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All Journal TABULARASA Biosfer: Jurnal Tadris Biologi JURNAL PENELITIAN SAINTIKA Journal of Tropical Biodiversity and Biotechnology Jurnal Pendidikan Biologi Assimilation: Indonesian Journal of Biology Education Bioedukasi: Jurnal Pendidikan Biologi JURNAL PENDIDIKAN TAMBUSAI Bioedusiana: Jurnal Pendidikan Biologi Jurnal Perspektif Social, Humanities, and Educational Studies (SHEs): Conference Series Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) Jurnal Darma Agung JURNAL BIOSHELL Bioeduca : Journal of Biology Education Bioscientist : Jurnal Ilmiah Biologi Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Jurnal Ilmu Pendidikan Indonesia Meta: Journal of Science and Technological Education
Fauziyah Harahap
Department of Biology Education, Post Grduate, State University of Medan, Medan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Pengaruh IAA dan Urutan Daun Terhadap Pertumbuhan Tunas Tanaman Anggrek Cattleya sp. Lubis, Anita Agustina; Harahap, Fauziyah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12996

Abstract

Orchids are ornamental plants that have high aesthetic value and beauty. Many human activities use this plant, including weddings, Christmas, New Year and birthdays. Orchid propagation can be done conventionally, but it requires a large area of land and takes a long time to grow. However, it can also be multiplied using tissue culture. Plant tissue culture techniques have better prospects than conventional propagation because tissue culture can produce large numbers of new plants without being influenced by time or season. This research aims to determine the effect of IAA and leaf order on shoot growth of Cattleya sp orchid plants. This type of research is experimental research with a Factorial Completely Randomized Design (CRD) with 12 treatments and 3 replications. There are 2 factors in this study, namely factor I PGR IAA (0, 0.5 mg/l, 1 mg/l, and 1.5 mg/l) and factor II Leaf order (1st leaf, 2nd leaf, and 3rd leaf). This research is a quantitative descriptive study with the parameters of the time of shoot emergence and using 2-way Statistics with Analysis of Variance (ANOVA) with the parameters of number of shoots, number of leaves (strands), and plantlet height. From the research results, it was found that the administration of IAA (Indole Acetic Acid) and the order of the leaves did not have a real effect on the number of shoots, number of leaves (strands) and plantlet height. However, the best treatment is the I0.5D2 treatment (IAA 0.5 mg/l and 2nd leaf).
Induksi Pertumbuhan Tunas Tanaman Manggis (Garcinia mangostana L.) Secara In Vitro Asri, Elita; Harahap, Fauziyah; Hasairin, Ashar
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13681

Abstract

This study aims to determine the effect of plant growth regulators and seed cutting patterns on the induction of mangosteen shoot growth. The research was conducted at the YAHDI Tissue Culture Laboratory in Medan, North Sumatra, Indonesia, in 20245. The method used in this study was a Completely Randomized Design (CRD) with two factors: BAP concentration and seed cutting pattern. The data collected were analyzed using statistical analysis of variance (ANOVA) with Duncan's Multiple Range Test (DMRT) at a 5% significance level using SPSS 26. The results of the study show that the interaction between BAP concentration and seed cutting pattern significantly affected the time of shoot emergence, the number of leaves, and the shoot height. The best shoot emergence time was obtained with a BAP treatment of 4 mg/L using whole seed explants, which occurred at 1 MST (Month After Sowing). A BAP concentration of 2 mg/L with whole seed explants produced the highest number of leaves, with 8 leaves. The highest number of shoots was obtained from explants planted in a medium with 6 mg/L BAP, which resulted in 5.67 shoots. Whole seeds planted in a medium with 0 mg/L BAP produced the tallest plants, measuring 8.9 cm. The combination of BAP concentration and seed cutting pattern did not significantly affect the number of roots.
Development of Tissue Culture Textbook Based on Project Based-Learning to Improve Science Process Skills of Biology Department Students Universitas Negeri Medan Harahap, Salamah; Harahap, Fauziyah; Hasanah, Dwi; Sembiring, Gres Anjelia; Panggabean, Syakilah Kurnia Fahmi; Nadhilla, Annisa; Suriani, Cicik; Edi, Syahmi; Daulae, Abdul Hakim
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13786

Abstract

The research on the development of this textbook was carried out based on the results of the analysis of student needs, namely that there are no teaching materials that can improve students' science process skills in the Tissue Culture course at the Department of Biology, State University of Medan. This textbook is compiled based on the learning syntax of Project-Based Learning (PjBL). This study aims to develop a Project-Based Learning-based tissue culture textbook to improve students' science process skills. This research method uses the Research and Development (R&D) with Thiagarajan's 4D (Define, Design, Develop, and Disseminate). The population of this study was students of the Biology Department of Universitas Negeri Medan with a sample of 47 people who were given a PjBL-based tissue culture textbook. The results showed that (1) the textbooks have been tested for feasibility by material experts (87,73) (2) instructional design experts (92) with a very feasible category, (3) the response of tissue culture lecturers (92) and (4) students based on individual tests (95.1), small group tests (93,75), and limited group tests (90.02) with a very good category and (5) the results of the calculation of N-Gain for science process skills were obtained at 0.66 which is in the medium category.
Optimasi Sterilisasi Eksplan Biji Manggis (Garcinia mangostana L.) Menggunakan Natrium Hipoklorit Secara In vitro Harahap, Fauziyah; Asri, Elita; Hasairin, Ashar; Edi, Syahmi; Suriani, Cicik; Daulae, Abdul Hakim; Panggabean, Nurul Huda
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15281

Abstract

This study evaluates the effects of NaOCl concentration and immersion time on contamination levels and viability of in vitro mangosteen seed explants, aiming to identify the optimal sterilization protocol. Conducted at YAHDI Tissue Culture Laboratory, Medan, Indonesia, a Randomized Complete Block Design tested three treatment combinations: (1) 15% and 10% NaOCl for 5 minutes, (2) 20% and 15% NaOCl for 15 minutes, and (3) 30% and 20% NaOCl for 25 minutes. After immersion, explants were rinsed three times with sterile distilled water. Observations spanned 12 MST, with data analyzed using ANOVA and Games-Howell post hoc tests; contamination sources were qualitatively described. Results showed the first combination had the lowest effectiveness, the second was better but suboptimal, while the third significantly delayed contamination and yielded the highest explant survival rate of 86.1%. The third treatment also prolonged the contamination-free period compared to others. Fungal and bacterial contaminants were predominant, highlighting the need for effective disinfectants against both. These findings indicate that the third combination is a promising sterilization protocol for mangosteen seed explants, especially for hard endocarp tissues prone to contamination.
Variasi Waktu Dan Sterilisasi Untuk Anggrek Cattelya sp. Sebelum Penanaman In-Vitro Harahap, Fauziyah; Br Bangun, Edmy Febriani; Suriani, Cicik; Edi, Syahmi; Ningsih, Ayu Putri; Nusyirwan, Nusyirwan
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8081

Abstract

 Penelitian ini bertujuan untuk Untuk mendapatkan teknik sterilisasi yang terbaik untuk eksplan batang anggrek Cattleya sp. muda. Penelitian ini dilakukan di Laboratorium Kultur Jaringan YAHDI Perum Pelabuhan Jl. Lambung No. 8 Tanah 600 Medan Marelan. Rancangan penelitian yang digunakan dalam penelitian ini adalah metode eksperimental laboratorium dalam mencobakan beberapa teknik sterilisasi. Untuk mengetahui teknik sterilisasi yang tepat, penelitian ini menggunakan eksplan lapang anggrek Cattleya Sp. dengan jenis pendekatan deskriptif kuantitatif. Data dianalisis dan ditabulasi menggunakan parameter penelitian hari terkontaminasi, persentase eksplan yang terkontaminasi, persentase eksplan browning, persentase eksplan yang membengkak, persentase eksplan yang membentuk bakal tunas dan persentase eksplan hidup. Hasil penelitian ini menunjukkan bahwa terdapat 2 teknik sterilisasi yang optimal dalam sterilisasi eksplan lapang Anggrek Cattleya Sp. yaitu teknik sterilisasi 2 dan 4. Teknik sterilisasi 2 menggunakan zat sterilan deterjen, akuades steril, Tween-20, benomil 50%, agrimicin, NaOCl 15% 10% dan alcohol 70% kemudian teknik sterilisasi 4 dengan zat sterilan Clorox 1,05%, HgCl 0,02% dan akuades steril merupakan teknik sterilisasi yang tersebut. Hal ini ditunjukkan dengan rendahnya tingkat kontaminasi eksplan dan tampaknya spot-spot hijau pada eksplan dimana eksplan akan mengalami pertumbuhan tunas. Faktor-faktor yang menyebabkan kontaminasi pada eksplan Anggrek Cattleya Sp. adalah kurangnya kehati-hatian praktikan pada saat melakukan sterilisasi eksplan lapang, kontaminasi endogenus yang tinggi dan penggunaan zat steril yang kurang tepat
Validity of Research Based Book on Isolation, Amplification, and Visualization Material for Biotechnology Course in Higher Education Rizka, Chairany; Harahap, Fauziyah; Edi, Syahmi
META: Journal of Science and Technological Education Vol. 1 No. 1 (2022)
Publisher : Aspirasi Masyarakat Intelektual Islam Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biotechnology is a discipline of biology that examines the use of methods or how to utilize living things and its components in terms of increasing the welfare of human existence. Biotechnology courses currently require instructional materials, particularly those pertaining to the application of DNA isolation and amplification. The aim of this study was to determine the level of validity of research based book on isolation, amplification, and visualization material for biotechnology course in higher education. The  parameters  measured were the validity of the research based book from the aspect of material, language, context, and design. This study uses developmental stage that followed Borg and Gall (1989) development model. The results of the validation by experts were obtained using a questionnaire instrument. The research based book on isolation, amplification, and visualization topic passed validation by materials experts, linguists experts, and design experts with scores of 84% (very good), 77% (very good), and 85%. (very good). It can be concluded that research based book on isolation, amplification, and visualization material product is feasible to use for learning in biotechnology course in higher education based on its validity.
Implementasi Discovery Learning dalam Pembelajaran Biologi Materi Struktur dan Fungsi Jaringan Hewan Manik, Manganju; Harahap, Fauziyah
Bioeduca : Journal of Biology Education Vol. 3 No. 1 (2021): Bioeduca : Journal of Biology Education
Publisher : Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/bioeduca.v3i1.6529

Abstract

Penelitian Tindakan Kelas (PTK) ini dilakukan untuk mendapatkan hasil dari Implementasi Discovery Learning terhadap hasil belajar siswa pada materi struktur dan fungsi jaringan hewan di SMA Negeri 3 Medan. Sistem implementasi ini dilakukan dalam dua siklus, masing - masing siklus terdiri dari empat tahapan yaitu: perencanaan, tindakan, pengamatan (observation) dan refleksi. Terdapat 13 Kelas XI MIA sebagai populasi penelitian, di mana siswa kelas XI MIA-8 yang berjumlah 42 orang terdiri dari 20 siswa laki-laki dan 22 siswa perempuan sebagai subjek penelitian ini. Analisis data menggunakan teknik deskriptif kuantitatif. Peningkatan hasil belajar siswa  mengalami perubahan yakni 85,71% pada siklus ke I dan 97,62% pada siklus ke II. Kesimpulan dari  hasil penelitian menunjukkan bahwa adanya peningkatan hasil belajar siswa dan masuk ke dalam kategori tinggi.
Pengembangan Buku Ajar Kultur Jaringan Berbasis Project Based Learning untuk Meningkatkan Keterampilan Proses Sains Mahasiswa Biologi Azzahra, Yolanda Putri; Harahap, Fauziyah; Panggabean, Nurul Huda; Hadinata, Aristo; Suriani, Cicik; Gultom, Tini Rosalia
Bioedusiana: Jurnal Pendidikan Biologi Vol 10, No 1 (2025): Bioedusiana
Publisher : Jurusan Pendidikan Biologi - Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/bioed.v10i1.14000

Abstract

Minimnya kegiatan praktikum dan kurang lengkapnya materi pada buku kultur jaringan di perpustakaan serta tidak terintegrasikannya model pembelajaran yang dapat melatih keterampilan proses sains (KPS) di dalam buku-buku tersebut membuat rata-rata tingkat KPS mahasiswa Biologi di Universitas Negeri Medan tergolong sedang (49,26). Penelitian ini bertujuan untuk mengembangkan buku ajar kultur jaringan berbasis Project Based Learning serta mengetahui tingkat keefektifan buku dalam meningkatkan KPS mahasiswa biologi pada materi kultur jaringan. Penelitian menggunakan model pengembangan 4D. Subjek penelitian ini terdiri dari ahli materi, ahli media dan desain, dosen kultur jaringan dan 47 orang mahasiswa. Instrumen yang digunakan: lembar studi dokumentasi, lembar wawancara, lembar angket analisis kebutuhan, lembar uji validasi, angket respon dosen dan mahasiswa serta tes KPS. Teknik pengumpulan data yang menggunakan studi dokumentasi, wawancara, kuesioner dan tes sedangkan teknik analisis data dilakukan dengan uji N-Gain. Hasil menunjukkan bahwa persentase kelayakan buku berdasarkan penilaian ahli materi 90,26% (sangat layak) dan ahli media desain 98,6% (sangat layak). Respon dosen memperoleh persentase sebesar 89,06% dengan kategori layak. Uji coba perorangan sebesar 91,66%, uji coba kelompok kecil sebesar 89,5% dan uji coba kelompok besar sebesar 90,21%. Ketiganya berada pada kategori sangat layak. Rata-rata N-Gain KPS mahasiswa memperoleh nilai 0,6 dan rata-rata N-Gain KPS per indikator memperoleh nilai 0,65, kedua angka tersebut berada pada kategori sedang. Berdasarkan hasil penelitian tersebut, dapat disimpulkan bahwa  buku ajar berbasis PjBl cukup efektif dalam meningkatkan KPS biologi pada materi kultur jaringan di Universitas Negeri Medan.
Peran Vital Kepemimpinan Himpunan Masyarakat Pakpak (HIMPAK) Dalam Mempertahankan Jati Diri Silima Suak Ujung, Siti Nuraisah A; Amanda, Putri; Harahap, Fauziyah; Hasairin, Ashar
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.13232

Abstract

Penelitian ini mengulas peran krusial kepemimpinan Himpunan Masyarakat Pakpak (HIMPAK) dalam mempertahankan dan memperkuat jati diri Silima Suak sebagai fondasi identitas Suku Pakpak. Metode penelitian yang digunakan adalah pendekatan kualitatif deskriptif. Pengumpulan data dilakukan melalui wawancara terstruktur dengan Ketua Harian sebagai sumber primer, serta studi dokumentasi sebagai sumber sekunder. Data dianalisis melalui tahapan reduksi, penyajian, dan penarikan kesimpulan untuk memperoleh gambaran mendalam tentang peran dan filosofi kepemimpinan HIMPAK dalam konteks budaya Suku Pakpak. HIMPAK memposisikan diri sebagai “rumah bersama” yang secara efektif menjembatani tradisi dan modernitas. Temuan utama menunjukkan bahwa kepemimpinan HIMPAK menerapkan prinsip inklusif dengan memastikan adanya keterwakilan yang adil dari seluruh Silima Suak dalam struktur kepengurusan. Pengambilan keputusan organisasi dijiwai oleh filosofi luhur, yang menuntun pada musyawarah mufakat. Fokus program HIMPAK tidak hanya pada pelestarian seni, budaya, dan adat, tetapi juga pada kaderisasi pemuda untuk kelanjutan tradisi, serta perhatian pada peningkatan kesejahteraan ekonomi masyarakat (melalui IKM dan Koperasi)
The Development of Teaching Material of In Vitro Plant Breeding Based on Scientific Literacy Harahap, Fauziyah; Salsabila, Az-Zahra Shakila
Bioedukasi: Jurnal Pendidikan Biologi Vol 15, No 1 (2022): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v15i1.56103

Abstract

This development research was carried out based on the observations of lecturers and students Department of Biology at Universitas Negeri Medan who stated that the tissue culture course was still quite difficult to learn, and the level of scientific literacy in Indonesia according to PISA was still relatively low. So, the purpose of this development research in general is to improve student scientific literacy, one of which is by developing tissue culture teaching materials. The research method for developing tissue culture teaching materials refers to the Dick & Carey model. However, this research has only reached the stage of designing and conducting formative evaluation. Analysis of the data used in this development research using quantitative descriptive data analysis. The result showed that the development of teaching material of in vitro plant breeding based on scientific literacy with a feasibility level based on material expert validation obtained a percentage of 91% in the very worthy category, learning design expert validation obtained a percentage of 95% in the very worthy category, layout design expert validation obtained a percentage of 99,2% in the very worthy category, response from lecturer of tissue culture course obtained a percentage of 95% in the very worthy category, and student responses obtained a percentage of 87% in the worthy category.
Co-Authors A.A. Ketut Agung Cahyawan W Adelia, Ichlasul Ajat Sudrajat Amanda, Putri Anisa, Cut Anliana, Anliana Anwar, Syakila Apriliani, Indah Rizka Armaniar, Armaniar Aryeni Aryeni, Aryeni Ashar Hasairin Asri, Elita Ayu Putri Ningsih Azzahra, Yolanda Putri Baby Arlita Lubis, Baby Arlita Batubara, Wardah Arijah Binari Manurung, Binari Br Bangun, Edmy Febriani Chairany Rizka CICIK SURIANI Dalimunthe, Rahmadina Damanik, Jesika Febriani Daulae, Abdul Hakim Daulay, Abdul Hakim Dwi Apriliani, Dwi Eko Prasetya, Eko Ely Djulia Eryati Zetkas, Eryati Fachry Abda El Rahman Fajriati, Qisthy Adha Fian Sinaga, Roberi Sepda Fitri Rahmadani, Fitri Fitri, Walida Gultom, Novayanti Hadinata, Aristo Harahap, Salamah Hariyadi, Itra Hasanah, Dwi Hasratuddin . Hasruddin Hendro Pranoto Hengki Frengki Manullang, Hengki Frengki Herbert Sipahutar, Herbert Herbet Sipahutar, Herbet Humairah, Erfiani I Made Nuada, I Made Indrayani, Lisa Indriana, Githa Ivan Lauren, Ivan Kamila, Aryediutami Naura Karinanta Sembiring, Surya Khalisyah, Zhafira Lazuardi Lazuardi Limbong, Forentina Lubis, Anita Agustina Lubis, Khairiza Lubis, Theresia Mahmud Mahmud Manik, Manganju Mardiana Mardiana Mariana Siregar, Mariana Martina Asiati Napitupulu Melva Silitonga Mufti Sudibyo, Mufti Nadhilla, Annisa Nainggolan, Junister Nanda Pratiwi Nasution, Marina Azzahra Natalia Kristiani Lase, Natalia Kristiani Naura Kamila, Aryediutami Nazwari, Aura Nora, Putri Nurlia Zahara Nursamsu Nursamsu Nusyirwan . Nusyirwan Nusyirwan Panggabean, Nurul Huda Panggabean, Syakilah Kurnia Fahmi Parasetya, Eko Pasaribu, Fadilah PERMATA GINTING, PERMATA Pranoto, Hendro Puji Prastowo, Puji Rahayu, Ari Ramayanti Ramlan Silaban Renny Agustina, Renny Rumapea, Lusiana Ruth Susanty, Revi Said . Iskandar Sailana Mira Rangkuti, Sailana Mira Salsabila, Az-Zahra Shakila Samsul Kamal Sari, Elvira Nanda Satriawati Satriawati, Satriawati Sembiring, Gres Anjelia Sembiring, Surya Karinanta Sihombing, Josephine Gloria Margareth Silitonga , Melva Sinuraya, Kenari Br Sipahutar , Herbert Sitompul, Naila Zahwa Sitorus, Henry Pangihutan Situmorang, Berri Sohada, Ummi Sri Rahmadani Harahap, Sri Rahmadani Sudibyo , Mufti Suryani, Cicik Susanty, Revi Ruth Syahmi Edi Syarifuddin Syarifuddin Tambunan, Maria Cintiana Tambunan, Muhammad Iqbal H. Tanjung, Aura Albizia Tarigan, Wenny Pinta Litna Tini Rosalia Gultom, Tini Rosalia Triyusmidarti, Neni Tumiur Gultom Ujung, Siti Nuraisah A Wahida Rahmadani, Wahida Widya, Poppy Andra Yuanita Rachmawati Yusuf, Yusnaeni Zulkifli Matondang Zulpadly Zulpadly, Zulpadly