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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Manajemen dan Agribisnis Zuriat Jurnal Natur Indonesia Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Keteknikan Pertanian Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Buletin Palawija Buletin Penelitian Tanaman Rempah dan Obat Proceeding International Conference on Global Resource Conservation Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Agroteknos Scientific Journal of Informatics Jurnal Agro Bioeksperimen: Jurnal Penelitian Biologi Warta IHP (Warta Industri Hasil Pertanian) BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Indonesian Food Science and TechnologyJournal Jurnal Penelitian Kelapa Sawit Jurnal Agrotek Tropika Journal of Tropical Crop Science Floribunda Buletin Palma Buletin Penelitian Tanaman Rempah dan Obat Buletin Palawija Agroteksos Jurnal Pusat Inovasi Masyarakat International Journal of Agriculture System Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Sobir Sobir
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : Buletin Palma

 1 
Analisis Keragaman 35 Aksesi Kelapa Sawit (Elaeis guineensis Jacq.) Asal BUDI SANTOSA; JOKO PRASETIYONO; AHMAD DADANG; DONATA S. PANDIN; . SOBIR; MEDDY RACHMADI; ALFRED P. MANAMBANGTUA
Buletin Palma Vol 16, No 2 (2015): Desember, 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.006 KB) | DOI: 10.21082/bp.v16n2.2015.183-194

Abstract

ABSTRAK Program pemuliaan untuk peningkatan produksi minyak kelapa sawit dapat dilakukan dengan cara melakukan seleksi plasma nutfah, observasi/eksplorasi lapang, maupun introduksi aksesi baru dari luar negeri. Teknologi seleksi kelapa sawit dapat dilakukan secara konvensional dan non-konvensional. Penelitian ini bertujuan untuk memperoleh informasi keragaman genetik aksesi kelapa sawit asal Kamerun  berdasarkan keragaman karakter morfologi, produksi awal, dengan menggunakan marka molekuler  SSR. Penelitian dilakukan di KP Sitiung dan BB Biogen. Sampel yang dipilih adalah aksesi yang memiliki produksi awal tandan buah segar (TBS) minimal 4 kg.  Sebanyak 35 aksesi kelapa sawit asal Kamerun dan 20 primer SSR digunakan dalam penelitian ini. Hasil pengamatan karakter morfologi menunjukkan bahwa tidak terdapat perbedaan karakter morfologi diantara 35 tanaman kelapa sawit yang produksi awalnya ≥ 4 kg TBS. Produksi awal kelapa sawit berkisar antara 4 - 9,5 kg TBS per tandan dengan koefisien keragaman rendah, yaitu < 20%. Berdasarkan analisis 20 primer SSR dihasilkan   enam pita dengan kisaran 4−12 alel. Jumlah alel dominan sangat mendominasi dibanding dengan alel jarang maupun alel sedang.  Nilai Polymorphism Information Content (PIC) yang diperoleh sebagian bernilai negatif, 11 primer (55%) bernilai positif. Primer MEgcl3639 menghasilkan nilai PIC tertinggi (0,65), sedangkan nilai PIC terendah pada primer MEgcl0046 (-0,48). Berdasarkan analisis UPGMA aksesi 35 kelapa sawit terbagi  ke dalam dua kelompok, yaitu kelompok I terdiri dari 34 aksesi pada tingkat kesamaan genetik 42,5 - 67,5%, dan  kelompok II, yaitu satu aksesi (D91.4). Aksesi  D91.4 memiliki komposisi genetik yang sangat berbeda dengan kelompok I, dapat digunakan sebagai calon tetua persilangan. Perlu penelitian lebih detail pada aksesi D91.4 ini untuk pengamatan karakter kadar minyak, ketebalan cangkang, daging buah, dll.Kata kunci : Kelapa sawit, Kamerun, produksi awal, SSR. Diveristy Analysis of 35 Oil Palm Accessions (Elaeis guineensis Jacq.) Originated from Cameroon Based on Early Production Character by Using SSR Markers ABSTRACT Oil palm breeding programs to increase oil palm production can be done by under going selection in the germplasm collection, observation/exploration in the field and introduction from abroad. The technology can also be done by conventional or non-convention always. This study aimed to obtain information about genetic diversity of palm accessions originated from Cameroon based on the diversity of morphology and early production characters by using SSR molecular markers. Selected samples were those  that had early production of fresh fruit bunches (FFB) of at least 4 kg. The  research were held in Sitiung Field Station and ICABIOGRAD. Accessions used were oil palm accessions  originated from Cameroon as many as 35 accessions, where as microsatellite primers used were as many as 20 SSR  primers. The observation result of morphological characters showed that 35 oil palm accessions with production level of  ≥4,00 kg of FFB were not statically different. Initial production of oil palm accession were ranged from 4,00 to 9,50 kg  FFB per cluster with low diversity coefficient of <20%, which indicating high level of genetic uniformity. Molecular  analysis by using 20 SSR primers resulted  in number of bands up to six, with a range of 4-12 alleles. Dominant alleles  were more dominat as compared to rare or medium alleles. Some of Polymorphism Information Content (PIC) values were  obtained  negative, and only 11 primers (55%) showed positive PIC value. In this study, the primer Megcl3639 produced  the highest PIC value (0.65), while the lowest value was obtained on the primer MEgcl0046 (-0,48). Based on the UPGMA  analysis, 35 oil palm accessions were divided into two groups. The first group was comprised by 34 accessions with similarity of 42,5 - 67,5%, where as group II was comprised by one accession (D91.4). Accession D91.4 has a genetic composition that is very different the group I, can be used as a prospective parent for crosses. More detailed research is needed on this D91.4 accessions such as oil content, shell thickness, thickness of fruit, etc.Keywords: Oil palm, Cameroon, early production, SSR.
Co-Authors , Kisman , Santoso Achmad Baihaki Agung Wahyu Susilo Agus Purwito Ahmad Dadang Ahmad Satori Ahmad Yunus Aji Hermawan Alex Hartana ALEX HARTANA Alex Hartana ALFRED P. MANAMBANGTUA Alifiya Herwitarahman Anas Dinurrohman Susila Andarwening, Freestina Andika Septiana Suryaningsih Aris Purwanto Arisanti, Tiffani Nindya Astri W Wulandari Astri Windia Wulandari Wulandari Awang Maharijaya Azhari, Andi Baiq Arriyadul Badi'ah Bonjok Istiaji Budi Santosa C Hanny Wijaya Chesaria, Nanda DADANG, AHMAD Deden Derajat Matra Delvi Maretta Desta Wirnas Didy Sopandie Diny Dinarti Diyah Martanti, Diyah DONATA S. PANDIN Edi Santosa Edi Santosa Edi Santosa Efendi, D. Efendi, Darda Eka Fatmawati Tihurua Eko Priyantono Ellina Mansyah Entit Hermawan Eny Widajati Erni Suminar Evalina C. Pandia Fadhilah Sitepu, Annisa Fusao Motoyoshi Gunawan Gunawan Gunawan Gunawan Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hanifah Nuryani Lioe Harmi Andrianyta Harti, Heri Haryanti, Dyra Helmi, Susan Hendrastuti Hidayat, Sri Herry Suhardiyanto Hidayat , Sri Hendrastuti I Wayan Budiastra Ika Roostika Imam Widodo Iman Rusmana Imas Sukaesih Sitanggang Inayah Yasmin Kamila Indah Kurniasari Irvan Faizal Is Helianti Islah Hayati J. K. J. Laisina Joko Prasetiyono Juliati S. Darsa Juliet M.E. Mamahit Ketty Suketi Khairiyah, Hayat Khamidi, Thamrin Kisman Kisman Kristianto Nugroho Kurniawan Rudi Trijatmiko Kuswandi Kuswandi La Ode Safuan Laksono Trisnantoro Lilik Pujantoro Lina Herlina Lina Herlina Lina Herlina Lizawati . M A Chozin M. Syamsul Maarif Machfud Machfud Maharijaya, dan Awang Mahat Magandhi MANAMBANGTUA, ALFRED P. Mardiana - MARIA BINTANG Marlin Marlin Marlina Mustafa, Marlina Mas’ud, Zainal Alim MATHIUS, NURITA TOURAN Matra, Deden Derajat Meddy Rachmadi Memen Surahman Mien A. Rifai Miftahudin Miftahudin . Muchlis . Muhamad Syukur Muhammad Arif Nasution Muhammad Syukur Mukelar Amir Munarti Nadila, Dea Nazli, Rizal Sjarief Sjaiful Nazly Aswani Nettyani, Naipospos Ni Made Armini Wiendi Nining, Euis Nobuo Sugiyama Noorrohmah, Siti Nurita Toruan-Mathius Nursalma, Linda Nurul Khumaida Nurwahyuningsih Nurwahyuningsih Olivia Yofananda, Olivia OTIH ROSTIANA PANDIN, DONATA S. Parjanto Parjanto Poetri Agustine Aryawati PRASETIYONO, JOKO Prawestri, Apriliana Dyah Priyanti Priyanti Priyanti Priyanti Priyantono, Eko PURNAMA HIDAYAT Purwono Purwono Putra, Fiqhri Mulianda Putri, Faradila Danasworo Rahmadara, Gemilang Ramadhani Dwi Santoso Ramadhani Dwi Santoso Ramdhani, Cahyati Rd. Selvy Handayani Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Ridwan Setiamihardja Risa Aryantri Ritonga , Arya Widura Ritonga, Arya W Ritonga, Arya Widura Roberdi ,, Roberdi Robi'ah, Hanik Rohmah Roedhy Poerwanto Rosmaina Rosmaina S H Hidayat S Noorrohmah Santosa, Budi Santosa, Budi Sarsidi S astrosumarjo Sayekti, Tri Wahono Dyah Ayu Sekar Wulan Prasetyaningtyas Siti Hafsah Soaloon Sinaga SOLIN, NIDA W.N.M. SRI HENDRASTUTI HIDAYAT SRIANI SUJIPRIHATI Sriani Sujiprihati Sudarmono Sudirman Yahya Sujiprihati, Sriani Sulistijorini Sunyoto Sunyoto Suparjo Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suryo Wiyono SYAFRIDA MANUWOTO Syarifah Iis Aisyah Syukur, M Tamami, Djoko TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATIK CHIKMAWATI Tengku Laila Kamaliah Teuku Tajuddin Tias Arlianti Tony Liwang Toruan-Mathius, dan Nurita Tri Budiyanti Tri Handayani Tri Muji Ermayanti Trijatmiko, Kurniawan Rudi Trikoesoemaningtyas Undang, Undang Utami Prawati Wida W. Khumaero Widodo Widodo Willy Bayuardi Suwarno Winarso D. Widodo Winda Nawfetrias Witjaksono Wulan Septiningtyas Kurniajati Wuriandani, Adinda Yoko Mine Yono, Dwi Yudiwanti Wahyu E. Kusumo Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yusnita Sari, Yusnita Zumaidar Zumaidar