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All Journal Jurnal Biologi Udayana Bioscience Bioeducation Journal Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Biotek Medisiana Indonesia Althea Medical Journal Biota Biomedika BIO-SITE |BIOLOGI Sains Terapan Jurnal Penelitian dan Pembelajaran IPA Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Jurnal Agrotek Tropika Bio-Lectura Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Majalah Kesehatan Pharmamedika Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi IJECA (International Journal of Education and Curriculum Application) Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Biosense Halal Research Journal TROPICAL GENETICS Scientific Journal MASALIQ: Jurnal Pendidikan dan Sains Tropical Genetics Jurnal Serambi Biologi Berita Biologi
Dwi Hilda Putri
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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UJI AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT PSEUDOMONAS BERFLUORESEN TERHADAP Escherichia coli, Staphylococcus aureus DAN Candida Eka Nuraini, Fauzi; Hilda Putri, Dwi; Advinda, Linda; Irdawati; Handayani, Dezi; Mardhiyah Nazri, Laila; Wulandari, Tesya; Febrianti, Liza
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.7989

Abstract

Antimicrobial resistance is increasing every year. New and better antimicrobial sources are needed. One source of antimicrobials that can be explored is from endophytic bacteria. This study aims to test the ability of fluorescent Pseudomonas endophytic bacteria from the roots of plantain lemongrass in producing antimicrobial compounds. The type of research is descriptive. The antimicrobial activity of fluorescent Pseudomonas isolates was tested using the point inoculation method using test microbes E. coli, S. aureus and C. albicans. The clear zone was observed after 24 hours. The results showed that fluorescent Pseudomonas isolates were able to inhibit E. coli with a diameter of 3.9 mm found in isolate 51. S. aureus bacteria had an inhibition zone of 9.4 mm in isolate 89. Interesting results were shown by isolate 51 in inhibiting C. albicans with an inhibition zone diameter of 29.9 mm. These differences in response are thought to be related to variations in cell wall structure and the presence of an outer membrane in Gram-negative bacteria, which inhibits the penetration of active compounds. Overall, fluorescent Pseudomonas endophytic bacteria from the roots of the plantain have the potential to be developed as a source of new antifungal candidates against C. albicans, while their antibacterial activity still needs to be optimized through compound purification and further testing.
AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT (Pseudomonas Berfluoresen) DARI AKAR PISANG BUAI (Musa x paradisiaca L.) Astrid; Hilda Putri, Dwi; Handayani, Dezi
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8176

Abstract

Infectious diseases caused by pathogenic bacteria and fungi remain a global health problem, particularly due to the increasing incidence of antimicrobial resistance, highlighting the need for effective alternative antimicrobial sources. Endophytic bacteria, especially fluorescent Pseudomonas, are known to produce secondary metabolites with antibacterial and antifungal properties. This study aimed to evaluate the antimicrobial activity of endophytic fluorescent Pseudomonas isolated from the roots of banana buai (Musa × paradisiaca L.) and to identify isolates with the highest activity. This experimental study employed a Completely Randomized Design (CRD) using 44 isolates with three replications. Antimicrobial activity was tested in vitro using the spot inoculation method against Escherichia coli, Staphylococcus aureus, and Candida albicans. The observed parameter was the diameter of the inhibition zone, which was analyzed using analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test. The results showed that antimicrobial activity varied among isolates and was specific to the test microorganisms. Isolate PFPB 32 exhibited the highest activity against E. coli and S. aureus, while isolate PFPB 300 showed the strongest antifungal activity against C. albicans. These findings indicate that endophytic fluorescent Pseudomonas from banana buai roots have potential as a source of antimicrobial agents, particularly antifungal agents against Candida albicans
Optimization of Manganese Peroxidase Enzyme Production from Pleurotus ostreatus Mushroom in Organik Medium Afroza, Faiza; Putri, Dwi Hilda; Irdawati, Irdawati; Farma, Siska Alicia; Andriani, Ade; Saskiawan, Iwan
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.8892

Abstract

The production of manganase peroxidase (MnP, a key lignolityc enzyme with broad applications in bioremediation and industrial processing, can be significantly enhanced trough the optimation of medium composition and fermentation time. This study aimed to determine the optimal organic medium, concentration, and incubation period for MnP production by Pleurotus ostreatus. Various plant-base substrates such as brown rice, mungbean, almond, red bean soybean, and potato dextrose broth (PDB) were evaluated using a descriptive method, withvthe obtained data calculated and analyzed in Excel, and the results presented in the form of diagrams. The results showed that brown rice medium is the most optimal medium to support the produvction of MnP (981,6 U/L), attribute to its high carbohydrate and micronutrien content. Futher optimization revealed that a consentration of 7,5%, while fermentation time optimization indicated that the 9th day was optima, producing up to 3207,1 U/L of enzyme activity. These findings suggest that selecting the right substrate and fermentation conditions can substantially improve MnP synthesis by P. ostreatus, making it a promising strategy for sustainable enzyme production and future biotecnological application. For future studies, it is recommended to explore large scale fermentation systems and alternative agro industrial waste susbtrates to further enhance enzyme yield and cost efficiency
LITERATUR REVIEW: POTENSI LIMBAH PERTANIAN DAN PERKEBUNAN DALAM PRODUKSI BIOETANOL GENERASI KEDUA DI INDONESIA: Indonesia Winardi, Fhuji; Putri, Dwi Hilda
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6253

Abstract

Permintaan energi global yang meningkat dan penipisan sumber daya fosil mendorong pencarian bahan bakar terbarukan, seperti bioetanol generasi kedua dari limbah lignoselulosa. Namun, produksinya menghadapi tantangan teknis, terutama pada pretreatment dan efisiensi konversi akibat kompleksitas struktur biomassa. Artikel review ini bertujuan menganalisis metode pemanfaatan limbah pertanian dan perkebunan Indonesia untuk produksi bioetanol generasi kedua, mengidentifikasi peluang dan tantangan, serta memberikan rekomendasi strategis. Metode yang digunakan adalah literature review terhadap artikel terpublikasi dalam lima tahun terakhir dari Google Scholar dan ScienceDirect dengan kata kunci terkait. Hasil review menunjukkan keragaman bahan baku seperti TKKS, ampas tebu, kulit buah dan dominasi pretreatment kimia serta hidrolisis asam untuk mendegradasi lignin. Fermentasi dengan Saccharomyces cerevisiae dengan konsentrasi dan waktu bervariasi menghasilkan variasi yield bioetanol, yield bioetanol tertinggi dari limbah kulit buah nanas dengan pretreatment intensif sebesar 59%. Disimpulkan bahwa keberhasilan produksi bergantung pada sinergi optimal pretreatment, hidrolisis, dan fermentasi, dengan kebutuhan inovasi pada metode ramah lingkungan dan optimasi proses.
PENGARUH RADIASI SMARTPHONE DALAM KONDISI AIRPLANE MODE TERHADAP HISTOLOGI GINJAL MENCIT BETINA Ayuningtyas, Maulia Indah; Mellani Rachma; Fhuji Winardi; Afandi, Echa Azkia; Yusni Atifah; Dwi Hilda Putri
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6697

Abstract

The rapid advancement of technology and information has led to a significant increase in smartphone usage in Indonesia, reaching 67.88% in 2022. Smartphones emit non-ionizing electromagnetic waves that may pose biological effects, particularly on the kidneys, which play a crucial role in maintaining the body's homeostasis. Previous studies have reported that electromagnetic radiation can induce histological damage in the kidneys through oxidative stress mechanisms, including tubular narrowing, Bowman’s capsule dilation, and tubular epithelial necrosis. Airplane mode is known to significantly reduce electromagnetic radiation exposure. This study aimed to determine the effect of smartphone radiation in airplane mode on the kidney histology of female mice. An experimental design was employed using four female mice divided into one control group and three treatment groups, which were exposed to a smartphone in airplane mode for 4, 6, and 8 hours per day for 7 consecutive days. Kidney tissues were processed using paraffin embedding and stained with Hematoxylin–Eosin for microscopic examination. The results showed no significant histological differences between the control and treatment groups. The glomerulus, Bowman’s capsule space, and proximal and distal convoluted tubules appeared normal without signs of narrowing, dilation, hemorrhage, or necrosis. These findings indicate that smartphone radiation in airplane mode does not cause histological damage to the kidneys of female mice.
UJI ANTAGONIS BAKTERI ENDOFIT Pseudomonas BERFLUORESEN TERHADAP RALSTONIA SOLANACEARUM PENYEBAB LAYU TANAMAN TOMAT DAN NILAM Nazri, Laila Mardhiyah; Putri, Dwi Hilda; Advinda, Linda; Nuraini, Fauzi Eka; Handayani, Dezi; Chatri, Moralita
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6876

Abstract

Penyakit layu bakteri yang disebabkan oleh Ralstonia solanacearum merupakan salah satu kendala utama dalam budidaya tomat dan nilam karena mampu menurunkan produktivitas secara signifikan. Penggunaan agen hayati seperti pseudomonas berfluoresen menjadi alternatif pengendalian yang lebih aman dan ramah lingkungan. Penelitian ini dilakukan untuk mengetahui kemampuan antagonis tujuh isolat bakteri endofit pseudomonas berfluoresen yang berasal dari akar pisang buai dalam menghambat pertumbuhan R. solanacearum yang menginfeksi tomat dan nilam. Penelitian memakai pendekatan deskriptif-kuantitatif dan dilaksanakan di Laboratorium Biologi FMIPA UNP pada Agustus–November 2025. Isolat pseudomonas berfluoresen dan R. solanacearum diremajakan pada media selektif, kemudian diuji antagonismenya menggunakan metode swab pada medium MHA. Suspensi patogen distandarkan pada McFarland 0,5, sedangkan isolat antagonis diinokulasikan secara titik. Zona hambat yang terbentuk setelah inkubasi 24 jam diukur menggunakan jangka sorong dan dianalisis secara deskriptif. Hasil penelitian menunjukkan adanya variasi kemampuan penghambatan antar isolat. Pada patogen tomat, isolat PfB305, PfB309, PfB311, dan PfB322 menghasilkan zona hambat yang relatif besar, sedangkan PfB32, PfB51, dan PfB52 tidak menunjukkan aktivitas antagonis. Pada patogen nilam, tiga isolat yang sebelumnya tidak aktif pada patogen tomat justru menghasilkan zona hambat, sementara empat isolat lainnya tidak membentuk zona hambat. Penelitian ini menunjukkan bahwa efektivitas pseudomonas berfluoresen dipengaruhi oleh spesifisitas isolat terhadap patogen serta potensi produksi metabolit antibakteri
Etnobotani Tumbuhan Obat di Nagari Mungka Kecamatan Mungka Kabupaten Lima Puluh Kota Farras, Fadhila Annisa; Putri, Dwi Hilda; Chatri , Moralita; Handayani , Dezi
Scientific Journal Vol. 5 No. 2 (2026): SCIENA Volume V No 2, March 2026
Publisher : CV. AKBAR PUTRA MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56260/sciena.v5i2.318

Abstract

Pendahuluan: Tumbuhan Obat merupakan tumbuhan yang digunakan sebagai bahan baku pengobatan dalam mengatasi penyakit. Tujuan penelitian: Mengetahui jenis-jenis tumbuhan obat, organ tumbuhan yang digunakan serta khasiat dan cara penggunaannya di Nagari Mungka. Metode : Jenis penelitian ini adalah deskriptif eksploratif. Sampel penelitian ini berjumlah 23 responden. Analisis data secara kualitatif dengan dukungan pustaka ilmiah, kemudian data yang diperoleh disajikan dalam bentuk tabel. Hasil : ditemukan 39 jenis tumbuhan obat yang terdiri dari 29 familia. Tumbuhan obat yang banyak ditemukan yaitu familia Euphorbiaceae, Fabaceae, Lamiaceae masing-masing sebanyak 3 species, Organ tumbuhan yang banyak digunakan adalah daun. Cara pengolahan tumbuhan obat yang digunakan dengan cara direbus, dibakar, digiling atau dihaluskan, dihancurkan, dan diremas. Sedangkan untuk pemanfaatan tumbuhan obat ada yang diminum, diurut, diusap, dioles, dtempel, digosok dan dikonsumsi langsung. Kesimpulan : Masyarakat di Nagari Mungka masih memanfaatkan tumbuhan sebagai obat tradisional.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abdul Razak Achyar, Afifatul ADE ANDRIANI AERMA HASTUTY Afandi, Echa Azkia Afionita, Santi Afroza, Faiza Ahmad Wibisana, Ahmad Albar, Rahmat Alfatimah Azzahra, Balkis Amanda, Nifsa Riski Andri Damayanti, Ndaru Annisa Irna Putri Annisa Khaira Arlina, Sistika Armen Armen Astrid Atifah, Yusni Aulia Yunita Aulia, Ony Nattasha Aura Iga Maharani Ayuningtyas, Maulia Indah Az-Zahra, Fauziah Azwir Anhar Berlinda Paradisa, Yashanti Cania Dewi, Rahmawitra Chatri, Moralita Chatri Cindy Pramila Darussyamsu, Rahmawati Des M Dezi Handayani Dezi Handayani Dina Sukma dina vaniana Dinda Sahara Djoelvinanda, Habibullah Edwin Edwin Efliani Efliani Eka Nuraini, Fauzi Eka Vidya Putra Elsa Alfiyanti Elsa Badriyya Elsa Yuniarti Fahra Fahra Fahra, Fahra Farras, Fadhila Annisa Fatma Rahmadhani Febri Doni Febrianti, Liza Febrina Anggiastanti Feby Yeriska Fevria, Resti Fhuji Winardi Fira Safitri Fransisco, Sandi Fronica, Imelda Ghiffari, Muhammad Gustina Indriati Handayani , Dezi Hartati Hartati Heffi Alberida Helsa Rahmatika Hengki Saputra Herman, Reni Herman, Reni Husnul Khatimah Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Irvan Faizal IWAN SASKIAWAN Jamhari Jamhari Jannah Koftiah Jihan Rezi Okanti Kardiman, Reki Karlini Oktarina Kiki Amelia, Kiki Larasati Arum Utami Linda Advinda Lufri Lufri Mades Fifendy Mahjani Mahjani Maiyusri Eka Putri Mantoviana, Tiffany Mardhiyah Nazri, Laila Marissa Angelina Mellani Rachma Miftahul Jannah Miftahul Rahmi Moca Faulina putri Monhartini, Monhartini Moralita Chatri Muhyiatul Fadilah Muhyiatul Fadilah Mukhlis Mukhlis Mukhtar Mukhtar Mulia Mulia Mulia Mutia Anika mutia anika N. Sri Hartati, N. Sri Nabilah, Rezi Nada Nafion Nadia Nazri, Laila Mardhiyah Nisa Afifah Novitasari, Yuliana Diyah Nur Ayu Ramadanti Nur Helmi Nur Shofiatun Nisa Nur Vaizi Nuraini, Fauzi Eka Nurfadillatun Nisa Wijaya Nurfatihah Z, Zahara Nurhelmi Nurhelmi Nurul Pratiwi Nurul Rahmi Pratama, Chelsylia Dara Pratama, Sandi Fransisco Prima, Rika Putri Rachma Auliya Putri, Aulia Devani Putri, Cici Adelia Putri, Irma Leilani Putri, Irma Leilani Eka Putri, Isna Aryunita Putri Putri, Rani Dwi Suci Putri, Santi Diana Quratul Akyuni Rachmayati, Rike Rahmadhani Fitri Rahmat Afif Rahmatul Huda Asra Rahmawati Darussyamsu Rahmawita Rahmawita Rahmawita Rahmawita Rahmi Holinesti Rahmi, Elva Ramadanti, Nur Ayu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Ratih Rahayu Relsas Yogica, Relsas Resti Desmayanti Rezi Nabilah Rhavy Ferdyan Rhini Febrianti Rinti Mutiara Sari Riri Apriyanti Riri Apriyanti S. Syamsurizal safitri, fira Salmi Halen Salsabilla, Vishtari Samsuriani Siregar Santosa, Tomi Apra Sari, Rinti Mutiara Satria, Rijal Selaras, Ganda Hijrah Shinta Sari Maria Silviana Okwisan Sirwati, Fadila Sisca Alicia Farma Sisri Yandila Siti Aisyah Siti Helmyati Sity Sarroh SOLICHATUN SOLICHATUN Sri Okta Handayani Suhaimi . Sulistiani Sulistiani, Sulistiani Syakirah binti Samsudin Syifa Kamila Namidya Tomi Apra Santosa Tomi Apra Santosa Santosa Valofi, Nagra Aulia Vauzia, Vauzia Vestimarta, Aldi Wahyuda Violita Violita Violita Violita Violita Viona, Alda Wahyuni Wahyuni wahyuni wahyuni Wahyuni, Resma Widya Ruchi Wilna Sari Winardi, Fhuji Wirda Taufik Wulandari, Tesya Yatnita Parama Cita Yolanda Ruhul Azomi Yosi Laila Rahmi Yulita, Nelfi Yuni Ahda Yuni Ahda Yuni Ahda Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zuhratul Mardiyah Amir Amir Zulyusri Zulyusri Zulzusri, Zulzusri