data were further tested using Duncan multiple range test. The result shows that the body weight gain of the treatment were, R0 (2,39 0,43 kg); R1 (2,84 0,36 kg); R2 (2,85 0,20 kg) and R3 (3,42 0,78 kg) respectively. Scrotal circumference were, R0 (0,74 0,10 cm); R1 (1,24 0,26 cm); R2 (1,26 0,40 cm) dan R3 (1,32 0,32 cm) respectively. The result of this research indicated that the addition of katuk leaf meal in feed had significant effects (P0,05) on body weight gain and scrotal circumference of Ettawa grade goat.
Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan Dasrul Dasrul; M. Aman Yaman; Zulfan Zulfan
Jurnal Agripet Vol 13, No 1 (2013): Volume 13, No. 1, April 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i1.546

Abstract

Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purposeABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the spermatozoa quality, sex ratio between X and Y, capacity of fertility indicated by conception rate and sex ratio of goat boar kids. Samples, used in this experiment, are fresh semen from Boer goat with high quality consists of 4 group treatments with 6 replications 1) group of spermatozoa without separation (control), 2) group of spermatozoa separated by percoll gradient density centrifugation 3 levels (P1), 5 levels (P2) and swine up (P3). The observed parameters are spermatozoa quality, X and Y spermatozoa ratio, fertilitys capacity and sex ratio on the birth. Quality examination of spermatozoa and identify X and Y spermatozoa is based on the standard method of WHO. The conception rate was based on the ratio of pregnant goat after the first insemination. Data of spermatozoa quality and spermatozoa ratio were analyzed by using analisis of variance (ANOVA) and further analysis by LSD if there were differences between treatments. The results of this experiment showed that spermatozoa quality Boer goat significantly reduced (p0,05) after separation with percoll gradient density centrifugation and swim up. Percentage spermatozoa X after percoll gradient density centrifugation was significantly higher (P0,05) compared to control and swim up. Meanwhile, the Y spermatozoa population was significantly higher (P0,05) after swim up treatment compared to percoll gradient density centrifugation and control. The percentage of sex ratio (male: female) after insemination from percoll gradient density centrifugation produced more female than male. On the other hand, insemination from swim up produced more male than female. Sex ratio produced from separation of percoll gradient density centrifugation, swim up was difference from control semen (P0,05). From this experiment, it was concluded that spermatozoa separation by percoll gradient density centrifugation and swim up can be used as one of the methods to separate X and Y spermatozoa and further can be applied to get preferred sex animals.
Efek Suplementasi ENERVON-C dan SANTA-e dalam Pakan terhadap Motilitas Spermatozoa Ayam Kampung Triva Murtina Lubis; Dasrul Dasrul; Hamdan Hamdan; Fauziah Fauziah
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i1.286

Abstract

Effect of Supplementation of ENERVON-C and SANTA-e in Feed on Sperm Motility of Local CocksABSTRACT. This study aimed to observe the effect of supplementation of ENERVON-C and SANTA-e in feed on sperm motility of local cocks. The research was conducted at Reproduction Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh. The research used 12 local cocks divided into 4 treatment groups : P0 (feed of 521), P1 (feed of 521 + 200 mg ENERVON-C/kg of feed), P2 (feed of 521 + 200 mg SANTA-e/kg feed) and P3 (feed of 521 + 100 mg ENERVON-C/kg of feed and 100 mg SANTA-e/kg of feed). During the research, all cocks were given 100 g of 521 feed/day. Feed with ENERVON-C and SANTA-e was given for 2 weeks before semen collections. Drink was given ad libitum. Data were analysed with analysis of variance and if there are effects among the treatments, analysis will be continued with Duncan Test. Results shows that supplementation of ENERVON-C or SANTA-e and their combination in feed can increase the percentage of sperm motility. There are significant differences (p0,05) between control and treatment groups. To sum up, supplementation of ENERVON-C or SANTA-e in feed can improve the quality of sperm (motility) of local cocks. The sperm quality is better after giving of ENERVON-C and SANTA-e together than single supplementation.
Efektivitas Penambahan Vitamin E (alfa-Tokoferol) dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman Dasrul Dasrul; Rasmaidar Rasmaidar; Abdul Harris
Jurnal Agripet Vol 12, No 2 (2012): Volume 12, No. 2, Oktober 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i2.196

Abstract

Effect of vitamin E addition (alfa-tokoferol) into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoaABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0), 0.1gr /100 ml medium (K1); 0.2gr/100 ml medium (K2) and 0.3 g / 100 ml medium (K4), each group was repeated 5 times. Examination of motility, viability and integrity of sperm membrane performed according to WHO standards. The data obtained were analyzed with one-way ANOVA and Duncan test. The average percentage of motility, viability and membrane integrity of spermatozoa in the addition of vitamin E were significantly different (P 0.05) compared to the control. Percentage of motility, viability and membrane integrity of spermatozoa in the group K2 significantly higher (P 0.05) compared with the group K3: K1 and K0. Percentage of motility, viability and sperm capacitation and sperm live on group K3 significantly higher (P 0.05) compared with the K1 and K0. While the percentage motility of spermatozoa in the group K1 higher were not significant (P 0.05) compared with the group K0. The addition of vitamin E in the medium on the process of washing spermatozoa Brahman cattle. The addition of vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in maintaining the percentage of motility and live spermatozoa Brahman cattle.
Co-Authors . Zulfan A. Rivai, Mohammad Abdul Harris Abdul Harris Abdullah Hamzah, Abdullah Abdullah, Mohd Agus Nashri Agung - Kusasti Agus Adria Alifa, Yola Amalia Sutriana Ardyes, Rizki Arman Sayuti Asnita Purnama Asri Gani Aulann'iam Aulann'iam Aulia, Usma Awaluddin Awaluddin Ayu, Onarisa Azhar Azhar Baehaqi Budianto Panjaitan Budianto Panjaitan Citra Chyntia Helwana Cut Dahlia Iskandar Cut Nila Thasmi Cut Nila Thasmi Cut Nila Thasmi Cut Nila Thasmi, Cut Nila Dedhi Yustendi Delli Lefiana, Delli Deslo, Jauhari Dian Masyitha Didy Rachmadi Dwinna Aliza Edy Fradinata Elsa Suarni Erdiansyah Rahmi Erwin Erwin Eskayanti Pasaribu Etriwati E Fatah, Khairul Fathurrahman, Fadhil Fauziah Fauziah Fitriani Fitriani Fitriani Fitriani Gholib Gholib Gholib Gholib, Gholib Ginta Riady Ginta Riady Hafizuddin Hafizuddin Hamdan Hamdan Hamdan Hamdan Hamdan Hamdan Hardjopranjot Hardjopranjot Hasan Al Aslam Hattanul Mulia HENNIVANDA HENNIVANDA Herrialfian, Herrialfian Husnurrizal, Husnurrizal Ibnu Khaldun Idawati Nasution Ismail Ismail Ismail Ismail Ismy, Jufriady Juli Melia Juli Melia Julia Kardin KARTINI ERIANI Khairul Fatah Kurniawan, Wilda Limbong, Ahmad Affandi Listin Handayani M Hasan M Yusuf M. Aman Yaman M. Aman Yaman Mahaputra L Mamfalutfi, Teuku Masda Admi Masruri Masruri Mauridatun Ramli Mefrianti Efendi Mirnasari, Mirnasari Mohd. Agus Nashri Abdullah Muhammad Hasan Muhammad Isa Muhammad Reza MUHAMMAD RIZAL Mukhlis Mukhlis Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Mustafa Mustafa Nazaruddin Nazaruddin Nellita Meutia Novi Afriani Nur Afriani Nur Novika Ayuni Rambe NURLIANA NURLIANA NURLIANA NURLIANA Pusaka, Semerdanta Qadarsina Qadarsina R. N. Selamet Raida Fuadi Rajuddin Rajuddin Rasmaidar Rasmaidar Rasmaidar Rasmaidar Razali Razali Rinidar Rinidar Riska, Raisa Mauliza Ronasky, T Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rosnizar, Rosnizar Rossy Septia Putri Rurini R Rurini R Rusli Rusli Rusnawati Rusnawati, Rusnawati Safrizal Rahman, Safrizal Sari, Nisa Sariadi Sariadi Siti Aisyah Siti Rizki Hardyana Siregar Sri Wahyuni Sri Wahyuni Sri Wahyuni Sudjarwo Sudjarwo Sugito - Sugito Sugito Sugito Sugito Syafruddin Syafruddin Syafruddin Syafruddin t fadrial karmil T. Armansyah Teuku Reza Ferasyi Teuku Zahrial Helmi Titit, Marcelus Ferdinandez Tongku Nizwan Siregar Tri Putri Purnama Sari Triva Murtina Lubis Triva Murtina Lubis Triva Murtina Lubis Veithzal Rivai Zainal Wahyuni, Sri Winaruddin Winaruddin Wira Asyudi Wulandari, Firstia Ritri Y, Bangun I Yusmadi Yusmadi Zaid Zaid Zarizki Taqwa Zulfan Zulfan Zulyazaini Zulyazaini Zulyazaini, Zulyazaini Zurnila MK