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All Journal ACTA VETERINARIA INDONESIANA Jurnal Pendidikan Vokasi Jurnal Veteriner Jurnal Ilmu Ternak International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Klinik Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Edik Informatika : Penelitian Bidang Komputer Sains dan Pendidikan Informatika Jurnal Bisnis, Manajemen, dan Informatika JETT (Jurnal Elektro dan Telekomunikasi Terapan) Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) JURNAL ILMIAH PETERNAKAN TERPADU Creative Research Journal ARSHI Veterinary Letters J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan ZIRAA'AH MAJALAH ILMIAH PERTANIAN At-Tajdid : Jurnal Pendidikan dan Pemikiran Islam Media Kontak Tani Ternak Jurnal Farmasi dan Sains Indonesia (JFSI) Journal of Science and Education (JSE) Jurnal PTI (Jurnal Pendidikan Teknologi Informasi) PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Bestari (JPMB) Arcitech: Journal of Computer Science and Artificial Intelligence Knowbase : International Journal of Knowledge in Database Journal of Applied Business and Technology Jurnal Kedokteran Hewan Farmers: Journal of Community Services The Indonesian Journal of Computer Science Jurnal Riset Sistem Informasi Jurnal Agripet ORAHUA : Jurnal Pengabdian Kepada Masyarakat Journal of Social and Community Development Journal of Medicine and Health
Rini Widyastuti
Laboratorium Reproduksi Dan Inseminasi Buatan, Departemen Produksi Ternak, Fakultas Peternakan, Universitas Padjadjaran
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Journal : Jurnal Agripet

 1 
Perbandingan Viabilitas Oosit Domba Pasca Vitrifikasi dengan Menggunakan Hemistraw dan Cryotop Kikin Winangun; Rini Widyastuti; Mas Rizky Anggun Adipurna Syamsunarno
Jurnal Agripet Vol 17, No 2 (2017): Volume 17, No. 2, Oktober 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v17i2.8131

Abstract

ABSTRAK.Tujuan dari penelitian ini adalah untuk mengkaji efek vitrifikasi dengan menggunakan dua buah system carrier yang berbeda terhadap viabilitas oosit domba yang telah dimaturasi secara in vitro. Oosit dibagi menjadi dua kelompok perlakuan, yaitu (i) divitrifikasi dengan menggunakan hemistraw (ii) divitrifikasi dengan menggunakan cryotop. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vitrifikasi serupa pada kedua jenis carrier yang digunakan untuk vitrifikasi. Oosit diletakkan dalam larutan equilibrasi yang mengandung konsentrasi permeable krioprotektan setengah dari larutan vitrifikasi. Setelah 15 menit, oosit ditransfer ke dalam media vitrifikasi yang mengandung 17% EG+17% DMSO +0, 65M sukrosa di dalam modified PBS yang disuplementasi dengan 20% fetal bovine serum. Total waktu yang digunakan untuk memaparkan oosit ke dalam larutan vitrifikasi adalah 30 detik. 5-8 oosit dipipet menggunakan kapiler gelas dan diletakkan/loading ke dalam carrier yang digunakan (hemistraw atau cryotop) kemudian langsung di paparkan ke dalam nitrogen cair. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vitrifikasi serupa pada kedua jenis carrier yang digunakan untuk vitrifikasi(Comparation of sheep oocyte viability after vitrification using hemistraw and cryotop)ABSTRACT.The aim of this study was to examine the effect vitrification using two different carrier system on the matured sheep oocytes viability. Oocytes were devided into two group (i) vitrified using hemistraw (ii) vitrified using cryotop. Oocytes placed into equilibration solution which is containing a half concentration permeable cryoprotectant of vitrification solution. After 15 minute, oocytes were transferred into vitrification solution containing 17% EG+17% DMSO +0, 65M sucrose in modified PBS supplemented with 20% fetal bovine serum. The total exposure time of oocytes to vitrification solution was 30 sec. Oocytes were pipetted into a glass capillary into group 5-8, and loaded into carrier (hemistraw or cryotop) then plugged into liquid nitrogen. After a week cryopreservation, oocytes were warmed and cultured in TCM 199 suplemented with 10% fetal bovine serum at 38.50 C under 5% CO2 for 3h. Oocytes viability was evaluated by re expansion, color and homogeneity of oocyte cytoplasm. Our results indicated that the oocytes viability after vitrification was similar from both of carrier for vitrification
Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium Rini Widyastuti; Siti Darodjah Rasad
Jurnal Agripet Vol 15, No 2 (2015): Volume 15, No. 2, Oktober 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v15i2.2417

Abstract

(Nuclear maturity of bovine oocyte after 24 hours ovary preservation)ABSTRACT. The objective of the research was to investigate their meiotic competence or nuclear maturity of bovine oocytes maturity in vitro after 24 hour preservation on 5C. Oocytes were collected by slicing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) homogenous ooplasm were cultured in maturity medium at 38C in humidified atmosphere of 5% CO2 incubator. After 24 hours, oocytes stained for nuclear maturitys evaluation. The proportion of oocytes at metaphase II (MII) was significantly difference (P 0.05) on oocytes that 24 hours preservated (44.21 3.04%) vs oocytes from fresh ovary (73.97 9.32%) (P0.05). These results indicated that 24 hours preservation bovines ovary on 50C cause decreases of nuclear oocyte maturity.
Pengaruh Keberadaan Corpus Luteum terhadap Kualitas dan Tingkat Maturasi Oosit Domba Lokal Umur Pubertas Awal Secara In Vitro Widyastuti, Rini; Syamsunarno, Mas Rizky Anggun Adipurna; Yusuf, Alvin; Ridhlo, Muhammad Rosyid; Prastowo, Sigit
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i2.12103

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 (4 lapis), grade 2 (34 lapis) dan grade 3 (02 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro.(Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty)ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by 4, 34, and 02 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production program
Co-Authors Abdullah, MT, Dr. Rijal Ade Fitri Rahmadani Ade Irma Suryani Ade Riki Kartana Aida Riyanti Alam, Sri Rahayu Alda Prayoga, Rama Alhuur, Ken Ratu Gaharizah Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alvin Yusuf An An Nurmeidiansyah, An An An An Yulianti Andi Hiroyuki Anggareni, Neni Annisa Yusrina Apriana, Diwi Arief Boediono Asarina, Shinta Ashabul Khairi Asmar Yulastri Asri Wulansari, Asri Bayani, Matni Syifa Budi Setiawan Dahlia Setiawan Dani Garnida Daud, Andre Rivianda Deru Indika Deru R Indika Desi Yunita Desti Ranihusna Diah Nugrahani Pristihadi Diding Latipudin Diky Ramdani dudi, dudi Dumono DWI ANDI NURMANTRIS Dwi Cipto Budinuryanto Dwi Wahyudha Wira Edianingsih, Primiani Elizamiharti Emini, Emini Fadhal M. Ahmad Fadjeri, Indrayati Fahmy Avicenna Faradana, Najma Rika Farel Fairuz Fatrosa, Triadmoko Denny Fauziah, Nisa Fauziah, Nisa Fauziati, Popi Firmansyah, Indra Fitri Rahmadani, Ade Ganefri Gea, Yufen Yuliandri Gita Widya Pradini Gusmanila Gusmanila Habibullah, Hafizh Henhen Heryaman Heryawan Kemal Mustafa Husna, Afifah Iin Susilawati Ilham Nugraha Iman Hernaman Inna Kholidasari Insy Nafisah Taufik Ismiraj, Muhammad Rifqi Jaqueline Sudiman Julius Pangayoman Karisma Mardatillah Karmila Suryani Karmila Suryani Kaseri, Kaseri Kasihani, Ni Nyoman Khairudin Khairudin Khoirinaya, Candrani Khotimah, Devi Syahrilia Kikin Winangun Kikin Winangun Krismadinata Krismadinata, Krismadinata Kurnia A Kamil Lubis, Alkaustariyah M. Dyon M. Ghozali M. Rosyid Ridlo MADE ASTAWAN Madeleine Priscilia Madeline Priscilla Mansyur . Mas Rizky A. A. Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A. Syamsunarno Mas Rizky Adipurna Anngun Syamsunarno Mohamad Ghozali Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muhammad Rifqi Ismiraj Muhammad Rosyid Ridhlo Muhammad Rosyid Ridlo Mulyanusa Ritongga Murlena, Murlena Mushawir, Andi Nahak, Martinus Nasution, Zuraidah Nelfira Nena Hilmia Neni Anggraeni Neni Anggraeni Nizwardi Jalinus Novi Mayasari Novi Mayasari Novrita, Sri Zulfia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurfitri Handayani, Nurfitri Nurul Ikhwan Nurul, Muhammad Pangestu, Muhammad Syahid Panji Petrus Kune Pisriwati, Siti Atwano Pradini, Gita W Priscilla, Madeleine Radial Anwar, Radial Rahmadani, Ade Fitri Rahmat Hidayat Ramdan Panigoro Rangga Setiawan Rangga Setiawan Refdinal, Refdinal Ridhlo, Muhammad Rosyid Ridwan Riska Amelia Rizka Amelia Rizki, Yovan Robianto, Sondi Romi Zamhir Ronnie Permana Sari, Aprilianna P.Z.N.L. SATRIYAS ILYAS Sefriani, Rini Sigit Prastowo Sigit PRASTOWO Sigit Prastowo Siswanto, Deny Hadi Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Soeparna Soeparna Soeparna Soeparna, Soeparna Sondi Robianto Sony Heru Sumarsono SONY HERU SUMARSONO Sony Heru Sumarsono SONY HERU SUMARSONO Sony Heru Sumarsono Sri Restu Ningsih Sri Suwarni Sudiman, Jaqueline Suhaida, Jihan Suharmanto, Dwi Sukamtono Sukamtono Syamsunarno, Mas Rizky A. A Syamsunarno, Mas Rizky A. A. Syamsunarno, Mas Rizky AA TAKDIR SAILI Toha . Toha, Toha Tya Gita Tyagita Hartady Tyagita Tyagita Uni Gamayani Wahyudin Wandi Syahindra Wibowo, Agus Wahyu Winangun, Kikin Winangun, Kikin Winsa, Larasati Yulcherlina Yusuf, Alvin Zein, Syaikha Dziyaulhaq