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Journal : Narra J

Correlation between circulating fibrosis biomarkers with left atrial function and left atrial volume index in rheumatic mitral stenosis Asrial, An A.; Reviono, Reviono; Soetrisno, Soetrisno; Setianto, Budi Y.; Widyaningsih, Vitri; Nurwati, Ida; Wasita, Brian; Pudjiastuti, Anggit
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.293

Abstract

Mitral stenosis is the most common rheumatic heart disease (RHD) disorder worldwide, including in Indonesia. This pathological condition causes left atrial pressure, leading to left atrial fibrosis that affects the structure and function of the left atrial as well as the clinical condition. The aim of this study was to assess the correlation between circulating fibrosis biomarkers with net atrioventricular compliance (Cn) as a parameter of left atrial function, and left atrial volume index (LAVI) as a parameter left atrium structure of changes. A cross-sectional study was conducted at Panti Rahayu Hospital and Permata Bunda Hospital, Purwodadi, Central Java, with a total of 40 RHD patients with severe mitral stenosis. The ELISA was used to measure the levels of carboxy-terminal propeptide of type I procollagen (PICP), matrix metalloproteinase I (MMP-1), tissue inhibitor matrix metalloproteinase 1 (TIMP-1), and transforming growth factor-β1 (TGF-β1). The left atrial function was assessed by measuring Cn, and the LAVI parameters were measured to assess left atrium structure/size. The mean levels of circulating fibrosis biomarkers were as follows: PICP 153.96±89.12 ng/mL; MMP-1 1.44±2.12 ng/mL; MMP-1/TIMP-1 ratio 0.38±0.54 and TGF-β1 2.66±1.96 pg/mL. From the echocardiographic evaluation, the mean Cn was 5.24±1.93 mL/mmHg and the mean LAVI was 152.55±79.36 mL/m2. There were significant correlation between MMP-1 and MMP-1/TIMP-1 ratio with Cn (r=0.345 and r=0.333, respectively; both had p<0.05). PICP and TGF-β1 biomarkers did not significantly correlate with Cn (p>0.05). Meanwhile, none of the biomarkers had a significant correlation with LAVI (p>0.05). This study highlights that MMP-1 and MMP-1/TIMP-1 ratio are potentially to be used as markers to determine the Cn in RHD patients with severe mitral stenosis. However, further studies with a higher sample size are needed to confirm this finding.
Evaluating autologous peritoneum grafting for enhanced healing of bile duct injuries: A preliminary data from an animal study Nugroho, Anung N.; Mudigdo, Ambar; Soetrisno, Soetrisno; Yarso, Kristanto Y.; Nurwati, Ida; Indarto, Dono; Pamungkasari, Eti P.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1873

Abstract

Increased incidence of laparoscopic cholecystectomy-related bile duct injuries (BDIs), combined with its risk of serious complications and mortality, highlights the need for a more effective repair technique. Although the use of autologous graft in BDI repair has been promoted, the role of autologous parietal peritoneum remains underexplored. The aim of this study was to evaluate the effect of autologous parietal peritoneum grafts in rabbit models of partial BDI, emphasizing its effect on the expression of cluster of differentiation 68 (CD68) and transforming growth factor-β (TGF-β). An experimental post-test-only design was employed, using 27 male New Zealand rabbits (Oryctolagus cuniculus) aged 8–10 months. The rabbits were allocated into three groups: control (primary closure), autologous parietal peritoneum graft, and autologous gallbladder graft. Partial BDI measuring 15×5 mm were surgically created and repaired according to group assignments. The expression of CD68 and TGF-β were measured via enzyme-linked immunosorbent assay (ELISA), while the anastomosis was pathologically examined through hematoxylin and eosin (H&E) staining on days 3, 7, and 14 post-surgery. Statistical analysis was performed using analysis of variance (ANOVA) followed by Bonferroni post hoc tests. No statistically significant difference was observed in the expression of CD68 or TGF-β among the three treatment groups on days 3, 7, and 14 post-surgery, indicating that the effects of autologous parietal peritoneum graft were comparable to the control and the autologous gallbladder graft in promoting wound healing. Fibroblast density on day 3 was significantly lower in the parietal peritoneum group (p=0.040), reflecting delayed recruitment, but normalized by day 14, indicating successful integration and remodeling. The study highlights the potential role of autologous parietal peritoneum grafts for BDI.
Co-Authors A. Guntur Hermawan Adi Prayitno Ambar Mudigdo Anik Lestari Asrial, An A. Baihaqy, Fathu Thaariq Bambang Purwanto Betty Saptiwi Betty Saptiwi Betty Saptiwi Brian Wasita Christanto, Antonius Danus Hermawan Danus Hermawan Dicky Maulana Lazuardi Diding H P Diding Heri Prasetyo Diding Heri Prasetyo Dono Indarto Dyah Rahmawatie Ratna Budi Utami Dyah Ratna Budiani Dyah Ratna Budiani Dyah Ratna Budiani Eriana Melinawati Eric Edwin Yuliantara Eti Poncorini Pamungkasari Fadhilah, Azzahra Nur Fahmi, Muhammad Izzul Widad Gresmita Rindi Winarti Hadi, Cahyono Harioputro, Dhani Redhono Hermawan, Danus Hermawan, Danus Ika Tristanti Indri Erwhani Jarot Subandono Jarot Subandono Jarot Subandono Khoirunnisa, Rahmi Lazuardi, Dicky Maulana Mahendra, Fadia Yuda Martini Martini Martini Martini Martini Martini Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Mutmainah Mutmainah Nabila Aushaf Prasetyo Nafisa, Putri Amalina Nanang Wiyono Noni Kartika Sari Nugroho, Anung N. Nur Hafidha Hikmayani Nur Hafidha Hikmayani Nutria Widya Purna Anggraini Octavia, Dhamayanti Eka Pamungkasari, Eti P. Paramasari Dirgahayu Prihandjojo Andri Putranto Pudjiastuti, Anggit Putranto, R. Prihandjojo Andri Putranto, Wachid Rachmi Fauziah Rahayu Rahardjo, Setyo Sri Ratnasari Ratnasari Reviono Reviono Risya Cilmiaty A. R. Risya Cilmiaty AR Risya Cilmiaty, Risya Rosyida, Rufaida Rusliana, Eka Sari, Noni Kartika Sarsanti, Arifa Sherina Noor Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Sarsono Selfi Handayani Selfi Handayani Selfi Handayani Selfi Handayani Setiamika, Made Setianto, Budi Y. Sigit Setyawan Sineri, Dessy Ikasartika Siti Aisyah Siti Marufah Soetrisno Soetrisno Soewondo, Widiastuti Sri Hartati Hadinoto Sri Haryati Sri Sulistyowati Sumardiyono Sumardiyono Sumardiyono, S Tonang Dwi Ardyanto Uki Retno Budihastuti Vitri Widyaningsih Wahyu Anjas Sari Wesliaprilius, Todung Antony Wiyono, Nanang Wuriani, Wuriani Yarso, Kristanto Y. yoseph Indrayanto Yudhani, Ratih Dewi Yulia Sari Yulyanti, Yulyanti