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PELAYANAN KESEHATAN SAPI BALI DI WILAYAH LINGKAR KAMPUS UNIVERSITAS UDAYANA BUKIT JIMBARAN KECAMATAN KUTA SELATAN KABUPATEN BADUNG I. N. Suarsana; I. K. Suatha; P. Sampurna; I. M. Kardena; I. W. Suardana; I. H. U Utama
Buletin Udayana Mengabdi Vol 15 No 2 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

One of the obstacles that are often faced in livestock raising is a matter of disease. Even sometimes farmerssuffered economic losses and no longer breeding due to the death of livestock. The health service is one ofthe factors that determine the success of a farm. The aim of this implemented service to improve health ofbali cattle by through health services which include spraying, provision of vitamins and treatment of cattle atUniversity of Udayana Campus around area Jimbaran Hill. Results of community service activities on cattleat University of Udayana Campus around area Jimbaran Hill, showed a positive response and a highenthusiasm of livestock farmers, that shown with the large number of cattle (37 cattle ) which can be given alivestock health services. The number and type of service animal on the farm is dominated by provision ofvitamins as much as 32 cattle (86.48%), spraying action of 30 cattle (81.08%), worm medication of 7 cattle(18.92%), and administration of antibiotics of 6 cattle (16.21%). Breeders appreciate the service activities ofthe Institute for Research and Community Services and hope a sustainable because it was felt useful.
TEKNIK GRAFTING DAN BUDIDAYA HORTIKULTURA PADA KELOMPOK TANI MODEL KAWASAN RUMAH PANGAN LESTARI DI DESA GETASAN- KABUPATEN BADUNG I N. Suarsana; I. W. Suardana; I K.M. Budiasa; I P. Sudiarta; I G. Wijana
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Guna mengurangi penyakit-penyakit pada tanaman tomat maka perlu dilakukan penyambungan dengan tanaman terong sehingga hortikultura tersebut tahan terhadap penyakit. Tujuan kegiatan ini adalah meningkatkan pengatahuan grafting (penyambungan) dan budidaya hortikultura dengan sistim polibag. Metode yang digunakan adalah pendekatan dengan advokasi (penyuluhan) dan metode edukasi (pelatihan) grafting (teknik penyambungan). Hasil kegiatan menunjukkan bahwa kelompok tani Model Kawasan Rumah Pangan Lestari (MKRPL) mampu melakukan teknik budidaya hortikultura mulai pemilihan bibit dan persyaratanya, syarat pertumbuhan, teknik penyediaan media tanam (tanah dan pupuk), penyemaian bibit, dan cara bertanam dengan sistim polibag. Kelompok MKRPL mampu menguasai teknik grafting (penyambungan) dan melakukan penyambungan tanaman terong pada bagian bawah dan tanaman tomat pada bagian atas dengan hasil baik. Dapat disimpulkan bahwa kelompok tani Model Kawasan Rumah Pangan Lestari (MKRPL) mampu melakukan grafting (penyambungan) dan budidaya hortikultura dengan sistim polibag dan dapat memanfaatkan lahan kosong untuk diisi dengan tanaman hortikultura sehingga lebih produktif.
PELAYANAN KESEHATAN SAPI DI BANJAR SAMUAN KAWAN DAN SAMUAN KANGIN, DESA CARANGSARI, KECAMATAN PETANG, KABUPATEN BADUNG I W AYAN SUARDANA
Buletin Udayana Mengabdi Vol 13 No 1 (2014): Vol 13, No 1, April 2014
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Animal health service is one of key factors to be successful in animal breeding. This program include curativeaction for animal illness, sanitation program and vaccination as a preventive program. Carangsari village especiallySamuan Kawan and Samuan Kangin Sub-villages is potential as one of animal production centrals at BadungRegency, so that the animal health service program is potentially conducted at both places. Results of programshowed the responds of society especially for cattle farmers were very interesting that were identified by so manyanimals were treated by this program i.e. 34 heads (89,5%) treated by vitamins, 21 heads (55,3%) treated byspraying, 7 heads (18,4%) treated by anthelmentics, and 4 heads (10,5%) treated by curative actions. These resultsindicated the animals health service program is helpfully to respond the basic need of animal farmers.Key words : Animals health services, SE, vaccination, spraying, cattle
Identifikasi Escherichia coli O157:H7 serta Deteksi Gen Shiga Like Toxin 1 dan 2 Asal Feses Hewan, Daging, dan Feses Manusia I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Escherichia coli O157:H7 with the ability to produce shiga-like toxin was isolated from beef, cattle,chicken, and human feces. Due to its importance to human health, it is necessary to identify the genesencoding the production of shiga-like toxin, stx1 and stx2 respectively to further understand the pathogenesis.Isolation of E. coli was done on Eosin Methylene Blue Agar (EMBA), followed by identification on SorbitolMacConkey Agar (SMAC), latex agglutination test, and H7 antiserum test, respectivelly. The existence ofgenes stx1 and stx2 in E. coli O157:H7 was confirmed molecularly using PCR method with specific primersLP 30/31 and LP 43/44, Stx2 (F)/Stx2 (R) respectively. Escherichia coli O157:H7 was isolated from 22 outof 344 samples (6,4%). Some isolates showed gene stx1 and stx2 was detected in two isolates as indicatedby a 384 bp band (stx1 gene), 584 bp and 1588 bp bands (stx2 gene) respectivelly. The results indicatedthat local isolates E. coli O157:H7 are potential as a zoonoses agent.
Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali sebagai Kandidat Biopreservatif ISOLATION AND IDENTIFICATION OF ACID LACTIC BACTERIA FROM BALI CATTLE’S GASTRIC FLUID AS A POTENTIAL CANDIDATE OF BIOPRESERVATIVE I Wayan Suardana; I Nyoman Suarsana; I Nengah Sujaya; Komang Gede Wiryawan
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate and identify of lactic acid bacteria originated from gastric fluid of bali cattle, and to determine their potential as the candidates of biopreservative. Lactic acid bacteria were isolated by culturing the gastric fluid of bali cattle in de Mann, Rogosa, Sharpe (MRS) medium; screening the bacteria, and identification of bacteria species by Analytical Profile Index (API) 50 CHL Kit. The results showed that, the new species of lactic acid bacteria were isolated and identified as Lactococcus lactis spp lactis 1 (SR21 isolate) and Lactobacillus brevis 1 (SR54 isolate) that have broad spectrum antimicrobial activities. It is clear from this study that a potential lactic acid bacteria producing antimicrobial agent can be isolated from the gastric fluid of bali cattle.
KAJIAN TENTANG BERAT RELATIF BEBERAPA ORGAN VISERAL ITIK BALI STUDY OF THE RELATIVE WEIGHT OF SEVERAL VISCERAL ORGANS OF BALI DUCKS Ida Bagus Swacita; I Wayan Suardana
Jurnal Veteriner Vol 7 No 4 (2006)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pada dasarnya hasil pemotongan ternak itik dapat dikategorikan menjadi dua komponen yaitu komponen karkas dan non-karkas
Bakteri Asam Laktat Isolat 18A (Lactococcus lactis ssp lactis 1) Asal Kolon Sapi Bali Berpotensi sebagai Probiotik (LACTID ACID BACTERIA ISOLATES 18A (Lactococcus lactis ssp lactis 1) COLONIC ORIGIN BALI CATTLE POTENTIAL AS PROBIOTICS) I Wayan Suardana; Yan Ramona; Sri Wahyuni
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The main aim of this study was to determine the potency of lactic acid bacteria (LAB) isolate 18A(Lactococcuslactisssplactis 1),isolated from the colon of Bali cattle to be used as a probiotic candidate. The study was started by confirmation tests of the isolate, including test for growth response in De Man Rogosa and Sharpe(MRS) brothmedium, test on catalase production, and Gram staining.Its probiotic potencywas tested by growing theisolate in MRS broth medium in low pH conditions (pH 2.3 and 4) and in MRS medium supplemented with various concentrations of sodium deoxycholatet (NaDC) (0.2 mM, 0.4 mM, and 0.6 mM). The results showed that isolate 18A was able to grow well in De Man Rogosa and Sharpebrothmediumunder anaerobic condition. In addition, the isolate was catalase negative and Gram positive, indicating that this isolate was confirmed as an LAB isolate. The isolate was resistant to low pH conditions (up to pH 2) and to high concentration of NaDC (up to 0.6 mM), indicating that this isolate has potential to be developed as a probiotic candidate. Further tests are still required to confirm its use asprobiotic. ABSTRAK Bakteri asam laktat (BAL) merupakan salah satu kelompok mikroorganisme yang memiliki peranan penting dalam menjaga kesehatan saluran pencernaan pada hewan atau manusia. Tujuan penelitian ini adalah untuk mengetahui potensi bakteri asam laktat isolat BAL 18A (Lactococcus lactis ssp lactis 1)yang diisolasi dari kolon sapi bali sebagai kandidat probiotik. Penelitian diawali dengan tahapan konfirmasi isolat 18A melalui beberapa uji, seperti uji penumbuhan pada De Man Rogosa and Sharpebroth, uji katalase, dan pewarnaan Gram. Potensinya sebagai probiotik diuji dengan melakukan pengujian terhadap pH rendah (pH 2, 3, dan 4) dan natrium deoksikolat (NaDC) dengan konsentrasi 0,2 mM, 0,4 mM, dan 0,6 mM. Hasil penelitian menunjukkan bahwa isolat BAL 18A mampu tumbuh dengan baik pada medium De Man Rogosa and Sharpebroth dalam suasana anaerob, katalase negatif, dan Gram positif,. Hasil ini mengindikasikan bahwa isolat 18A ini adalah isolat BAL. Dalam uji ketahanan terhadap pH rendah dan NaDC, isolat ini mampu bertahan hidup pada medium pH 2 dan NaDC pada konsentrasi sampai 0,6 mM. Simpulan penelitian ini adalah isolat BAL 18A berpotensi dikembangkan sebagai kandidat probiotik.
Penentuan Marka Genetik Escherichia coli O157:H7 Asal Hewan dan Manusia dengan Metode Random Amplified Polymorphic DNA (GENETIC MARKERS IDENTIFICATION OF ESCHERICHIA COLI O157:H7 ORIGINATED FROM ANIMALS AND HUMAN BY USING RANDOM AMPLIFIED POLYMORPHIC DNA I Wayan Suardana; Dyah Ayu Widiasih; Komang Januartha Putra Pinatih
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The use of random amplified polymorphic DNA (RAPD) as a method to identify a genetic markerof bacteria is widely used by researcher. This method is known as a simple, faster, and reliabletechnicque. This study is to find out the aplication of RAPD method in order to identify specific markersof E. coli O157:H7 as a zoonotic agent. The study began by cultivating of 20 isolates of E. coli O157:H7colected by previous study that consist of 2 isolates originated from cattle feces, 2 isolates originatedfrom beef, 2 isolates originated from chicken feces, 2 isolates originated from healthy human and 11isolates originated from unhealthy human (human with kidney failure). All isolates were confirmed byculturing on selective medium sorbitol MacConkey agar (SMAC). Confirmation were followed by testingon O157 latex aglutination, and finally by testing on H7 antiserum. RAPD method as molecularanalysis was performed using decamer primers mixture OPA-01, OPA-02, OPA-03, and OPA-04.Results of study showed both bands 1721 and 700 bp are specifically to differentiate of isolatesoriginated from cases of healthy and unhealthy human. On the other hand, bands with position 1721 bp,300 bp, and 250 bp indicate the isolates originated from unhealthy human, healthy human and chicken,respectively. Isolates from beef are characterized by both bands 1400 and 429 bp, and the isolates fromcattle feces are identified by band with position 342 bp. The specific bands are considered as markers inorder to know the source of E. coli O157:H7 fastly.
Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES) I Wayan Suardana; I Nengah Sujaya; Wayan Tunas Artama
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A Shiga-like toxin producing Escherichia coli O157:H7 was detected in faecal samples of cattle, andhuman as well as in beef. The performance of agent indicated that it has been identified as harmful andoften life-threatening zoonotic agent. It is therefore important to analysed the genetic characteristic ofShiga toxin Escherichia coli (STEC) and to develop a diagnostic probe in order  to optimalized of diagnostictest  for the agent. The  study was started by amplifiying  stx2 gene, purifying of PCR product, sequencingof stx2 gene, analyzing  of phylogenetic tree, and finally  by analyzing   of  diagnostic  probe candidate.Homology study showed that the genetic sequence of the local isolate of  E. coli O157:H7 i.e SM25(1)isolated from cattle feces has  a genetic and fuctional similarity with  the control isolate i.e E. coli O157:H7ATCC 43894 originated from human.  Further study showed that a probe with  foreward primer  sequanceof 5’-AATTTATATGTGGCCGGGTTC-‘3 which were respectively designed as a PFS and PRS 176 bp product.Appeared to be potential candidate of diagnostic probe for the agent.
Botulismus pada Manusia (BOTULISM IN HUMAN) I Wayan Suardana
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Botulismus pada Manusia   (BOTULISM IN HUMAN)
Co-Authors Abdul Manan Achjar, Komang Ayu Henny Adirinata, I Komang Pasek Agus Sri Lestari Amrulloh, Muhammad Faqih Anak Agung Gde Bagus Udayana Andi Isma Lestari Amin, Andi Isma Ardika, I Gusti Ngurah Putu Ari Yuniastuti Aribaten, Ni Nengah Zinnia Arimbawa, I Gede Artayani, Ida Ayu Gede Asyauqi Ilham Perdana, Asyauqi Ilham Bambang Sumiarto Bq Nurlita Anugrah BUDI SETIADI DARYONO Budi Setiadi Dayono Chandra Yowani D. A. Widiasih Denny Widaya Lukman Devi Dwi Jayanti Dewa Gede Agung Widyadnyana Dewa Putu Oka Prasiasa Dewi, Ni Putu Diah Trisna Dewintasari, Ni Nyoman Paramitha Dwi Lestari Dyah Ayu Widiasih Dyah Ayu Widiasih Dyah Ayu Widiasih Eka Putri Suryantari Emmanuella Felice’anna Dije Karisoh Febrianti, Andri Nurdiana Franciska, Juliana Gama, I Ketut Gama, Ketut Gede Arimbawa, I Made Hana Kristal Alamanda Septiara Harini, I Gusti Ayu Hartati, Ni Nyoman Henny Achjar, Komang Ayu I Dewa Made Sukrama I G. Wijana I Gede Gian Saputra, I Gede I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Putu Bagus Sasrawan Mananda I Ketut Adi Sugita I Ketut Mangku Budiasa I Ketut Muka I Ketut Suada I Ketut Suardana I Ketut Suatha I Komang Gede Wiryawan I Made Adikampana, I Made I Made Kardena I Made Sukada I Made Sukarja I Made Walesa Putra I Nengah Kerta Besung I Nengah Sujaya I Nengah Wirakesuma, I Nengah I Nyoman Ariana I Nyoman Suarsana I Nyoman Sudiarta I Nyoman Sukma Arida I Nyoman, suardina I Putu Sampurna I Putu Sudiarta I Wayan Adnyana I Wayan Mustika I Wayan Seriyoga Parta I. H. U Utama Ida Bagus Ngurah Swacita Iga Prassetyo Adji, Iga Prassetyo INDAYATI LANYA Iwan Harjono Utama Karuni, Ni Kadek Khamid Yusuf Baehaqi, Khamid Yusuf Komang Januartha Putra Pinatih Kondra, I Wayan Korbinianus Feribertus Rinca Kumalasari, Ni Putu Putri Laba, I Nyoman MAS DJOKO RUDYANTO Meitisrilatifatulain Fitriadewi Mariana Mertanadi, I Made Michael Haryadi Wibowo Mita Ekamelinda Ngurah, I Gusti Ketut Gede Ngurah, IGK Gede Ni Kadek Lyming Lestari Ni Ketut Suwiti Ni Luh Sustiawati Ni Luh Watiniasih Ni Made Ariani Ni Made Ayu Aryati Dinarini Ni Made Ruastiti Ni Putu Ratna Sari Nuria Fitrianti Putri Nyoman Dewi Pebryani NYOMAN SEMADI ANTARA Oktivia Chandra Mustika, Oktivia Chandra P. Sampurna Padmi, Luh Sri Anggayoni Julia Piartrini, Saroyeni Pitriyantini, Putu Eka Pramesti, Kadek Diah Pratiwi, Ida Ayu Windhari Kusuma Putra, I Kadek Aldi Margareta Perdana Putu Ayu Sisyawati Putriningsih Putu Cahaya Semesta Putu Januari Ratna Apsari Putri R Susanti Raharjo, Anis Rasdianah Rasdianah Remawa, Anak Agung Gede Rai Reny Navtalia Sinlae Rian Ka Praja Ribek, Nyoman Richard Christian Daud Ruspawan, Dewa Made Ruta, I Made Satriawati, Ni Nyoman Ayu Siti Helmyati Sri Anggreni Lindawati Sri Wahyuni Suarya Putra, I Nyoman Agus Sudiantara, I Ketut Sudiantara, Ketut Suharsono, Hamong Sukoco, Hendro Sunatha Putra, Agus Surya, I Kadek Adi Tjokorda Sari Nindhia Totton, Mary Louise Victor Bangun Mulia Wahyu Hananto, Wahyu Wayan Tunas Artama Wayan Tunas Artama Widya Asmara Wilantari, Ni Nyoman Ayu Wulandari, Andi Dewi Wulandari, Kadek Dina Yan Ramona Yuli Darmawan, Yuli Yunita Sri Hastuti, Yunita Sri