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The first evaluation of glucose-6-phospate dehydrogenase defciency (G6PD) gene mutation in malaria endemic region at South Central Timor (SCT) district, Eastern Indonesia 2014–2015 Jontari Hutagalung; Hari Kusnanto; S. Supargiyono; P. Purwono; Sadewa Ahmad Hamim; Darojatun Ida; Satyagraha Ari Winasti; Novijanti Rintis; Triwibowo A. Garjito; Mega Tyas Prihatin; Bai Apris; Bansai Immanuel; Kik Hao Samuel; Hananta Linawati
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1115.764 KB) | DOI: 10.22146/ijbiotech.24194

Abstract

Primaquine (PQ) is a key drug in the malaria pre-elimination stage. However, PQ can trigger acutehemolysis for people with G6PD defciency (G6PDd). In 2013, 15–25 million Indonesian people were infected with malaria, with 30,000–38,000 deaths each year mostly in eastern Indonesia with API= 15.6 %. Recently, the Ministry of Health of the Republic of Indonesia announced a plan to reach the pre-elimination stage based on WHO guidelines. This study assesses whether eastern Indonesia should proceed with the activities of malaria pre-elimination. A total 555 healthy people in fve subdistricts in eastern Indonesia were selected by systematic random samping. All data were collected using a standard questionnaire, physical examination, and laboratory tests. PCR and DNA sequencing protocols followed respective manufacture’s instructions. Statistical analysis by bivariate with α= 0.05 and 95% CI were performed using the SPSS software package. Based on the nested PCR, the result showed a malaria prevalence of 32.6% with being the dominant species (52.5%). Malaria cases were found in all study sites and not using a bed net was the moost signifcant risk factors with Exp B= 1.54 with 95% CI= 0.99–2.38. G6PDd prevalence was 16.6%, the highest G6PDd ever found in Indonesia with variant molecular dominant 10.883 T>C and one sample with a heterozygous female. Malaria pre-elimination in eastern Indonesia should be delayed. High risk patients should be tested for enzyme G6PD activities before antimalarial administration.
Distal Renal Tubular Acidosis (dRTA) Among Southeast Asian Ovalocytosis (SAO) Patients in Malaria Endemic Area of Sekotong, Lombok Island I Gusti Ayu Nyoman Danuyanti; Tasmini -; Ahmad Hamim Sadewa
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4568

Abstract

Introduction: Southeast Asian Ovalocytosis (SAO) is caused by 27 bp deletion of the band 3 protein gene in erythrocyte membrane and characterized by oval erythrocyte. The erythroid band 3 (AE1) gene isexpressed not only in erythrocyte membranes but also in the cell membrane of α-collecting renal tubular functions in the secretion of acid in renal tubules and HCO3 -/Cl- anion exchange. An alteration of the band 3 (AE1) gene functions in cell of α-collecting renal tubules reduces HCO3-/Cl- ion exchange resulting in decreased secretion of H+ ions and disturbances in the process of pickling urine as an indicator of distal renal tubular acidosis (dRTA).Objectives: To determine the occurrence of dRTA among Southeast Asian Ovalocytosis (SAO) patients in malaria endemic area of Sekotong, Lombok Island by analyzing expression of erythroid band 3 (AE1) gene.Methods: Ovalocytosis degree determined by oval erythrocytes morphology of 20%-100% in blood film. The 27 bp deletion of band 3 (AE1) gene was analyzed by polymerase chain reaction (PCR). An indicators of the dRTA was determined the pH of urine, levels of bicarbonate (HCO3-), potassium (K+) and chloride (Cl-) in the blood.Results: The degree of ovalocytosis more than 50% was found in 35,7% individuals and below 50% was found in 64,3% individuals. Percentage of 27 bp deletion of band 3 (AE1) gene among subject of ovalocytosispatients was 35,7% (n = 28) and frequency of the dRTA among SAO patients was 20% (n = 10). Individuals with SAO do not generally have dRTA. The presence of the two conditions in the same individuals suggests that there may be a common underlying molecular defect or genetic basis for SAO and dRTA.Conclusion: There is a relationship between the 27 bp deletion protein band 3 (AE1) gene with distal renal tubular acidosis (dRTA) among Southeast Asian Ovalocytosis (SAO) patients in Sekotong, Lombok Island, even the percentage is low.Keywords: distal renal tubular acidosis, Southeast Asian Ovalocytosis, Sekotong, (Lombok Island)
CD4 COUNT FROM CRYOPRESERVATION OF BUFFY COAT AND PBMC Rasmaya Niruri; Inna Narayani; Wayan Tunas Artama; Mantik Astawa; Ahmad Hamim Sadewa
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and PeripheralBlood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for eachtube) were drawn from one adult healthy subject. The samples were categorized into five group beforemeasuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh <GII>and frozen <G-III>] , and PBMC resulted from BC and ficoll isolation [fresh <G-IV> andfrozen <G-V>]. Each group was replicated three times. Blood storage before preparation was less thanfour hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI)was conducted. The mean value of CD4 count (cell /mu1) were 522 (G-I), 1410 (G-II), 906 (G-III), 807(G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G-III) was higher (906 cell /mu1) than PBMC (G-IV) sample (733 cell /mu1).
Polymorphism of vascular endhothelial growth factor (VEGF) gene insertion/ deletion -2549 as risk factor of diabetic retinophathy in Javanese patients with type 2 diabetes Jems Kifen Roget Maay Ahmad Hamim Sadewa Maliyah Madiyan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 02 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Diabetic retinopathy (DR) is a visual disorder caused by the diabetic microvascular complications.Genetic polymorphism in the vascular endothelial growth factor (VEGF) gene plays an importanrole in the susceptibility of DR. The aim of this study was to evaluate the association of thepolymorphism of VEGF gene insetion/deletion (I/D) -2549 with DR in Javanese type 2 diabetesmellitus (DM) patients. This was a case control study involving 40 Javanese type 2 DM patientswith DR as case subjects and 40 Javanese type 2 DM patients without DR as control subjects.Type 2 DM patients with DR were recruited from Eye Polyclinic, whereas type 2 DM patientswithout DR were recruited from Endocrine Polyclinic of Dr. Sardjito General Hospital, Yogyakarta.Genotyping of VEGF gene I/D-2549 was conducted using PCR-RFLP method. Plasma VEGFlevels were measured using Enzym-Linked Immunosorbent Assay (ELISA). The genotype distributionof DD (67.5%) and the allele frequency of D (82.5%) in type 2 DM patients with DR was higherthan those without DR (27.5% for DD genotype and 56.3% for D allele). The OR of DD and IDgenotypes versus II genotype between type 2 DM patients with DR and without DR was 6.882(95%CI: 0.789-60.060; p=0.048), whereas OR for the D allele versus I allele between type 2 DMpatients with DR and without DR was 3.667 (95%CI: 1.773-3.667; p=0.000). The plasma VEGFlevels of DD genotype (92.16±49.73 pg/mL) were significantly higher than ID genotype(42.70±33.29 pg/mL) in type 2 DM patients (p=0.000). In conclusion, the polymorphism ofVEGF gene I/D -2549 is associated with DR in Javanese type 2 DM patients. The DD genotypeand D allele of the VEGF gene polymorphism are the risk factor of DR in those patients. Theassociation of the polymorphism of VEGF gene with DR may be explained with the high plasmaVEGF level.Keywords: vascular endothelial growth factor (VEGF) - polymorphism - diabetes - retinopathy- Javanese patients
The relationship between vitamin A and ferritin towards malondialdehyde level among Javanese male smokers Ajeng Viska Icanervilia; . Sunarti; Ahmad Hamim Sadewa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Cigarette smokes produce a large number of oxidants and promote secretion of ferritin byalveolar macrophages which are potential to encourage the lipid peroxidation. Malondialdehyde(MDA) is used as a parameter of lipid peroxidation. The study was aimed to evaluate therelationship between blood level of vitamin A and ferritin and MDA among Javanese malesmokers. Sixty men who lived in Purworejo District, Central Java, Indonesia comprising 30smokers as case group and 30 nonsmokers as control group were involved in this study. Bloodsample was obtained from cubiti vein and then centrifuged to obtain plasma or serum. Blood levelsof vitamin A, ferritin and MDA were measured by HPLC, ELISA and spectrophotometric methods,respectively. The result showed that the blood vitamin A, ferritin, and MDA levels in smokers were25.09±9.51μg/dL, 35.50±24.17ng/dL, 1.15±0.42μg/L, respectively, whereas in non smokers, theywere 26.11±9.19 μg/dL, 38.60±15.25 ng/dL, 1.06±0.50 μg/L, respectively. Therewas no significantdifference of the blood vitamin A, ferritin, and MDA levels between smokers and the non smokers(p>0.05). The linear regression analysis indicated that there was negative relationship betweenblood vitamin A and MDA levels although it was not significant (p=0.052), while blood ferritin andMDA levels had a significantly positive relationship (p=0.010). In conclusion, the low level of bloodvitamin A among cigarette smokers does not lead to high blood MDA level, while high level of bloodferritin among smokers leads to high blood MDA level. 
Relation between -carotene and ferritin upon malondialdehyde in Javanese male smoker Naomi Yoshuantari Ahmad Hamim Sadewa Sunarti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 01 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Smoking of cigarette can cause additional free radicals. Oxidative damage is resulted from the accumulation of freeradicals in the body. Malondialdehyde (MDA) is the end product of free radicals, a marker of oxidative damage. -carotene is pro-vitamin A, an antioxidant known to quench singlet oxygen. Ferritin is thought to release excessiveiron in smokers, thereby increasing the oxidative stress. The aim of the study is to evaluate the relation between -carotene and ferritin toward MDA level in Javanese smokers. This study was carried out in a case-control, crosssectional design nested with cluster sampling. Participants were Javanese smokers and non-smokers in Purworejodistrict, Central Java. Samples and data were obtained secondarily. The results were analyzed using independentsamples t-test and linear regression. The results showed that there was very weak negative correlation between -carotene with MDA (R square=0.013; p value=0.320) and very weak positive correlation between ferritin withMDA (R square=0.043; p value=0.067). There was no statistically significant relation of -carotene with MDA.Ferritin level was marginally influential upon MDA level as the marker of lipid peroxidation between smokers andnon-smokers. Aging apparently became the confounding variable by influencing the lipid peroxidation more efficientlythan smoking itself (p = 0.013). In conclusion, there was no significant relation between -carotene and ferritinwith MDA in smokers.Key words : smoking – cigarette – β-carotene – ferritin – malondialdehyde
Aldose reductase genetic polymorphism is a risk factor of diabetics retinopathy among type 2 diabetes mellitus in Yogyakarta, Indonesia Jujuk Anton Cahyono Ahmad Hamim Sadewa Tasmini
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Diabetes mellitus (DM) is a metabolic syndrome characterized by hyperglycemia and glucose intolerance, due toinsulin resistance, insulin deficiency, or both. Diabetics retinopathy (DR) is a DM complication due to retinal abnormality,that causes vision reduction and even blindness. The association between DR and aldose reductase C-106T (ALR C-106T) gene polymorphism has been reported in previous studies. This genetic polymorphism increases the sorbitollevel inside erythrocyte and pericyte in the retinal membrane that leads to weakness of retinal capillary vessel andmicroaneurism. The aim of this study was to know the presence of ALR C-106T gene polymorphism and its frequencydistribution among diabetics Javanese patients in Dr. Sardjito General Hospital Yogyakarta, Indonesia. In addition,this study also aimed to analyze the difference of erythrocytes osmotic fragility (EOF) among ALR genotypes in type2 diabetics patients with DR and without DR and to analyze whether ALR genetic polymorphism is a risk factor ofDR in type 2 diabetic patients. This was a case control study that involved 40 diabetics patients with DR as case and40 diabetics patients without DR as control groups. The C-106T ALR gene polymorphism was determined bypolymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) method. Erythrocytes osmoticfragility was analyzed using spectrophotometer. Genotype and allele distributions were analyzed using x2 and otherdata were analyzed using independent t-test and Mann-Whitney, with p<0.05 was considered as significantlydifferent. The results showed that in type 2 diabetics patients with DR, 33 patients (82.5%) were CC homozygoteindividuals and 7 patients (17.5%) were CT heterozygote individuals. In type 2 diabetics patients without DR, 27patients (67.5%) were CC homozygote individuals and 13 patients (32.5 %) were CT heterozygote individuals. Thegenotype and allele distributions were not significantly different between two groups (p=0.121 for genotype,p=0.151 for allele). Odds Ratio of genotype was 2.270 while allele was 2.023. Erythrocytes osmotic fragility of CCgenotype was higher than CT genotype (p=0.047). In conclusion, there was no significant difference between CCand CT genotype distribution among type 2 diabetics patients with and without DR. Erythrocyte osmotic fragility ofCC genotype was higher than CT genotype. C-106T gene polymorphism was a risk factor of DR in type 2 diabeticpatients.Key words : ALR genes – polymorphism - type 2 DM - diabetic retinopathy - erythrocytes osmotic fragility
Assessment of the application level of radiation protection and awareness of radiation safety regulations among the radiographers at Yogyakarta Special Region, Indonesia Ahmad Rafiq Mohammad Abu Arrah Arif Faisal Ahmad Hamim Sadewa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

A strong relation between cancers and radiation exposure has been reported. Radiation may damage DNA in thecell. Therefore, radiation protection program must be applied in the radiology department. Morover, the radiographershould have high level of awareness and risk assessment for radiation. Personal radiation monitoring is one of themain radiation protection, especially for pregnant worker and her fetus. This study was conducted to evaluate theapplication, awareness and risk assessment levels of radiation protection among radiographers at hospitals inYogyakarta Special Region, Indonesia. This was a descriptive study, applying a cross sectional survey at hospitals inYogyakarta. The subjects were radiographers of both governmental and private hospitals. There were 101 respondentsfrom a total of 124 radiographers. The data obtained were tabulated and analyzed using Chi Square test. The studyrevealed that 69.3% of the respondents had low application level of radiation protection, 19.8% did not know themeaning of ALARA (As Low As Reasonably Achievable), 50.5% were not aware of Inverse Square Law. The studyalso reported that 36.6% of the respondents did not know the amount of radiation that entered their body last year,61.4% of radiographers thought that the risk assessment of radiation was not enough, 18.8% of radiographerswere never use any radiation monitoring device, and 90.1% stated that there was no additional protection orradiation monitoring to the pregnant radiographer. However, there were no significant differences between durationof working, type of imaging modality, academic level, and training course for radiation protection. In conclusion,there was no difference in the application, awareness, and risk assessment levels of radiation protection among theradiographers at hospital in Yogyakarta Special Region between duration of working, type of medical imaging modality,academic level, and training on radiation protection. In addition, the application, awareness, and risk assessmentlevels of radiation protection were not sufficient.Key words: radiation protection – ALARA – radiographer – hospital – risk assessment
Clinical Characteristics of Adult Uncorrected Secundum Atrial Septal Defect, A Pilot Study Lucia Krisdinarti; Anggoro Budi Hartopo; Dyah Wulan Anggrahini; Ahmad Hamim Sadewa; Abdus Samik Wahab; Budi Yuli Setianto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 2 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.98 KB) | DOI: 10.19106/JMedSci004802201603

Abstract

ABSTRACTAtrial septal defect (ASD) is the most frequent congenital heart disease in adulthood. Pulmonary hypertension (PH) complicating ASD compels patients seeking medical assistance because of its disabling symptom. Most adult ASD develop PH which render significant morbidity and mortality. The aim of the study is to characterize the clinical profiles of adult patients with ASD. The study design was cross sectional. The subjects were enrolled consecutively from outpatient clinics and inpatient wards. The demography, medical and imaging data were collected and recorded in case report form. Descriptive statistics was applied to characterize the subjects. Seventy-six subjects were enrolled. The majority were women (77.6 %) in the productive and child-bearing ages (63.2%). The most common symptoms were dyspneu on effort, fatigue, and palpitation. Most subjects had functional capacity of WHO class functional II (70.2 %). The mean oxygen saturation was 96.4 %. Based on the echocardiography examination, 77.6% of subjects had suffered from PH. The mean longest diameter of defects were 2.7 cm. The direction of blood flow was mostly left to right (77.6 %). Left and right ventricle function were within normal limit. Right heart catheterization showed mean left atrial pressure 11.5 mmHg, which confirmed the precapillary or arterial PH. Mean pulmonary artery pressure was 42.0 mmHg. The pulmonary artery resistance index mostly less than 4 Wood Unit/ m2 (63.7 %), indicating the feasibility to close the defect. Whereas 24.6 % of subjects were contraindicated for closing. Pulmonary artery hypertension (PAH) was diagnosed in 77.6 % subjects, meanwhile 13.2 % had borderline PAH. In conclusion, most adult ASD patients had developed PAH, mostly young women in productive ages, mainly visited hospital due to symptom of PH, the direction of flow predominantly left to right side and mostly had reduced functional capacity.
Correlation of methylation of toll-like receptor 4 (TLR4) and interleukin-6 (IL6) promoter with insulin resistance in obese adolescents Ratih Feraritra Danu Atmaja; Dian Caturini Sulistyonigrum; Emy Huriyati; Ahmad Hamim Sadewa; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 1 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1035.405 KB) | DOI: 10.19106/JMedSci004801201602

Abstract

Chronic inflammation can lead to insulin resistance in obesity. Increased mRNA expressionof inflammatory markers such as toll-like receptor 4 (TLR4) and interleukin 6 (IL6) werereported both in the tissue and the peripheral blood of obese adolescent and correlatedwith insulin resistance. DNA methylation surrounding TSS region is known to regulate thelevel of a gene expression. The aim of the study was to investigate the methylation statusof TLR4 and IL6 promoter in peripheral blood of obese adolescent and its correlation toinsulin resistance. This was a cross sectional study with observational analytic approached.Fifty adolescents with obesity from Yogyakarta Special Region, aged 15-18 years,z-score value >2 SD, no experience of any acute infections within 2 weeks and signed ininformed consent, were selected in this study. Subjects with diabetic mellitus, incompletedata and DNA concentration of <20 μg/mL were excluded. DNA samples were extractedfrom peripheral blood of the subjects. The bisulfite converted DNA was analyzed formethylation level with direct sequencing. Correlation analysis using Spearman test wasperformed with significance value was set at p<0.05. No methylation in TLR4 promoterwas detected in all subjects. The methylation level of IL6 was transformed to categoricalvariable. Four (8%) subjects with insulin resistance and 2 (4%) without insulin resistancehad methylation on a CpG site. Methylation of IL6 was not statisticaly different betweeninsulin resistance and non-resistance adolescent (p=0.635). The significant correlationwas not found between methylation level in IL6 promoter with insulin resistance (HOMAIR)(r=0.051). Cell specific of methylation mechanism, characteristic of subjects andtypes of samples were suspected causing those results. In conclusion, no correlationbetween methylation levels of TLR4 promoter and IL6 with insulin resistance in obeseadolescent was observed in this study.
Co-Authors . Harapan . HERNAYANTI . Syahrul Abdul Salam M Sofro, Abdul Salam Abdul Salam M. Sofro Abdul Salam Mudzakir Sofro Abdul Salam Mudzakir Sofro, Abdul Salam Mudzakir Abdurahman Laqif Abdurahman Laqif Abdus Samik Wahab Abrory Agus Cahya Pramana Ahmad Husain Asdie Ahmad Husain Asdie Ahmad Husain Asdie Ajeng Viska Icanervilia Akhmad Kharis Nugroho Alfasunu, Serafim Anggelia Puspasari Anggoro Budi Hartopo Ani Kristiyani Arif Faisal Arif Faisal Arta Farmawati Arta Farmawati Atmaja, Sarah Awal Prasetyo Bai Apris Bambang Hariono Bambang Hariono Bambang Hariono Bansai Immanuel Bernadia Branitamahisi Bernadia Branitamahisi Budi Yuli Setianto Cahyono Hadi Cahyono Hadi Christine Patramurti Citra Maharani Danarsih, Dwi Eni Darojatun Ida Demas Bayu Handika Dessy Rakhmawati Emril Dewajani Purnomosari Dian Caturini Sulistyonigrum Dian Lestari, Nova Dianandha Septiana Rubi Dibyo Pramono Didik Setyo Heriyanto Djaswadi Dasuki Djaswadi Dasuki Djaswadi Dasuki Dono Indarto Dono Indarto DONO INDARTO Dwi Aris Agung Nugrahaningsih Dyah Wulan Anggrahini Ellsya Angeline Rawar Emilia Vivi Arsita Emy Huriyati Endang Mutiawati Rahayuningsih* Faisal Rahman Fransisca Shinta Maharini Hanafi, Arif Riswahyudi Hari Kusnanto Hasan Sjahrir Hemi Sinorita Hendi Wicaksono Hernayanti , Hernayanti HERNAYANTI HERNAYANTI Hernayanti Hernayanti Hexa Apriliana Hidayah I Gusti Ayu Nyoman Danuyanti I NYOMAN MANTIK ASTAWA Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ika Rahayu Ika Setyawati Ikhsan, M. Robikhul Imelda, Priscillia Indwiani Astuti Indwiani Astuti Indwiani Astuti Inna Narayani Iskandar Zakaria Jayusman, Achmad Mulawarman Jenny Hidayat Jontari Hutagalung Kik Hao Samuel Kris Herawan Timotius Kusumadewi Eka Damayanti Kusumadewi Eka Damayanti Kusumadewi Eka Damayanti Lina Choridah Linawati Hananta, Linawati Lucia Krisdinarti Lukman Hakim M. Robikhul Ikhsan Maliyah Madiyan Maria Dara Novi Handayani, Maria Dara Novi Marsetyawan HNE Soesatyo Mega Tyas Prihatin Mohammad Robikhul Ikhsan Mus, Rosdiana Mustofa Mustofa Mustofa Mustofa Ngadikun - Ngadikun Ngadikun Nor Istiqomah Nor Istiqomah Nor Istiqomah Nor Sri Inayati Nor Sri Inayati Novijanti Rintis Nurtjahjo Dwi Sasongko P. Purwono Pinda Hutajulu Pinda Hutajulu, Pinda Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti, Pramudji Prasetyastuti Prasetyastuti Prasetyastuti Prasetyastuti Prasetyastuti Prasetyastuti, . Rasmaya Niruri Ratih Feraritra Danu Atmaja Rina Pratiwi Pudja I. A Rina Susilowati RIZKI FAJAR UTAMI Rochadi Rochadi Rochadi Rochadi, Rochadi S. Supargiyono Sabirin, Rahmaningsih Mara Sarah Safira Umarghanies Saryono Saryono Satyagraha Ari Winasti Seto Priyambodo Setyo Purwono Siti Boedina Kresno Siti Wahyuningsih Sofia Mubarika Haryana Sri Sutarni Sri Wahyuni Subagus Wahyuono Subagus Wahyuono Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sunarti Sunarti Sunarti Sunarti Sunarti Sunarti Sunarti Sunarti, Sunarti Sunarto Ang Tasmini - Tasmini Tasmini Teguh Aryandono Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Totok Utoro Totok Utoro Triwibowo A. Garjito Umarghanies, Sarah Safira Viren Ramadhan Vitarani Dwi Ananda Ningrum Vitria Sari Dewi Vitria Sari Dewi Waode Astria Sahrani Wasilah Rochmah wayan T Artama wayan T Artama Wayan Tunas Artama Widhiastuti, Stefani Santi Wiryatun Lestariana Wiryatun Lestariana, Wiryatun Wiwik Handayani Yuliana Heri Soesilo Yuliana Heri Soesilo Yuliana Heri Soesilo Yuliani, Fara Silvia Yunilistiaingsih Yunilistiaingsih Yunilistiaingsih, Yunilistiaingsih Yuningtyaswari Yuningtyaswari Zainal Arifin Nang Agus Zullies Ikawati