Article Per Year (5 Year)
p-Index From 2021 - 2026
2.33
P-Index
This Author published in this journals
All Journal Majalah Obat Tradisional Universa Medicina Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Medik Veteriner JPSCR : Journal of Pharmaceutical Science and Clinical Research Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Fisheries & Marine Jurnal Widya Medika
Kuncorojakti, Suryo
Program Studi Sarjana Kedokteran Hewan Fakultas Kedokteran Hewan Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Surabaya, Indonesia 60115.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 16 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 16 Documents
Search

 1   2 
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Novel Surface Marker for the Prospective Mesenchymal Stem Cell Characterization from Rabbit Visceral Adipose Tissue Kuncorojakti, Suryo; Yudaniayanti, Ira Sari; Susanti, Lina; Susilawati, Helen; Aswin, Ahmad; Diyantoro, Diyantoro; Rodprasert, Watchareewan
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.1-9

Abstract

Rabbit as a laboratory animals play an important role in bridging basic research to clinical application. The exploration of rabbit mesenchymal stem cell (MSC) is still facing an obstacle regarding the standardization of characterization of MSC. This study is aimed to explore the novel candidate of rabbit MSC surface marker as an effort to establish the goal standardization of rabbit MSC. Three 2-month-old male White New Zealand rabbits weighing 2 kg were used as visceral rabbit adipose derived mesenchymal stem cell (Rab-ADMSC) donors. The cells were characterized according to their morphology characteristic, clonogenic and three-lineage differentiation capacity. Flow cytometry was used to evaluate the MSC surface markers of Rab-ADMSC against CD81, CD29, CD34 and CD45. The results of this study revealed that the Rab-ADMSC posses characteristic of MSC according to their morphology and differentiation capacity. It can be concluded that CD81 surface marker can be proposed as a stable alternative candidate marker for rabbit MSCs. This data is supported by other phenotypic characteristics of MSCs, both in morphology and in the ability of visceral Rab-ADMSCs to differentiate into adipogenic, osteogenic, and chondrogenic lineages.
Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice Tjahjono, Yudy; Wedharga, I Gede Putu Adhi; Novita, Bernadette Dian; Tahalele, Paul; Wijaya, Hendy; Soediono, Endang Isbandiati; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.308-317

Abstract

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.
A Comparative Study Between E-Cigarette Vapor and Cigarette Smoke Exposure on the Lungs of Mice (Mus musculus) Putri, Mirtanti Kiswandi; Hestianah, Eka Pramyrtha; Kuncorojakti, Suryo; Widiyatno, Thomas Valentinus; Sukmanadi, Mohammad; Santoso, Kuncoro Puguh; Plumeriastuti, Hani; Hidayatik, Nanik; Khairullah, Aswin Rafif
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.102-108

Abstract

Background: Cigarettes are tobacco products processed with or without additives and are known to contain addictive substances that pose serious health risks to individuals and the public. Inhaled cigarette smoke directly stimulates the activation of macrophage cells in the respiratory tract. Among the affected cells, type II alveolar cells which produce pulmonary surfactant to reduce alveolar surface tension are particularly vulnerable to smoke-induced damage. Purpose:  This study aimed to compare the effects of conventional cigarette smoke and e-cigarette vapor exposure on the number of alveolar macrophages and type II alveolar cells in male mice (Mus musculus). Method: A total of 28 male mice were randomly divided into seven groups. The control group (C) was exposed to ambient air only. Groups A1, A2, and A3 were exposed to 1, 2, and 3 conventional cigarettes per day, respectively, while groups B1, B2, and B3 were exposed to 0.7 mL, 1.3 mL, and 2 mL of e-liquid vapor per day, respectively. All exposures were conducted for 36 consecutive days. Results: A significant difference (p<0.05) was observed in the number of alveolar macrophages among groups C, A1, A2 , A3 , B2 , and B3 , whereas no significant difference was found between A1 and B1. The number of type II alveolar cells significantly differed (p < 0.05) across all groups: C, A1, A2, A3, B1, B2, and B3. Conclusion: Both conventional cigarette smoke and e-cigarette vapor increase alveolar macrophage counts and reduce type II alveolar cell numbers compared to controls. Although both exposures pose health risks, e-cigarette vapor appears to cause milder pulmonary effects than conventional cigarette smoke.
Oil-Adjuvanted Polyvalent Formalin-killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.82866

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation.
PROTOCOL OPTIMIZATION OF RT-qPCR FOR NF-ΚB DETECTION IN CARDIAC TISSUE FROM HFHS-FED MICE Jaya, Ferdinand Wiliam; Novita, Bernadette Dian; Suwandito, Laurensius; Wijaya, Hendy; Tjahjono, Yudy; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
JURNAL WIDYA MEDIKA Vol. 12 No. 1 (2026): March
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v12i1.8196

Abstract

Accurate detection of gene expression in challenging tissues requires methodological optimization to ensure reproducibility. Cardiac tissue, with its low cell density and variable RNA yield, presents particular difficulties for RT‑qPCR analysis. This study aimed to refine NF‑κB detection in HFHS‑fed C57BL/6 mice by comparing a standard 2‑ΔΔCt approach with an optimized protocol incorporating individualized calibrators adjusted for RNA concentration. Six male mice were divided into standard-diet (STD) and high‑fat, high‑sucrose (HFHS) groups, and cardiac RNA was extracted after 8 weeks of feeding. While relative NF‑κB expression trends were higher in the HFHS group, differences were not statistically significant; however, the optimized method consistently reduced variability and improved measurement reliability compared to the generalized approach. These findings highlight the value of individualized calibration as a protocol refinement for RT‑qPCR, offering a more rational framework for gene expression analysis in heterogeneous or technically demanding tissue samples.
Co-Authors A'la, Rofiqul Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Annas Salleh Annise Proboningrat Arimbi Aswin, Ahmad Bambang Sektiari Lukiswanto Boedi Setiawan Chozin, Aminullah Alhady Chusniati, Sri Daruti Dinda Nindarwi Delaiah, Diena Dewi, Nina Nurmalia Dita Wisudyawati Diyantoro Diyantoro, Diyantoro Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Fecky Fernando Fredericktho Fedik Abdul Rantam Gazali Salim Hani Plumeriastuti Helen Susilowati, Helen Hendrata, Adi Pramono Heny Kristi Meitasari Hidayatik, Nanik Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Jaya, Ferdinand Wiliam Jessica Giovani Jong, FX Himawan Haryanto Khairullah, Aswin Rafif Legowo, Djoko LINA SUSANTI Lucia Hendriati Lucia Hendriati Lucia Tri Suwanti, Lucia Tri Malonda, Franklin Vincentius Martha Ervina Maulana, Firdausy Kurnia Mirny Lamid Mohammad Noor Amal Azmai Muchammad Yunus Ni Putu Pirna Wijayanti Novita, Bernadette Dian Nusdianto Triakoso Paul L Tahalele Prastiya, Ragil Angga Putra, Brilliant Dwi Putri Desi Wulan Sari Putri, Mirtanti Kiswandi Rahmahani, Jola Rao, Sherbrina Bai Seenivasa Rendragraha, Briantono Willy Rodprasert, Watchareewan Rozi Santanumurti, Muhammad Browijoyo Santoso, Kuncoro Puguh Setiawan Koesdarto Sharveihn Sathindren Sianty Dewi Soediono, Endang Isbandiati Sukmanadi, Mohammad Sumi WIjaya, Sumi Susilawati, Helen Suwandito, Laurensius Suwarno Suwarno Suzanita Utama Teguh Widodo Teguh Widodo Teguh Widodo Teguh Widodo Tita Damayanti Lestari Tjahjono, Yudy Wedharga, I Gede Putu Adhi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wijaya, Andi Yasmin Wijaya, Hendy Wilis Prasasti Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Yeni Dhamayanti Yulianna Puspitasari Yunita, Maya Nurwartanti