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PERCENTAGE OF CD3+ T LYMPHOCYTES EXPRESSING IFN-γ AFTER CFP-10 STIMULATION (Persentase Limfosit T-CD3+ yang Mengekspresikan Interferon Gamma Setelah Stimulasi Antigen CFP-10) Yulia Nadar Indrasari; Betty Agustina Tambunan; Jusak Nugraha; Fransiska Sri Oetami
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1181

Abstract

Tuberkulosis (TB) merupakan penyakit infeksi menular, disebabkan oleh Mycobacterium tuberculosis. Respons imun adaptif yangdiperantarai oleh limfosit T berperan sangat penting dalam menyingkirkan bakteri intraseluler. Hasilan sitokin IFN-γ merupakanmekanisme efektor utama dari limfosit T. Pengembangan vaksin yang efektif dalam melawan infeksi TB mempertimbangkan faktor yangmengatur hasilan IFN-γ. CFP-10 merupakan antigen yang disekresikan oleh Mycobacterium tuberculosis. Antigen ini dikenal sebagaikomponen vaksin potensial untuk TB. Tujuan penelitian ini adalah membandingkan respons imun seluler yaitu persentase limfosit T-CD3+yang mengekspresikan IFN-γ setelah dirangsang antigen CFP-10 di pasien TB paru kasus baru, TB laten dan orang sehat. Penelitianini menggunakan desain eksperimen murni di laboratorium secara in vitro pada kultur PBMC pasien TB paru kasus baru, TB latendan orang sehat. Subjek penelitian adalah 8 pasien TB paru kasus baru, 7 TB laten dan 7 orang sehat di RS Khusus Paru Surabaya.Pemeriksaan persentase limfosit T-CD3+ yang mengekspresikan IFN-γ dengan metode Flow cytometry (BD FACSCalibur). Hasil dianalisisdengan Kruskal-Wallis atau ANOVA satu arah. Rerata persentase limfosit T-CD3+ yang mengekspresikan IFN-γ di TB paru kasus barusetelah stimulasi antigen CFP-10 (4,36%) lebih tinggi daripada sebelum stimulasi (3,50%) (nilai P=0,015). Rerata persentase limfositT-CD3+ yang mengekspresikan IFN-γ di TB laten setelah stimulasi antigen CFP-10 (3,96%) lebih tinggi dibandingkan sebelum stimulasi(2,50%) tetapi tidak bermakna (nilai P=0,367). Rerata persentase limfosit T- CD3+ yang mengekspresikan IFN-γ di orang sehat setelahstimulasi (1,66%) lebih rendah daripada sebelum stimulasi (2,89%) tetapi tidak bermakna (nilai P=0,199). Perubahan persentaselimfosit T-CD3+ yang mengekspresikan IFN-γ setelah stimulasi antigen CFP-10 antarkelompok tidak berbeda bermakna (nilai P=0,143).Berdasarkan hasil telitian ini dapat disimpulkan bahwa terdapat peningkatan persentase limfosit T-CD3+ yang mengekspresikan IFN-γdi TB paru kasus baru setelah stimulasi antigen CFP-10. Hal ini menunjukkan limfosit T-CD3+ yang mengekspresikan IFN-γ berperandalam perlindungan terhadap infeksi TB paru.
Evaluation of the Progressivity Parameters of Chronic Kidney Disease after Branched-Chain Amino Acid Supplementation in Children Esthy Poespitaningtyas; Roedi Irawan; Ninik Asmaningsih Soemyarso; Jusak Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1467

Abstract

Chronic Kidney Disease (CKD) is not an uncommon issuein children. Chronic kidney disease is the abnormality ofstructure or function of the kidney that occurs for more than three months. The presence of a longitudinal decline inGlomerulus Filtration Rate (GFR), proteinuria, and hypertension Are the characteristics of CKD. One of the recommendationsof nutritional supplementation as the prevention of CKD is by the administration of oral Branched-Chain Amino Acid (BCAA).To date, there has been no research to analyze the effects of the BCAA on children with stage 2-4CKD. This study aimed toanalyze the effect of BCAA in inhibiting the progressivity of stage 2-4 CKD in children and improving nutritional status.Astudy with randomized pre-post test controlled trial design was performed in the Outpatient Clinic of Pediatric Nephrologyin Dr. Soetomo Hospital with stage 2-4CKD. The subjects were divided into two groups, such as the BCAA and placebo, andwere monitored for eight weeks to be evaluated the GFR, albumin, proteinuria, blood pressure, and nutritional status.Sixteen children with stage 2-4 CKD dominated by 71.4% of male patients were enrolled in this study. The mean age was 12.5(SD 2.90) years. Approximately 50% (p=0.767) stage 2 chronic kidney, 50% (p=1.000) moderate malnutrition, and 64.28%(p=1.000) short stature were found, with nephrotic syndrome as the most common underlying cause of CKD (p=0.149). InBCAA group, decrease of GFR -5.08±7.13 (p=0.055), increase of serum albumin 0.20±0.23 (p=0.062), decrease of deltasystole -11.57±15.08 (p=0.565) and diastole -4.85±16.25 (p=0.708), weight loss -0.07±1.01 (p=0.828), an increase of height0.14±0.24 (p=0.771), and a decrease in BMI -0.03±0.74 (p=0.389) were reported. It was concluded that branched-chainamino acid (leucine, isoleucine, and valine) supplementation did not provide a significant effect to inhibit progressivity ofstage 2-4CKD in children and improvement of nutritional status.
PEMETAAN EPITOP DAN APLIKASI KLINISNYA Jusak Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1168

Abstract

Epitope mapping is one of the important findings in immunoIogy. The idea of systematic epitope mapping was first described byGeysen et al (Geysen et al., 1987a,b). This technic is further developed together with other important findings such as monoclonalantibody production, DNA recombinant, peptide synthesis and phage display of peptide or protein. The usage of this technic is to know theexact binding site of antigen with antibody or T cell receptor, and can be used as the basic information to design a vaccine or diagnostictools. The term epitope can be further classified as functional epitope, structural epitope, binding epitope, protective epitope, heavyinfection epitope, neutralization epitope etc. In this article will be reviewed topics about epitope: B and T cell epitope mapping technicsusing synthetic pin and its application.
ST2 DI INFARK MIOKARD AKUT Hery Priyanto; Jusak Nugraha; SP Edijanto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.478

Abstract

Acute Myocardial Infarction (AMI) is a major problem threatening Indonesian inhabitants as well as in many countries. Each year AMI patients are increasing. ST2 is a novel cardiac marker for the diagnosis and prognosis of acute myocardial infarction. The purpose of this study was to know and find the diagnostic value of ST2 serum in patients with AMI. This cross sectional study was conducted on 46 patients who had chest pain as the main complaint in the Emergency Department, Dr. Soetomo Hospital, Surabaya between April 20 to July 20, 2013. The sera were examined for ST2, cTn-T, the diagnostic value was determined using the acute myocardial infarction clinical criteria according to the Universal Definition of Myocardial Infarction (2007) as the gold standard. The results of ST2 serum levels measurement showed a sensitivity of 83% and specificity of 70% at a cut-off value of ST2 16.64 ng/mL and AUC 0.878, p=0.000. The concordance examination between using cTn-T levels and ST2 was 48.1%. A significanct correlation was obtained with a correlation coefficient r=0.489, p=0.001 between the levels of cTn-T and ST2. Based on this study, the ST2 serum levels can be used for screeningto aid the diagnosis of acute myocardial infarction. However, there is a weak correlation and concordance between cTn-T with ST2. Thus, the researchers need a further study to determine the diagnostic value of ST2 for the detection of acute myocardial infarction.
IDENTIFIKASI KRIPTOSPORIDIOSIS DI PASIEN ANAK HIV DENGAN DIARE KRONIS DI RUANG GASTRO ANAK Jusak Nugraha; Febtarini Rahmawati; Dominicus Husada
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 1 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i1.349

Abstract

Based on the results of overseas researchers, Cryptosporidiosis occurs in immunosuppressive cases with chronic diarrhoea. In this study the researchers would like to know exactly whether that Cryptosporidiosis occurs also in paediatric HIV patients. The latest data show that the incidence of opportunistic infection is characterized by persistent diarrhoea and severe malnutrition as a complication of the paediatric HIV-infected patients is increasing. The objects of the research were fifteen paediatric HIV-infected patients which treated at the Paediatric Gastro Ward of Dr. Soetomo Hospital Surabaya due to persistent diarrhoea. Paediatric patients were less than five years old, suffered persistent diarrhoea more than two weeks with severe malnutrition. Stool specimens were transported using 10% formalin. The stool suspension was filtered, and distilled water was added followed by centrifugation (sedimentation method). The precipitate material was placed on a glass object and dried, and then fixed by methanol and stained with Acid Fast Staining and trichrome staining. The protozoa Cryptosporidium spp. was observed under a binocular microscope with 100× magnification (immersion oil) objective. The result was confirmed as positive if a red spherical or oval formation of oocyste of 4–6 micron appeared. Sixty percent of the 15 paediatric HIV-infected patients with chronic diarrhoea showed positive cryptosporidiosis. Cryptosporidiosis is one of the opportunistic infections resulting in chronic diarrhoea in paediatric HIV-infected patients. The results of the present research indicate that the enteric parasite Cryptosporidium spp. was the main cause of persistent diarrhoea in paediatric HIV-infected patients
DIFFERENCES OF PLASMA INTERLEUKIN-6 AND TUMOR NECROSIS FACTOR-α LEVELS IN HEALTHY PEOPLE, RIFAMPICIN RESISTANT AND SENSITIVE PULMONARY TUBERCULOSIS PATIENTS Wahyu Setiani Wibowo; Jusak Nugraha; Soedarsono Soedarsono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1452

Abstract

Increased tuberculosis in the world is caused by increased HIV-infected and antituberculous drugs (rifampicin) resistant individuals. IL-6 and TNF-α play an essential role in explaining the different degrees of inflammation in Rifampicin Resistant (RR) and Rifampicin Sensitive (RS) pulmonary tuberculosis patients, and healthy people. The research aimed to analyze the differences in plasma IL-6 and TNF-α levels in healthy people, Rifampicin Resistant (RR), and Rifampicin Sensitive (RS) pulmonary tuberculosis patients. A cross-sectional study was conducted from July-September 2017. Thirty-nine subjects were classified into RR pulmonary tuberculosis (n=15), RS pulmonary tuberculosis (n=12) based on GeneXpert examination and treated by antituberculous drugs ≤ 1 month, and healthy people (n=12) based on AFB results, Thorax X-ray, and tuberculin tests. IL-6 and TNF-α were done in all subjects using ELISA U-CyTech®(Biosciences, Inc.). Anova analyzed differences of IL-6 and TNF-α levels between groups. The mean IL-6 levels (pg/mL) in RR and RS pulmonary tuberculosis patients, and healthy people were 54.56±59.13, 27.05±37.04, 4.42±2.83, respectively. The mean TNF-α levels (pg/mL) in RR and RS pulmonary tuberculosis patients, and healthy people were 263.54±327.58, 250.25±314.20, 9.04±5.89, respectively. The mean differences between  IL-6 and TNF-α levels (pg/mL) between RR pulmonary tuberculosis patients and healthy people were 50.14±15.29 (p<0.05) and 254.59±8460 (p<0.05). Significant differences of mean IL-6 and TNF-α levels were found between RR pulmonary tuberculosis patients and healthy people.
Correlation of Serum Interleukin-6, TNF-α, Procalcitonin and Leukocyte Count in Patients with Suspected Sepsis Erfina Lim; Jusak Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1463

Abstract

Sepsis is a cause of non-cardiac death in the hospital. Early and rapid diagnosis of septic patients is a challenge toincrease the expectancy of life. IL-6 and TNF-α are groups of pro inflammatory cytokines that initiate an initial inflammatoryresponse. Procalcitonin is a specific marker of bacterial infection. This study aimed to analyze the correlation of serumcytokine IL-6, TNF-α, procalcitonin and leukocyte count in suspected sepsis patients. This was a cross-sectionalobservational study consisting of 45 patients with suspected sepsis with procalcitonin level > 0.5 ng/mL. Procalcitonin levelwas measured with Enzyme-Linked Fluorescent Assay (ELFA) (VIDAS), IL-6 and TNF-α levels were measured with theU-CyTech Human Elisa kit (Bioscience, INC) and leukocyte counts were measured with SYSMEX-XN 1000. It was found in thisstudy that IL-6 levels ranged in 0 pg/mL – 73.29 ng/mL (mean 29.43 ng/mL), TNF-α levels were 0 pg/mL – 390.5 pg/mL (mean27.62 pg/mL), and the mean value of leukocytes was 20,139/μL. There was no correlation between leukocyte counts with IL-6(p=0.798 and r=0.040), TNF-α (p=0.304 and r = -0.160), and procalcitonin (p=0.323 and r = 0.154). There was no correlationbetween IL-6 levels with TNF-α levels (p=0.871 and r = -0.025), and procalcitonin levels (p = 0.466 and r = 0.112). There wasa weak negative correlation between TNF-α level and procalcitonin levels (p=0.006 and r = -0.403) and there was a weaknegative correlation between procalcitonin and TNF-α levels in suspected sepsis patients.
MACROPHAGE AUTOPHAGY IN IMMUNE RESPONSE Jusak Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1164

Abstract

Otofagi adalah mekanisme yang digunakan oleh sel untuk menyerap, membuang dan mendaur ulang sampah. Makrofag dapatberfungsi untuk menangkap, mengonsumsi dan mencerna antigen eksogen, keseluruhan mikroorganisme, partikel yang tidak larut danbahan endogen misalnya: sel inang yang sekarat atau rusak yang dipajankan oleh limfosit. Saat ini makrofag dapat dibagi menjadi duajenis aktivasi: Aktivasi klasik (M1); Aktivasi alternatif (M2) yang memiliki efek berbeda. Aktivitas M1 meningkatkan respons Th1 misalmenyebabkan peradangan, pembunuhan patogen intraselular, DTH (tipe hipersensitivitas tertunda) dan kerusakan jaringan. AktivitasM2 menyebabkan peningkatan respons Th2 sebagai imunomodulator, deposisi matriks dan remodeling jaringan. Peran makrofagpada infeksi M.tuberculosis akan menentukan kondisi inang. Jika makrofag dapat melakukan fungsi fagositosis M.tuberculosis akandimusnahkan dan inang tidak terinfeksi. Mycobacterium TB yang patogen dapat dengan mudah menghindari fagositosis dan berhasilmenghambat otofagi makrofag. Peningkatan otofagi akan meningkatkan efikasi BCG maupun vaksin lainnya dan dengan menggunakanpendekatan merangsang otofagi untuk membasmi TB sangat berguna sehingga pengobatan berbasis otofagi untuk TB dapat segeradiwujudkan.
CD4+ DAN CD8+ INTERFERON GAMMA TUBERKULOSIS PARU AKTIF DAN TUBERKULOSIS LATEN Betty Agustina Tambunan; John Wiwin; Jusak Nugraha; Soedarsono Soedarsono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1116

Abstract

Tuberculosis (TB) is a global health problem. Immune response through CD4+ T cells and CD8+T cells is needed to produce Interferongamma (IFN-γ). IFN-gamma is a cytokine that can kill Mycobacterium tuberculosis. Not all individuals will lead to illness or activediseases. The aim of this study was to know the cellular immune response like IFN-gamma expression of T-CD4+ cells and CD8+ cellsbetween active TB with latent TB. The design of the study was cross sectional obervational in a population suffering from active andlatent TB. The subjects consisted of 11 (eleven) active TB patients and 10 (ten) latent TB patients from the Special Pulmonary Hospitaland the Dr Soetomo Hospital, Surabaya. The examination of interferon gamma expression of CD4+ and CD8+ was by Flowcytometrymethod. These results were analyzed by Student t test or Mann-Whitney test. The mean CD4+ percentage of active TB (28.75%) waslower than the latent one (TB) (33.21%) but no significant difference (P value=0.114) was shown. The mean CD8+ percentage ofactive TB (30.46%) was higher than the latent one (TB) (28.87%) but no significant difference (P value=0.481) was found. Themean CD4+IFN-γ percentage of active TB (2.51%) was higher than latent one (TB) (1.10%) and there was a significant difference(P value=0.014). The mean CD8+IFN-γ percentage of active TB (2.91%) was lower than latent one (TB) (4.41%) and there was asignificant difference (P value=0.006). Based on this study, it can be concluded that the mean CD4+IFN-γ percentage of active TB washigher than latent TB and there was a significant difference. The mean of CD8+IFN-γ percentage of latent TB was higher than the activeone (TB). This suggested that CD8+ has a dominant part in latent TB and may be caused by the role of other cytokines, or genetics,nutrition, and body mass index factors.
CORRELATION BETWEEN IFN-ɤ LEVELS, CHEST RADIOGRAPHY AND THE POSITIVITY OF SMEAR SPUTUM IN NEW TB CASES AT THE DR.SOETOMO HOSPITAL Yessy Puspitasari; Jusak Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1316

Abstract

Tuberculosis is an infectious disease attacking lungs, triggering damage, and pulmonary dysfunction. Host cytokine responses will influence tuberculosis manifestations. The main host immune response is cellular immunity, and Delayed-Type Hypersensitivity (DTH). IFN-γ produced by Th-1, is a major cytokine acting to eliminate TB bacteria through macrophage activation. Chest radiography has an important value for the diagnosis of TB, especially in negative sputum smears. Radiological manifestations depend on several factors including host prior to TB exposure, age, and immune status. Sputum smear is also important in diagnosing, and assessing response to treatment of adult pulmonary TB. A cross-sectional study, comprising a total of 36 new pulmonary TB cases at the Dr.Soetomo Hospital who met the inclusion, and exclusion criteria, were establish. Plasma IFN-γ was examined by ELISA. Chest radiography was divided into three categories based on the National Tuberculosis Association of USA. Sputum smear data were taken from medical records. There were significant correlations between IFN-γ plasma levels with chest radiography (r= 0.365; ρ=0.029), IFN-γ with positive sputum smear (r= 0.447; ρ=0.006), positive sputum smear with chest radiography (r=0.674; ρ=0.001) IFN-γ plasma levels could reflect lesion area on chest radiography, and sputum smear positivity. IFN-γ plasma levels, chest radigraphy, and sputum positivity may reflect a Th-1immune response, so the more severe level of diseases, an immune response Th-1 become more activated. 
Co-Authors Abdul Hafid B Agung Dwi Wahyu W Agung Dwi Wahyu Widodo Agustiningrum, Indah Akhmad Setyo Rahman Alfia Andriyani Amellya Octifani aminuddin, mohammad Anak Agung Istri Sri Wiadnyani Andrianto Anita Budiarti Anna Roosdiana Annette d’Arqom, Annette Anton Yuntarso Aryati Aryati Aryati Aryati Ashon Sa'adi Ashon Sa’adi Ashon Sa’adi Audrey Gracelia Riwu Awalia Awalia Ayu Imamatun Nisa Bambang Purwanto Bastian Bastian Bastian Bastian Beatrix, Stephanie Besin, Valentinus Betty Agustina Betty Agustina Tambunan Buana, Dwi Candra Budi Prasetyo Budi Utomo Budiutari, Ni Nyoman Budy, Theresia Indah Callixte, Cyuzuzo Caroline Caroline Ch. Destri Wiwis Wijayanti Christina Destri Citra Indah Setyaningrum Cynthia Cynthia Ayu Permatasari Cynthia Ayu Permatasari Cynthia Cynthia Cynthia Cynthia Cyuzuzo Callixte Darmadi, Epriyanto T Dheasy Herawati Diyan Wahyu Kurniasari Djoko Agus Purwanto Dominicus Husada Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwiyanti Puspitasari, Dwiyanti Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim, Eddy Mart Edhi Rianto Elok Budi Retnani Elvan Dwi Widyadi Erfina Lim Erna R Tobing Erwin Astha Triyono Esthy Poespitaningtyas Febtarini Rahmawati Ferdy R. Marpaung Ferdy Royland Marpaung Foe, Kuncoro Francisca Srioetami Tanoerahardjo Francisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransiska Fransiska Fransiska Sri Oetami Gede Arie Wijaya Handayani, Luh Putu Trys Monika Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hans Kristian Nugraha, Hans Kristian Hantoro Gunawan Hari Basuki Notobroto Hari Basuki Notobroto Hari Setiono Harianto Notopuro Hartono Kahar, Hartono Hendy Wijaya Herin Mawarti Heru Setiawan Hery Priyanto Hevi Wihadmadyatami Holland Lydia Marpaung Huda Shalahudin Darusman I Made Andika Bara Kusuma Idha Kusumawati Ilham Ilham Irvan Ipandi Januarti, Catur Ifda Joewono Soeroso John Wiwin Julious Julious julious, julious Kadeq Novita Prajawanti Kasiyati, Menik Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kemas Ya’kub Rahadiyanto kurniati, nova Lisa Soegianto Lumempouw, Silvia Luminto, Dian Lutfi Dewanda Nugroho M. Aminuddin M. Aminuddin M. Irsan Saleh Mangestuti Agil Maya E. Roring Meiti Muljanti, Meiti Mohammad Aminuddin Mohammad Hasan Machfoed Muhammad Amin Muhammad Amminuddin Muhammad Hamdan Muhammad Hamdan Muhammad Irsan Saleh Muhammad Nazarudin Munawaroh Fitriah Munawaroh Fitriah Munawaroh Fitriah Myrza Elita Naesilla Naesilla Nico Jafet Ningrum, Emilna Mega Ninik Asmaningsih Soemyarso Notopuro, Paulus Budiono Nova Kurniati Nova Kurniati Nova Kurniati Noviana, Rachmitasari Nugraha, Hans K Nur Hayati Nur Lailatul Fadhilah Oetami, Fransisca Sri Oryza Chrisantia Oski Illiandri Patria Dewi Pande Pratama, Dyah Ayu Oktaviani A Prihantika S., Sabrina Pudji Lestari Purwanta, Marijam Purwoko, Agus Puspitasari, Yessy Putri, Indah Aprianti Rahadiyanto, Kemas Ya'Kub Rahajuningsih Dharma Rahajuningsih Dharma Rahayu Nofita Sari Rahma Indah Pratiwi Ravell Hansen Untono Rendy, Achmad Nur Retno Indrawati Rifa’I, Muh Husni Risky V. Prasetyo Rochmah Kurnijasanti Roedi Irawan Rusli, Musofa Ryzky Widi Atmaja S. Soedarsono S.Pd. M Kes I Ketut Sudiana . Sabri Prihantika Sabrina Prihantika Saleh, M Irsan Saputra, Angky Saputri, Agatha Efrad Senny Yesery Esar Setiawan, Lidwina Tri Kristanti Shahzad Shoukat Sheila Clarissa Shoukat, Hamad Shoukat, Shahzad SILMI MARIYA Silvia F. Lumempouw Silvia Francina Lumempouw Sinansari, Restry Siti Nur Husnul Yusmiati Siti Rahmawati Soedarsono Soedarsono Soedarsono Soedarsono Soelistijo, Soebagijo Adi SP Edijanto Srikanth Karnati Süleyman Ergün Suryawan, I Gde Rurus Susi Oktaviani Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanoerahardjo, Francisca Srioetami Tedja, I G.A. Wiradari Theresia Indah Budhy Theresia Indah Budhy Sulisetyawati Triagung Ruddy Tuyishimire Irene Utariani, Arie UUS SAEPULOH Wahyu Dewi Tamayanti, Wahyu Dewi Wahyu Setiani Wibowo Wahyu Setiani Wibowo Winthoko, Eka Nora Vitaloka Aprilia Putri Wulandari, Dian Novita Wuryanto Hadinugroho Yetti Hernaningsih Yoes Prijatna Dachlan Yohanes Adrian Kapri Negara Yuani Setiawati Yudy Tjahjono Yufita Ratnasari Wilianto Yulia Nadar Indrasari Zen Hafy