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All Journal ACTA VETERINARIA INDONESIANA Jurnal Pendidikan Vokasi Jurnal Veteriner Jurnal Ilmu Ternak International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Klinik Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Edik Informatika : Penelitian Bidang Komputer Sains dan Pendidikan Informatika Jurnal Bisnis, Manajemen, dan Informatika JETT (Jurnal Elektro dan Telekomunikasi Terapan) Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) JURNAL ILMIAH PETERNAKAN TERPADU Creative Research Journal ARSHI Veterinary Letters J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan ZIRAA'AH MAJALAH ILMIAH PERTANIAN At-Tajdid : Jurnal Pendidikan dan Pemikiran Islam Media Kontak Tani Ternak Jurnal Farmasi dan Sains Indonesia (JFSI) Journal of Science and Education (JSE) Jurnal PTI (Jurnal Pendidikan Teknologi Informasi) PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Bestari (JPMB) Arcitech: Journal of Computer Science and Artificial Intelligence Knowbase : International Journal of Knowledge in Database Journal of Applied Business and Technology Jurnal Kedokteran Hewan Farmers: Journal of Community Services The Indonesian Journal of Computer Science Jurnal Riset Sistem Informasi Jurnal Agripet ORAHUA : Jurnal Pengabdian Kepada Masyarakat Journal of Social and Community Development Journal of Medicine and Health

Rini Widyastuti

Laboratorium Reproduksi Dan Inseminasi Buatan, Departemen Produksi Ternak, Fakultas Peternakan, Universitas Padjadjaran

Author-ID : 2699294

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Anemia and Eosinophilia in Traditional Goat Farmers: Early Markers of Strongyle Zoonoses (ANEMIA DAN EOSINOFILIA PADA PETERNAK KAMBING TRADISIONAL: PENANDA AWAL ZOONOSIS STRONGYLUS) Insy Nafisah Taufik; Henhen Heryaman; Rini Widyastuti; Mas Rizky A. A. Syamsunarno; Mohammad Ghozali
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.548 KB)

The spread of zoonoses between humans and animals can increase with the presence of specific contacts between the two. As well as intensive contact between farmer and goat. Anemia is a common hematologic change encountered in infection and zoonoses so it can be used as an early indicator for zoonoses. Nevertheless, anemia itself cannot lead to a specific etiology thus differential leukocyte count and goat fecal examination can be a good supporting test. Understanding the etiologies of the disease is critically important in preventing the decline in human’s quality of life as well as economic losses. This cross-sectional study included 30 farmers who were recruited by purposive sampling method. They agreed to do a CBC examination with 22 parameters. From the test, 37% of them showed mild anemia. There were 45% farmers with microcytic hypochromic anemia while the rest had normocytic normochromic anemia. There was an increased number in eosinophil and segmented neutrophil from anemic farmers, 18% and 9% respectively. Besides, 13% of goats physical examination indicated as below normal conditions characterized by lean bodies, lymph node swelling, and pale mucosa. Whereas 38% goat’s fecal test showed evidence of Strongyle eggs. The research suggests a possible interrelation between animals, humans, and the environment with an increased risk of zoonoses. Anemia and eosinophilia that occur can be an early marker which will be decisive not only for the success of the disease progression reduction but also the long term suppression of the economic loss.

Low Concentration of Ethylene Glycol Improved Recovery Rate of Human Spermatozoa After Vitrification (ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKAN RECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) Rini Widyastuti; Sony Heru Sumarsono; Arief Boediono; Siti Darodjah Rasad
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.367 KB)

The use of cryoprotectants for the cryopreservation of human spermatozoa, oocytes, zygote, earlycleavage stage of embryos and blastocyst is an integral part of almost every human In Vitro Fertilizationprogram. Moreover, the cryopreservation of these types of cells by direct plunging into liquid nitrogen (-196°C) usually requires a high concentration of cryoprotectant with a consequent of cytotoxic effect. Theaim of this study was to observe the effect of ethylene glycol concentration on the spermatozoa recoveryrate following vitrification process. Earle’s balanced salt solution + 0.25 M sukrosa + 1 % human albumineserum as basic solution supplemented with some different concentrations of etylene glycol (ie: 36.25%;18.25%; 9.12%; 4.56%; 1.14% and 0.57%) were used to evaluate the motility and viability of spermatozoafollowing vitrification. Human’s spermatozoa from ejaculates with progressive motility and viability above50% were used as samples. Samples were mixed with vitrification solution and then loaded into 0.25 mLstraws, equilibrated for 10 minutes at room temperature before plunged into liquid nitrogen. Spermatozoathawing was done at 24 hours after the vitrification. The results showed that, the decrease of spermatozoamotility and viability were observed at the highest (100%, 96.70%, respectively) in the samples that wereadded with vitrification medium contained 36.25% of ethylene glycol. On the other hand, the decrease ofthe spermatozoa motility and viability were found at the lowest (14.11%, 43.81 %, respectively) in thesamples without ethylene glycol supplementation. It can be concluded that the highest spermatozoa recoveryrate was obtained from the vitrification using a low concentration of ethylene glycol.

Mice Oocytes Respond after Vitrification Followed by Artifical Activation Using a Various Concentration of Strontium Chloride and Cytochalasin B Muhammad Rosyid Ridlo; Rini Widyastuti; Alkaustariyah Lubis; Mokhamad Fahrudin; Arief Boediono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.945 KB)

Oocyte vitrification is the important part of gamete preservation for further purpose. The objective of this study was to evaluate the response or development of vitrified-mice oocyte following activation using various concentrations of Strontium Chloride (Sr Cl2). Oocytes were collected from superovulation-induced female mice. Oocytes vitrification was then performed using a gradual equilibration of 2 M Ethylene Glycol in 0.25 M sucrose and 7 M Ethylene Glycol on 0.5 sucrose. Subsequently, the vitrified oocytes were thawed and activated using various Strontium Chloride concentration in each group. Control 1 is unvitrified oocyte and without Sr Cl2. Control 2 is unvitrified oocyte then activated by 20 mM Sr Cl2. Zero (0) mM Sr Cl2 is vitrified oocyte without Sr Cl2. Group Ten (10) mM is vitrified oocyte then activated by 10 mM Sr Cl2. Group Twenty (20) mM is vitrified oocyte then activated by 20 mM Sr Cl2. The viability of vitrified-thawed oocytes was observed based on ooplasm integrity. Whereas the oocytes respond to artificial activation was observed based on pronucleus formation after 10 hours of activation. The result showed that 39% of oocyte degenerated following vitrification. The respond of vitrified-thawed oocytes following artificial activation using Strontium Chloride was significantly lower compared to fresh oocytes (p<0,05). Interestingly the highest percentage of activated oocytes (36.36%) was present in a group achieved 20 mM Strontium Chloride. As conclusion is Strontium Chloride 20mM has a best result (36,36%) to activate vitrified oocyte than 0 mM and 10 mM of Strontium Chloride.

Derajat Pemulihan dan Persentase Spermatozoa X dan Y Kambing Peranakan Etawah Setelah Separasi dengan Gradient Percoll (RECOVERY RATE AND PERCENTAGE OF SPERMATOZOA X AND Y OF ETAWAH CROSSBREED GOAT AFTER SEXING WITH GRADIENTT PERCOLL) Siti Darodjah Rasad; Rangga Setiawan; Nurcholidah Solihati; Rini Widyastuti; Ilham Nugraha
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Aim of the research is to find out recovery rate and percentage spermatozoa X and Y after sexing with gradientt Percoll in Etawah Crossbreed Goat. Extender was used TRIS Egg Yolk. The semen sample from five male Etawah Crossbreed Goat were used in this research. Gradientt percoll performed 10 fraction layer with centrifugation time 10 minute at 2500 rpm. The data were analyzed descriptively. Result of the research shows that average recovery rate of spermatozoa X is 50.96±26.07 % and spermatozoa Y is 44.13±6.11 %. Average percentage of X sperm is 78.00±7.06 % and Y sperm is 76.10±5.95 % .

In-vivo Mice Pre-Implantation Embryo Development after Oral Administration Ethanolic Extract of Cogon Grass Roots (Imperata cylindrica L) Jaqueline Sudiman; Rini Widyastuti; Madeline Priscilla; Alkaustariyah Lubis; Mas Rizky Anggun Adipurna Syamsunarno; Sony Heru Sumarsono
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Cogon grass (Imperata cylindrica L) is known as a medicinal plant that is scattered almost worldwide. Despite its role that inhibits another plant’s growth, cogon grass possesses several benefits in health. This research has to identify the effect of short-term gavage ethanolic extract of cogon grass roots (CGG) to in-vivo mice preimplantation embryo development. A total of 60 female mice were divided into control and treatment groups, dosages at 90 and 115 mg/kg of body weight of CGG, orally gavage for 20 days. The superovulation of mice was done at the end of the CGG treatment by injecting 5 IU Pregnant Mare Serum Gonadotropin (PMSG) and after 48 hours, followed by 5 IU Human Chorionic Gonadotropin (hCG) injection and directly the mice were mated. The mating rate was checked by the appearance of the vaginal plug 12 hours after hCG injection. Mice were sacrificed, the oviducts and cornua of uteri were isolated to collect the oocytes and embryonic cells by flushing the oviducts and cornua uteri with Phosphate-buffered saline (PBS). The effects of CGG as an antifertility were evaluated by measuring the number of oocytes, fertilization, and in-vitro embryo development rates. The results showed significantly reduced about half of the mating rate in the 115 mg/kg BW group (p<0.05) compared to control. However, the 90 mg/kg BW dose reduced 20% mating rate compared to control, and not significant (p>0.05). In all treatment groups, only half oocytes fertilized. The cleavage and blastocyst rate in 115 mg/kg BW group were significantly lower compared to the control group (p<0.05). In conclusion, oral gavage of cogon grass root ethanolic extract disrupts the mating process and development of in-vivo mice preimplantation embryo development.

Oral Administration of Cogongrass (Imperata cylindrica L) Root Ethanol- Extract causes Mouse Epididymal Sperm Abnormality (PEMBERIAN EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICA L) SECARA ORAL MENYEBABKAN ABNORMALITAS SPERMA EPIDIDYMIS MENCIT) Rini Widyastuti; Jaqueline Sudiman; Tyagita Tyagita; Mas Rizky Adipurna Anggun Syamsunarno; Sony Heru Sumarsono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.412 KB)

Sperm morphology is an important parameter to be observed in the male fertility. Some of the bioactive compounds of cogongrass root such as alkaloid and terpenoid, affect male fertility by interference the spermatogenesis. The objective of the study was to observe the effect of cogongrass root ethanol extract on mouse sperm morphology. This study was carried out by oral administration of two different doses i.e 90 and 115 mg/kg body weight of cogongrass root ethanol extract into 8-10 weeks old DDY strain mice for 14 days to evaluated the acute effect due to the administration of cogongrass root ethanol extract on mouse sperm morphology. The results showed that treatment with cogongrass root ethanol extract significantly increased sperm abnormalities followed a dose depending pattern (p<0.05). Interestingly, the administration of cogongrass root extract did not affect sperm head morphology but tailless, folded and bent sperm increased linearly with the administration dose of cogongrass root ethanol extract. In conclusion, cogongrass root ethanol extract causes secondary sperm abnormalitties on mouse sperm.

The Effect of Ethanol Extract of Cogon Grass Root (Imperata cylindrica) to Estrus Cycle and Metabolite Profile in Female Mice Sondi Robianto; Mas Rizky Adipurna Anngun Syamsunarno; Alkaustariyah Lubis; Madeleine Priscilia; Neni Anggraeni; M. Ghozali; Mulyanusa Ritongga; Rini Widyastuti
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The use of herbs as contraception has become the focus of current contraceptive development.. In the previous study, the extract ethanol of cogon grass roots (Imperata cylindrica) has the effect of decreasing sperm production and changing metabolite profiles in male mice. However, the effect of this Indonesian well-known herb is still indefinite in female.The purpose of this study was to investigate the effect of ethanol extract of cogon grass roots to the fertility of female mice. The study was conducted at Animal Laboratory of Faculty of Medicine and Central laboratory Universitas Padjadjaran from November to December 2017. Mice were given ethanol extract of cogon grass roots 90 and 115 mg/kg body weight per oral. After three weeks, vaginal cytology was observed and metabolite profiles were measured. The results showed a shortening of the estrus phase in 90 and 115 mg/kg treatment group. However, the level of glucose and cholesterol in serum were not different among groups. This study indicated the extract ethanol of cogon grass roots has potential effect as contraceptive agent and the effect is independent from metabolite profiles. Keywords: Cogon Grass, Imperata cylindrica, Estrus Cycle Phase, Metabolite Profile

Co-Authors Abdullah, MT, Dr. Rijal Ade Fitri Rahmadani Ade Irma Suryani Ade Riki Kartana Aida Riyanti Alam, Sri Rahayu Alda Prayoga, Rama Alhuur, Ken Ratu Gaharizah Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alvin Yusuf An An Nurmeidiansyah, An An An An Yulianti Andi Hiroyuki Anggareni, Neni Annisa Yusrina Apriana, Diwi Arief Boediono Asarina, Shinta Ashabul Khairi Asmar Yulastri Asri Wulansari, Asri Bayani, Matni Syifa Budi Setiawan Dahlia Setiawan Dani Garnida Daud, Andre Rivianda Deru Indika Deru R Indika Desi Yunita Desti Ranihusna Diah Nugrahani Pristihadi Diding Latipudin Diky Ramdani dudi, dudi Dumono DWI ANDI NURMANTRIS Dwi Cipto Budinuryanto Dwi Wahyudha Wira Edianingsih, Primiani Elizamiharti Emini, Emini Fadhal M. Ahmad Fadjeri, Indrayati Fahmy Avicenna Faradana, Najma Rika Farel Fairuz Fatrosa, Triadmoko Denny Fauziah, Nisa Fauziah, Nisa Fauziati, Popi Firmansyah, Indra Fitri Rahmadani, Ade Ganefri Gea, Yufen Yuliandri Gita Widya Pradini Gusmanila Gusmanila Habibullah, Hafizh Henhen Heryaman Heryawan Kemal Mustafa Husna, Afifah Iin Susilawati Ilham Nugraha Iman Hernaman Inna Kholidasari Insy Nafisah Taufik Ismiraj, Muhammad Rifqi Jaqueline Sudiman Julius Pangayoman Karisma Mardatillah Karmila Suryani Karmila Suryani Kaseri, Kaseri Kasihani, Ni Nyoman Khairudin Khairudin Khoirinaya, Candrani Khotimah, Devi Syahrilia Kikin Winangun Kikin Winangun Krismadinata Krismadinata, Krismadinata Kurnia A Kamil Lubis, Alkaustariyah M. Dyon M. Ghozali M. Rosyid Ridlo MADE ASTAWAN Madeleine Priscilia Madeline Priscilla Mansyur . Mas Rizky A. A. Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A. Syamsunarno Mas Rizky Adipurna Anngun Syamsunarno Mohamad Ghozali Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muhammad Rifqi Ismiraj Muhammad Rosyid Ridhlo Muhammad Rosyid Ridlo Mulyanusa Ritongga Murlena, Murlena Mushawir, Andi Nahak, Martinus Nasution, Zuraidah Nelfira Nena Hilmia Neni Anggraeni Neni Anggraeni Nizwardi Jalinus Novi Mayasari Novi Mayasari Novrita, Sri Zulfia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurfitri Handayani, Nurfitri Nurul Ikhwan Nurul, Muhammad Pangestu, Muhammad Syahid Panji Petrus Kune Pisriwati, Siti Atwano Pradini, Gita W Priscilla, Madeleine Radial Anwar, Radial Rahmadani, Ade Fitri Rahmat Hidayat Ramdan Panigoro Rangga Setiawan Rangga Setiawan Refdinal, Refdinal Ridhlo, Muhammad Rosyid Ridwan Riska Amelia Rizka Amelia Rizki, Yovan Robianto, Sondi Romi Zamhir Ronnie Permana Sari, Aprilianna P.Z.N.L. SATRIYAS ILYAS Sefriani, Rini Sigit Prastowo Sigit PRASTOWO Sigit Prastowo Siswanto, Deny Hadi Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Soeparna Soeparna Soeparna Soeparna, Soeparna Sondi Robianto Sony Heru Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO Sony Heru Sumarsono SONY HERU SUMARSONO Sri Restu Ningsih Sri Suwarni Sudiman, Jaqueline Suhaida, Jihan Suharmanto, Dwi Sukamtono Sukamtono Syamsunarno, Mas Rizky A. A Syamsunarno, Mas Rizky A. A. Syamsunarno, Mas Rizky AA TAKDIR SAILI Toha . Toha, Toha Tya Gita Tyagita Hartady Tyagita Tyagita Uni Gamayani Wahyudin Wandi Syahindra Wibowo, Agus Wahyu Winangun, Kikin Winangun, Kikin Winsa, Larasati Yulcherlina Yusuf, Alvin Zein, Syaikha Dziyaulhaq

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