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Journal : Jurnal AgroBiogen

Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots Lina Herlina; Reflinur Reflinur; Kristianto Nugroho; Rerenstradika T. Terryana; Sobir Sobir; Awang Maharijaya; Suryo Wiyono
Jurnal AgroBiogen Vol 14, No 2 (2018): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n2.2018.p65-74

Abstract

Shallot (Allium cepa var. aggregatum) is one of the most important vegetable crops grown in Indonesia. The limited knowledge available on the genetic diversity and the threat of plant disease have been major problems to maintain high shallot production in Indonesia. Development of molecular markers linked to disease resistance is required for molecular breeding activity in this crop. This study aimed to assess the genetic diversity at conserved domain of resistance gene analog (RGA) in a set of 36 Indonesian shallot genotypes to complement morphological characterization. Twelve morphological and fifteen molecular markers traits were investigated in an attempt to characterize and to discriminate the Indonesian shallots genotypes. Characterization at orphological level indicated that phenotypic variance was highest for total bulb weight (TWB, cv = 99.39%) and the least for the plant height (PH, cv = 28.16%). The correlation analysis between traits showed that TWB and number of bulb (NB), TWB and bulb weight per plant (WB), NB and WB, and WB and PH were positively correlated. Molecular analysis revealed a total of 1,512 alleles with an average of 1.946 alleles per locus. The Polymorphism Information Content (PIC) values ranged from 0.253 to 0.676 and six out of 15 RGA markers were highly informative with PIC values ≥0.50. Based on cluster analysis, the 36 Indonesian shallot genotypes were clearly discriminated into six major groups. These results revealed that the RGA-based markers could support the morphological characterization in evaluating the genetic diversity of shallots. 
Resistance Responses of 35 Watermelon Genotypes to Three Isolates of Fusarium oxysporum f. sp. niveum Nazly Aswani; Suryo Wiyono; Sobir Sobir
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p63-74

Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is one of main diseases of watermelon. There have been very limited studies that tested watermelon genotypes to more than one isolates of Fon in Indonesia. This research aimed to determine the resistance of 35 watermelon genotypes to three Fon isolates taken from three different areas in Indonesia. Incubation period (IP) and disease index (DI) of the 35 watermelon genotypes were determined against three Fon isolates collected from Karawang (FK), Lampung (FL), and Purwakarta (FP). The experiment was arranged using a Completely Randomized Design with two replications. DI showed that six watermelon genotypes, i.e. New Hope, Sky Mountain, Southern Light, Super Sweet 66, Uranus, and Yellow Baby demonstrated moderate resistance to resistance phenotypes to all tested Fon isolates. IP and percentage of symptomatic plants (PSP) showed different responses among genotypes either to the same or to different isolates. Genotype New Orchid, for example, showed 57.14% symptomatic plants in less than 10 days after inoculation (DAI) when tested with FL isolate. Meanwhile, when tested with the same isolate, genotype New Dragon showed only 6.67% symptomatic plants in more than 23 DAI. The result of this study indicated that watermelon genotypes showing resistant to all tested isolates should be useful for breeding program to develop watermelon lines with broader resistance spectrum against Fon pathogen. The resistance genotypes selected should also demonstrate good agronomic performances and high yield to be considered as a new watermelon variety.
Co-Authors , Kisman , Santoso Achmad Baihaki Agung Wahyu Susilo Agus Purwito Ahmad Dadang Ahmad Satori Ahmad Yunus Aji Hermawan Alex Hartana ALEX HARTANA Alex Hartana ALFRED P. MANAMBANGTUA Alifiya Herwitarahman Anas Dinurrohman Susila Andarwening, Freestina Andika Septiana Suryaningsih Aris Purwanto Arisanti, Tiffani Nindya Astri W Wulandari Astri Windia Wulandari Wulandari Awang Maharijaya Azhari, Andi Baiq Arriyadul Badi'ah Bonjok Istiaji Budi Santosa C Hanny Wijaya Chesaria, Nanda DADANG, AHMAD Deden Derajat Matra Delvi Maretta Desta Wirnas Didy Sopandie Diny Dinarti Diyah Martanti, Diyah DONATA S. PANDIN Edi Santosa Edi Santosa Edi Santosa Efendi, D. Efendi, Darda Eka Fatmawati Tihurua Eko Priyantono Ellina Mansyah Entit Hermawan Eny Widajati Erni Suminar Evalina C. Pandia Fadhilah Sitepu, Annisa Fusao Motoyoshi Gunawan Gunawan Gunawan Gunawan Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hanifah Nuryani Lioe Harmi Andrianyta Harti, Heri Haryanti, Dyra Helmi, Susan Hendrastuti Hidayat, Sri Herry Suhardiyanto Hidayat , Sri Hendrastuti I Wayan Budiastra Ika Roostika Imam Widodo Iman Rusmana Imas Sukaesih Sitanggang Inayah Yasmin Kamila Indah Kurniasari Irvan Faizal Is Helianti Islah Hayati J. K. J. Laisina Joko Prasetiyono Juliati S. Darsa Juliet M.E. Mamahit Ketty Suketi Khairiyah, Hayat Khamidi, Thamrin Kisman Kisman Kristianto Nugroho Kurniawan Rudi Trijatmiko Kuswandi Kuswandi La Ode Safuan Laksono Trisnantoro Lilik Pujantoro Lina Herlina Lina Herlina Lina Herlina Lizawati . M A Chozin M. Syamsul Maarif Machfud Machfud Maharijaya, dan Awang Mahat Magandhi MANAMBANGTUA, ALFRED P. Mardiana - MARIA BINTANG Marlin Marlin Marlina Mustafa, Marlina Mas’ud, Zainal Alim MATHIUS, NURITA TOURAN Matra, Deden Derajat Meddy Rachmadi Memen Surahman Mien A. Rifai Miftahudin Miftahudin . Muchlis . Muhamad Syukur Muhammad Arif Nasution Muhammad Syukur Mukelar Amir Munarti Nadila, Dea Nazli, Rizal Sjarief Sjaiful Nazly Aswani Nettyani, Naipospos Ni Made Armini Wiendi Nining, Euis Nobuo Sugiyama Noorrohmah, Siti Nurita Toruan-Mathius Nursalma, Linda Nurul Khumaida Nurwahyuningsih Nurwahyuningsih Olivia Yofananda, Olivia OTIH ROSTIANA PANDIN, DONATA S. Parjanto Parjanto Poetri Agustine Aryawati PRASETIYONO, JOKO Prawestri, Apriliana Dyah Priyanti Priyanti Priyanti Priyanti Priyantono, Eko PURNAMA HIDAYAT Purwono Purwono Putra, Fiqhri Mulianda Putri, Faradila Danasworo Rahmadara, Gemilang Ramadhani Dwi Santoso Ramadhani Dwi Santoso Ramdhani, Cahyati Rd. Selvy Handayani Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Ridwan Setiamihardja Risa Aryantri Ritonga , Arya Widura Ritonga, Arya W Ritonga, Arya Widura Roberdi ,, Roberdi Robi'ah, Hanik Rohmah Roedhy Poerwanto Rosmaina Rosmaina S H Hidayat S Noorrohmah Santosa, Budi Santosa, Budi Sarsidi S astrosumarjo Sayekti, Tri Wahono Dyah Ayu Sekar Wulan Prasetyaningtyas Siti Hafsah Soaloon Sinaga SOLIN, NIDA W.N.M. SRI HENDRASTUTI HIDAYAT SRIANI SUJIPRIHATI Sriani Sujiprihati Sudarmono Sudirman Yahya Sujiprihati, Sriani Sulistijorini Sunyoto Sunyoto Suparjo Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suryo Wiyono SYAFRIDA MANUWOTO Syarifah Iis Aisyah Syukur, M Tamami, Djoko TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATIK CHIKMAWATI Tengku Laila Kamaliah Teuku Tajuddin Tias Arlianti Tony Liwang Toruan-Mathius, dan Nurita Tri Budiyanti Tri Handayani Tri Muji Ermayanti Trijatmiko, Kurniawan Rudi Trikoesoemaningtyas Undang, Undang Utami Prawati Wida W. Khumaero Widodo Widodo Willy Bayuardi Suwarno Winarso D. Widodo Winda Nawfetrias Witjaksono Wulan Septiningtyas Kurniajati Wuriandani, Adinda Yoko Mine Yono, Dwi Yudiwanti Wahyu E. Kusumo Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yusnita Sari, Yusnita Zumaidar Zumaidar