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KEMAMPUAN MALARISIDAL NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI TERAPI AJUVAN PADA HISTOPATOLOGI HATI MENCIT (Mus musculus) PENDERITA MALARIA Pujiyanto, Mahendra; Maslachah, Lilik; Triakoso, Nusdianto; Lazuardi, Mochamad; Anwar, Chairul; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21094

Abstract

The aimed of this study is to know malaricidal ability of nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy on liver histopathological of mice (Mus musculus) which infected by Plasmodium berghei. This research uses 30 mice which are divided into six treatment groups namely K0 (not infected without treated), K+ (infected without treated), K1 (infected and treated with chloroquin), K2 (infected and treated with juwet leaf extract), P1 (infected and treated with juwet leaf extract nanoparticle) and P2 (infected and combination treated with chloroquine and juwet leaf extract nanoparticle) which infected by 1x105 in 0.2 ml of Plasmodium berghei. The data were analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. This research result showed there was significant difference (p < 0.05) between K1 and P2, but did not showed significant difference (p > 0.05) with K+, K2 and P1. Based on this research, it could be concluded that nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy estimated can increase malaricidal ability in degeneration, necrosis, portal inflammation, Kupffer cell hyperplasia, sinusoidal congestion, and hemosiderosis in mice liver which infected by Plasmodium berghei.
The Prevalence of Domesticated Geriatri Cats in Surabaya City at April-May 2019 Rahmawati, Rr Indah Nur; Triakoso, Nusdianto; Ma'ruf, Anwar
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28680

Abstract

This study is beneficial to give information about population of domesticated geriatric cats in Surabaya period from April to May 2019. This study is also beneficial to give information about likely risks of domesticated geriatric cats in Surabaya City based on gender, cat breed, and cat's disease. The design of this study was Observational Retrospective using cross-sectional method. Sampling used stratified random sampling method. This study collected primary data and was analyzed descriptively using prevalence test and relative risks. That domesticated geriatric cats in Surabaya City period from April-May 2019, the oldest cats were written 17 years for male cats and 9 years for female cats. The prevalence of domesticated geriatric cats in Surabaya City period from April-May 2019 was 9 (7,5%). Geriatric cats distributed by the gender were 5 (4,2%) male and 4 (3,3%) female, where geriatric cats distributed by the cat breed were 5 (4,2%) pure breed, 4 (3,3%) mixed breed, and 0 (0%) domestic sample. The result of geriatric cats analysis based on gender showed that male cats had a change of 1,4 times higher compared to the female to reach geriatric, whereas based on cat breed it showed pure breed had a change 3,3 times compared to mixed breed to reach geriatric. On this study, the disease often appeared in domesticated geriatric cats in Surabaya City period from April -May 2019 were: on skin, respiration, digestion, and eyes. Based on relative risk analysis on geriatric cats, it had a change of gastrointetinal tract that was higher compared to other diseases.
Screening of Blood Glucose Concentration in Domestic Cat (Felis Catus) Based on Body Condition Score, Breed, and Sex Using Portable Blood Glucose Meter Billa, Lutfiah Annisa; Triakoso, Nusdianto; Rachmawati, Kadek; Yuniarti, Wiwik Misaco; Aksono, Eduardus Bimo; Yudaniayanti, Ira Sari
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42246

Abstract

Type 2 diabetes mellitus (T2DM) is a common endocrine disease in domestic cats. Breed, sex, being overweight to an extent of obesity are predisposing factors for developing T2DM. One of the most common laboratory analyses conducted to confirm this disease is blood glucose concentrations. The aim of this research is to determine blood glucose concentration in domestic cats based on BCS, breed, and sex using a portable blood glucose meter. Research samples were obtained by taking blood samples from the marginal ear vein of 131 domestic cats that matched the sample's criteria, took at 2-hours post prandial, and drip onto the Sinocare strip. The results showed that the average blood glucose concentration was in the normal range, with a total of ideal (BCS ≥3/5) domestic cats (n=43) was 75.74 mg/dL, overweight (BCS ≥4/5) domestic cats (n=44) was 78.23 mg/dL, obese (BCS ≥5/5) domestic cats (n=44) was 89.66 mg/dL, male (n=51) was 80.57 mg/dL, female (n=80) was 81.69 mg/dL, Mixed Breed (n= 119) was 81.03 mg/dL, Angora (n=5) was 84.20 mg/dL, Persian (n=6) was 81.50 mg/dL and Ragdoll (n=1) was 92 mg/dL. Pearson correlation showed BCS (r=0.403; p= 0.000) had a moderate, positive, and significant correlation with blood glucose concentration in domestic cats. Meanwhile, the results of Kendall's Tau correlation showed that breed (p=0.740) and sex (p=0.555) had insignificant correlation with blood glucose concentration in domestic cats.
Impact of different antibiotic treatments on pregnancy maintenance in Holstein Friesian cows infected with foot-and-mouth disease Vensko Zico Dandi Wahyu Pratama; Jola Rahmahani; Herry Agoes Hermadi; Nusdianto Triakoso; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.34-38

Abstract

This study was conducted at Setia Kawan dairy cattle farming cooperative in Nongkojajar, Pasuruan, East Java to evaluate the effects of different antibiotic treatments on pregnancy sustainability in Holstein Friesian (HF) dairy cows infected with foot and mouth disease (FMD). The objective was to identify which antibiotics have minimal impact on pregnancy outcomes in FMD infected cows. A total of 106 pregnant HF cows infected with FMD were included in this study. Samples were selected using a purposive sampling method with inclusion criteria of pregnant HF dairy cows that tested positive for FMD. Four types of antibiotics were assessed, including enrofloxacin, oxytetracycline, amoxicillin, and penicillin-streptomycin. The results showed that among cows treated with enrofloxacin, 88.46% maintained pregnancy while 11.54% experienced abortion. For oxytetracycline, 73.08% of cows did not abort, whereas 26.92% did. In the amoxicillin group, 61.54% maintained pregnancy and 38.46% aborted. For penicillin-streptomycin, only limited data were available, with 23.07% maintaining pregnancy and no recorded cases of abortion. In conclusion, while variations were observed among different antibiotic treatments, statistical analysis indicated no significant association between antibiotic type and abortion rate in HF cows infected with FMD. Further studies with larger sample sizes are recommended to confirm these findings.
Analisis Keuntungan Kotor Dan Pengaruh Faktor-Faktor Terhadap Hasil Produksi Peternakan Domba Barokah Farm Kediri Dan Para Mitra Rosyta, Pegy; Al-Arif, Mohammad Anam; Ardianto, Ardianto; Mustofa, Imam; Mufasirin, Mufasirin; Triakoso, Nusdianto
Jurnal Ilmiah Fillia Cendekia Vol 10 No 1 (2025): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v10i1.6970

Abstract

Penelitian ini dilakukan untuk mengetahui tingkat tingkat kotor yang diperoleh peternakan domba Barokah Farm Kediri dan mitra serta pengaruh faktor-faktor seperti karakteristik peternak, perkandangan, pemberian pakan, dan perawatan kesehatan ternak terhadap hasil produksi peternakan domba Barokah Farm Kediri dan mitra. Metode penelitian untuk pengumpulan data dilakukan dengan wawancara, kuesioner, dan observasi. Penelitian ini merupakan penelitian deskriptif, dengan jumlah responden sebanyak 12 orang yang terdiri dari peternak domba Barokah Farm Kediri dan 11 mitra. Metode pengumpulan data dilakukan dengan wawancara, kuesioner, dan observasi. Data dari daftar pertanyaan hasil wawancara digunakan untuk analisis keuntungan kotor mengunakan metode full costing dan data dari kuesioner digunakan untuk analisis faktor-faktor terhadap hasil produksi peternakan domba menggunakan Structural Equation Modeling dengan program software Partial Least Square. Hasil penelitian menunjukkan bahwa: (1) analisis kuntungan kotor pada usaha peternakan domba kemitraan Barokah Farm Kediri, mitra menghasilkan keuntungan kotor rata-rata sebesar Rp 136.694.539,- dengan presentase sebesar 44%, dan rata-rata populasi ternak sebanyak 124 ekor domba dengan rata-rata produksi ternak sebanyak 148 ekor domba, (2) faktor yang paling berpengaruh terhadap hasil produksi usaha peternakan domba Barokah Farm Kediri dan mitra adalah faktor karakteristik peternak dengan indikator lama pengalaman beternak, jumlah domba yang dipelihara dan jenis domba yang dipelihara.
Description of antemortem inspection of cattle at the Dimoro slaughterhouse, Blitar City Hasibuan, Sandi Aziz; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Budiarto, Budiarto; Warsito, Sunaryo Hadi; Raharjo, Dadik
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78414

Abstract

The outbreaks of Foot and Mouth Disease (FMD) and Lumpy Skin Disease (LSD) in early 2024 significantly impacted livestock health conditions in Blitar City and led to an increase in emergency slaughter cases. The Dimoro Slaughterhouse plays a crucial role in ensuring the safety of animal-derived food through the implementation of antemortem inspections. This study aims to evaluate the health status of beef cattle and identify pathological conditions detected during antemortem inspections at the Dimoro Slaughterhouse. The research used a quantitative descriptive method with direct observation of 40 cattle during the period of June 13–19, 2025. Clinical examinations were conducted on the head, neck, thorax, abdomen, anus, and limbs. The results showed that most cattle were male (34 heads) and predominantly of the Limousin breed (19 heads). No clinical symptoms of FMD, LSD, fever, or other disorders warranting slaughter rejection were found. Mild findings such as dirt around the anus were recorded in some individuals. Overall, the antemortem inspection was carried out effectively and contributed to ensuring the safety of meat products for public consumption.
Cytotoxicity of Kembang Bulan (Tithonia diversifolia ) Ethanolic Leaf Extract on Rabbit Limbal Mesenchymal Stem Cells Plumeriastuti, Hani; Zahro, Vida Seanita; Triakoso, Nusdianto; Rantam, Fedik Abdul; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Proboningrat, Annise
Media Kedokteran Hewan Vol. 33 No. 3 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i3.2022.233-239

Abstract

Kembang Bulan (Tithonia diversifolia ) is known as an herbal plant that has many useful properties to cure disease, such as diabetes, malaria, and other infections. Instead of much usefulness, T. diversifolia has main biological properties that may induce toxicity in this plant known as sesquiterpene lactones. The aim of this study was to identify the cytotoxicity effect of T. diversifolia leaf extract on limbal mesenchymal stem cells of rabbits using the MTT assay. T. diversifolia leaf extract was divided into 4 concentrations (0.5%, 0.25%, 0.125%, and 0.0625%) and given to limbal mesenchymal stem cells of rabbits in 96-well microplates, which would be incubated for 24 hours. The cytotoxicity result was obtained using the MTT assay method. Every well would be observed to see the cell life level of each concentration. The optical density absorbance was calculated using a microplate reader. T. diversifolia leaf extract with a concentration of 0.0625% has the biggest viability with a level of existence of limbal mesenchymal stem cells of 50%, and the other T. diversifolia leaf extract concentrations of 0.5%, 0.25%, and 0.125% have less than 50% level of existence of limbal mesenchymal stem cells.
Analysis of Differences in Farmer Income Estimation Between Middlemen and Animeter For Bali Cattle in Jaro Subdistrict, Tabalong Regency, South Kalimantan Aulia Rakhman, Muhammad Andika; Soeharsono; Al-Arif, Moh. Anam; Lukiswanto, Bambang Sektiari; Triakoso, Nusdianto; Ma'ruf, Anwar
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.146-156

Abstract

This study investigates differences in farmer income and body weight estimation for male and female Bali cattle in extensive farming in Jaro Subdistrict, Tabalong Regency, South Kalimantan. A random sampling approach was used, involving farmers from nine villages. Statistical analyses revealed a significant difference (p < 0.05) in the body weights of male Bali cattle measured by an animeter compared to those estimated by middlemen. In contrast, no significant difference (p > 0.05) was observed for female Bali cattle. These differences significantly impacted farmer income for male cattle because the income estimated by the animeter exceeded that estimated by the middlemen (p < 0.05). However, no significant difference (p > 0.05) was observed between the two estimation methods for female cattle. In addition, no significant relationship was observed between the estimates made by the middlemen and those made by the animeter for both sexes of Bali cattle. This study used the Monte Carlo simulation to predict demand for male and female Bali cattle in 2024. On average, farmers were able to supply between two and three male Bali cattle and between three to seven female Bali cattle per month. To ensure a steady income, farmers are advised to supply at least one male or female Bali cattle. Moreover, farmer groups are encouraged to compensate for cattle shortages or surpluses, thereby ensuring a stable supply for middlemen and consumers.
The addition of egg yolk to the physiological saline extender improved the motility and viability of kampung rooster spermatozoa at cool temperatures Diba, Lucky; Suzanita Utama; Tjuk Imam Restiadi; Suherni Susilowati; Nusdianto Triakoso; Dwi Wijayanti
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.81-89

Abstract

The purpose of this study was to determine the motility and viability of kampung rooster (Gallus gallus domesticus) spermatozoa in 0.9% Sodium chloride (NaCl) solution as an extender with the addition of egg yolk at different concentrations at cool temperature (5°C). This study was performed using two 1.5 years old healthy local roosters. Semen was collected through massage in the morning. The pooled semen sample was divided into four treatment groups. In the T0 group the semen was diluted in 0.9% NaCl, while in the T1, T2 and T3 groups the semen was diluted in 0.9% NaCl added with 5, 10 and 15% egg yolk. The results showed that the motility and viability of spermatozoa decreased when stored at cool temperatures for ten hours (p <0.05). Semen of roosters stored at 5°C in saline solution without the addition of egg yolks showed the lowest motility and viability of spermatozoa (p <0.05). The addition of egg yolk into the saline extender increased the motility and viability of spermatozoa. Concentration of 15% egg yolk in saline solution resulted in the highest spermatozoa motility and viability when stored for up to 8 hours (p <0.05). However, motility and viability of spermatozoa at 10 hours of storage were not significantly different (p >0.05) with the addition of 10% and 15% egg yolks. Therefore, it could be concluded that the addition of 15% egg yolk into a saline solution as an extender could maintain the motility and viability of kampung rooster spermatozoa when stored at 5°C for 10 hours.
Reproductive Performance of Crossbreed Cows on Lowland Terrain Ardiyansyah, Septiana Alafia Yusi; Sri Agus Sudjarwo; Nusdianto Triakoso; Tri Wahyu Suprayogi; Ismudiono Ismudiono; Oky Setyo Widodo
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.124-130

Abstract

This study aims to identify the differences in reproduction performance of Ongole cross (PO), Limousine cross (Limpo), and Simmental cross (Simpo) in Kerek district, Tuban regency. Primary data was taken by interviewing inseminators and farmers, while secondary data was taken from the Fisheries and Animal Husbandry Department of Tuban regency. The total sample was 360 cows consisting of 30 PO, Limpo, and Simpo cows aged 3, 4, 5, and 6 years respectively which were calculated based on the Roscoe formula. Data was analyzed using the Simple Linear Regression and ANOVA followed by Duncan's multiple range test at a significance level of 95%. The results showed that the services per conception (S/C), conception rate (CR), and calving rate (CvR) were not significantly different (p >0.05) between PO, Limpo and Simpo cows. Meanwhile, days open (DO) and fertility status (FS) of PO cows were higher (p <0.05) than Limpo and Simpo cows. There was no significant difference (p >0.05) in DO and FS between Limpo and Simpo cows. The linear equation with age as a predictor (X) of S/C, DO, and CI (Y) values were not significant (p >0.05) in PO, Limpo, and Simpo cows. It could be concluded that the reproductive performance of PO cows was better than Limpo and Simpo cows.
Co-Authors Agustono, Bodhi Ahmad Thoriqul Firdaus Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Al-Arif, Moh. Anam Annise Proboningrat Ardianto Ardianto Ardiyansyah, Septiana Alafia Yusi Arimbi Arimbi Aulia Rakhman, Muhammad Andika Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Boedi Setiawan Budiarto Budiarto Burhanudin Cahya, Rizka Wulan Chairul Anwar Diba, Lucky Dini, Sayyida Kamila Djoko Galijono DWI WIJAYANTI Erma Safitri Estoepangestie, Agnes Theresia Soelih Faisal Fikri Fedik Abdul Rantam Fransiska, Eunike Melanda Gandul Atik Yuliani Hani Plumeriastuti Hasibuan, Sandi Aziz Herry Agoes Hermadi Hidajati , Nove Hidayatik, Nanik Hisyam, Mirza Atikah Madarina IMAM MUSTOFA Ira Sari Yudaniayanti Ismudiono Ismudiono Ivanalee, Angela Swasti Iwan Sahrial Hamid Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Kuncorojakti, Suryo Legowo, Djoko Lilik Maslachah LINA SUSANTI Lucia Tri Suwanti, Lucia Tri Luthfiyyah Nur Afifah Siswandi Ma'ruf, Anwar Mahdi, Daffa Amanul Maudika Pamela Fiorenza Mirni Lamid Mochamad Lazuardi Mufasirin Muhandis, Putri Jauza Aulia Mustofa Helmi Effendi Niken Hastuti, Apriliwiyani Nunuk Dyah Retno Lastuti Poedji Hastoetik Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Primarizky, Hardany Pujiyanto, Mahendra Putrianirma, Rizki Raharjo, Dadik Rahmadhani, Nurmitasari Rahmahani, Jola Rahmawati, Rr Indah Nur Rao, Sherbrina Bai Seenivasa Rosyta, Pegy Saputro, Amung Logam Sardjana, I Komang Wiarsa Soeharsono Sri Agus Sudjarwo Srimaryanto, Leonardo Reza Sudjono, Budi Santoso Suherni Susilowati Sunarso, Agus Suzanita Utama Taufan Ary Handoko Tjuk Imam Restiadi Tri Wahyu Suprayogi Veena Vasheene Vijaya Kumar Vensko Zico Dandi Wahyu Pratama Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Yudhana, Aditya Yulianti Mauludy Utami Yulianto, Rangga Yunita, Maya Nurwartanti Zahro, Vida Seanita