Article Per Year (5 Year)
p-Index From 2020 - 2025
4.839
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Manajemen dan Agribisnis Zuriat Jurnal Natur Indonesia Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Keteknikan Pertanian Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Buletin Palawija Buletin Penelitian Tanaman Rempah dan Obat Proceeding International Conference on Global Resource Conservation Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Agroteknos Scientific Journal of Informatics Jurnal Agro Bioeksperimen: Jurnal Penelitian Biologi Warta IHP (Warta Industri Hasil Pertanian) BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika Journal of Tropical Crop Science Floribunda Buletin Palma Buletin Penelitian Tanaman Rempah dan Obat Buletin Palawija Agroteksos Jurnal Pusat Inovasi Masyarakat International Journal of Agriculture System Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Sobir Sobir
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

Published : 101 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Journal of Tropical Crop Science

 1 
Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
An Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.172-184

Abstract

Wild banana Musa acuminata ssp. malaccensis, an ancestor of cultivated bananas, possesses valuable genetic diversity, including resistance genes to fusarium wilt, and demonstrates high environmental adaptability. These traits are important for pre-breeding programs, whether by conventional breeding, which is a lengthy process, or by taking advantage of somatic cell manipulation techniques such as somatic hybridization, which requires an efficient plant regeneration system like somatic embryogenesis. We have established an efficient and comprehensive protocol for somatic embryogenesis of this wild Musa using immature zygotic embryo explants covering culture induction and proliferation, somatic embryo development, and subsequent plant conversion. Embryogenic culture was induced on a complex-modified MS medium supplemented with 1 mg.L-1 2,4-D auxin or 5 mg.L-1 picloram. The embryogenic cultures proliferated in the form of granular or nodular structures, which was best obtained by reducing the picloram concentration to 1 mg.L-1 and combining it with the same concentration of 2,4-D at a half-strength macro-nutrient of basal medium. Embryo development from embryogenic cultures and regeneration into shoots. Proembryos as granular structures spontaneously matured into early-stage somatic embryos upon withdrawal of the strong auxin inducer. Increasing the sucrose and gelling agent concentrations in the growth medium improved somatic embryo formation from embryogenic cultures. The frequency of shoot formation from developed somatic embryos was increased by incorporating 0.5 mg.L-1 BA and 0.5 mg.L-1 GA3 into the regeneration medium.
Co-Authors , Kisman , Santoso Achmad Baihaki Adinda Wuriandani Agung Wahyu Susilo Agus Purwito Ahmad Dadang Ahmad Satori Ahmad Yunus Aji Hermawan Alex Hartana Alex Hartana ALEX HARTANA ALFRED P. MANAMBANGTUA Alifiya Herwitarahman Anas Dinurrohman Susila Andi Azhari Andika Septiana Suryaningsih Aris Purwanto Arisanti, Tiffani Nindya Arya Widura Rtionga Astri W Wulandari Astri Windia Wulandari Wulandari Awang Maharijaya Baiq Arriyadul Badi'ah Bonjok Istiaji Budi Santosa C Hanny Wijaya Chesaria, Nanda DADANG, AHMAD Deden Derajat Matra Delvi Maretta Desta Wirnas Didy Sopandie Diny Dinarti Diyah Martanti, Diyah DONATA S. PANDIN Dyra Haryanti Edi Santosa Edi Santosa Edi Santosa Efendi, D. Efendi, Darda Eka Fatmawati Tihurua Eko Priyantono Ellina Mansyah Entit Hermawan Eny Widajati Erni Suminar Euis Nining Evalina C. Pandia Freestina Andarwening Fusao Motoyoshi Gunawan Gunawan Gunawan Gunawan Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hanifah Nuryani Lioe Hanik Rohmah Robi'ah Harmi Andrianyta Heri Harti Herry Suhardiyanto Hidayat , Sri Hendrastuti I Wayan Budiastra Ika Roostika Imam Widodo Iman Rusmana Imas Sukaesih Sitanggang Indah Kurniasari Irvan Faizal Is Helianti Islah Hayati J. K. J. Laisina Joko Prasetiyono Juliati S. Darsa Juliet M.E. Mamahit Ketty Suketi Khairiyah, Hayat Khamidi, Thamrin Kisman Kisman Kristianto Nugroho Kurniawan Rudi Trijatmiko Kuswandi Kuswandi La Ode Safuan La Ode Safuan Laksono Trisnantoro Lilik Pujantoro Lina Herlina Lina Herlina Lina Herlina Lizawati Lizawati M A Chozin M. Syamsul Maarif Machfud Machfud Mahat Magandhi MANAMBANGTUA, ALFRED P. Mardiana - MARIA BINTANG Marlin Marlina Mustafa, Marlina MATHIUS, NURITA TOURAN Matra, Deden Derajat Meddy Rachmadi Memen Surahman Mien A. Rifai Miftahudin . Muchlis . Muhamad Syukur Muhammad Arif Nasution Muhammad Syukur Mukelar Amir Munarti Nazli, Rizal Sjarief Sjaiful Nazly Aswani Nettyani, Naipospos Ni Made Armini Wiendi Nobuo Sugiyama Noorrohmah, Siti Nurita Toruan-Mathius Nurul Khumaida Nurwahyuningsih Nurwahyuningsih OTIH ROSTIANA PANDIN, DONATA S. Parjanto Parjanto Poetri Agustine Aryawati PRASETIYONO, JOKO Prawestri, Apriliana Dyah Priyanti Priyanti Priyanti Priyanti Priyantono, Eko PURNAMA HIDAYAT Purwono Purwono Putra, Fiqhri Mulianda Rahmadara, Gemilang Rahmi Yunianti Ramadhani Dwi Santoso Ramadhani Dwi Santoso Rd. Selvy Handayani Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Ridwan Setiamihardja Risa Aryantri Ritonga, Arya Widura Roberdi , Roedhy Poerwanto Rosmaina Rosmaina S H Hidayat S Noorrohmah Santosa, Budi Santosa, Budi Sarsidi S astrosumarjo Sayekti, Tri Wahono Dyah Ayu Sekar Wulan Prasetyaningtyas Siti Hafsah Siti Hartati Yusida Saragih Soaloon Sinaga SOLIN, NIDA W.N.M. SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sudarmono Sudirman Yahya Sujiprihati, Sriani Sulistijorini Sunyoto Sunyoto Suparjo Suprayanti Martia Dewi Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suryo Wiyono Susan Helmi SYAFRIDA MANUWOTO Syarifah Iis Aisyah Syukur, M Tamami, Djoko TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATIK CHIKMAWATI Tengku Laila Kamaliah Teuku Tajuddin Tias Arlianti Tony Liwang Tri Budiyanti Tri Handayani Tri Muji Ermayanti Tri Muji Ermayanti Trijatmiko, Kurniawan Rudi Trikoesoemaningtyas U Undang Utami Prawati Wida W. Khumaero Widodo Widodo Willy Bayuardi Suwarno Winarso D. Widodo Winda Nawfetrias Witjaksono Wulan Septiningtyas Kurniajati Yoko Mine Yudiwanti Wahyu E. Kusumo Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yusnita Sari Zainal Alim Mas’ud Zumaidar Zumaidar